WO2005014785A3 - Conditionally-immortalized hematopoietic progenitor cell lines - Google Patents

Conditionally-immortalized hematopoietic progenitor cell lines Download PDF

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Publication number
WO2005014785A3
WO2005014785A3 PCT/US2004/019356 US2004019356W WO2005014785A3 WO 2005014785 A3 WO2005014785 A3 WO 2005014785A3 US 2004019356 W US2004019356 W US 2004019356W WO 2005014785 A3 WO2005014785 A3 WO 2005014785A3
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WO
WIPO (PCT)
Prior art keywords
cell line
generating
conditionally
hematopoietic progenitor
progenitor cell
Prior art date
Application number
PCT/US2004/019356
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French (fr)
Other versions
WO2005014785A2 (en
Inventor
Robert G Hawley
Shannon S Eaker
Teresa S Hawley
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Univ George Washington
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Publication date
Application filed by Univ George Washington filed Critical Univ George Washington
Publication of WO2005014785A2 publication Critical patent/WO2005014785A2/en
Publication of WO2005014785A3 publication Critical patent/WO2005014785A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0641—Erythrocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/14—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A cell line comprising a cellular genome, wherein the cellular genome comprises a nucleic acid sequence encoding HOX11 and an inducible promoter operably linked thereto, wherein the cell line is a hematopoietic progenitor cell line, and wherein the cell line is conditionally-immortalized; a method of generating a conditionally-immortalized hematopoietic progenitor cell line; the cell line generated thereby; a method of generating erythrocytes; a method of generating granulocytes; methods of generating monocytes/macrophages, granulocytes, and megakaryocytes; a method of generating monocytes/macrophages, granulocytes, megakaryocytes, and erythrocytes; a method of performing a blood transfusion in a host; a method of determining whether or not an agent influences hematopoiesis; and a method of screening a drug candidate for an effect on a hematopoietic cell.
PCT/US2004/019356 2003-06-18 2004-06-18 Conditionally-immortalized hematopoietic progenitor cell lines WO2005014785A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47941503P 2003-06-18 2003-06-18
US60/479,415 2003-06-18

Publications (2)

Publication Number Publication Date
WO2005014785A2 WO2005014785A2 (en) 2005-02-17
WO2005014785A3 true WO2005014785A3 (en) 2005-06-02

Family

ID=34135050

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/019356 WO2005014785A2 (en) 2003-06-18 2004-06-18 Conditionally-immortalized hematopoietic progenitor cell lines

Country Status (1)

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WO (1) WO2005014785A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE491022T1 (en) 2005-10-18 2010-12-15 Nat Jewish Health CONDITIONALLY IMMORTALIZED ADULT LONG-TERM STEM CELLS AND METHOD FOR THE PRODUCTION AND USE OF SUCH CELLS
GB0526664D0 (en) * 2005-11-30 2006-02-08 Plasticell Ltd Method
KR20140092232A (en) 2007-03-13 2014-07-23 내셔날 쥬이쉬 헬스 Methods for generation of antibodies
US8986702B2 (en) 2008-05-16 2015-03-24 Taiga Biotechnologies, Inc. Antibodies and processes for preparing the same
CN103937749B (en) * 2008-07-21 2018-04-17 泰加生物工艺学公司 Break up cytode and preparation method thereof
ES2681478T3 (en) 2008-08-28 2018-09-13 Taiga Biotechnologies, Inc. MYC modulators, methods of use thereof and methods to identify agents that modulate MYC
AU2013292330B2 (en) 2012-07-20 2018-07-12 Htyr Acquisition Llc Enhanced reconstitution and autoreconstitution of the hematopoietic compartment
US9365825B2 (en) 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
US10272115B2 (en) 2013-03-11 2019-04-30 Taiga Biotechnologies, Inc. Production and use of red blood cells
CN105106938A (en) * 2015-08-07 2015-12-02 中国人民解放军军事医学科学院野战输血研究所 Application of thrombopoietin on homing promotion of hemopoietic stem cells
WO2018102678A1 (en) 2016-12-02 2018-06-07 Taiga Biotechnologies, Inc. Nanoparticle formulations
US10149898B2 (en) 2017-08-03 2018-12-11 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of melanoma
EP3952909A4 (en) 2019-04-08 2023-06-07 Taiga Biotechnologies, Inc. COMPOSITIONS AND METHODS FOR CRYOPRESERVATION OF IMMUNE CELLS
SG11202112529WA (en) 2019-05-14 2021-12-30 Taiga Biotechnologies Inc Compositions and methods for treating t cell exhaustion
CN113025573A (en) * 2020-12-30 2021-06-25 中国人民解放军军事科学院军事医学研究院 Liquid supplementing culture medium and method for culturing and inducing hematopoietic stem cells to differentiate into megakaryocyte
EP4506448A1 (en) * 2023-08-09 2025-02-12 HemostOD SA Generation of immortalized progenitor megakaryocytes from human hematopoietic stem cells for large scale platelet production into microfluidics device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650298A (en) * 1993-06-14 1997-07-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5837507A (en) * 1995-11-13 1998-11-17 The Regents Of The University Of California Hox-induced enhancement of in vivo and in vitro proliferative capacity and gene therapeutic methods
US5874301A (en) * 1994-11-21 1999-02-23 National Jewish Center For Immunology And Respiratory Medicine Embryonic cell populations and methods to isolate such populations

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650298A (en) * 1993-06-14 1997-07-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5874301A (en) * 1994-11-21 1999-02-23 National Jewish Center For Immunology And Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5837507A (en) * 1995-11-13 1998-11-17 The Regents Of The University Of California Hox-induced enhancement of in vivo and in vitro proliferative capacity and gene therapeutic methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GREZ ET AL.: "Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells", PNAS, vol. 87, December 1990 (1990-12-01), pages 9202 - 9206, XP000578344 *
HAWLEY ET AL.: "Regulatable HOX11-mediated immortalization of hematopoietic precursors from mouse embryonic stem cells", BLOOD, vol. 102, no. 11 (ABST.NO. 582), November 2003 (2003-11-01), pages 167A *

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