WO2004106929A3 - Assay method - Google Patents

Assay method Download PDF

Info

Publication number
WO2004106929A3
WO2004106929A3 PCT/GB2004/002282 GB2004002282W WO2004106929A3 WO 2004106929 A3 WO2004106929 A3 WO 2004106929A3 GB 2004002282 W GB2004002282 W GB 2004002282W WO 2004106929 A3 WO2004106929 A3 WO 2004106929A3
Authority
WO
WIPO (PCT)
Prior art keywords
specific binding
binding partner
labelled
analyte
membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2004/002282
Other languages
French (fr)
Other versions
WO2004106929A2 (en
Inventor
Einar Moerk
Jostein Holtlund
Ingar Eilertsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Axis Shield ASA
Original Assignee
Axis Shield ASA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axis Shield ASA filed Critical Axis Shield ASA
Publication of WO2004106929A2 publication Critical patent/WO2004106929A2/en
Publication of WO2004106929A3 publication Critical patent/WO2004106929A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides a method for assaying for an analyte in a sample, said method comprising: applying to a porous membrane to which optionally is bound a first specific binding partner for said analyte: (i) said sample; (ii) a water-binding polymer in aqueous solution; and (iii) one or both of a second, optionally labelled specific binding partner for said analyte in aqueous solution or dispersion and a labelled ligand for a said specific binding partner; flushing said membrane; and measuring a signal from or induced by a labelled material retained on the surface of said membrane whereby to generate an indication of the content of said analyte in said sample; with the provisos that the porosity of said membrane is such that, in the absence of any said first specific binding partner bound thereto, said second specific binding partner (where used) is able to be flushed therethrough but conjugates of said second specific binding partner and said labelled ligand (where used) are not able to be flushed therethrough, that at least one of said first and second specific binding partners is used, that at least one of a said labelled second specific binding partner and a said labelled ligand is used, and that where no first specific binding partner is used then both a said second specific binding partner and a labelled ligand are used.
PCT/GB2004/002282 2003-05-29 2004-05-28 Assay method Ceased WO2004106929A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0312403A GB0312403D0 (en) 2003-05-29 2003-05-29 Assay
GB0312403.9 2003-05-29

Publications (2)

Publication Number Publication Date
WO2004106929A2 WO2004106929A2 (en) 2004-12-09
WO2004106929A3 true WO2004106929A3 (en) 2005-02-10

Family

ID=9959018

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/002282 Ceased WO2004106929A2 (en) 2003-05-29 2004-05-28 Assay method

Country Status (2)

Country Link
GB (1) GB0312403D0 (en)
WO (1) WO2004106929A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006082318A1 (en) * 2005-02-04 2006-08-10 Diagnostica Stago Immunochromatographic method for the quantitative measurement of analytes in a liquid sample
FR2881828B1 (en) * 2005-02-04 2007-11-30 Diagnostica Stago Soc Par Acti METHOD OF QUANTITATIVE MEASUREMENT BY IMMUNOCHROMATOGRAPHY, ANALYTES IN A LIQUID SAMPLE
GB0522193D0 (en) * 2005-10-31 2005-12-07 Axis Shield Asa Method

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2062224A (en) * 1979-08-20 1981-05-20 Orion Yhtymae Oy Solid-phase enzyme- immunoassay method
US4692330A (en) * 1984-09-26 1987-09-08 Amano Pharmaceutical Co., Ltd. Process for accelerating antigen-antibody reaction
EP0299428A2 (en) * 1987-07-13 1989-01-18 Abbott Laboratories Process for immunochromatography with colloidal particles
EP0310872A1 (en) * 1987-10-07 1989-04-12 Hygeia Sciences, Inc. Metal sol capture immunoassay procedure, kit for use therewith and captured metal containing composite
EP0337785A1 (en) * 1988-04-14 1989-10-18 Johnson & Johnson Clinical Diagnostics, Inc. Diagnostic kit and method for rapid detection of antibodies
WO1990001167A1 (en) * 1988-07-19 1990-02-08 E.I. Du Pont De Nemours And Company Porous support system for the immobilization of immunoassay components and assays performed therewith
US4931385A (en) * 1985-06-24 1990-06-05 Hygeia Sciences, Incorporated Enzyme immunoassays and immunologic reagents
WO1992021769A1 (en) * 1991-05-30 1992-12-10 Abbott Laboratories Reagents containing a nonspecific binding blocker in ion-capture binding assays
WO1997031259A1 (en) * 1996-02-22 1997-08-28 Dexall Biomedical Labs, Inc. Non-captive substrate liquid phase immunoassay
US5958790A (en) * 1984-12-20 1999-09-28 Nycomed Imaging As Solid phase transverse diffusion assay

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2062224A (en) * 1979-08-20 1981-05-20 Orion Yhtymae Oy Solid-phase enzyme- immunoassay method
US4692330A (en) * 1984-09-26 1987-09-08 Amano Pharmaceutical Co., Ltd. Process for accelerating antigen-antibody reaction
US5958790A (en) * 1984-12-20 1999-09-28 Nycomed Imaging As Solid phase transverse diffusion assay
US4931385A (en) * 1985-06-24 1990-06-05 Hygeia Sciences, Incorporated Enzyme immunoassays and immunologic reagents
EP0299428A2 (en) * 1987-07-13 1989-01-18 Abbott Laboratories Process for immunochromatography with colloidal particles
EP0310872A1 (en) * 1987-10-07 1989-04-12 Hygeia Sciences, Inc. Metal sol capture immunoassay procedure, kit for use therewith and captured metal containing composite
EP0337785A1 (en) * 1988-04-14 1989-10-18 Johnson & Johnson Clinical Diagnostics, Inc. Diagnostic kit and method for rapid detection of antibodies
WO1990001167A1 (en) * 1988-07-19 1990-02-08 E.I. Du Pont De Nemours And Company Porous support system for the immobilization of immunoassay components and assays performed therewith
WO1992021769A1 (en) * 1991-05-30 1992-12-10 Abbott Laboratories Reagents containing a nonspecific binding blocker in ion-capture binding assays
WO1997031259A1 (en) * 1996-02-22 1997-08-28 Dexall Biomedical Labs, Inc. Non-captive substrate liquid phase immunoassay

Also Published As

Publication number Publication date
GB0312403D0 (en) 2003-07-02
WO2004106929A2 (en) 2004-12-09

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