WO2004089295A3 - Homogeneous and heterogeneous assay methods using nucleation centers and novel covalent chemistries for the rapid measurement of phosphorylation and de-phosphorylation - Google Patents

Homogeneous and heterogeneous assay methods using nucleation centers and novel covalent chemistries for the rapid measurement of phosphorylation and de-phosphorylation Download PDF

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Publication number
WO2004089295A3
WO2004089295A3 PCT/US2004/010289 US2004010289W WO2004089295A3 WO 2004089295 A3 WO2004089295 A3 WO 2004089295A3 US 2004010289 W US2004010289 W US 2004010289W WO 2004089295 A3 WO2004089295 A3 WO 2004089295A3
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WIPO (PCT)
Prior art keywords
phosphorylation
assays
chemistries
detection
homogeneous
Prior art date
Application number
PCT/US2004/010289
Other languages
French (fr)
Other versions
WO2004089295A2 (en
Inventor
Ram S Bhatt
Allison V Miller-Wing
Rebecca Helms
Steve Gessert
Frank Gonzales
Michael J Conrad
Original Assignee
Chromagen Inc
Ram S Bhatt
Allison V Miller-Wing
Rebecca Helms
Steve Gessert
Frank Gonzales
Michael J Conrad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagen Inc, Ram S Bhatt, Allison V Miller-Wing, Rebecca Helms, Steve Gessert, Frank Gonzales, Michael J Conrad filed Critical Chromagen Inc
Publication of WO2004089295A2 publication Critical patent/WO2004089295A2/en
Publication of WO2004089295A3 publication Critical patent/WO2004089295A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/14—Peptides being immobilised on, or in, an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Novel chemistries, methods and assays are described for the rapid detection and measurement of phosphorylation or de-phosphorylation of polypeptides, oligonucleotides or lipids based upon the use of phosphoryl, phosphoroamino or phosphorothioate specific hetero-bifunctional linkers combined with fluorescence polarization or fluorogenic detection. Homogeneous assay applications possible using these novel chemistries and methods include in vitro fluorescence polarization assays for the detection of general and isoform­ specific polypeptide, oligonucleotide and lipid kinase and phosphatase enzymes. Heterogeneous assay applications possible using the same novel chemistries and assaymethods enable development of in vitro and in vivo fluorogenic assays of general or specific kinase or phosphatase enzyme activities in cell lysates or serum. In all cases, these new chemistries, methods and assays enable simultaneous multi-kinase detection and profiling.
PCT/US2004/010289 2003-04-01 2004-04-01 Homogeneous and heterogeneous assay methods using nucleation centers and novel covalent chemistries for the rapid measurement of phosphorylation and de-phosphorylation WO2004089295A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45988603P 2003-04-01 2003-04-01
US60/459,886 2003-04-01

Publications (2)

Publication Number Publication Date
WO2004089295A2 WO2004089295A2 (en) 2004-10-21
WO2004089295A3 true WO2004089295A3 (en) 2005-08-04

Family

ID=33159702

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/010289 WO2004089295A2 (en) 2003-04-01 2004-04-01 Homogeneous and heterogeneous assay methods using nucleation centers and novel covalent chemistries for the rapid measurement of phosphorylation and de-phosphorylation

Country Status (1)

Country Link
WO (1) WO2004089295A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103667205B (en) * 2013-12-10 2015-06-17 潍坊医学院 Biologically labeled bimolecular site-directed biotinylated recombinant alkaline phosphatase (AP)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0202758A1 (en) * 1985-04-17 1986-11-26 Zeneca Limited Hybridization method and probes therefor
US6130323A (en) * 1996-05-15 2000-10-10 Biogenex Laboratories Non-nucleotide linking reagents

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0202758A1 (en) * 1985-04-17 1986-11-26 Zeneca Limited Hybridization method and probes therefor
US6130323A (en) * 1996-05-15 2000-10-10 Biogenex Laboratories Non-nucleotide linking reagents

Also Published As

Publication number Publication date
WO2004089295A2 (en) 2004-10-21

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