WO2005003376A3 - Anthrax antigen vaccine cultures by fluorescence polarization - Google Patents

Anthrax antigen vaccine cultures by fluorescence polarization Download PDF

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Publication number
WO2005003376A3
WO2005003376A3 PCT/US2004/009386 US2004009386W WO2005003376A3 WO 2005003376 A3 WO2005003376 A3 WO 2005003376A3 US 2004009386 W US2004009386 W US 2004009386W WO 2005003376 A3 WO2005003376 A3 WO 2005003376A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence polarization
methods
antigen vaccine
antibody
bacteria
Prior art date
Application number
PCT/US2004/009386
Other languages
French (fr)
Other versions
WO2005003376A2 (en
Inventor
Malford E Cullum
Paul M Hine
Lloyd G Simonson
Chun N Shih
Diane R Bienek
Sukjoon Park
James C Ragain Jr
Original Assignee
Us Sec The Navy Naval Medical
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Sec The Navy Naval Medical filed Critical Us Sec The Navy Naval Medical
Publication of WO2005003376A2 publication Critical patent/WO2005003376A2/en
Publication of WO2005003376A3 publication Critical patent/WO2005003376A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria

Abstract

The inventive subject matter relates to a competitive method for estimating the concentration in a sample of a Bacillus anthracis protein or antibody thereof selected from the group consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). The method may employ the use of Fluorescence Polarization, FLT or FRET. The competitive methods are capable of detecting a target protein within 5 minutes within the range of 0.1 to 10.0 nM. The methods provide for the rapid detection and quantitation of bacteria, bacterial antigen or antibody in culture media or broth of growing cultures of bacteria, including B. anthracis by fluorescent methods.
PCT/US2004/009386 2003-03-28 2004-03-26 Anthrax antigen vaccine cultures by fluorescence polarization WO2005003376A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45794003P 2003-03-28 2003-03-28
US60/457,940 2003-03-28

Publications (2)

Publication Number Publication Date
WO2005003376A2 WO2005003376A2 (en) 2005-01-13
WO2005003376A3 true WO2005003376A3 (en) 2005-08-18

Family

ID=33563681

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/009386 WO2005003376A2 (en) 2003-03-28 2004-03-26 Anthrax antigen vaccine cultures by fluorescence polarization

Country Status (2)

Country Link
US (1) US7504202B2 (en)
WO (1) WO2005003376A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7601351B1 (en) 2002-06-26 2009-10-13 Human Genome Sciences, Inc. Antibodies against protective antigen
US7408640B2 (en) * 2003-11-05 2008-08-05 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization instruments and methods for detection of exposure to biological materials by fluorescence polarization immunoassay of saliva, oral or bodily fluids
US20060246079A1 (en) * 2003-11-14 2006-11-02 Morrow Phillip R Neutralizing human antibodies to anthrax toxin
WO2005081749A2 (en) * 2004-01-23 2005-09-09 Avanir Pharmaceuticals, Inc. Neutralizing human antibodies to anthraxtoxin
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
US8663926B2 (en) * 2006-02-15 2014-03-04 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services, Centers For Disease Control And Prevention Detection of anthrax pathogenicity factors
EP2021021A2 (en) 2006-05-12 2009-02-11 Oklahoma Medical Research Foundation Anthrax compositions and methods of use and production
US20080227222A1 (en) * 2007-03-13 2008-09-18 Cullum Malford E Method for the detection of target molecules by fluorescence polarization using peptide mimics
US7935345B2 (en) 2007-05-21 2011-05-03 Children's Hospital & Research Center At Oakland Monoclonal antibodies that specifically bind to and neutralize bacillus anthracis toxin, compositions, and methods of use
WO2013112834A1 (en) * 2012-01-25 2013-08-01 Vanderbilt University Compositions and methods for treating infections

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040019182A1 (en) * 2002-02-25 2004-01-29 Cunningham Barry R. Reagents and methods for assaying Bacillus anthracis lethal factor protease

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6927068B2 (en) * 2002-01-30 2005-08-09 The United States Of America As Represented By The Secretary Of The Navy Rapid and non-invasive method to evaluate immunization status of a patient

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040019182A1 (en) * 2002-02-25 2004-01-29 Cunningham Barry R. Reagents and methods for assaying Bacillus anthracis lethal factor protease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GALL D. ET AL: "Validation of the fluorescence polarization assay and comparison to other serological assays for the detection of serum antibodies to Brucella abortus in Bison", JOURNAL OF WILDLIFE DISEASES, vol. 36, no. 3, 2000, pages 469 - 476 *

Also Published As

Publication number Publication date
US7504202B2 (en) 2009-03-17
WO2005003376A2 (en) 2005-01-13
US20040235075A1 (en) 2004-11-25

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