WO2004096972A3 - Apparatus and methods for amplification of blood stem cell numbers - Google Patents

Apparatus and methods for amplification of blood stem cell numbers Download PDF

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Publication number
WO2004096972A3
WO2004096972A3 PCT/IB2004/001736 IB2004001736W WO2004096972A3 WO 2004096972 A3 WO2004096972 A3 WO 2004096972A3 IB 2004001736 W IB2004001736 W IB 2004001736W WO 2004096972 A3 WO2004096972 A3 WO 2004096972A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
stem cell
amplification
cell numbers
blood stem
Prior art date
Application number
PCT/IB2004/001736
Other languages
French (fr)
Other versions
WO2004096972A2 (en
Inventor
Gerard Madlambayan
Peter Zandstra
Original Assignee
Insception Bioscience Inc
Gerard Madlambayan
Peter Zandstra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Insception Bioscience Inc, Gerard Madlambayan, Peter Zandstra filed Critical Insception Bioscience Inc
Publication of WO2004096972A2 publication Critical patent/WO2004096972A2/en
Publication of WO2004096972A3 publication Critical patent/WO2004096972A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Abstract

The present invention provides an apparatus and methods for eypansion of hematopoietic stem cell numners. The stem cells are cultured and differentiated cells and endogenous growth factors are removed (depleted), permitting long term culture and expansion of the stem cells. The hematopoietic stem cells are used in numerous therapeutic procedures.
PCT/IB2004/001736 2003-05-02 2004-05-03 Apparatus and methods for amplification of blood stem cell numbers WO2004096972A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US46758903P 2003-05-02 2003-05-02
US60/467,589 2003-05-02
US55427304P 2004-03-18 2004-03-18
US60/554,273 2004-03-18
US55742604P 2004-03-29 2004-03-29
US60/557,426 2004-03-29

Publications (2)

Publication Number Publication Date
WO2004096972A2 WO2004096972A2 (en) 2004-11-11
WO2004096972A3 true WO2004096972A3 (en) 2005-03-10

Family

ID=33425191

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2004/001736 WO2004096972A2 (en) 2003-05-02 2004-05-03 Apparatus and methods for amplification of blood stem cell numbers

Country Status (1)

Country Link
WO (1) WO2004096972A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017414804A1 (en) 2017-05-16 2020-01-02 Gamida-Cell Ltd. Selection and use of umbilical cord cell fractions suitable for transplantation
MX2019013613A (en) 2017-05-16 2020-07-20 Gamida Cell Ltd Selection and use of umbilical cord cell fractions suitable for transplantation.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759793A (en) * 1993-09-30 1998-06-02 Systemix, Inc. Method for mammalian cell separation from a mixture of cell populations
US5837444A (en) * 1996-07-16 1998-11-17 Shah; Kumarpal A. Islet cell transplantation machine for diabetes cure
US5925567A (en) * 1995-05-19 1999-07-20 T. Breeders, Inc. Selective expansion of target cell populations

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759793A (en) * 1993-09-30 1998-06-02 Systemix, Inc. Method for mammalian cell separation from a mixture of cell populations
US5925567A (en) * 1995-05-19 1999-07-20 T. Breeders, Inc. Selective expansion of target cell populations
US5837444A (en) * 1996-07-16 1998-11-17 Shah; Kumarpal A. Islet cell transplantation machine for diabetes cure

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BLOOD, vol. 100, no. 11, 16 November 2002 (2002-11-16), 44TH ANNUAL MEETING OF THE AMERICAN SOCIETY OF HEMATOLOGY; PHILADELPHIA, PA, USA; DECEMBER 06-10, 2002, pages Abstract No. 643, ISSN: 0006-4971 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 16 November 2002 (2002-11-16), MADLAMBAYAN GERARD J ET AL: "Manipulation of Endogenous Factor Production Enables In Vitro Stem and Progenitor Cell Expansion: Effects of Cell Selection and Medium Supplementation.", XP008041456, Database accession no. PREV200300356575 *

Also Published As

Publication number Publication date
WO2004096972A2 (en) 2004-11-11

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