WO2004086948A3 - A confocal microscope system for real-time simultaneous temporal measurements of metachronal wave period and ciliary beat frequency - Google Patents

A confocal microscope system for real-time simultaneous temporal measurements of metachronal wave period and ciliary beat frequency Download PDF

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Publication number
WO2004086948A3
WO2004086948A3 PCT/US2004/009504 US2004009504W WO2004086948A3 WO 2004086948 A3 WO2004086948 A3 WO 2004086948A3 US 2004009504 W US2004009504 W US 2004009504W WO 2004086948 A3 WO2004086948 A3 WO 2004086948A3
Authority
WO
WIPO (PCT)
Prior art keywords
beat frequency
real
confocal microscope
ciliary beat
microscope system
Prior art date
Application number
PCT/US2004/009504
Other languages
French (fr)
Other versions
WO2004086948A2 (en
Inventor
Lid B Wong
Donovan B Yeates
Hua Mao
Tarun Chandra
Original Assignee
Bio Techplex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Techplex Corp filed Critical Bio Techplex Corp
Priority to US10/550,124 priority Critical patent/US20060256342A1/en
Publication of WO2004086948A2 publication Critical patent/WO2004086948A2/en
Publication of WO2004086948A3 publication Critical patent/WO2004086948A3/en

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G02B21/0084—Details of detection or image processing, including general computer control time-scale detection, e.g. strobed, ultra-fast, heterodyne detection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid

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  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Dental Tools And Instruments Or Auxiliary Dental Instruments (AREA)

Abstract

The present invention relates to a method and system that enables continuous real-time analysis of both ciliary beat frequency and metachronal wave frequency from a single spot (107) in excised native ciliated epithelial tissues as well as in primary and subsequent epithelial cultures. Such method and system utilizes the concept of time-scale wavelet analysis and Hilbert Transformation (112) for backscattered light derived from a confocal (conjugate) spot on the moving cilia. This light contains inherent high and low frequency components corresponding to CBF and MWF.
PCT/US2004/009504 2003-03-28 2004-03-26 A confocal microscope system for real-time simultaneous temporal measurements of metachronal wave period and ciliary beat frequency WO2004086948A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US10/550,124 US20060256342A1 (en) 2003-03-28 2004-03-26 Confocal microscope system for real-time simultaneous temporal measurements of metachronal wave period and ciliary beat frequency

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45869803P 2003-03-28 2003-03-28
US60/458,698 2003-03-28

Publications (2)

Publication Number Publication Date
WO2004086948A2 WO2004086948A2 (en) 2004-10-14
WO2004086948A3 true WO2004086948A3 (en) 2005-06-09

Family

ID=33131817

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/009504 WO2004086948A2 (en) 2003-03-28 2004-03-26 A confocal microscope system for real-time simultaneous temporal measurements of metachronal wave period and ciliary beat frequency

Country Status (2)

Country Link
US (1) US20060256342A1 (en)
WO (1) WO2004086948A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5063005B2 (en) * 2006-02-01 2012-10-31 株式会社ジェイテクト Sound or vibration abnormality diagnosis method and sound or vibration abnormality diagnosis device
WO2011064775A1 (en) 2009-11-25 2011-06-03 Hanoch Kislev Probing system for measuring the direction and speed of mucus flow in vivo
US20130018256A1 (en) * 2009-11-25 2013-01-17 Hanoch Kislev Probing system for measuring the direction and speed of mucus flow in vivo
JP6016495B2 (en) * 2012-07-13 2016-10-26 国立大学法人九州大学 Scanning microscope
WO2014058891A1 (en) * 2012-10-09 2014-04-17 The University Of North Carolina At Chapel Hill Methods and apparatus for culturing ciliated cells
EP3647765A1 (en) * 2018-10-31 2020-05-06 INESC TEC - Instituto de Engenharia de Sistemas e Computadores, Tecnologia e Ciência Device and method for detecting and identifying extracellular vesicles in a liquid dispersion sample

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6201608B1 (en) * 1998-03-13 2001-03-13 Optical Biopsy Technologies, Inc. Method and apparatus for measuring optical reflectivity and imaging through a scattering medium
US6847456B2 (en) * 2000-04-28 2005-01-25 Massachusetts Institute Of Technology Methods and systems using field-based light scattering spectroscopy

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7365858B2 (en) * 2001-12-18 2008-04-29 Massachusetts Institute Of Technology Systems and methods for phase measurements
US7557929B2 (en) * 2001-12-18 2009-07-07 Massachusetts Institute Of Technology Systems and methods for phase measurements
US6934035B2 (en) * 2001-12-18 2005-08-23 Massachusetts Institute Of Technology System and method for measuring optical distance

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6201608B1 (en) * 1998-03-13 2001-03-13 Optical Biopsy Technologies, Inc. Method and apparatus for measuring optical reflectivity and imaging through a scattering medium
US6847456B2 (en) * 2000-04-28 2005-01-25 Massachusetts Institute Of Technology Methods and systems using field-based light scattering spectroscopy

Also Published As

Publication number Publication date
US20060256342A1 (en) 2006-11-16
WO2004086948A2 (en) 2004-10-14

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