WO2005014794A3 - Generation of low phytate plants by molecular disruption of inositol polyphosphate kinases - Google Patents

Generation of low phytate plants by molecular disruption of inositol polyphosphate kinases Download PDF

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Publication number
WO2005014794A3
WO2005014794A3 PCT/US2004/025756 US2004025756W WO2005014794A3 WO 2005014794 A3 WO2005014794 A3 WO 2005014794A3 US 2004025756 W US2004025756 W US 2004025756W WO 2005014794 A3 WO2005014794 A3 WO 2005014794A3
Authority
WO
WIPO (PCT)
Prior art keywords
generation
low phytate
inositol polyphosphate
polyphosphate kinases
molecular disruption
Prior art date
Application number
PCT/US2004/025756
Other languages
French (fr)
Other versions
WO2005014794A2 (en
Inventor
John D York
Jill Stevenson-Paulik
Original Assignee
Univ Duke
John D York
Jill Stevenson-Paulik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Duke, John D York, Jill Stevenson-Paulik filed Critical Univ Duke
Publication of WO2005014794A2 publication Critical patent/WO2005014794A2/en
Publication of WO2005014794A3 publication Critical patent/WO2005014794A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present subject matter provides novel inositol phosphate kinase polypeptides (Ipk1 and Ipk2) and nucleic acid molecules encoding the same. The present subject matter also provides methods of using the novel polypeptides and5 nucleic acid molecules. In some embodiments, the methods provide steps for producing a low phytate plant.
PCT/US2004/025756 2003-08-07 2004-08-09 Generation of low phytate plants by molecular disruption of inositol polyphosphate kinases WO2005014794A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49326703P 2003-08-07 2003-08-07
US60/493,267 2003-08-07

Publications (2)

Publication Number Publication Date
WO2005014794A2 WO2005014794A2 (en) 2005-02-17
WO2005014794A3 true WO2005014794A3 (en) 2006-02-09

Family

ID=34135221

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/025756 WO2005014794A2 (en) 2003-08-07 2004-08-09 Generation of low phytate plants by molecular disruption of inositol polyphosphate kinases

Country Status (1)

Country Link
WO (1) WO2005014794A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005078079A1 (en) 2004-02-09 2005-08-25 Pioneer Hi-Bred International, Inc. Phytate polynucleotides and methods of use
EP2092068B1 (en) * 2006-12-14 2014-10-08 Dow AgroSciences LLC Optimized non-canonical zinc finger proteins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE GENEMBL. [online] 19 July 2000 (2000-07-19), SHINN P. ET AL: "Gene sequence.", XP002994984, accession no. stic Database accession no. (AC069252) *
DATABASE UNIPROT [online] 1 October 2000 (2000-10-01), SHINN P. ET AL: "Protein sequence from nucleic acid.", XP002994362, accession no. stic Database accession no. (Q9LM49) *
SHI J. ET AL: "The maize low-phytic acid mutant lpa2 is caused by mutation in an inositol phosphate kinase gene.", PLANT PHYSIOLOGY., vol. 131, February 2003 (2003-02-01), pages 507 - 515, XP002993216 *

Also Published As

Publication number Publication date
WO2005014794A2 (en) 2005-02-17

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