WO2004082674A1 - Carnitine retention - Google Patents
Carnitine retention Download PDFInfo
- Publication number
- WO2004082674A1 WO2004082674A1 PCT/GB2004/001256 GB2004001256W WO2004082674A1 WO 2004082674 A1 WO2004082674 A1 WO 2004082674A1 GB 2004001256 W GB2004001256 W GB 2004001256W WO 2004082674 A1 WO2004082674 A1 WO 2004082674A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- carnitine
- agent
- carbohydrate
- substance
- derivative
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7004—Monosaccharides having only carbon, hydrogen and oxygen atoms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/205—Amine addition salts of organic acids; Inner quaternary ammonium salts, e.g. betaine, carnitine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
- A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
Definitions
- This invention relates to carnitine retention in biological tissue. More particularly, but not exclusively, the invention relates to compositions and methods of increasing carnitine retention in the animal and/or human body.
- carnitine is essential in muscle metabolism and function.
- the muscle store of carnitine is important for energy production in muscle. If the store of carnitine declines, the function of the muscle can be impaired. Indeed, patients with muscle carnitine deficiency experience premature fatigue and weakness.
- compositions for influencing carnitine retention in biological tissue comprising a carnitine substance and an agent to increase sodium- potassium ATPase pump activity in the tissue.
- the invention further provides a composition for influencing carnitine transport into biological tissue, the composition comprising a carnitine substance to increase blood/plasma carnitine concentration and an agent to increase the activity of a carnitine transport protein.
- a composition for increasing carnitine retention in the animal and/or human body the composition comprising a carnitine substance and an agent to increase blood/plasma insulin concentration.
- the invention also provides a method of influencing carnitine retention in biological tissue, in particular tissue of the animal and/or human body, the method comprising administering to the tissue a carnitine substance and an agent operable to increase sodium-potassium ATPase pump activity in the tissue.
- the invention further provides a method of increasing carnitine retention in the animal and/or human body, the method comprising administering to the body a carnitine substance and an agent to increase blood/plasma insulin concentration.
- the invention still further provides a method of influencing carnitine transport into biological tissue, the method comprising administering to the body a carnitine substance to increase blood/plasma carnitine concentration and an agent to increase the activity of a carnitine transport protein.
- the method increases carnitine retention in the tissue by increasing the transportation of the carnitine substance, or a derivative thereof into tissue cells.
- transportation is increased by stimulation of a sodium dependent transport protein and substantially simultaneously increasing blood/plasma carnitine concentration.
- the agent is operable to increase sodium dependent carnitine uptake into tissue cells, in particular skeletal muscle, liver and/or kidney cells.
- the agent may be operable to increase insulin activity in the tissue, desirably by increasing the amount of insulin in the blood/plasma.
- the agent may comprise carbohydrate or an active derivative thereof. Alternatively, or in addition, the agent may comprise amino acid and/or protein.
- the method involves oral administration and desirably ingestion of the carnitine substance and agent, desirably but not necessarily simultaneously.
- a food supplement comprising a carnitine substance and an agent as described in any of the preceding paragraphs.
- the invention further provides a composition for use in the manufacture of a medicament for influencing carnitine retention in biological tissue, the composition comprising a carnitine substance and an agent to increase sodium- potassium ATPase pump activity in the tissue.
- the invention also provides a composition for use in the manufacture of medicament for influencing carnitine transport into biological tissue, the composition comprising a carnitine substance to increase blood/plasma carnitine concentration and an agent to increase the activity of a carnitine transport protein.
- compositions for use in the manufacture of a medicament to influence carnitine retention in the animal and/or human body comprising a carnitine substance and an agent to stimulate insulin release and activity in the body.
- the invention also relates to the use of a carnitine substance and an agent as described in any of the preceding paragraphs for influencing carnitine retention in human and/or animal tissue.
- Carnitine is also provided for use in a method substantially as described in any of the paragraphs above.
- a kit is provided according to this invention comprising a carnitine substance and an agent substantially as described in any of the paragraphs above.
- a carnitine substance for use in administration to the human and/or animal body with an agent as described in any of the paragraphs above.
- the carnitine substance comprises one or more of carnitine, a functional equivalent of carnitine, an active derivative of carnitine or carnitine analogue.
- a preferred embodiment may comprise one or more of L-carnitine, a functional equivalent of L-carnitine, an active derivative of L-carnitine or an analogue thereof.
- the agent is a carbohydrate or a derivative of a carbohydrate.
- the carbohydrate is preferably a simple carbohydrate, which may be a simple sugar.
- the carbohydrate comprises glucose, but other sugars can be used, for example sucrose or fructose.
- the amount by weight of carbohydrate is administered to one unit of carnitine substance.
- the amount by weight of carbohydrate is administered to one unit of carnitine substance.
- at least 0.25g of the carnitine substance is administered, preferably with at least 2.5g of the agent.
- substantially 0.25g of the carnitine substance is administered desirably with between substantially 2.5g and 37.5g of the agent, preferably between substantially 2.5g and 23.75g, and more preferably between substantially 2.5g and 10g of the agent.
- substantially 3g of the carnitine substance is administered, desirably with between substantially 30g to 450g of the agent, preferably between substantially 230g and 285g, and more preferably between 30g and 120g of the agent.
- substantially 0.25g to 3 g of the carnitine substance is administered, desirably with a total of between substantially 2.5g to 450 g of the agent, preferably between substantially 2.5g and 285 g, and more preferably between substantially 2.5g and 120 g of the agent.
- the agent may be administered to achieve substantially simultaneous elevation of insulin and carnitine concentrations in the blood/plasma.
- the composition may be provided in a solution which may be an aqueous solution.
- Fig. 1 shows serum insulin concentrations for Example I following carnitine ingestion with Control (o) and CHO (•);
- Fig. 2 shows urinary total carnitine (TC), free carnitine (FC) and acylcamitine (AC) excretion in mg for Example I over 24 hours following carnitine ingestion with Control (D) and CHO ( ⁇ );
- Fig. 3 shows plasma TC concentration for Example I measured over 7 hours following carnitine ingestion with Control ( ⁇ ) and CHO ( ⁇ ).
- the arrows A, B, C, D indicate time of ingestion of drink;
- Fig. 4 shows the area under the plasma-time curves (AUC) for Example I for total carnitine (TC), free carnitine (FC) and acylcamitine (AC) measured over 7 hours following carnitine ingestion with Control ( ⁇ ) and CHO ( ⁇ ).
- AUC plasma-time curves
- Fig. 5 is a block diagrammatic representation of the study protocol of Example II;
- Fig. 6 shows serum insulin concentrations for Example II during 6 hour intravenous insulin infusions of 5 (D), 30 ( ⁇ ), 55 (o) and 105 (•) mU m "2 . min " ⁇ combined with a 5 hour intravenous 60 mM L-carnitine infusion;
- Fig. 7 shows plasma total carnitine concentrations during 6 hour intravenous insulin infusions of 5 (D), 30 ( ⁇ ), 55 (o) and 105 (•) mU .m “2 .min “1 ;
- Fig. 8 shows the plasma carnitine data for Example II for the 5 (o) and 105
- FIG. 9 shows plasma a total carnitine concentration of Example III during 6 hour intravenous infusion of 5 (o) and 105 (•) mU.m “2 .min "1 combined with a 5 hour intravenous 60 mM L-carnitine infusion;
- Fig. 10 shows muscle acy, acetyl and free carnitine concentrations for Example III pre and post 6 hour intravenous insulin infusions of 5 and 105 mU.m “2 .min "1 combined with a 5 hour intravenous 60mM L-carnitine infusion.
- the invention provides a composition, methodology and uses of a composition to influence carnitine retention in tissue such as muscle, liver and kidney tissue in the animal and/or human body which comprise a carnitine substance and an agent to increase blood/plasma insulin concentration with a view to increasing sodium-potassium ATPase pump activity in tissue, and thereby sodium dependent carnitine transport.
- the carnitine substance comprises one or more of L-carnitine, a functional equivalent of L-carnitine, an active derivative of L-carnitine or an analogue thereof.
- the agent can be anything which acts to increase insulin concentration, including amino acids and protein.
- the agent is a carbohydrate such as a sugar, for example glucose which acts to stimulate insulin production in the body.
- the study protocol utilised a blind crossover design where subjects acted as their own controls. Following an overnight fast, subjects reported to the laboratory on two occasions, separated by a 2 week "wash out” period to ensure similar basal muscle carnitine concentrations among experimental treatments. • On arrival, subjects voided their bladder and were asked to rest in a supine position on a bed while a cannula was inserted retrogradely into a superficial vein on the dorsal surface of the non-dominant hand. This hand was kept in a hand- warming unit (air temperature 55°C) to arterialise the venous drainage of the hand and a saline drip was attached to keep the cannula patent.
- a hand- warming unit air temperature 55°C
- Subjects abstained from the consumption of meat, dairy produce, alcohol, and strenuous exercise 24 hours before each visit and for a 24 hour period after the consumption of the carnitine solution. It was essential that subjects had a minimal intake of carnitine in their diet during this period; therefore, food was supplied to the subjects as a ready-made meal, free from carnitine.
- Urine was collected in 5 litre bottles, containing 5 ml of 10% thymol/isopropanol preservative, for 24 h following the consumption of the carnitine drink and returned to the laboratory the following morning where a final blood sample was taken. The 24 h volume was recorded and 5 ml aliquots were removed and stored at -20°C to be analysed for free and total carnitine concentrations at a later date.
- the method used for the determination of carnitine is based on the carnitine acetyltransferase (CAT) catalysed reaction:
- L-carnitine for use in the standards was purchased from Sigma Chemical Co., St. Louis, Mo. U.S.A., as was the unlabelled acetyl-coenzyme A (sodium salt, purity 90-95%), N-ethylmalemide, and the Dowex 1X 8 (200-400 mesh, Cl " form).
- [ 14 C]acetyl-coenzyme A was obtained from Amersham, Buckinghamshire, UK (specific radioactivity 10 ⁇ Ci).
- Carnitine acetyltransferase (5 mg/ml) was obtained from Roche Molecular Biochemicals, East London, U.K. and scintillation liquid (Scintillator Plus) was purchased from Packard Biosciences, Groninger, The Netherlands.
- Radioenzymatic analysis of carnitine was carried out by adding twenty-five ⁇ l phosphate buffer (1 M, pH 6.5), 25 ⁇ l acetyl-CoA (300 ⁇ M), 10 ⁇ l NEM (40 mM), and 25 ⁇ l ( 14 C]acetyl-CoA (4 ⁇ M) to each tube.
- Carnitine acetyltransferase (CAT) diluted 1 :10 was then defrosted and 20 ⁇ l were added to each sample at 20 s intervals.
- each tube was transferred to a column of Dowex 1X 8 contained in a Pasteur pipette at 20 s intervals using an automatic pipette (Microlab 1000, Hamilton, Bonaduz, Switzerland).
- the pipette aspirated each 240 ⁇ l sample with a 10 ⁇ l air gap, mixed with 250 ⁇ l water (Millipor) and then voided the solution into the top of column.
- the tube was then washed with 250 ⁇ l water (Millipor), which were then aspirated, mixed with 250 ⁇ l water (Millipor) and voided into the same column.
- the effluent was collected into 20 ml vials and mixed with 10 ml scintillation fluid, ⁇ -radioactivity of each of the vials was counted for 3 min.
- a two-way ANOVA time and treatment effects, SPSS version 10, USA was performed to detect differences in plasma carnitine and serum insulin.
- a Student's paired .-test was used to locate differences in 24 h urinary carnitine content and area under plasma time curved between treatments.
- the total area under the plasma carnitine-time curve was calculated using KaleidaGraph (version 3.51 , Synergy Software, USA). Statistical significance was declared at P ⁇ 0.05, and all the values are means ⁇ SE.
- Fig. 2 shows a graph of urinary total carnitine (TC), free carnitine (FC) and acylcamitine (AC) excretion in mg over a period of 24 hours following an oral dose of 3.01 g of L-carnitine ingestion with control (CON) and carbohydrate (CHO).
- Fig. 3 shows a plot of plasma total carnitine concentration measured over
- Fig. 4 shows a plot of the area under the plasma-time curves (AUC) for total carnitine (TC), free carnitine (FC) and acylcamitine (AC) measured over a 7 hour period following an oral dose of 3.01 g L-carnitine with Control (CON) and carbohydrate (CHO).
- AUC area under the plasma-time curves
- TC total carnitine
- FC free carnitine
- AC acylcamitine
- Insulin increases carnitine retention most probably by increasing sodium-potassium ATPase pump activity and, thus, sodium dependent transport of carnitine into cells (particularly skeletal and cardiac muscle). Insulin may also enable more FC to be available to tissues by 1 ) inhibiting acylation of supplemented L-carnitine and/or 2) by stimulating carnitine retention by reabsorption by the kidney.
- This hand was kept in a hand-warming unit (air temperature 55°C) to arterialise the venous drainage of the hand and a saline drip was attached to keep the cannula patent.
- a second cannula was placed in an antecubital vein in the non-dominant forearm for the infusion of insulin and glucose and a third cannula was inserted into an antecubital vein in the opposite arm for infusion of L-carnitine.
- a bolus dose of 15mg - kg "1 L-L-camitine was administered intravenously over a 10 min period in order to achieve a plasma concentration of - 500 ⁇ mol / 1. This was followed by a constant infusion at 10mg . kg "1 . h "1 for the next 290 min to maintain a supraphysiological steady steady state plasma carnitine concentration.
- t 360 both insulin and L-carnitine infusions were stopped and subjects were free to leave the laboratory once their blood glucose levels had stabilised.
- euglycaemia was maintained throughout insulin infusion by means of simultaneous infusion of a 20% glucose solution.
- 1 ml of arterialized venous (a-v) blood was obtained every 5min to determine blood glucose concentration on-line (YSI 2300 STATplus, YSI, Yellow Springs, OH).
- 5 ml of a-v blood were obtained every half hour (and at 80 min) for 6 h. Two ml of this blood were collected into lithium heparin containers and, after centrifugation (14,000 rpm for 2 min), the plasma was removed and immediately frozen in liquid nitrogen.
- a two-way ANOVA time and treatment effects, GraphPad Prism version 3, GraphPad Software, Inc., USA was performed to detect differences in plasma carnitine and serum insulin concentrations. If significance was achieved, a repeated measure ANOVA (GraphPad Prism version 3, GraphPad Software, Inc., USA) was used to locate differences between treatments at each time point. Statistical significance was declared at P ⁇ 0.05, and all values presented represent mean ⁇ standard error (SE).
- Plasma total carnitine (TC) concentration before and throughout the 300 min of 60 mM L-carnitine infusion during each fo the four euglycaemic insulin clamps (5, 30, 55, and 105 mU . m “2 . min "1 ) is shown in Fig. 7.
- the basal plasma TC concentration was similar across experimental groups, (i.e. 47.7 ⁇ 0.6 ⁇ mol / 1).
- Fig. 7 shows the plasma total carnitine concentrations during 6 h intravenous insulin infusions of 5(D), 30 ( ⁇ ), 55 (o), and 105 (•) mU . m “2 . min "1 combined with a 5 h intravenous 60 mM L-camitine infusion. Values are means ⁇ SE expressed in ⁇ mol/l.
- Fig. 8 shows the plasma carnitine data for only the 5 and 105 mU . m “2 . min "1 doses. As can be seen, plasma carnitine concentration was siginificantly lower during the final two hours of infusion at the highest dose.
- the study protocol was as follows. Subjects reported to the laboratory in the morning after an overnight fast and underwent exactly the same experimental procedures as described in the previous Example II study protocol. However, on this occasion two, as opposed to four, euglycaemic insulin clamps (5 and 105 mU . m “2 . min "1 ) were performed in a randomised order, and each was seprated by 2 weeks. Each clamp was maintained for 6 hours and a muscle biopsy sample was obtained from the quadriceps muscle group in the basal state (prior) to infusion of carnitine and glucose and insulin) and after 6 hrs of infusion.
- euglycaemic insulin clamps 5 and 105 mU . m "2 . min "1 ) were performed in a randomised order, and each was seprated by 2 weeks. Each clamp was maintained for 6 hours and a muscle biopsy sample was obtained from the quadriceps muscle group in the basal state (prior) to infusion of carnitine and glucose and insulin) and after 6
- Fig. 9 shows plasma carnitine concentration during the 5 and 105 mU . m “2 . min " insulin clamps.
- Fig. 9 shows the plasma total carnitine concentrations during 6 h intravenous insulin infusions of 5 (o) and 105 (•) 105 mU . m “2 . min "1 combined with a 5 h intravenous 60 mM L-carnitine infusion. Values are means ⁇ SE expressed in ⁇ mol / 1.
- Fig. 10 shows muscle acyl, acetyl and free carnitine concentrations (sum equals muscle total carnitine concentration) pre and post 6 h intravenous insulin infusions of 5 and 105 (•) mU . m “2 . min "1 combined with a 5 h intravenous 60 mM L-carnitine infusion. Values are means ⁇ SE expressed in ⁇ mol / 1.
- the lowest insulin infusion rate (equivalent to fasting insulin concentration) had no effect on muscle carnitine accumulation. However, the highest infusion rate resulted in a significant increase in muscle total carnitine concentration.
- Insulin promotes muscle carnitine accumulation in the presence of elevated plasma carnitine concentrations. This is the first demonstration that insulin can have such an effect. We believe this response is achieved via the stimulatory effect of insulin on sodium dependent muscle carnitine transport.
- the present invention therefore has useful application in increasing carnitine retention in muscle tissue and thereby reducing the metabolic effects of depleted free carnitine in muscle during exercise, and including the effect on muscle fatigue and muscular performance.
- Other modifications may be made without departing from the scope of the present invention.
- Other agents may be used which stimulate carnitine retention primarily by way of increasing carnitine transport into tissue, such as insulin or active derivatives thereof.
- Other agents may include, either as an alternative or as an addition, amino acid(s) and protein(s). Active derivatives, variants or analogues of carnitine may be used.
- the composition may be administered in any convenient form such as tablet, powder, pellet or the like and otherwise than by ingestion, such as injection.
- agent such as carbohydrate
- the invention can be used to increase carnitine retention in animal as well as human bodies, and in whole bodies, tissues or cells derived therefrom.
- the invention also provides a kit comprising a carnitine substance and an agent such as a carbohydrate, as described above.
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Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/549,384 US9662344B2 (en) | 2003-03-20 | 2004-03-22 | Carnitine retention |
CA2519253A CA2519253C (en) | 2003-03-20 | 2004-03-22 | Carnitine retention |
EP04722325A EP1603552A1 (en) | 2003-03-20 | 2004-03-22 | Carnitine retention |
AU2004222508A AU2004222508B2 (en) | 2003-03-20 | 2004-03-22 | Carnitine retention |
US14/444,151 US20140378397A1 (en) | 2003-03-20 | 2014-07-28 | Carnitine Retention |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0306394.8 | 2003-03-20 | ||
GBGB0306394.8A GB0306394D0 (en) | 2003-03-20 | 2003-03-20 | Carnitine retention |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/549,384 A-371-Of-International US9662344B2 (en) | 2003-03-20 | 2004-03-22 | Carnitine retention |
US14/444,151 Continuation US20140378397A1 (en) | 2003-03-20 | 2014-07-28 | Carnitine Retention |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2004082674A1 true WO2004082674A1 (en) | 2004-09-30 |
WO2004082674A9 WO2004082674A9 (en) | 2004-12-23 |
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ID=9955155
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2004/001256 WO2004082674A1 (en) | 2003-03-20 | 2004-03-22 | Carnitine retention |
Country Status (6)
Country | Link |
---|---|
US (2) | US9662344B2 (en) |
EP (2) | EP2517703A1 (en) |
AU (1) | AU2004222508B2 (en) |
CA (1) | CA2519253C (en) |
GB (1) | GB0306394D0 (en) |
WO (1) | WO2004082674A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130095125A1 (en) * | 2005-03-19 | 2013-04-18 | Bruce W. Kneller | Palatinose for enhancing dietary supplement and pharmaceutical delivery |
WO2014135888A1 (en) * | 2013-03-07 | 2014-09-12 | The University Of Nottingham | Composition and use |
Citations (1)
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WO2001095915A1 (en) * | 2000-06-14 | 2001-12-20 | Sigma-Tau Healthscience S.P.A. | Dietary supplement enhancing the muscular energy metabolism, comprising an alkanoyl carnitine and ribose |
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IT1120033B (en) * | 1979-10-05 | 1986-03-19 | Sigma Tau Ind Farmaceuti | INCLUDING PHARMACEUTICAL COMPOSITION-CARNITINE SUITABLE FOR PARENTERAL FEEDING |
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JPS62198621A (en) * | 1986-02-24 | 1987-09-02 | Kanebo Ltd | Carcinostatic assistant |
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KR100255039B1 (en) * | 1997-07-28 | 2000-05-01 | 박영구 | Process for the preparation of l-carnitine |
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2003
- 2003-03-20 GB GBGB0306394.8A patent/GB0306394D0/en not_active Ceased
-
2004
- 2004-03-22 EP EP12166463A patent/EP2517703A1/en not_active Withdrawn
- 2004-03-22 EP EP04722325A patent/EP1603552A1/en not_active Withdrawn
- 2004-03-22 AU AU2004222508A patent/AU2004222508B2/en not_active Ceased
- 2004-03-22 WO PCT/GB2004/001256 patent/WO2004082674A1/en active Search and Examination
- 2004-03-22 US US10/549,384 patent/US9662344B2/en active Active
- 2004-03-22 CA CA2519253A patent/CA2519253C/en not_active Expired - Fee Related
-
2014
- 2014-07-28 US US14/444,151 patent/US20140378397A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2001095915A1 (en) * | 2000-06-14 | 2001-12-20 | Sigma-Tau Healthscience S.P.A. | Dietary supplement enhancing the muscular energy metabolism, comprising an alkanoyl carnitine and ribose |
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US20130095125A1 (en) * | 2005-03-19 | 2013-04-18 | Bruce W. Kneller | Palatinose for enhancing dietary supplement and pharmaceutical delivery |
US10624915B2 (en) | 2005-03-19 | 2020-04-21 | Bruce W. Kneller | Palatinose for enhancing dietary supplement and pharmaceutical delivery |
US11103522B2 (en) | 2005-03-19 | 2021-08-31 | Bruce W. Kneller | Palatinose for enhancing dietary supplement and pharmaceutical delivery |
WO2014135888A1 (en) * | 2013-03-07 | 2014-09-12 | The University Of Nottingham | Composition and use |
Also Published As
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GB0306394D0 (en) | 2003-04-23 |
WO2004082674A9 (en) | 2004-12-23 |
CA2519253A1 (en) | 2004-09-30 |
EP1603552A1 (en) | 2005-12-14 |
US9662344B2 (en) | 2017-05-30 |
CA2519253C (en) | 2012-10-16 |
EP2517703A1 (en) | 2012-10-31 |
US20060240075A1 (en) | 2006-10-26 |
AU2004222508A1 (en) | 2004-09-30 |
US20140378397A1 (en) | 2014-12-25 |
AU2004222508B2 (en) | 2010-07-15 |
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