CA2024096A1 - Gene clone for the human dopamine d1 receptor - Google Patents
Gene clone for the human dopamine d1 receptorInfo
- Publication number
- CA2024096A1 CA2024096A1 CA 2024096 CA2024096A CA2024096A1 CA 2024096 A1 CA2024096 A1 CA 2024096A1 CA 2024096 CA2024096 CA 2024096 CA 2024096 A CA2024096 A CA 2024096A CA 2024096 A1 CA2024096 A1 CA 2024096A1
- Authority
- CA
- Canada
- Prior art keywords
- gene
- receptor
- dopamine
- protein
- gene clone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Form 24 [GENE CLONE FOR THE HUMAN DOPAMINE D1 RECEPTOR]
ABSTRACT
Biological receptors for dopamine are presently classified into two types, termed D1 and D2. The D1 receptor is defined as the protein on which dopamine acts to stimulate the enzyme adenylate cyclase; this latter enzyme is inhibited when dopamine acts on D2. D1 and D2 are targets of drug therapy in psychomotor illnesses such as Parkinson's disease, schizophrenia, and drug addiction.
We here report and patent the D1 receptor gene and its sequence of nucleotide bases. The D1 protein which is made by this gene has 446 amino acids. The D1 gene contains regions {susceptible to restriction enzymes, particularly EcoR1}which differ between humans, making this D1 gene helpful for future studies attempting to link or associate the gene with various human diseases.
ABSTRACT
Biological receptors for dopamine are presently classified into two types, termed D1 and D2. The D1 receptor is defined as the protein on which dopamine acts to stimulate the enzyme adenylate cyclase; this latter enzyme is inhibited when dopamine acts on D2. D1 and D2 are targets of drug therapy in psychomotor illnesses such as Parkinson's disease, schizophrenia, and drug addiction.
We here report and patent the D1 receptor gene and its sequence of nucleotide bases. The D1 protein which is made by this gene has 446 amino acids. The D1 gene contains regions {susceptible to restriction enzymes, particularly EcoR1}which differ between humans, making this D1 gene helpful for future studies attempting to link or associate the gene with various human diseases.
Description
SPh`CIFlCA~lON
This invenlion is the full-length DNA sequence for the gene for the human dopamine D1 receptor protein.
In order to study biological processes in various body tissues in which Lhe chemical dopamine is involved, it is desirable to have pure systems s~r biological cells which contain the pure genes for dopamine receptors. Isolating the pure dopamine D1 receptor protein has entailed many difficulties and has not hitherto heen successful.
Our isolation of the dopamine D1 gene enables biologists to study precisely how lhis reccptor protein operates in a biological c~ll and how the D1 protein interacts with other componenls of ~he cell.
In order to obtain the full-length DNA sequence for D1, we uscd our previously reported partial clone for rat D1 of 450 nucleotide base pairs (bp), termèd G36 (Reference 12 at end of this Specificalion). We used G36 to probe a Lambda EMBL 3 SP6-T7 human genomic library (Clontech company), and isolated three positive clones with inserts of about 14 kilobases (kb).
Sequence and restriction map analysis indictaed that one of these clones (termed RS1) contained sequence similar to the rat complementary clone G36. Sequencing of overlapping restriction fragments of RS1 (Fig. la) revealed a long open reading frame of 1,476 nucleotides (Fig. lb) coding a 446-amino acid protein.
The proof that this DNA sequence (in Fig. lb) and the corresponding protein sequence (Fig. 1c) is the D1 receptor is provided in Figs. 2 and 3.
~ The Figs. 1, 2, 3 and 4 and the associated descriptive text are to be published in Nature magazine on September 6th, 1990, by Macmillan Press, London, England.}
Our c]aim in this patent is to the DNA sequence sh~wn in Fi~. lb . . . .
:, -, . , , . :
; . . . . . . . .
This invenlion is the full-length DNA sequence for the gene for the human dopamine D1 receptor protein.
In order to study biological processes in various body tissues in which Lhe chemical dopamine is involved, it is desirable to have pure systems s~r biological cells which contain the pure genes for dopamine receptors. Isolating the pure dopamine D1 receptor protein has entailed many difficulties and has not hitherto heen successful.
Our isolation of the dopamine D1 gene enables biologists to study precisely how lhis reccptor protein operates in a biological c~ll and how the D1 protein interacts with other componenls of ~he cell.
In order to obtain the full-length DNA sequence for D1, we uscd our previously reported partial clone for rat D1 of 450 nucleotide base pairs (bp), termèd G36 (Reference 12 at end of this Specificalion). We used G36 to probe a Lambda EMBL 3 SP6-T7 human genomic library (Clontech company), and isolated three positive clones with inserts of about 14 kilobases (kb).
Sequence and restriction map analysis indictaed that one of these clones (termed RS1) contained sequence similar to the rat complementary clone G36. Sequencing of overlapping restriction fragments of RS1 (Fig. la) revealed a long open reading frame of 1,476 nucleotides (Fig. lb) coding a 446-amino acid protein.
The proof that this DNA sequence (in Fig. lb) and the corresponding protein sequence (Fig. 1c) is the D1 receptor is provided in Figs. 2 and 3.
~ The Figs. 1, 2, 3 and 4 and the associated descriptive text are to be published in Nature magazine on September 6th, 1990, by Macmillan Press, London, England.}
Our c]aim in this patent is to the DNA sequence sh~wn in Fi~. lb . . . .
:, -, . , , . :
; . . . . . . . .
Claims
Our claim in this patent is to the DNA sequence shown in Fig, 1b
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2024096 CA2024096A1 (en) | 1990-08-27 | 1990-08-27 | Gene clone for the human dopamine d1 receptor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2024096 CA2024096A1 (en) | 1990-08-27 | 1990-08-27 | Gene clone for the human dopamine d1 receptor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2024096A1 true CA2024096A1 (en) | 1992-02-28 |
Family
ID=4145829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2024096 Abandoned CA2024096A1 (en) | 1990-08-27 | 1990-08-27 | Gene clone for the human dopamine d1 receptor |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2024096A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7067113B2 (en) | 2002-01-18 | 2006-06-27 | The Procter & Gamble Company | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
-
1990
- 1990-08-27 CA CA 2024096 patent/CA2024096A1/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7067113B2 (en) | 2002-01-18 | 2006-06-27 | The Procter & Gamble Company | Methods for identifying compounds for regulating muscle mass or function using dopamine receptors |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |