WO2004090124A3 - Sequences encoding s-hydroxynitril lyases and their use in methods for the production of cyanohydrins - Google Patents

Sequences encoding s-hydroxynitril lyases and their use in methods for the production of cyanohydrins Download PDF

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Publication number
WO2004090124A3
WO2004090124A3 PCT/EP2004/003844 EP2004003844W WO2004090124A3 WO 2004090124 A3 WO2004090124 A3 WO 2004090124A3 EP 2004003844 W EP2004003844 W EP 2004003844W WO 2004090124 A3 WO2004090124 A3 WO 2004090124A3
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WO
WIPO (PCT)
Prior art keywords
cyanohydrins
production
nucleic acid
carboxylic acids
lyases
Prior art date
Application number
PCT/EP2004/003844
Other languages
French (fr)
Other versions
WO2004090124A2 (en
Inventor
Thomas Friedrich
Maria Kesseler
Bernhard Hauer
Original Assignee
Basf Ag
Thomas Friedrich
Maria Kesseler
Bernhard Hauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Thomas Friedrich, Maria Kesseler, Bernhard Hauer filed Critical Basf Ag
Publication of WO2004090124A2 publication Critical patent/WO2004090124A2/en
Publication of WO2004090124A3 publication Critical patent/WO2004090124A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/002—Nitriles (-CN)
    • C12P13/004—Cyanohydrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to new aminoacid sequences isolated from Adenia racemosa encoding S-hydroxynitrile lyases and their use in methods for the production of nitriles and/or carboxylic acids, preferably optically active nitriles and/or carboxylic acids. The invention further relates to nucleic acid encoding said S-hydroxynitrile lyases, recombinant expression constructs comprising said nucleic acid sequences, recombinant vectors comprising the nucleic acid sequences or the expression constructs, to recombinant organisms, preferably microorganisms or yeasts, comprising the nucleic acid sequences, expression constructs or vectors. The invention additionally relates to a method for the production of cyanohydrins and/or carboxylic acids, preferably optically active cyanohydrins and/or carboxylic acids from racemic aldehydes and cyanide.
PCT/EP2004/003844 2003-04-14 2004-04-13 Sequences encoding s-hydroxynitril lyases and their use in methods for the production of cyanohydrins WO2004090124A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46350003P 2003-04-14 2003-04-14
US60/463,500 2003-04-14

Publications (2)

Publication Number Publication Date
WO2004090124A2 WO2004090124A2 (en) 2004-10-21
WO2004090124A3 true WO2004090124A3 (en) 2004-11-25

Family

ID=33159878

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/003844 WO2004090124A2 (en) 2003-04-14 2004-04-13 Sequences encoding s-hydroxynitril lyases and their use in methods for the production of cyanohydrins

Country Status (1)

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WO (1) WO2004090124A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006058373A1 (en) 2006-12-08 2008-06-12 Evocatal Gmbh (R) -hydroxynitrile-lyase from Brassicaceae

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319697B1 (en) * 1998-07-02 2001-11-20 Dsm Fine Chemicals Austria Gmbh (S)-hydroxynitrile lyases with improved substrate acceptance and their use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319697B1 (en) * 1998-07-02 2001-11-20 Dsm Fine Chemicals Austria Gmbh (S)-hydroxynitrile lyases with improved substrate acceptance and their use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EFFENBERGER F: "ENZYME-CATALYZED PREPARATION AND SYNTHETIC APPLICATIONS OF OPTICALLY ACTIVE CYANOHYDRINS", CHIMIA, AARAU, CH, vol. 53, no. 12, 1999, pages 3 - 10, XP000986127, ISSN: 0009-4293 *
HICKEL A ET AL: "Hydroxynitrile lyases: Functions and properties", PHYSIOLOGIA PLANTARUM, MUNKSGAARD INTERNATIONAL PUBLISHERS, COPENHAGEN, DK, vol. 98, 1996, pages 891 - 898, XP002249788, ISSN: 0031-9317 *
JOHNSON DEAN V ET AL: "Oxynitrilases for asymmetric C-C bond formation", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 4, no. 1, February 2000 (2000-02-01), pages 103 - 109, XP002298178, ISSN: 1367-5931 *

Also Published As

Publication number Publication date
WO2004090124A2 (en) 2004-10-21

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