WO2004101755A3 - Ref1 modified plants and plant seeds - Google Patents

Ref1 modified plants and plant seeds Download PDF

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Publication number
WO2004101755A3
WO2004101755A3 PCT/US2004/014489 US2004014489W WO2004101755A3 WO 2004101755 A3 WO2004101755 A3 WO 2004101755A3 US 2004014489 W US2004014489 W US 2004014489W WO 2004101755 A3 WO2004101755 A3 WO 2004101755A3
Authority
WO
WIPO (PCT)
Prior art keywords
plant
plant cell
present
variants
gene
Prior art date
Application number
PCT/US2004/014489
Other languages
French (fr)
Other versions
WO2004101755A2 (en
Inventor
Clint Chapple
Ramesh Nair
Original Assignee
Purdue Research Foundation
Clint Chapple
Ramesh Nair
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation, Clint Chapple, Ramesh Nair filed Critical Purdue Research Foundation
Publication of WO2004101755A2 publication Critical patent/WO2004101755A2/en
Publication of WO2004101755A3 publication Critical patent/WO2004101755A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The present invention relates to plant cell wall synthesis, and to the synthesis of soluble compounds in plants commonly known as secondary metabolites or natural products. In particular, the present invention provides plant aldehyde dehydrogenases with a novel capacity of oxidizing hydroxycinnamaldehydes to hydroxycinnamic acids, and genes encoding the enzyme, as well as variants of the gene, and methods of using the genes and variants of the gene to modify plant chemistry. The methods of the present invention include reducing sinapine content of a seed, decreasing hydroxycinnamic acid content in a plant cell, and modifying lignin property in a plant cell wall by manipulating the activity of the novel plant aldehyde dehydrogenase in a plant cell.
PCT/US2004/014489 2003-05-07 2004-05-07 Ref1 modified plants and plant seeds WO2004101755A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46860803P 2003-05-07 2003-05-07
US60/468,608 2003-05-07

Publications (2)

Publication Number Publication Date
WO2004101755A2 WO2004101755A2 (en) 2004-11-25
WO2004101755A3 true WO2004101755A3 (en) 2006-02-02

Family

ID=33452216

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/014489 WO2004101755A2 (en) 2003-05-07 2004-05-07 Ref1 modified plants and plant seeds

Country Status (1)

Country Link
WO (1) WO2004101755A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101262760A (en) * 2005-08-01 2008-09-10 加拿大农业和农业食品部 Low fiber yellow canola seeds comprising high oleic, low linolenic oil
CN101454453B (en) 2006-04-05 2012-12-19 加拿大国家研究委员会 Nucleotide sequences encoding enzymes in biosynthesis of dihydroartemisinic acid
CN109055408B (en) * 2018-08-14 2021-11-23 江苏大学 BdREF2 gene for regulating and controlling anabolism of plant ferulic acid and application thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DODDING MP ET AL: "Capsid Processing Requirements for Abrogation of Fv1 and Ref1 Restriction.", JOURNAL OF VIROLOGY., vol. 79, no. 16, August 2005 (2005-08-01), pages 10571 - 1577 *
GATFIELD D ET AL: "REF1/Aly and the additional exon junction complex proteins are dispensable for nuclear mRNA export.", JCB., vol. 159, no. 4, November 2002 (2002-11-01), pages 579 - 588, XP002993212 *
JACKSON EB ET AL: "Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1).", NUCLEIC ACIDS RESEARCH., vol. 33, no. 11, June 2005 (2005-06-01), pages 3303 - 3312, XP002993213 *
NAIR R.M ET AL: "The Arabidopsis Thaliana REDUCED EPIDERMAL FLUORESCENCE1 Gene Encodes an Aldehyde Dehydrogenase Involved in Ferulic Acid and Sinapic Acid Biosynthesis.", THE PLANT CELL., vol. 16, February 2004 (2004-02-01), pages 544 - 554, XP002993209 *
NAIR RB ET AL: "Identification of a CYP84 Family of Cytochrome P450-Dependent Mono-Oxygenase Genes in Brassica Napus and Perturbation of Their Expression for Engineering sinapine Reduction in the Seeds.", PLANT PHYSIOLOGY., vol. 123, August 2000 (2000-08-01), pages 1623 - 1634, XP002993210 *
SEO YR ET AL: "Selenomethionine regulation of p53 by a ref1-dependent redox mechanism.", PNAS., vol. 99, no. 22, 29 October 2002 (2002-10-29), pages 14548 - 14553, XP002993211 *

Also Published As

Publication number Publication date
WO2004101755A2 (en) 2004-11-25

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