WO2005015155A3 - Methodes de comparaison des debits relatifs d'au moins deux molecules biologiques in vivo au moyen d'un seul protocole - Google Patents

Methodes de comparaison des debits relatifs d'au moins deux molecules biologiques in vivo au moyen d'un seul protocole Download PDF

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Publication number
WO2005015155A3
WO2005015155A3 PCT/US2004/020319 US2004020319W WO2005015155A3 WO 2005015155 A3 WO2005015155 A3 WO 2005015155A3 US 2004020319 W US2004020319 W US 2004020319W WO 2005015155 A3 WO2005015155 A3 WO 2005015155A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological molecules
flux rates
vivo
methods
comparing relative
Prior art date
Application number
PCT/US2004/020319
Other languages
English (en)
Other versions
WO2005015155A2 (fr
Inventor
Marc K Hellerstein
Original Assignee
Univ California
Marc K Hellerstein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Marc K Hellerstein filed Critical Univ California
Publication of WO2005015155A2 publication Critical patent/WO2005015155A2/fr
Publication of WO2005015155A3 publication Critical patent/WO2005015155A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des techniques permettant de mesurer et de comparer les débits moléculaires relatifs de différentes molécules biologiques. Ces techniques consistent à administrer de l'eau marquée par des isotopes à un ou plusieurs tissus ou sujets et à comparer les débits moléculaires d'au moins deux molécules biologiques, notamment des molécules biologiques dans différentes classes chimiques. Ces méthodes sont notamment utiles pour le diagnostic, le pronostic ou le monitorage d'une maladie, d'un trouble ou d'une affection, le criblage in vivo à haut rendement d'entités chimiques et les facteurs biologiques pour les effets toxiques.
PCT/US2004/020319 2003-07-03 2004-06-23 Methodes de comparaison des debits relatifs d'au moins deux molecules biologiques in vivo au moyen d'un seul protocole WO2005015155A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48462603P 2003-07-03 2003-07-03
US60/484,626 2003-07-03

Publications (2)

Publication Number Publication Date
WO2005015155A2 WO2005015155A2 (fr) 2005-02-17
WO2005015155A3 true WO2005015155A3 (fr) 2005-08-04

Family

ID=34102659

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2004/019626 WO2005009597A2 (fr) 2003-07-03 2004-06-17 Methodes de comparaison des debits relatifs de deux ou de plusieurs molecules biologiques in vivo a l'aide d'un seul protocole
PCT/US2004/020319 WO2005015155A2 (fr) 2003-07-03 2004-06-23 Methodes de comparaison des debits relatifs d'au moins deux molecules biologiques in vivo au moyen d'un seul protocole

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2004/019626 WO2005009597A2 (fr) 2003-07-03 2004-06-17 Methodes de comparaison des debits relatifs de deux ou de plusieurs molecules biologiques in vivo a l'aide d'un seul protocole

Country Status (2)

Country Link
TW (1) TW200513649A (fr)
WO (2) WO2005009597A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5910403A (en) 1997-05-15 1999-06-08 The Regents Of University Of California Methods for measuring cellular proliferation and destruction rates in vitro and in vivo
WO2003061479A1 (fr) 2001-10-24 2003-07-31 The Regents Of The University Of California Mesure du taux de synthese de proteines chez des humains et dans des systemes experimentaux au moyen d'eau marquee d'une signature isotopique
JP4804753B2 (ja) 2002-07-30 2011-11-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 質量分析法を使用した、プロテオームまたはオルガネオームの分子フラックス速度の自動化大規模測定
WO2004025270A2 (fr) 2002-09-13 2004-03-25 The Regents Of The University Of California Methodes permettant de mesurer les taux de transport inverse du cholesterol in vivo utilises comme indice d'anti-atherogenicite
US7262020B2 (en) 2003-07-03 2007-08-28 The Regents Of The University Of California Methods for comparing relative flux rates of two or more biological molecules in vivo through a single protocol
US20050202406A1 (en) 2003-11-25 2005-09-15 The Regents Of The University Of California Method for high-throughput screening of compounds and combinations of compounds for discovery and quantification of actions, particularly unanticipated therapeutic or toxic actions, in biological systems
TW200538738A (en) 2004-02-20 2005-12-01 Univ California Molecular flux rates through critical pathways measured by stable isotope labeling in vivo, as biomarkers of drug action and disease activity
US10386371B2 (en) 2011-09-08 2019-08-20 The Regents Of The University Of California Metabolic flux measurement, imaging and microscopy
US20140353486A1 (en) 2011-12-07 2014-12-04 Glaxosmithkline Llc Methods for determining total body skeletal muscle mass
US9134319B2 (en) 2013-03-15 2015-09-15 The Regents Of The University Of California Method for replacing biomarkers of protein kinetics from tissue samples by biomarkers of protein kinetics from body fluids after isotopic labeling in vivo

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HELLERSTEIN M. ET AL.: "Mass isoopomer distribution anaylsis at eight years", AMERICAN JOURNAL OF PHYSIOLOGY, vol. 276, 1999, pages E1146 - 1170, XP002978087 *
HSIEH E. ET AL.: "Dynamics of keratinocytes in vivo using 2H2O labeling", J. INVEST. DERMATOL., vol. 123, no. 3, 2004, pages 530 - 536 *
NEESE R. ET AL.: "Measurement in vivo of proliferation rates of slow turnover cells by 2H2O labeling of the deoxyribose moiery of DNA", PNAS, vol. 99, no. 24, 26 November 2002 (2002-11-26), pages 15345 - 15350 *

Also Published As

Publication number Publication date
WO2005009597A2 (fr) 2005-02-03
WO2005015155A2 (fr) 2005-02-17
TW200513649A (en) 2005-04-16

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