WO2005014796A3 - Methods and compositions for seamless cloning of nucleic acid molecules - Google Patents

Methods and compositions for seamless cloning of nucleic acid molecules Download PDF

Info

Publication number
WO2005014796A3
WO2005014796A3 PCT/US2004/025831 US2004025831W WO2005014796A3 WO 2005014796 A3 WO2005014796 A3 WO 2005014796A3 US 2004025831 W US2004025831 W US 2004025831W WO 2005014796 A3 WO2005014796 A3 WO 2005014796A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
present
methods
cloning
Prior art date
Application number
PCT/US2004/025831
Other languages
French (fr)
Other versions
WO2005014796A2 (en
Inventor
Jonathan D Chesnut
Knut R Madden
Miroslav Dudas
Louis Leong
Adam N Harris
Original Assignee
Invitrogen Corp
Jonathan D Chesnut
Knut R Madden
Miroslav Dudas
Louis Leong
Adam N Harris
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp, Jonathan D Chesnut, Knut R Madden, Miroslav Dudas, Louis Leong, Adam N Harris filed Critical Invitrogen Corp
Priority to EP04786487A priority Critical patent/EP1687323A4/en
Publication of WO2005014796A2 publication Critical patent/WO2005014796A2/en
Publication of WO2005014796A3 publication Critical patent/WO2005014796A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y599/00—Other isomerases (5.99)
    • C12Y599/01—Other isomerases (5.99.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.
PCT/US2004/025831 2003-08-08 2004-08-09 Methods and compositions for seamless cloning of nucleic acid molecules WO2005014796A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04786487A EP1687323A4 (en) 2003-08-08 2004-08-09 Methods and compositions for seamless cloning of nucleic acid molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49332203P 2003-08-08 2003-08-08
US60/493,322 2003-08-08

Publications (2)

Publication Number Publication Date
WO2005014796A2 WO2005014796A2 (en) 2005-02-17
WO2005014796A3 true WO2005014796A3 (en) 2005-06-23

Family

ID=34135228

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/025831 WO2005014796A2 (en) 2003-08-08 2004-08-09 Methods and compositions for seamless cloning of nucleic acid molecules

Country Status (3)

Country Link
US (6) US20050069929A1 (en)
EP (2) EP2484687A3 (en)
WO (1) WO2005014796A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7304130B2 (en) 1995-06-07 2007-12-04 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US7393632B2 (en) 1999-12-10 2008-07-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US8883988B2 (en) 1999-03-02 2014-11-11 Life Technologies Corporation Compositions for use in recombinational cloning of nucleic acids
US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US9534252B2 (en) 2003-12-01 2017-01-03 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
CN101125873A (en) * 1997-10-24 2008-02-20 茵维特罗根公司 Recombinational cloning using nucleic acids having recombination sites
WO1999021977A1 (en) * 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
US20040219516A1 (en) * 2002-07-18 2004-11-04 Invitrogen Corporation Viral vectors containing recombination sites
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
US20050069929A1 (en) 2003-08-08 2005-03-31 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US20060166334A1 (en) * 2004-12-21 2006-07-27 Genecopoeia, Inc. Method and compositions for rapidly modifying clones
GB2421948A (en) * 2004-12-30 2006-07-12 Ist Superiore Sanita Retrotransposon inhibition to treat cancer
US7582475B2 (en) 2005-01-07 2009-09-01 University Of Georgia Research Foundation, Inc. Vectors and methods for high throughput co-expression
US20060154370A1 (en) * 2005-01-11 2006-07-13 Yuzhi Chen Efficient gene suppression using a transfer RNA promoter in herpes virus vectors to deliver small interference RNAs
US8080393B2 (en) * 2005-04-12 2011-12-20 Olink Ab Methods for production of oligonucleotides
CN101314775B (en) 2007-05-31 2013-02-06 厦门大学 RNA interference targets for AIDS treatment
US8323930B2 (en) * 2007-07-28 2012-12-04 Dna Twopointo, Inc. Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase
WO2009025209A1 (en) * 2007-08-21 2009-02-26 National University Corporation Hokkaido University Method for preparation of recombinant dna
CN101348799B (en) * 2008-07-18 2011-01-26 华中科技大学同济医学院附属同济医院 Construction method and pharmaceutical application of severe hepatitis related gene hfgl2 microRNA adenovirus expression plasmid
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
EP2427577A4 (en) 2009-05-04 2013-10-23 Hutchinson Fred Cancer Res PSEUDOTYPED COCAL VIRUS ENVELOPE RETROVIRAL VECTORS (VESICULOVIRUS)
ES2574055T3 (en) 2009-11-25 2016-06-14 Gen9, Inc. Methods and apparatus for error reduction in chip-based DNA
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
EP3360963B1 (en) 2010-11-12 2019-11-06 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP4039363A1 (en) 2010-11-12 2022-08-10 Gen9, Inc. Protein arrays and methods of using and making the same
IL280334B2 (en) 2011-08-26 2023-09-01 Gen9 Inc Compositions and methods for high fidelity assembly of nucleic acids
AU2013251701A1 (en) 2012-04-24 2014-10-30 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
CA2877823A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
LT2970361T (en) 2013-03-15 2023-05-10 Gen9, Inc. Compositions and methods for multiplex nucleic acids synthesis
US9206433B2 (en) 2013-05-01 2015-12-08 Dna Twopointo, Inc. Methods, compositions and kits for a one-step DNA cloning system
US10253321B2 (en) 2013-05-01 2019-04-09 Dna2.0, Inc. Methods, compositions and kits for a one-step DNA cloning system
CN106544325B (en) * 2015-09-23 2018-09-07 中国科学院武汉物理与数学研究所 A kind of recombinant baculovirus and its application
GB2628173A (en) 2023-03-17 2024-09-18 Thermo Fisher Scient Geneart Gmbh Methods of producing modified nucleic acid sequences for eliminating adverse splicing events
WO2024254373A2 (en) * 2023-06-09 2024-12-12 Howard Hughes Medical Institute Vectors and methods for efficient cloning and homology directed repair

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020007051A1 (en) * 1999-12-10 2002-01-17 David Cheo Use of multiple recombination sites with unique specificity in recombinational cloning
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US20030139363A1 (en) * 2001-07-23 2003-07-24 Kay Mark A. Methods and compositions for RNAi mediated inhibition of viral gene expression in mammals

Family Cites Families (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4216245A (en) 1978-07-25 1980-08-05 Miles Laboratories, Inc. Method of making printed reagent test devices
US4293652A (en) 1979-05-25 1981-10-06 Cetus Corporation Method for synthesizing DNA sequentially
US4372745A (en) 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4670572A (en) 1981-07-01 1987-06-02 Eastman Kodak Company Phenolic fluorescent labels
US4525048A (en) 1983-03-21 1985-06-25 W. Haking Enterprises, Limited Automatic slot positioning device for camera take-up spool
US4517338A (en) 1983-06-20 1985-05-14 Chiron Corporation Multiple reactor system and method for polynucleotide synthesis
US4859587A (en) 1984-06-04 1989-08-22 Institut Merieux Recombinant herpes simplex viruses, vaccines and methods
US4736866B1 (en) 1984-06-22 1988-04-12 Transgenic non-human mammals
DK365785A (en) 1984-09-17 1986-03-18 Hoffmann La Roche metal complex
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5422096A (en) 1985-05-08 1995-06-06 The General Hospital Corporation Hydroxy-aryl metal chelates for diagnostic NMR imaging
US6121043A (en) 1985-09-06 2000-09-19 Syntro Corporation Recombinant herpesvirus of turkeys comprising a foreign DNA inserted into a non-essential region of the herpesvirus of turkeys genome
US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
US5125748A (en) 1986-03-26 1992-06-30 Beckman Instruments, Inc. Optical detection module for use in an automated laboratory work station
US5104621A (en) 1986-03-26 1992-04-14 Beckman Instruments, Inc. Automated multi-purpose analytical chemistry processing center and laboratory work station
US5206568A (en) 1986-03-26 1993-04-27 Beckman Instruments, Inc. Coordinated control of stepper motors
US5139744A (en) 1986-03-26 1992-08-18 Beckman Instruments, Inc. Automated laboratory work station having module identification means
US5262176A (en) 1986-07-03 1993-11-16 Advanced Magnetics, Inc. Synthesis of polysaccharide covered superparamagnetic oxide colloids
US5221623A (en) 1986-07-22 1993-06-22 Boyce Thompson Institute For Plant Research, Inc. Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5672344A (en) 1987-12-30 1997-09-30 The Regents Of The University Of Michigan Viral-mediated gene transfer system
US5656207A (en) 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
US5683888A (en) 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5242681A (en) 1990-03-12 1993-09-07 Research Corporation Technologies, Inc. Lipophilic contrast agents for diagnostic image analysis
US5789573A (en) 1990-08-14 1998-08-04 Isis Pharmaceuticals, Inc. Antisense inhibition of ICAM-1, E-selectin, and CMV IE1/IE2
US5283179A (en) 1990-09-10 1994-02-01 Promega Corporation Luciferase assay method
US5348788A (en) 1991-01-30 1994-09-20 Interpore Orthopaedics, Inc. Mesh sheet with microscopic projections and holes
ZA929351B (en) 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
EP0552108B1 (en) 1992-01-17 1999-11-10 Lakowicz, Joseph R. Energy transfer phase-modulation fluoro-immunoassay
US5355215A (en) 1992-09-30 1994-10-11 Environmental Research Institute Of Michigan Method and apparatus for quantitative fluorescence measurements
US5334575A (en) 1992-12-17 1994-08-02 Eastman Kodak Company Dye-containing beads for laser-induced thermal dye transfer
WO1994024296A2 (en) 1993-04-19 1994-10-27 University Of Saskatchewan Recombinant bovine herpesvirus type 1 vaccines
US5350564A (en) 1993-06-28 1994-09-27 Baxter Diagnostics Inc. Automated chemical analyzer with apparatus and method for conveying and temporary storage of sample tubes
EP0641035B1 (en) 1993-08-24 2000-11-15 Matsushita Electric Industrial Co., Ltd. A laminated antenna duplexer and a dielectric filter
US5624803A (en) * 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
DK0724885T3 (en) 1993-10-21 2002-05-21 Hisamitsu Pharmaceutical Co Pernasal composition and pernasal preparation containing this
US5807746A (en) 1994-06-13 1998-09-15 Vanderbilt University Method for importing biologically active molecules into cells
US5622821A (en) 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
JP3466765B2 (en) 1994-07-27 2003-11-17 キッコーマン株式会社 Biotinylated firefly luciferase, biotinylated firefly luciferase gene, novel recombinant DNA, method for producing biotinylated firefly luciferase and bioluminescence analysis method
GB9415369D0 (en) 1994-07-29 1994-09-21 Lynxvale Ltd Mutant virus
US5601980A (en) 1994-09-23 1997-02-11 Hewlett-Packard Company Manufacturing method and apparatus for biological probe arrays using vision-assisted micropipetting
JP3031178B2 (en) 1994-09-28 2000-04-10 株式会社村田製作所 Composite high frequency components
US5777079A (en) 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
AU704660B2 (en) * 1994-11-28 1999-04-29 E.I. Du Pont De Nemours And Company Compound microsatellite primers for the detection of genetic polymorphisms
US5589351A (en) 1994-12-06 1996-12-31 Nps Pharmaceuticals, Inc. Fluorescence detection apparatus
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
KR100298617B1 (en) 1995-02-03 2002-07-31 가부시키가이샤 덴소 Fuel supply system and its supporting unit
US5741657A (en) 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use
DK0937098T3 (en) 1995-06-07 2002-12-02 Invitrogen Corp Recombinational cloning using genetically engineered recombination sites
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5877021A (en) 1995-07-07 1999-03-02 Ribozyme Pharmaceuticals, Inc. B7-1 targeted ribozymes
US5851826A (en) 1995-07-26 1998-12-22 Children's Medical Center Corporation Helper virus-free herpesvirus vector packaging system
US6261797B1 (en) * 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6228646B1 (en) 1996-03-07 2001-05-08 The Regents Of The University Of California Helper-free, totally defective adenovirus for gene therapy
US5721435A (en) 1996-04-09 1998-02-24 Hewlett Packard Company Methods and apparatus for measuring optical properties of biological and chemical substances
FR2748569B1 (en) 1996-05-07 1998-08-07 Biocom Sa METHOD AND PLANT FOR SEPARATING MAGNETIC PARTICLES IN A FLUID FOR BIOLOGICAL ANALYSIS, AND APPLICATION OF SAID METHOD
US5702926A (en) * 1996-08-22 1997-12-30 Becton, Dickinson And Company Nicking of DNA using boronated nucleotides
GB9618477D0 (en) 1996-09-04 1996-10-16 Univ Leeds Gene therapy
US5735862A (en) 1996-09-23 1998-04-07 Jennings; Erwin Reeves Semi-automatic suturing and sewing device
US5800999A (en) 1996-12-16 1998-09-01 Tropix, Inc. Dioxetane-precursor-labeled probes and detection assays employing the same
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US5917012A (en) 1997-04-03 1999-06-29 Wako Pure Chemical Industries, Ltd. Peptide derivatives
US6410311B1 (en) 1997-05-09 2002-06-25 Schering-Plough Veterinary Corporation Recombinant feline herpesvirus comprising a foreign DNA inserted into a region corresponding to a 3.0 kb EcoRI-SalI fragment of a feline herpesvirus genome
ATE283353T1 (en) 1997-06-12 2004-12-15 Sloan Kettering Inst Cancer COVALENT BINDING OF DNA TO RNA STRANDS CATALYSED BY VACCINIA TOPOISOMERASE
US6054271A (en) 1997-10-21 2000-04-25 The Regents Of The University Of California Methods of using synthetic molecules and target sequences
US5932474A (en) 1997-10-21 1999-08-03 The Regents Of The University Of California Target sequences for synthetic molecules
US6008378A (en) 1997-10-21 1999-12-28 The Regents Of The University Of California Synthetic molecules that specifically react with target sequences
WO1999021977A1 (en) 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
CA2759882A1 (en) 1997-12-05 1999-06-17 Europaisches Laboratorium Fur Molekularbiologie Novel dna cloning method
DE69938128D1 (en) * 1998-03-28 2008-03-27 Univ Utah Res Found TARGETED ANTISENSE LIBRARIES
US6197584B1 (en) 1998-05-01 2001-03-06 Isis Pharmaceuticals, Inc. Antisense modulation of CD40 expression
US6088214A (en) 1998-06-01 2000-07-11 Motorola, Inc. Voltage variable capacitor array and method of manufacture thereof
GB9816761D0 (en) 1998-07-31 1998-09-30 Phogen Limited Herpesvirus preparations and their uses
WO2000052141A1 (en) * 1999-03-02 2000-09-08 Invitrogen Corporation Cells resistant to toxic genes and uses thereof
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US6355412B1 (en) 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
JP2001136045A (en) 1999-08-23 2001-05-18 Murata Mfg Co Ltd Layered composite electronic component
DE19941186A1 (en) 1999-08-30 2001-03-01 Peter Droege Sequence-specific DNA recombination in eukaryotic cells
US20020025561A1 (en) * 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
GB0016747D0 (en) 2000-07-08 2000-08-30 Johnson Matthey Plc Ink
WO2002046372A1 (en) * 2000-12-08 2002-06-13 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20070196838A1 (en) 2000-12-08 2007-08-23 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
CA2445049A1 (en) 2001-05-04 2002-11-14 Cornell Research Foundation, Inc. Rapidly cleavable sumo fusion protein expression system for difficult to express proteins
WO2003046173A1 (en) * 2001-11-28 2003-06-05 Center For Advanced Science And Technology Incubation, Ltd. siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE-KNOCKDOWN CELLS AND THE LIKE USING THE SAME
AU2003257109A1 (en) 2002-08-05 2004-02-23 Invitrogen Corporation Compositions and methods for molecular biology
WO2004108897A2 (en) * 2003-06-02 2004-12-16 Cytokinetics, Inc. Sirna libraries
US20050069929A1 (en) 2003-08-08 2005-03-31 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US12239299B2 (en) 2010-02-25 2025-03-04 Pave, Llc Full core biopsy device
US9516099B1 (en) 2014-09-23 2016-12-06 Amazon Technologies, Inc. Controlling access to digital items based on weather conditions
KR102423818B1 (en) 2015-12-18 2022-07-21 삼성전자주식회사 A electrostatic chuck assembly, a semiconductor manufacturing apparatus having the same, and a temperature mesuring method for electrostatic chuck

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020007051A1 (en) * 1999-12-10 2002-01-17 David Cheo Use of multiple recombination sites with unique specificity in recombinational cloning
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US20030139363A1 (en) * 2001-07-23 2003-07-24 Kay Mark A. Methods and compositions for RNAi mediated inhibition of viral gene expression in mammals

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7304130B2 (en) 1995-06-07 2007-12-04 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US8883988B2 (en) 1999-03-02 2014-11-11 Life Technologies Corporation Compositions for use in recombinational cloning of nucleic acids
US7393632B2 (en) 1999-12-10 2008-07-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US9309520B2 (en) 2000-08-21 2016-04-12 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US9534252B2 (en) 2003-12-01 2017-01-03 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same

Also Published As

Publication number Publication date
EP2484687A2 (en) 2012-08-08
EP2484687A3 (en) 2012-11-14
EP1687323A2 (en) 2006-08-09
WO2005014796A2 (en) 2005-02-17
EP1687323A4 (en) 2009-08-12
US20150152460A1 (en) 2015-06-04
US20120149069A1 (en) 2012-06-14
US20130157263A1 (en) 2013-06-20
US20050069929A1 (en) 2005-03-31
US20110046201A1 (en) 2011-02-24
US8338091B2 (en) 2012-12-25
US20140170710A1 (en) 2014-06-19

Similar Documents

Publication Publication Date Title
WO2005014796A3 (en) Methods and compositions for seamless cloning of nucleic acid molecules
WO2002086144A3 (en) Compositions and methods for recombinational cloning of nucleic acid molecules
WO2006073839A3 (en) Acid fungal proteases
WO2004099387A3 (en) siRNA INDUCED SYSTEMIC GENE SILENCING IN MAMMALIAN SYSTEMS
WO2004058990A3 (en) Genetic polymorphisms associated with stenosis, methods of detection and uses thereof
WO2004067779A3 (en) Genetic polymorphisms associated with rheumatoid arthritis, methods of detection and uses thereof
WO2006113246A3 (en) Small activating rna molecules and methods of use
WO2005111241A8 (en) Genetic polymorphisms associated with liver fibrosis methods of detection and uses thereof
WO2004057017A3 (en) Detection of small nucleic acids
WO2005069762A8 (en) Bacillus licheniformis chromosome
DK1419255T3 (en) A new group of alpha-amylases as well as a method for identifying and recovering new alpha-amylases.
WO2004007718A3 (en) Rna-interference by single-stranded rna molecules
WO2004081186A3 (en) Single nucleotide polymorphisms associated with stenosis, methods of detection and uses thereof
WO2003025117A3 (en) Cloning and sequencing of pyruvate decarboxylase (pdc) genes from bacteria and uses therefor
WO2007136835A3 (en) Methods and cells for creating functional diversity and uses thereof
WO2002014485A3 (en) Kallikrein gene
Watanabe et al. tRNA modification and genetic code variations in animal mitochondria
WO2005033094A3 (en) Methods for the production of 3-methylamino-1-(thiene-2-yl)-propane-1-ol
WO2005054438A3 (en) Nucleic acid molecules containing recombination sites and methods of using the same
WO2004058976A3 (en) Borrelidin-producing polyketide synthase and its use
WO2004081187A3 (en) Genetic polymorphisms associated with myocardial infarction, methods of detection and uses thereof
WO2003104470A3 (en) Retrons for gene targeting
WO2003040681A3 (en) Genes coding for metabolic pathway proteins
WO2005110067A3 (en) Method and compositions for rna interference
WO2004083403A3 (en) Genetic polymorphisms associated with rheumatoid arthritis, methods of detection and uses thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2004786487

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2004786487

Country of ref document: EP