WO2005017484A2 - Methods for identifying compounds for regulating muscle mass or function using dopamine receptors - Google Patents
Methods for identifying compounds for regulating muscle mass or function using dopamine receptors Download PDFInfo
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- WO2005017484A2 WO2005017484A2 PCT/US2003/022588 US0322588W WO2005017484A2 WO 2005017484 A2 WO2005017484 A2 WO 2005017484A2 US 0322588 W US0322588 W US 0322588W WO 2005017484 A2 WO2005017484 A2 WO 2005017484A2
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6887—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from muscle, cartilage or connective tissue
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9406—Neurotransmitters
- G01N33/9413—Dopamine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/28—Neurological disorders
- G01N2800/2878—Muscular dystrophy
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/28—Neurological disorders
- G01N2800/2878—Muscular dystrophy
- G01N2800/2885—Duchenne dystrophy
Definitions
- D ⁇ /D S group Drlike
- D 2 / 3 / 4 group D 2 -like
- the D ⁇ lD 5 subclass of receptors signal predominantly by coupling to Gas, leading to the activation of adenylyl cyclase and the formation of cAMP (Gingrich et al., 1993; Missale et al., 1998).
- D1/D 5 receptor subclass has been observed to also couple to G ⁇ o, G ⁇ i, and G ⁇ q indicating that the signal transduction pathways activated by the Gfs subclass may be quite complex (Kimura et al., 1995a; Sidhu et al., 1991; Wang et al., 1995). Dopamine receptors have been cloned from many species including human (Missale et al., 1998).
- agents include anabolic steroids, growth hormone, insulin-like growth factor I, and ⁇ -adrenergic agonists.
- Muscular Dystrophies encompass a group of inherited, progressive muscle disorders, distinguished clinically by the selective distribution of skeletal muscle weakness. The two most common forms of muscle dystrophy are Duchenne and Becker dystrophies, each resulting from the inheritance of a mutation in the dystrophin gene, which is located at the Xp21 locus.
- Symptoms start at about 3-7 years of age with most patients confined to a wheelchair by 10-12 years and many die at about 20 years of age due to respiratory complications.
- Current treatment for Duchenne muscular dystrophy includes administration of prednisone (a corticosteroid drug), which while not curative, slows the decline of muscle strength and delays disability.
- Corticosteroids such as prednisone, are believed to act by blocking the immune cell activation and infiltration which are precipitated by muscle fiber damage resulting from the disease.
- corticosteroid treatment also results in skeletal muscle atrophy which negates some of the potential benefit of blocking the immune response in these patients.
- the present invention relates to the use of Di or D 5 dopamine receptors to identify candidate compounds that are potentially useful in the treatment of skeletal muscle atrophy and or to induce skeletal muscle hypertrophy.
- the Di and D 5 receptors can be used to identify candidate compounds individually or in combination with each other.
- the invention provides in vitro methods for identifying candidate compounds for regulating skeletal muscle mass or function comprising contacting a test compound with a cell expressing Di or D 5 dopamine receptors, or contacting a test compound with isolated Dt or D5 dopamine receptors, and determining whether the test compound either binds to or activates the Di or D 5 dopamine receptors.
- Another embodiment of the invention relates to a method for identifying candidate therapeutic compounds from a group of one or more candidate compounds which have been determined to bind to or activate Di or D 5 dopamine receptors comprising administering the candidate compound to a non-human animal and determining whether the candidate compound regulates skeletal muscle mass or muscle function in the treated animal.
- a further embodiment of the invention relates to a method for identifying candidate compounds for regulating skeletal muscle mass or function comprising, in any order: (i) contacting a test compound with a cell expressing a functional Dt or D5 dopamine receptor, and determining a level of activation of Di or D5 dopamine receptors resulting from the test compound; (ii) contacting a test compound with a cell expressing a functional Di or D 5 dopamine receptor, and determining the level of activation of Di or D 5 resulting from the test compound; followed by (iii) comparing the level of Di or D5 dopamine receptor activation and the level of activation; and (iv) identifying those test compounds that show similar activity toward D ⁇ or D 5 dopamine receptors and or show selectivity for Di or D 5 dopamine receptors as candidate compounds for regulating skeletal muscle mass or function.
- the invention further provides methods for identifying candidate compounds that prolong or augment the agonist-induced activation of Di or D5 dopamine receptors or of a D ⁇ or D 5 dopamine receptor signal transduction pathway. These methods comprise in any order or concurrently: (i) contacting a test compound with a cell which expresses functional Di or D 5 dopamine receptors; (ii) treating the cell with a Di or D5 dopamine receptors agonist for a sufficient time and at a sufficient concentration to cause desensitization of the Di or D 5 dopamine receptors in control cells; followed by (iii) determining the level of activation of D!
- the present invention relates to a method of identifying candidate therapeutic compounds from a group of one or more candidate compounds determined to prolong or augment the activation of Di or D 5 dopamine receptors or activation of Di or D 5 dopamine receptors signal transduction pathway comprising: administering the candidate compound, in conjunction with a Di or D 5 dopamine receptors agonist, to a non-human animal and determining whether the candidate compound regulates skeletal muscle mass or function in the treated animal.
- the invention further provides methods for identifying candidate compounds that increase Di or D 5 dopamine receptor expression comprising contacting a test compound with a cell or cell lysate containing a reporter gene operatively associated with a dopamine receptors gene regulatory element and detecting expression of the reporter gene.
- Test compounds that increase expression of the reporter gene are identified as candidate compounds for increasing Di or D 5 dopamine receptor expression.
- the present invention relates to a method of determining whether those candidate compounds which increase Di or D 5 dopamine receptors expression can be used to regulate skeletal muscle mass or function in vivo by administering a candidate compound to a non-human animal and determining whether the candidate compound regulates skeletal muscle mass or function in the treated animal.
- the present invention also relates to the use of Di or D 5 dopamine receptors agonists, expression vectors encoding a functional Di or D 5 dopamine receptor, expression vectors encoding a constitutively active Di or D 5 dopamine receptors or compounds that increase expression of Di or D 5 dopamine receptors to treat skeletal muscle atrophy.
- the invention provides methods of treating skeletal muscle atrophy, in a subject in need of such treatment, comprising administering to the subject a safe and effective amount of a Dt or D 5 dopamine receptor agonist, an expression vector encoding a functional Di or D 5 dopamine receptor, an expression vector encoding a constitutively active Dj or D 5 dopamine receptor, an expression vector encoding a dopamine receptor or dopamine receptor analog, or a compound that increases expression of Di or D 5 dopamine receptors.
- the present invention relates to a method for treating skeletal muscle atrophy in a subject in need of such treatment comprising administering to the subject a safe and effective amount of a Di or D 5 dopamine receptor agonist in conjunction with a safe and effective amount of a compound that prolongs or augments the agonist-induced activation of Di or D 5 dopamine receptors, or of a Di and D 5 dopamine receptors signal transduction pathway.
- the present invention also relates to the use of a D t or D 5 dopamine receptors agonist to increase skeletal muscle mass or function in a subject.
- FIG. 1 A and IB demonstrates the anti-atrophy effect of the D ⁇ and D 5 dopamine receptor agonists, SKF 81297 (administered subcutaneously, 2X daily), on the tibialis anterior (FIG. 1A) and medial gastrocnemius (FIG. IB) muscles in the mouse sciatic nerve denervation atrophy model.
- FIG. 1A and IB also demonstrate the hypertrophy inducing effect of SKF 81297 on the non-denervated (normal) tibialis anterior (FIG. 1A) and medial gastrocnemius (FIG. IB) muscles.
- FIG. 2 demonstrates the anti-atrophy effect of the Di and D 5 dopamine receptor agonists, SKF 81297 (administered subcutaneously, 2X daily), on casting-induced atrophy of the tibialis anterior muscle and the hypertrophy inducing effect of SKF 81297 on the non- casted (normal) tibialis anterior muscle.
- FIG. 3 demonstrates the anti-atrophy effect of the D ⁇ and D 5 dopamine receptor agonists, fenoldopam (administered subcutaneously, 2X daily), on casting-induced atrophy of the medial gastrocnemius muscle.
- Agonist means any compound, including, but not limited to, antibodies, that activates a receptor.
- dopamine receptor agonists include, but are not limited to, dopamine and dopamine analogs; for example SKF81297 and Fenoldopam.
- Allelic variant means a variant form of a given gene or gene product.
- One of skill in the art recognizes that a large number of genes are present in two or more allelic forms in a population and some genes have numerous alleles.
- Binding affinity means the propensity for a ligand to interact with a receptor and is inversely related to the dissociation constant for a specific dopamine receptor ligand-dopamine receptor interaction.
- the dissociation constant can be measured directly via standard saturation, competition, or kinetics binding techniques or indirectly via pharmacological techniques involving functional assays and endpoints.
- Chimeric antibody means an antibody that contains structural elements from two or more different antibody molecules, i.e., from different animal species. Chimeric antibodies include, but are not limited to, antibodies known as "humanized antibodies” which include, but are not limited to, chimeric antibodies generated by the technique known as complementarity determining region grafting.
- Dopamine receptor agonist means a compound or molecule which has the ability to activate Di or D 5 dopamine receptors, or both. Activation of dopamine receptors can be measured as described hereinafter; using selective agonist such as, SKF 81297 and Fenoldopam ifD ⁇ selective receptor agonists.
- Dopamine receptor means Di or D5 dopamine receptor from any biological species.
- dopamine receptor also includes truncated and/or mutated proteins wherein regions of the receptor molecule not required for ligand binding or signaling have been deleted or modified. For example, one of skill in the art will recognize that a dopamine receptor with one or more conservative changes in the primary amino acid sequence would be useful in the present invention.
- substitution of certain amino acids with different amino acids with similar structure or properties can result in a silent change, i.e., a change that does not significantly alter function.
- Conservative substitutes are well known in the art.
- GPCRs can tolerate substitutions of amino acid residues in the transmembrane alpha-helices, which are oriented toward lipid, with other hydrophobic amino acids, and remain functional.
- Di and D 5 dopamine receptors differing from a naturally occurring sequence by truncations and/or mutations such as conservative amino acid substitutions are also included in the definition of dopamine receptors.
- dopamine receptors from a species other than those listed above, particularly mammalian species would be useful in the present invention.
- One of skill in the art would further recognize that by using probes from the known dopamine receptor species' sequences, cDNA or genomic sequences homologous to the known sequence could be obtained from the same or alternate species by known cloning methods. Such are also included in the definition of and such Di and D 5 dopamine receptors are also included in the definition of Di and D 5 dopamine receptors.
- functional allelic variants or functional splice variants of dopamine receptors might be present in a particular species and that these variants would have utility in the present invention.
- Di and D 5 dopamine receptors variants are also included in the definition of Di and D 5 dopamine receptors.
- Fusions of i and D 5 dopamine receptors polypeptide, or Di and D5 dopamine receptors polypeptide fragment to a non-dopamine receptor polypeptide are referred to as dopamine receptor fusion proteins.
- dopamine receptor fusion proteins Using known methods, one of skill in the art would be able to make fusion proteins of a Di and D 5 dopamine receptors that, while different from native and Di and D 5 dopamine receptors, would remain useful in the present invention.
- non-dopamine receptor polypeptide may be a signal (or leader) polypeptide sequence which co-translationally or post-translationally directs transfer of the protein from its site of synthesis to another site (e.g., the yeast ⁇ -f actor leader).
- the non-dopamine receptor polypeptide may be added to facilitate purification or identification of the dopamine receptor (e.g., poly-His, or Flag peptide).
- Di and D 5 dopamine receptors fusion proteins are also included within the definition of Di and D 5 dopamine receptors.
- Di and D 5 dopamine receptors signal transduction pathway means any signaling pathway (e.g., cAMP, MAP kinase) or combination of signaling pathways that are modulated by the binding of endogenous or exogenous ligands to D t and D 5 dopamine receptors.
- "Fenoldopam” is 6-chloro-2, 3, 4, 5-tetrahydro-l-(4-hydroxyphenyl)-[lH]-3- benzazepine-7, 8-diol, also known as Corlopam®
- “Functional dopamine receptors” refers to dopamine receptors, which bind dopamine receptor agonists in vivo or in vitro and are activated as a result of ligand binding.
- Fusion gene means two or more DNA coding sequences operably associated so as to encode one hybrid protein.
- a “fusion protein” is the protein product of a fusion gene.
- Inhibit means to partially or completely block a particular process or activity. For example, a compound inhibits skeletal muscle atrophy if it either completely or partially prevents muscle atrophy.
- “Operably associated” refers two DNA sequences where the nature of the linkage between the two DNA sequences does not (1) result in the introduction of a frame-shift mutation, (2) interfere with the ability of a promoter region to direct the transcription of the coding sequences, or (3) interfere with the ability of the corresponding RNA transcript to be translated into a protein.
- a coding sequence and regulatory sequences are operably associated when they are covalently linked in such a way as to place the transcription of the coding sequence under the influence or control of the regulatory sequences.
- a promoter region is operably associated with a coding sequence when the promoter region is capable of effecting transcription of that DNA sequence such that the resulting transcript is capable of being translated into the desired protein or polypeptide.
- Perfect identity means the percentage of nucleotides or amino acids that two sequences have in common, calculated as follows. To calculate the percent identity for a specific sequence (the query), the relevant part of the query sequence is compared to a reference sequence using the BestFit comparison computer program, Wisconsin Package, Version 10.1, available from the Genetics Computer Group, Inc.
- Percent identity is calculated with the following default parameters for the BestFit program: the scoring matrix is blosum62.cmp, the gap creation penalty is 8 and the gap extension penalty is 2.
- the relevant part of the query sequence is that which is derived from a dopamine receptor sequence.
- the query is a dopamine receptor/purification tag fusion protein
- only the dopamine receptor polypeptide portion of the sequence is aligned to calculate the percent identity score.
- Polypeptide means any chain of amino acids, regardless of length or post- translational modification (e.g., phosphorylation or glycosylation).
- Promoter means a DNA sequence which controls the initiation of transcription and the rate of transcription from a gene or coding region.
- “Prophylactic treatment” means preventive treatment of a subject, not currently exhibiting signs of skeletal muscle atrophy, in order to completely or partially block the occurrence of skeletal muscle atrophy.
- One of skill in the art would recognize that certain individuals are at risk for skeletal muscle atrophy as discussed in the background section herein.
- one of skill in the art would recognize that if the biochemical changes leading to skeletal muscle atrophy are appropriately regulated, that the occurrence of atrophy would be prevented or reduced in at-risk individuals. For example, muscular dystrophy patients beginning treatment with corticosteroids are at risk for developing skeletal muscle atrophy indicating that prophylactic treatment of such patients would be appropriate.
- “Regulation of skeletal muscle mass or function” includes regulation of skeletal muscle mass, skeletal muscle function or both.
- "Regulatory element” means a DNA sequence that is capable of controlling the level of transcription from an operably associated DNA sequence. Included within this definition of regulatory element are promoters and enhancers.
- a dopamine receptor gene regulatory element is a DNA sequence capable of controlling the level of transcription from the dopamine receptor gene.
- Reporter gene means a coding sequence whose product can be detected, preferably quantitatively, wherein the reporter gene is operably associated with a heterologous promoter or enhancer element which is responsive to a signal which is to be measured.
- the promoter or enhancer element in this context is referred to herein as a "responsive element”.
- SPF 81297 is 6-chloro-7, 8-dihydroxyl-l-phenyl-2, 3, 4, 5-tetrahydro-lH-3- benzazepine purchased from RBI/Sigma, Natick, MA.
- Selective agonist means that the agonist has significantly greater activity toward a certain receptor(s) compared with other receptors, not that it is completely inactive with regard to other receptors.
- Di and D 5 dopamine receptor selective agonists are not limited to SKF 81297 and Fenoldopam.
- SKF means SmithKline French
- Sketal muscle hypertrophy means an increase in skeletal muscle mass or skeletal muscle function or both.
- Sketal muscle atrophy means the same as “muscle wasting” and means a decrease in skeletal muscle mass or skeletal muscle function or both.
- Splice variant means a mRNA or protein which results from alternative exon usage.
- a mRNA may be expressed in a different form, as a splice variant, and thus the translated protein will be different depending upon the mRNA that is expressed.
- a “therapeutically effective amount” of a substance is an amount capable of producing a medically desirable result in a treated patient, e.g., decreases skeletal muscle atrophy, increases skeletal muscle mass or increases skeletal muscle function, with an acceptable benefit: risk ratio; in a human or non-human mammal.
- “Therapeutic treatment” means treatment of a subject in which an increase in muscle mass or muscle function is desirable. For example, treatment of a subject currently exhibiting signs of skeletal muscle atrophy in order to partially or completely reverse the skeletal muscle atrophy that has occurred or to completely or partially block the occurrence of further skeletal muscle atrophy would be therapeutic treatment of that subject.
- therapeutic treatment also includes, for example, treatment of a subject not exhibiting signs of skeletal muscle atrophy to induce skeletal muscle hypertrophy, e.g., treatment of a livestock animal to increase muscle mass.
- treatment means prophylactic or therapeutic treatment.
- FIG. 2 demonstrates the anti-atrophy effect of the Di and D 5 dopamine receptor agonists, SKF 81297 (administered subcutaneously, 2X daily), on casting-induced atrophy of the tibialis anterior muscle and the hypertrophy inducing effect of SKF 81297 on the non- casted (normal) tibialis anterior muscle.
- FIG. 3 demonstrates the anti-atrophy effect of the Di and D5 dopamine receptor agonists, fenoldopam (administered subcutaneously, 2X daily), on casting-induced atrophy of the medial gastrocnemius muscle.
- Figure 1 shows that SKF 81297 inhibits denervation-induced atrophy of the tibialis anterior (FIG. 1A) medial gastrocnemius (FIG. IB) muscles in a mouse sciatic nerve denervation atrophy model.
- FIG.l demonstrates that SKF 81297 induces hypertrophy in the normal (non-denervated) tibialis anterior (FIG. 1A) and medial gastrocnemius (FIG. IB) muscles.
- SKF 81297 inhibits disuse-induced atrophy of the tibialis anterior muscle.
- statistically significant hypertrophy of the tibialis anterior muscles of the non-casted leg was also observed with SKF 81297 treatment.
- a - physiological saline (control) B - SKF 81297 (0.3 mg/kg) + theophylline; C - SKF 81297 (1.0 mg/kg) + theophylline; D - SKF 81297 (3.0 mg/kg) + theophylline; * - p ⁇ 0.05 compared to saline.
- mice were injected subcutaneously in the midscapular region twice daily, with SKF 81297 or vehicle control (physiological saline) for ten days at the daily delivered dose indicated.
- SKF 81297 was co-administered with twice daily intra-peritoneal dosing of the phosphodiesterase inhibitor theophylline (30 mg/kg).
- the tibialis anterior muscle was removed and weighed to determine the degree of atrophy.
- Figure 3 (FIG. 3.) demonstrates that fenoldopam inhibits disuse-induced atrophy of the medial gastrocnemius muscle.
- mice were injected subcutaneously in the midscapular region twice daily, with fenoldopam or vehicle control (physiological saline) for ten days at the daily delivered dose indicated. On day ten, the medial gastrocnemius muscle was removed and weighed to determine the degree of atrophy.
- Preparation of dopamine receptors, dopamine receptor or dopamine receptor analogs, or cell lines expressing dopamine receptors Di and D 5 dopamine receptors can be prepared for a variety of uses, including, but not limited to, the generation of antibodies, use as reagents in the screening assays of the present invention, and use as pharmaceutical reagents for the treatment of skeletal muscle atrophy. It will be clear to one of skill in the art that, for certain embodiments of the invention, purified polypeptides will be most useful, while for other embodiments cell lines expressing the polypeptides will be most useful.
- the cells which express functional dopamine receptors.
- the source of dopamine receptors is a cell line expressing the polypeptide
- the cells may, for example, endogenously express dopamine receptor, have been stimulated to increase endogenous dopamine receptor expression or have been genetically engineered to express a dopamine receptor.
- Methods for determining whether a cell line expresses a polypeptide of interest are known in the art, for example, detection of the polypeptide with an appropriate antibody, use of a DNA probe to detect mRNA encoding the protein (e.g., northern blot or PCR techniques), or measuring binding of an agent selective for the polypeptide of interest (e.g., a radiolabeled selective agonist).
- detection of the polypeptide with an appropriate antibody e.g., northern blot or PCR techniques
- an agent selective for the polypeptide of interest e.g., a radiolabeled selective agonist.
- the use of recombinant DNA technology in the preparation of Di and D 5 dopamine receptors, or of cell lines expressing these polypeptides is particularly contemplated. Such recombinant methods are well known in the art.
- Di and D 5 dopamine receptors an expression vector that comprises a nucleic acid which encodes the polypeptide of interest under the control of one or more regulatory elements, is prepared.
- Genomic or cDNA sequences encoding and Di and D 5 dopamine receptors from several species have been described and are readily available from the GenBank database (available at ⁇ http://www.ncbi.nlm.nih.gov/>) or Derwent database (available at ⁇ http://www.derwent.co.uk/geneseq/index.html>) as well as in the sequence listing for this application.
- GenBank database available at ⁇ http://www.ncbi.nlm.nih.gov/>
- Derwent database available at ⁇ http://www.derwent.co.uk/geneseq/index.html>
- the accession numbers for and Di and D 5 dopamine receptors sequences and corresponding SEQ ID NOS. are shown in Table I.
- one means of isolating a nucleic acid molecule encoding a Di and D 5 dopamine receptor is to screen a genomic DNA or cDNA library with a natural or artificially synthesized DNA probe, using methods well known in the art, e.g., by PCR amplification of the sequence from an appropriate library.
- Another method is to use oligonucleotide primers specific for the receptor of interest to PCR amplify the cDNA directly from mRNA isolated from a particular tissue (such as skeletal muscle). Such isolated mRNA is commercially available.
- nucleic acid probes corresponding to portions of the known dopamine receptor sequences the homologous cDNAs or genomic sequences from other species can be obtained using known methods.
- Particularly useful in the methods of the present invention are dopamine receptors from the species including, but not limited to, human, mouse, rat, pig, monkey, chimpanzee, marmoset, dog, cow, sheep, cat, chicken and turkey.
- the isolated nucleic acid molecule encoding the dopamine receptor of interest is then ligated into a suitable expression vector.
- the expression vector thus prepared, is expressed in a host cell and the host cells expressing the receptor are used directly in a screening assay or the receptor is isolated from the host cells expressing the receptor and the isolated receptor is used in a screening assay.
- the host-expression vector systems that may be used for purposes of the invention include, but are not limited to: microorganisms such as bacteria (e.g., E. coli, B.
- subtilis transformed with recombinant bacteriophage DNA, plasmid DNA, or cosmid DNA expression vectors containing dopamine receptor nucleotide sequences; yeast (e.g., Saccharomyces, Pichia) transformed with recombinant yeast expression vectors containing dopamine receptor nucleotide sequences; insect cell systems infected with recombinant virus expression vectors (e.g., baculovirus) containing dopamine receptor nucleotide sequences; plant cell systems infected with recombinant virus expression vectors (e.g., cauliflower mosaic virus, tobacco mosaic virus) or transformed with recombinant plasmid expression vectors (e.g., Ti plasmid) containing dopamine receptor nucleotide sequences; or mammalian cell systems (e.g., COS, CHO, HEK293, NLH3T3) harboring recombinant expression constructs containing promoters derived from the genome of mammalian
- the host cell is used to produce the polypeptide of interest. Because the dopamine receptor is a membrane bound molecule, it is purified from the host cell membranes or the dopamine receptor is utilized while anchored in the cell membrane, i.e., whole cells or membrane fractions of cells are used. Purification or enrichment of the dopamine receptors from such expression systems is accomplished using appropriate detergents and lipid micelles by methods well known to those skilled in the art. In bacterial systems, a number of expression vectors may be advantageously selected depending upon the use intended for the gene product being expressed. For example, when a large quantity of such protein is produced for the generation of antibodies to dopamine receptors, vectors which direct the expression of high levels of protein products are desirable.
- baculovirus A. californica nuclear polyhedrosis virus AcNPV
- baculovirus A. californica nuclear polyhedrosis virus AcNPV
- S. frugiperda cells S. frugiperda cells
- dopamine receptor nucleotide sequences are cloned into non-essential regions of the virus and placed under the control of an AcNPV promoter.
- the recombinant viruses are then used to infect cells in which the inserted gene is expressed and the protein is purified by one of many techniques known to one skilled in the art.
- a number of viral-based expression systems may be utilized. Utilization of these expression systems often requires the creation of specific initiation signals in the vectors for efficient translation of the inserted nucleotide sequences. This is particularly important if a portion of the dopamine receptor gene is used which does not contain the endogenous initiation signal.
- the placement of this initiation signal, in frame with the coding region of the inserted nucleotide sequence, as well as the addition of transcription and translation enhancing elements and the purification of the recombinant protein, are achieved by one of many methodologies known to one skilled in the art. Also important in mammalian host cells is the selection of an appropriate cell type which is capable of the necessary post translational modifications of the recombinant protein.
- Such modifications for example, cleavage, phosphorylation, glycosylation, etc., require the selection of the appropriate host cell which contains the modifying enzymes.
- host cells include, but are not limited to, CHO, HEK293, NLH3T3, COS, etc. and are known by those skilled in the art.
- stable expression is preferred.
- cell lines that stably express dopamine receptors may be engineered.
- electroporation, calcium phosphate transfection, or liposome-mediated transfection can generate a cell line that stably expresses dopamine receptors.
- appropriate expression control elements e.g., promoter sequences, enhancer sequences, transcriptional termination sequences, polyadenylation sites, translational start sites, etc.
- the selectable marker may either be contained within the same vector, as the gene of interest, or on a separate vector, which is co-transfected with the dopamine receptor sequence containing vector.
- the selectable marker in the expression vector may confer resistance to the selection and allows cells to stably integrate the vector into their chromosomes and to grow to form foci which in turn can be cloned and expanded into cell lines.
- the selectable marker is herpes simplex virus thymidine kinase, hypoxanthine-guanine phosphoribosyltransferase or adenine phosphoribosyltransferase
- the appropriate cell type would be tk-, hgprt- or aprt- cells, respectively.
- normal cells can be used where the selectable marker is dhfr, gpt, neo or hygro which confer resistance to methotrexate, mycophenolic acid, G-418 or hygromycin, respectively.
- Such recombinant cell lines are useful for identification of candidate compounds that affect the dopamine receptor activity.
- Antibodies that selectively recognize one or more epitopes of a dopamine receptor are also encompassed by the invention.
- Such antibodies include, e.g., polyclonal antibodies, monoclonal antibodies, chimeric antibodies, human antibodies, single chain antibodies, Fab fragments, F(ab') 2 fragments, molecules produced using a Fab expression library, human antibodies (polyclonal or monoclonal) produced in transgenic mice and epitope binding fragments of any of the above.
- chimeric or human antibodies are preferred; human antibodies are most preferred.
- the antibodies can be utilized in conjunction with the compound screening schemes described herein for the evaluation of test compounds, e.g., for immobilization of dopamine receptor polypeptides or such antibodies can be used in conjunction with gene therapy techniques to evaluate, for example, the expression of dopamine receptors either in cells or directly in patient tissues in which these genes have been introduced.
- antibodies of the present invention are useful in the treatment of skeletal muscle atrophy.
- Antibodies selective for the dopamine receptor can be screened by the methods of the present invention to identify a subset of the antibodies that are dopamine receptor agonists.
- anti-idiotype antibodies generated against antibodies specific for dopamine receptor may be useful as dopamine receptor agonists and like anti-dopamine receptor antibodies may be screened for their ability to activate the dopamine receptor by methods of the present invention.
- a variety of host animals may be immunized by injection with dopamine receptors, anti-dopamine receptor antibody, or immunogenic fragments thereof by methods well known in the art.
- the immunogen is an anti-dopamine receptor antibody. Production of anti- idiotype antibodies is described, for example, in US Patent No. 4,699,880, incorporated herein by reference.
- Suitable host animals include, but are not limited to, rabbits, mice, goats, sheep and horses.
- Immunization techniques are well known in the art. Polyclonal antibodies can be purified from the serum of the immunized animals, or monoclonal antibodies can be generated by methods that are well known in the art. These techniques include, but are not limited to, the well-known hybridoma techniques of Kohler and Milstein, human B-cell hybridoma techniques, and the EBV hybridoma technology. Monoclonal antibodies may be of any immunoglobulin class, including IgG, IgE, IgM, IgA, and IgD containing either kappa or lambda light chains. Because of the immunogenicity of non-human antibodies in humans, chimeric antibodies are preferred to non-human antibodies when used for therapeutic treatment of human patients.
- the transgenic mice are immunized in the normal fashion with a selected antigen, e.g., all or a portion of Di and D 5 dopamine receptors.
- Monoclonal antibodies directed against the antigen are obtained using conventional hybridoma technology from these immunized transgenic mice. This technology is described in detail in U.S. Pat. Nos. 5,874,299; 5,877,397; 5,569,825; 5,661,016; 5,770,429; and 6,075,181, all incorporated herein by reference.
- the hybridoma cells can be used as a source of rearranged heavy chain and light chain loci for subsequent expression or genetic manipulation.
- the recovered rearranged loci can be manipulated as desired.
- the constant region can be eliminated or exchanged for that of a different isotype or the variable regions can be linked to encode single chain Fv regions.
- Such techniques are described in WO 96/33735 and WO 96/34096, all incorporated herein by reference.
- Test Compounds that can be screened in accordance with the assays of the invention include but are not limited to, libraries of known compounds, including natural products, such as plant or animal extracts, synthetic chemicals, biologically active materials including proteins, peptides such as soluble peptides, including but not limited to members of random peptide libraries and combinatorial chemistry derived molecular library made of D- or L- configuration amino acids, phosphopeptides (including, but not limited to, members of random or partially degenerate, directed phosphopeptide libraries), antibodies (including, but not limited to, polyclonal, monoclonal, chimeric, human, anti- idiotypic or single chain antibodies, and Fab, F(ab') 2 and Fab expression library fragments, and epitope-binding fragments thereof), organic and inorganic molecules.
- libraries of known compounds including natural products, such as plant or animal extracts, synthetic chemicals, biologically active materials including proteins, peptides such as soluble peptides, including but not limited to members of random
- Dopamine receptors are GPCRs, and thus knowledge of the dopamine receptor protein sequence allows for the generation of a model of its binding site that can be used to screen for potential ligands. This process can be accomplished in several manners well known in the art.
- the most robust approach involves generating a sequence alignment of the dopamine receptor sequence to a template (derived from the bacterio-rhodopsin or rhodopsin crystal structures or other GPCR model), conversion of the amino acid structures and refining the model by molecular mechanics and visual examination. If a strong sequence alignment cannot be obtained, then a model may also be generated by building models of the hydrophobic helices. These are then fitted together by rotating and translating each helix relative to the others starting from the general layout of the known rhodopsin structures. Mutational data that point towards residue-residue contacts may also be used to position the helices relative to each other so that these contacts are achieved.
- the model may be completed by refinement using molecular mechanics and loop building of the intracellular and extracellular loops using standard homology modeling techniques.
- General information regarding GPCR structure and modeling can be found in Schoneberg, T. et. al., Molecular and Cellular Endocrinology, 151:181-193 (1999), Flower, D., Biochimica et Biophysica Acta, 1422:207-234 (1999), and Sexton, P.M., Current Opinion in Drug Discovery and Development, 2(5):440-448 (1999).
- FLEXX is best used to confirm DOCK suggestions, or to examine libraries of compounds that are generated combinatorially from known ligands or templates.
- Di and D 5 dopamine receptors play a role in regulating skeletal muscle atrophy hypertrophy enables various methods of screening one or more test compounds to identify candidate compounds that ultimately may be used for prophylactic or therapeutic treatment of skeletal muscle atrophy.
- This invention provides methods for screening test compounds for their ability to bind to Di and D 5 dopamine receptors, activate Di and D 5 dopamine receptors, prolong or augment the agonist-induced activation of Di and D 5 dopamine receptors or of a Di and D 5 dopamine receptor signal transduction pathway or increase expression of Di and D 5 dopamine receptor genes.
- the assays described above should be conducted using a cell, or membrane from a cell, which expresses only Di and D5 dopamine receptors or the assays can be conducted with a recombinant source of Di and D 5 dopamine receptors.
- Cells expressing both forms of dopamine receptor may be modified using homologous recombination to inactivate or otherwise disable one of the dopamine receptor genes.
- the source of dopamine receptor contains more than one dopamine receptor type, the background signal produced by the receptor, which is not of interest, must be subtracted from the signal obtained in the assay.
- Such compounds may be used, for example, in conjunction with a Di or D 5 dopamine receptor agonist for the treatment of skeletal muscle atrophy.
- the method uses a cell based assay comprising in any order or concurrently (i) contacting the cells with a test compound; (ii) treating cells expressing functional Di and D 5 dopamine receptors with Di and D 5 dopamine receptor agonists at a concentration of agonist and for a period of agonist-receptor exposure sufficient to allow desensitization of the receptor; followed by (iii) determimng the level of activation of the Di and D 5 dopamine receptors.
- receptor desensitization includes, but not limited to, receptor phosphorylation, receptor internalization or degradation and dopamine receptor signal transduction pathway down- modulation.
- One of skill in the art can determine the appropriate time (i.e., before, during or after agonist treatment) for contacting the cells with the test compounds depending upon which mechanism of desensitization is targeted. For example, contacting the cells with test compounds following agonist treatment, can detect test compounds which block receptor desensitization which occurs as a result of phosphorylation of the receptor.
- the invention provides a method of screening one or more test compound to identify candidate compounds which regulate transcription from the Di and D 5 dopamine receptor gene or regulate Di and D 5 dopamine receptor expression.
- Candidate compounds which regulate Di and D5 dopamine receptor expression can also be identified in a method wherein a cell is contacted with a test compound and the expression of dopamine receptor is determined. The level of expression of Di and D 5 dopamine receptors in the presence of the test compound is compared with the level of expression in the absence of the test compound. Test compounds which increase the expression of Di and D 5 dopamine receptors are identified as candidate compounds for increasing muscle mass or muscle function. Such a method detects candidate compounds which increase the transcription or translation of the Di and D 5 dopamine receptors or which increase the stability of the mRNA or Di and D 5 dopamine receptor protein.
- VH Screening of candidate compounds using models of skeletal muscle atrophy
- Candidate compounds selected from one or more test compounds by an in vitro assay, as described above, can be further tested for their ability to regulate skeletal muscle mass or function in model systems of skeletal muscle atrophy and/or hypertrophy.
- Such models of skeletal muscle atrophy or hypertrophy include both in vitro cell culture models and in vivo animal models of skeletal muscle atrophy.
- Such additional levels of screening are useful to further narrow the range of candidate compounds that merit additional investigation, e.g., clinical trials.
- the candidate compounds are administered to non-human animals and the response of the animals is monitored, for example, by assessing changes in markers of atrophy or hypertrophy such as: skeletal muscle mass, skeletal muscle function, muscle or myofiber cross-sectional area, contractile protein content, non-contractile protein content or a biochemical or genetic marker that correlates with skeletal muscle mass or function changes.
- markers of atrophy or hypertrophy such as: skeletal muscle mass, skeletal muscle function, muscle or myofiber cross-sectional area, contractile protein content, non-contractile protein content or a biochemical or genetic marker that correlates with skeletal muscle mass or function changes.
- candidate therapeutic compounds which induce skeletal muscle hypertrophy or prevent any aspect of skeletal muscle atrophy should be considered as prospective therapeutic candidates for treatment of human skeletal muscle atrophy, and are referred to herein as candidate therapeutic compounds.
- undesirable side effects such as toxicity may also be detected in such a screen.
- Models of cachexia- induced atrophy include, for example, inoculation of an animal with tumorigenic cells with cachexia forming potential, infection of an animal with infectious agents (such as viruses which cause AIDS) which result in cachexia and treatment of an animal with hormones or cytokines such as CNTF, TNF, IL-6, IL-1, etc. which induce cachexia.
- Models of heart failure-induced atrophy include the manipulation of an animal so that heart failure occurs with concomitant skeletal muscle atrophy.
- Neurodegenerative disease-induced atrophy models include autoimmune animal models such as those resulting from immunization of an animal with neuronal components.
- the extent of atrophy in the affected muscles is analyzed as described above for the denervation model.
- the leg casting disuse atrophy model involves casting one hind leg of an animal from the knee down through the foot. Typically, muscle mass is reduced 20-40% after ten days of casting.
- test compounds are administered by injection or by continuous infusion via implantation of an osmotic minipump (e.g., Alzet, Palo Alto, CA) to determine their effect on leg casting induced skeletal muscle atrophy.
- an osmotic minipump e.g., Alzet, Palo Alto, CA
- vectors include, but are not limited to, adenovirus, adenovirus associated virus, retrovirus and herpes virus vectors in addition to other particles that introduced DNA into cells (e.g., liposome, gold particles, etc.) or by direct injection of the DNA expression vector, containing the gene of interest, into human tissue (e.g., muscle).
- Candidate compounds or candidate therapeutic compounds identified by screening methods described herein can be administered to individuals to treat skeletal muscle atrophy, or to induce skeletal muscle hypertrophy.
- the present invention encompasses methods and compositions for modulating skeletal muscle atrophy, including, but not limited to, skeletal muscle atrophy induced by disuse due to surgery, bed rest, broken bones; denervation/nerve damage due to spinal cord injury; autoimmune disease; infectious disease; glucocorticoid use for unrelated conditions; sepsis due to infection or other causes; nutrient limitation due to illness or starvation; cancer cachexia; chronic inflammation; AIDS cachexia; COPD; congestive heart failure; sarcopenia and genetic disorders; e.g., muscular dystrophies, neurodegenerative diseases.
- Agonists of Di and D 5 dopamine receptors can be used to inhibit skeletal muscle atrophy. It is not necessary that effective compounds demonstrate absolute specificity for dopamine receptor. It is contemplated that specific antagonist of other affected receptors can be co- administered with an effective, but nonspecific, agonist. Alternately, this lack of specificity may be addressed by modulation of dose alone, or the dosing regimen.
- the candidate compounds or candidate therapeutic compounds identified by the screening methods of the present invention may be administered in conjunction with compounds which prolong or augment the activation of a Di and D5 dopamine receptors or of a Di and D 5 dopamine receptors signal transduction pathway.
- the compounds for use according to the present invention may be delivered by, but not limited to, the following forms: liquid, powder, gel or in the form of an aerosol spray utilizing either pressurized or non-pressurized propellants in either premeasured or non-premeasured doses.
- the pharmacologically active compound may be formulated with appropriate fillers, vehicles, preservatives, buffers, etc.
- the pharmacologically active compound may be formulated with acceptable physiological carriers, preservatives, etc. and be prepared as suspensions, solutions, emulsion, powders ready for constitution, etc. for either bolus injection or infusion. Doses of these compounds may be administered by a variety of technologies including hypodermic needles, high-pressure devices, etc.
- Di and D 5 dopamine receptors can be employed not only in basic drug screening, but also in clinical trials.
- the effectiveness of a compound determined by a screening assay to increase Di and D 5 dopamine receptor activity or Di and D 5 dopamine receptor expression can be assessed in clinical trials of patients with, or at risk for, skeletal muscle atrophy.
- the effect of the compound on the patient can be determined, for example, by observing the change in skeletal muscle mass, skeletal muscle function, biochemical markers of muscle breakdown or quality of life measures.
- muscle protein loss as a result of muscle atrophy can be measured by quantitating levels of amino acids or amino acids derivatives, i.e., 3-methyl histidine, in the urine or blood of a subject.
- Quality of life measures include, but are not limited to, the ease of getting out of a chair, number of steps taken before tiring or ability to climb stairs.
- the stably transfected cells (HEK293/CRE- LUC/pLRESneo/ Di and D 5 dopamine receptors cells) are propagated in DMEM (Life Technologies, Rockville, MD) containing 10% fetal bovine serum (Clonetech Inc., Palo Alto, CA, USA), penicillin/streptomycin solution (Life Technologies, Rockville, MD), L- glutamine (Life Technologies, Rockville, MD), and non-essential amino acid (Life Technologies, Rockville, MD) at 37°C in a 5% carbon dioxide/95% air atmosphere.
- the clones are characterized for both dopamine receptor binding and CRE-LUC activation following exposure to dopamine receptor as described in Example 2 and Example 3. Cells expressing the Di and D 5 dopamine receptor at an appropriate level and which are appropriately coupled to the CRE-LUC reporter system are then utilized for further analysis.
- a concentration of SCH23390 is added which is half maximal, in terms of binding, in addition to varying concentrations of the compound of interest.
- Example 3 Receptor Activation Assay Receptor activation analysis is performed by seeding the HEK293/CRE-LUC/pIRESneo/ Di or D 5 dopamine receptors cells of Example 1 into Packard View Plate-96 (Packard Inc., CA). Cells are seeded in DMEM medium containing 10% fetal bovine serum, penicillin/streptomycin solution, L-glutamine, and non-essential amino acid at 37°C in a 5% carbon dioxide/95% air atmosphere and incubated overnight.
- the medium is then removed and replaced with DMEM (Life Technologies, Rockville, MD) containing 0.01% bovine albumin fraction V (SIGMA, St. Louis, MO) containing the compound of interest.
- DMEM Life Technologies, Rockville, MD
- bovine albumin fraction V SIGMA, St. Louis, MO
- the cells are then incubated for four hours at 37°C in a 5% carbon dioxide/95% air atmosphere after which the medium is removed and the cells are washed twice with Hanks Balanced Salt Solution (Life Technologies, Rockville, MD). Lysis Reagent (Promega Inc., Madison, WI) is then added to the washed cells and the cells are incubated for 20 minutes at 37°C in a 5% carbon dioxide/95% air atmosphere.
- Example 4 Screen to identify candidate compounds that prolong or augment the activation of Di or D 5 dopamine receptors and/or a Di and D 5 dopamine receptor signal transduction pathway. Identification of compounds that prolong or augment the agonist-induced activation of the Di or D 5 dopamine receptors or of a Di and D5 dopamine receptors signal transduction pathway, involves a variation of the Receptor Activation Assay described in Example 3. Specifically, this assay is performed by seeding the HEK293/ CRE-LUC/pIRESneo D ⁇ and D 5 dopamine receptor cells into Packard View Plate-96 (Packard Inc., CA).
- DMEM fetal bovine serum
- penicillin/streptomycin solution penicillin/streptomycin solution
- L-glutamine L-glutamine
- non-essential amino acid a 5% carbon dioxide/95% air atmosphere
- DMEM fetal bovine serum
- SIGMA bovine albumin fraction V
- SKF81297 a bovine albumin fraction V
- the cells are then incubated for four hours at 37°C in a 5% carbon dioxide/95% air atmosphere after which the medium is removed and the cells are washed twice with Hanks Balanced Salt Solution (Life Technologies, Rockville, MD). Lysis Reagent (Promega Inc., Madison, WI) is then added to the washed cells and the cells are incubated for 20 minutes at 37°C in a 5% carbon dioxide/95% air atmosphere. The cells are then placed at -80°C for 20 minutes followed by a 20 minute incubation at 37°C in a 5% carbon dioxide/95% air atmosphere.
- Lysis Reagent Promega Inc., Madison, WI
- Luciferase Assay Buffer and Luciferase Assay Substrate are added to the cell lysates and luciferase activity is quantitated using a luminometer.
- Test compounds which stimulate fluorescence significantly above the levels of control untreated cells, after correction for variations in cell density, are considered candidate compounds for regulating skeletal muscle mass or function.
- the compounds of most interest are those that induce relatively higher levels of fluorescence.
- Example 5 Screens to identify candidate compounds that increase Di or D 5 dopamine receptor expression
- the sequence containing the promoter region of the Di or D5 dopamine receptor genes, beginning far enough upstream of the transcriptional initiation site to contain all the regulatory elements necessary for physiological expression of the Di or D5 dopamine receptor genes in the appropriate tissue is retrieved from the human genome database.
- Two oligonucleotides, one containing the 5' end of the promoter region (5' oligonucleotide) and one containing the 3' end of the promoter region including the transcriptional start site (3' oligonucleotide) are synthesized.
- oligonucleotides also contain restriction endonuclease sites which are not present in the Di or D5 dopamine receptor genes regulatory region with one unique site in the 5' oligonucleotide and a different unique restriction endonuclease site in the 3' oligonucleotide.
- the 5' and 3' oligonucleotides are used for PCR amplification of the Di or D 5 dopamine receptor genes regulatory region from human DNA (Clonetech Inc., Palo Alto, CA, USA) using the PCR kit, Advantage®Genomic PCR kit (Clonetech Inc., Palo Alto, CA, USA).
- the Di and D 5 dopamine receptor genes regulatory region PCR products are purified from PCR artifacts by agarose gel electrophoresis and the Di and D5 dopamine receptor genes regulatory region DNA fragment is purified from the agarose gel using a purification product such as NucleoTrap (Clonetech Inc., Palo Alto, CA, USA).
- Cloning of the Di and D 5 dopamine receptor genes regulatory region PCR products into the pECFP-1 vector is accomplished by first cutting the Di and D 5 dopamine receptor genes regulatory region PCR products and the pECFP-1 vector with the appropriate restriction endonucleases so that the 5' and 3' restriction endonuclease sites are ready for ligation. Ligation of the pECFP-1 vector DNA to the Di and D 5 dopamine receptor genes regulatory region PCR products DNA are accomplished using DNA ligase from the AdvantAgeTMPCR Cloning Kit (Clonetech Inc., Palo Alto, CA, USA) according to the manufacturer's recommendations.
- the ligated vector and insert construct is then used to transform TOP10F competent E. coli cells (Clonetech Inc., Palo Alto, CA, USA).
- the cells are plated on LB plus kanamycin containing agar and kanamycin resistant colonies are selected for further analysis.
- Kanamycin resistant clones are cultured in LB containing kanamycin medium and plasmid DNA is isolated using NucleoBond DNA Purification System (Clonetech Inc., Palo Alto, CA, USA) and the construct containing the Di and D 5 dopamine receptor genes regulatory region is analyzed by DNA sequencing to ensure construct correctness and integrity.
- Purified construct plasmid DNA containing the Di and D 5 dopamine receptor genes regulatory region is then transfected into the HEK293 cells utilizing calcium phosphate-mediated transfection utilizing the CalPhosTM Mammalian Transfection Kit (Clonetech Inc., Palo Alto, CA, USA).
- Transfected cell clones are selected using G418, isolated and propagated in DMEM (Life Technologies, Rockville, MD) containing 10% fetal bovine serum (Clonetech Inc., Palo Alto, CA, USA), penicillin/streptomycin solution (Life Technologies, Rockville, MD), L-glutamine (Life Technologies, Rockville, MD), non-essential amino acid (Life Technologies, Rockville, MD) and G418 (Life Technologies, Rockville, MD) at 37°C in a 5% carbon dioxide/95% air atmosphere.
- DMEM Life Technologies, Rockville, MD
- fetal bovine serum Clonetech Inc., Palo Alto, CA, USA
- penicillin/streptomycin solution Life Technologies, Rockville, MD
- L-glutamine Life Technologies, Rockville, MD
- non-essential amino acid Life Technologies, Rockville, MD
- G418 Life Technologies, Rockville, MD
- G418 resistant clones are characterized by Southern blotting to ensure that they contain the Di and D5 dopamine receptor genes promoter sequence; in addition activation of the Di and D 5 dopamine receptor genes regulatory region is analyzed using an appropriate stimulating agent.
- Cells expressing the Di and D 5 dopamine receptor genes regulatory region-ECFP at an appropriate level are then used in assays designed to evaluate compounds which can modulate the activity of the Di and D 5 dopamine receptor genes regulatory region as follows.
- the regulatory region activation analysis is performed by seeding the Di and D 5 dopamine receptor genes regulatory region-ECFP containing HEK293 cells at an appropriate density into black with clear bottom 96 well microtiter plates and allowed to grow overnight. The following day, the medium is removed and the test compound added in fresh growth medium.
- the cells are incubated for 16 hours at 37°C in a 5% carbon dioxide/95% air atmosphere followed by measurement of fluorescence (excitation at 433 (453) nm by detecting emission at 475(501) nm using a fluorometer (bioluminTM 960, Molecular Dynamics/Amersham Pharmacia Biotech, Piscataway, NJ).
- fluorescence excitation at 433 (453) nm by detecting emission at 475(501) nm using a fluorometer (bioluminTM 960, Molecular Dynamics/Amersham Pharmacia Biotech, Piscataway, NJ).
- Test compounds which stimulate fluorescence significantly above the levels of control untreated cells, after correction for variations in cell density, are considered candidate compounds for regulating skeletal muscle mass or function.
- the compounds of most interest are those which induce relatively higher levels of fluorescence.
- Example 6 Method of making human antibodies which activate the Di and D 5 dopamine receptors. Fully human monoclonal antibodies which activate the Di and D 5 dopamine receptors are produced by first generating recombinant Di and D5 dopamine receptor proteins as follows. The procedure from Example 1 is followed to obtain the Di and D 5 dopamine receptors PCR product. This Di and D 5 dopamine receptors PCR product is then cloned into the pHAT20 vector (Clonetech Inc., Palo Alto, CA, USA) by first cutting the Di and D 5 dopamine receptor gene PCR product and the pHAT20 vector with the appropriate restriction endonucleases so that the 5' and 3' restriction endonuclease sites are ready for ligation.
- Ligation of the pHAT20 vector DNA to the Di and D 5 dopamine receptor genes PCR product DNA is accomplished using DNA ligase from the AdvantAgeTMPCR Cloning Kit (Clonetech Inc., Palo Alto, CA, USA) according to the manufacturer's recommendations.
- the ligated vector/insert construct is then used to transform TOP10F' competent E. coli cells (Clonetech Inc., Palo Alto, CA, USA). Transformed cells are plated on LB plus ampicillin containing agar and ampicillin resistant colonies are selected for further analysis.
- Di and D 5 dopamine receptors -pHAT20 vector DNA is then used for additional PCR cloning by utilizing a 5' oligonucleotide containing the beginning of the HAT sequence and a unique restriction endonuclease site not present in the Di and D 5 dopamine receptors -pHAT20 construct and the 3' Di and D 5 dopamine receptors oligonucleotide utilized previously.
- the oligonucleotide primers are used to PCR amplify the HAT- Di and D 5 dopamine receptors fusion gene from the Di and D5 dopamine receptors -pHAT20 construct and the PCR product is purified as described above.
- the HAT- Di and D 5 dopamine receptors fusion gene PCR product is then utilized for cloning into the pBacPAK ⁇ vector using the BacPAK Baculovirus Expression System from Clonetech (Clonetech Inc., Palo Alto, CA, USA).
- the ligation of the HAT- Di and D 5 dopamine receptors fusion gene into the pBacPAK8 vector is essentially as described above.
- the Di and D 5 dopamine receptors /HAT-pBacPAK8 construct is then transfected into TOPIO'F competent E. coli cells, ampicillin resistant cells are selected and plasmid DNA is isolated and checked for construct integrity as described above.
- This construct is then cotransfected with linearized BacPAK ⁇ DNA into Sf21 insect host cells utilizing the CalPhosTM Mammalian Transfection Kit (Clonetech Inc., Palo Alto, CA, USA). The insect cells are then incubated for 2-3 days followed by harvest of virus from individual clear plaques.
- the virus is then amplified in Sf21 cells, the harvested virus titered, and the titered virus used for large scale infection of Sf21 cells utilizing BacPAK Insect Cell Media - all according to the manufacturers recommendations (Clonetech Inc., Palo Alto, CA, USA).
- Recombinant HAT-Di and D 5 dopamine receptor fusion proteins are then purified using the TALON® CellThru Purification Kit from Clonetech (Clonetech Inc., Palo Alto, CA, USA) using conditions recommended by the manufacturer. Briefly, infected Sf21 cells are harvested 48 hours after infection and sonicated in extraction/loading buffer. The cell lysate is then put through a TALON® CellThru column.
- the column is washed twice with extraction/loading buffer and the bound HAT- Di and D 5 dopamine receptor proteins are eluted with elution buffer.
- the eluted protein is analyzed by SDS-PAGE for integrity and protein concentration is quantitated using the Bio-Rad SDS-PAGE system and protein quantitation systems according to the manufacturer's recommendations (Bio-Rad Laboratories, Hercules, Ca).
- Purified HAT- Di and D 5 dopamine receptor fusion proteins are then used for immunizing XenoMouse animals (Abgenix Inc., Fremont, CA) for human monoclonal antibody production as follows.
- Serum is obtained from vaccinated animals and utilized in an antigen capture ELISA utilizing purified HAT- Di and D5 dopamine receptor fusion proteins to detect antibodies to the HAT- Di and D 5 dopamine receptor proteins by coating polystyrene ELISA plates (Corning Glass Works, Corning, NY) with HAT- Di and D5 dopamine receptor fusion proteins, blocked with PBS-1% BSA, washed and incubated at 37°C for 1 hour with a 1:50 dilution of the serum samples. After washing 5 times with PBS, the plates are incubated at 37°C for 1 hour with alkaline phosphatase-conjugated goat antibodies to human immunoglobulin G.
- Hybridomas are generated by fusion of splenic cells from the XenoMouse animals with nonsecreting myeloma cell line NSA-bcl 2 using a 4:1 ratio of spleen cells to NSA-bcl2 cells in the presence of 30% polyethylene glycol PEG1450.
- Fused cells are individually cloned by limiting dilution into 96 well plates and cultured in RPMI- 1640 medium containing 10% fetal bovine serum, nonessential amino acids, sodium pyruvate, L-glutamine, 100 u/ml penicillin-streptomycin and hypoxanthine-aminopterin-thymidine (all from Life Technologies, Rockville, MD).
- supernatants from the hypoxanthine- aminopterin-thymidine selected hybridomas were screened for human antibody production by ELISA as described previously.
- Hybridomas which produce human antibodies to the HAT- Di and D 5 dopamine receptors fusion protein are selected for large scale antibody production.
- Monoclonal antibodies are purified by Protein G-Sepharose chromatography. Briefly, the supernatant from cultured hybridoma clones is loaded onto a Protein G-Sepharose column (SIGMA, St. Louis, MO) in loading buffer, washed 3 times and the IgG is eluted with elution buffer. These antibodies are then used for screening to evaluate Di and D 5 dopamine receptors activation (agonism) potential. This is accomplished using the methodology as outlined in Example 3. Those human monoclonal antibodies which demonstrate agonist activity toward the Di and D 5 dopamine receptors are designated candidate compounds.
- SIGMA Protein G-Sepharose column
- Example 7 Determination of absolute force measurement of a muscle.
- the extensor digitorum longus (EDL) and soleus muscles are removed, tendon-to- tendon from the casted mouse leg.
- a silk suture is tied to each tendon of the isolated muscles and the muscles are placed into a plexiglass chamber filled with Ringer solution (137 mM sodium chloride, 24 mM sodium bicarbonate, 11 mM glucose, 5 mM potassium chloride, 1 mM magnesium sulfate, 1 mM sodium phosphate, 0.025 mM tubocurarine, all at pH 7.4 and oxygenated with 95% oxygen/5% carbon dioxide) constantly bubbled with 95% oxygen 5% carbon dioxide maintained at 25° C.
- Ringer solution 137 mM sodium chloride, 24 mM sodium bicarbonate, 11 mM glucose, 5 mM potassium chloride, 1 mM magnesium sulfate, 1 mM sodium phosphate, 0.025 mM tubocurarine, all
- Muscles are aligned horizontally between a servomotor lever arm (Model 305B-LR Cambridge Technology Inc., Watertown MA, USA) and the stainless steel hook of a force transducer (Model BG-50; Kulite Semiconductor Products Inc., Leonia, NJ, USA) and field stimulated by pulses transmitted between two platinum electrodes placed longitudinally on either side of the muscle.
- Square wave pulses (0.2 ms duration) generated by a personal computer with a Labview board (Model PCI-MIO 16E-4), Labview Inc., Austin, TX, USA) are amplified (Acurus power amplifier model A25, Dobbs Ferry, NY, USA) to increase titanic contraction.
- Stimulation voltage and muscle length (Lo) are adjusted to obtain maximum isometric twitch force. Maximum titanic force production (Po) is determined from the plateau of the frequency-force relationship.
- Example 8 Therapeutic treatment of skeletal muscle atrophy using a human antibody that is an agonist of the Di and D 5 dopamine receptor.
- 15 ml of an aqueous solution of pH 6 comprising an activating antibody of the Di and D 5 dopamine receptors are administered to the subject via intravenous injection.
- the solution comprises the following:
- the subject At the end of the treatment period, the subject exhibits measurable increases of muscle mass, strength and mobility of the arms and legs.
- Example 9 Prophylactic treatment of skeletal muscle atrophy using a human antibody that is an agonist of the Di and D 5 dopamine receptors.
- a human female subject weighing 55 kg is scheduled for hip joint replacement surgery in one month.
- the subject is treated to enhance skeletal muscle mass prior to and following surgery to ultimately reduce the level of skeletal muscle atrophy due to muscle disuse during post-surgery recovery.
- 18 ml of an aqueous solution of pH 6.0 comprising an activating antibody of the Di and D 5 dopamine receptors, are administered to the subject via intravenous injection.
- the solution comprises the following:
- the subject At the end of the treatment period, the subject exhibits measurable preservation of muscle mass, strength and mobility of the arms and legs as compared to the subject's expected status without antibody therapy.
- Example 10 Prophylactic treatment of skeletal muscle atrophy using a human antibody that is an agonist of the Di and D 5 dopamine receptors.
- the subject is treated to prevent atrophy of the skeletal muscle of the affected arm and shoulder due to disuse and limited use during fracture healing.
- 13 ml of pH 6.0 comprising the anti-Di and D 5 dopamine receptors activating antibody is administered to the subject via intravenous injection.
- the solution comprises the following: Component Concentration (mg/ml) dopamine receptor activating antibody 20 L-histidine HCI 0.47 L-histidine 0.3 ⁇ , ⁇ -trehalose dihydrate 20 Polysorbate 20 0.1 Bacteriostatic Sterile water qs to 1 ml
- the subject At the end of the treatment period, the subject exhibits measurable preservation of muscle mass, strength and mobility of the affected arm and shoulder and a reduced course of physical therapy as compared to the subject's expected status and follow-up treatment without antibody therapy.
- Example 11 Prophylactic treatment of skeletal muscle atrophy using Fenoldopam A human female subject weighing 60 kg is admitted to the hospital in a comatose state. The subject is treated by this method to prevent atrophy of the skeletal muscle of the entire body due to disuse in the comatose state. Specifically, once each day while in the coma, the subject is administered, via slow intravenous infusion, approximately 500 ml of an aqueous solution that is prepared by addition of 5 ml of the following stock solution to 500 ml of sterile saline:
- Component Concentration (mg/ml) Fenoldopam 12 S odium phosphate buffer, pH 7.4 140
- the subject exhibits measurable preservation of skeletal muscle mass and function, and reduced physical therapy needs during the coma and after regaining consciousness, as compared to the subject's status without drug therapy.
- Example 12 Therapeutic treatment of a patient with Duchenne Muscular dystrophy using Fenoldopam
- a male subject weighing 40 kg with an existing diagnosis of Duchenne's Muscular Dystrophy is treated with a sustained-release, depot formulation of Fenoldopam in order to improve or retain muscle strength and function over the progression of the disease.
- 3 ml of an aqueous solution of pH 6.0 comprising the following:
- the subject experiences either an improvement or an attenuation of the decline of muscle strength or muscle function in timed-function evaluations as compared to that exhibited during the natural progression of the disease.
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US10/299,642 US7067113B2 (en) | 2002-01-18 | 2002-11-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
CNA03826935XA CN101084436A (en) | 2002-01-18 | 2003-07-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
CA002532553A CA2532553A1 (en) | 2002-01-18 | 2003-07-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
AU2003256621A AU2003256621A1 (en) | 2002-01-18 | 2003-07-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
EP03818162A EP1646856A2 (en) | 2002-01-18 | 2003-07-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
PCT/US2003/022588 WO2005017484A2 (en) | 2002-01-18 | 2003-07-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
IL173162A IL173162A0 (en) | 2002-01-18 | 2006-01-16 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
IS8236A IS8236A (en) | 2002-01-18 | 2006-01-16 | Methods for Identifying Compounds that Control Mass or Muscle Activity Using Dopamine Receptors |
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US34962002P | 2002-01-18 | 2002-01-18 | |
US10/299,642 US7067113B2 (en) | 2002-01-18 | 2002-11-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
PCT/US2003/022588 WO2005017484A2 (en) | 2002-01-18 | 2003-07-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
Publications (2)
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WO2005017484A2 true WO2005017484A2 (en) | 2005-02-24 |
WO2005017484A3 WO2005017484A3 (en) | 2007-05-31 |
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PCT/US2003/022588 WO2005017484A2 (en) | 2002-01-18 | 2003-07-18 | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
Country Status (8)
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US (1) | US7067113B2 (en) |
EP (1) | EP1646856A2 (en) |
CN (1) | CN101084436A (en) |
AU (1) | AU2003256621A1 (en) |
CA (1) | CA2532553A1 (en) |
IL (1) | IL173162A0 (en) |
IS (1) | IS8236A (en) |
WO (1) | WO2005017484A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102276731A (en) * | 2011-08-16 | 2011-12-14 | 北京大学 | High-motion-activity polypeptide capable of inhibiting cocaine induction and application thereof |
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US7067113B2 (en) * | 2002-01-18 | 2006-06-27 | The Procter & Gamble Company | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
CN111676293B (en) * | 2020-05-15 | 2023-06-20 | 青海省畜牧兽医科学院 | SNP molecular marker related to Qinghai eight-eyebrow pig litter size trait and application thereof |
CN114480291A (en) * | 2021-12-30 | 2022-05-13 | 上海枢境生物科技有限公司 | Cell lines and methods for detecting biological activity of dopamine D4 target compound |
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US5215915A (en) * | 1991-04-16 | 1993-06-01 | Duke University | Cloned gene encoding rat d1b dopamine receptor |
US5389543A (en) * | 1990-05-14 | 1995-02-14 | Duke University | Cloned genes encoding the D1 dopamine receptor |
US5427942A (en) * | 1991-11-13 | 1995-06-27 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University | Human D5 dopamine recepltor gene and uses |
US7067113B2 (en) * | 2002-01-18 | 2006-06-27 | The Procter & Gamble Company | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
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WO1991000986A1 (en) | 1989-07-10 | 1991-01-24 | Tekelek Pty. Ltd. | Flow detection |
EP0538412A4 (en) * | 1990-07-06 | 1993-06-16 | Us Health | A cdna encoding the rat d 1? dopamine receptor linked to adenylyl cyclase activation and expression product |
EP0538395A4 (en) * | 1990-07-10 | 1993-06-16 | Synaptic Pharmaceutical Corporation | Nucleic acid sequences encoding a human dopamine d 1? receptor |
CA2024096A1 (en) | 1990-08-27 | 1992-02-28 | Philip Seeman | Gene clone for the human dopamine d1 receptor |
WO1994005695A1 (en) | 1992-09-10 | 1994-03-17 | New York University | Polypeptides of g-coupled receptor proteins, and compositions and methods thereof |
EP0906339A2 (en) | 1996-03-27 | 1999-04-07 | NG, Gordon, Y., K. | Receptor and transporter antagonists |
-
2002
- 2002-11-18 US US10/299,642 patent/US7067113B2/en not_active Expired - Fee Related
-
2003
- 2003-07-18 EP EP03818162A patent/EP1646856A2/en not_active Withdrawn
- 2003-07-18 CN CNA03826935XA patent/CN101084436A/en active Pending
- 2003-07-18 AU AU2003256621A patent/AU2003256621A1/en not_active Abandoned
- 2003-07-18 WO PCT/US2003/022588 patent/WO2005017484A2/en active Application Filing
- 2003-07-18 CA CA002532553A patent/CA2532553A1/en not_active Abandoned
-
2006
- 2006-01-16 IS IS8236A patent/IS8236A/en unknown
- 2006-01-16 IL IL173162A patent/IL173162A0/en unknown
Patent Citations (4)
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US5389543A (en) * | 1990-05-14 | 1995-02-14 | Duke University | Cloned genes encoding the D1 dopamine receptor |
US5215915A (en) * | 1991-04-16 | 1993-06-01 | Duke University | Cloned gene encoding rat d1b dopamine receptor |
US5427942A (en) * | 1991-11-13 | 1995-06-27 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University | Human D5 dopamine recepltor gene and uses |
US7067113B2 (en) * | 2002-01-18 | 2006-06-27 | The Procter & Gamble Company | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
Non-Patent Citations (2)
Title |
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BARTON A.C. ET AL.: 'Agonist-Induced Desensitization of D1-Dopamine Receptors Linked to Adenylyl Cyclase Activity in Cultured NS20Y Neuroblastoma Cells' MOLECUALR PHARMACOLOGY vol. 38, 1990, pages 531 - 541, XP008082005 * |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102276731A (en) * | 2011-08-16 | 2011-12-14 | 北京大学 | High-motion-activity polypeptide capable of inhibiting cocaine induction and application thereof |
Also Published As
Publication number | Publication date |
---|---|
US7067113B2 (en) | 2006-06-27 |
CA2532553A1 (en) | 2005-02-24 |
US20030170741A1 (en) | 2003-09-11 |
EP1646856A2 (en) | 2006-04-19 |
CN101084436A (en) | 2007-12-05 |
AU2003256621A1 (en) | 2005-03-07 |
WO2005017484A3 (en) | 2007-05-31 |
IS8236A (en) | 2006-01-16 |
IL173162A0 (en) | 2006-06-11 |
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