WO2004081179A3 - Regulation of rna stability - Google Patents

Regulation of rna stability Download PDF

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Publication number
WO2004081179A3
WO2004081179A3 PCT/US2004/006703 US2004006703W WO2004081179A3 WO 2004081179 A3 WO2004081179 A3 WO 2004081179A3 US 2004006703 W US2004006703 W US 2004006703W WO 2004081179 A3 WO2004081179 A3 WO 2004081179A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
processing
cell
parn
variant
Prior art date
Application number
PCT/US2004/006703
Other languages
French (fr)
Other versions
WO2004081179A2 (en
Inventor
Wi S Lai
Perry J Blackshear
Original Assignee
Us Gov Health & Human Serv
Wi S Lai
Perry J Blackshear
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, Wi S Lai, Perry J Blackshear filed Critical Us Gov Health & Human Serv
Publication of WO2004081179A2 publication Critical patent/WO2004081179A2/en
Publication of WO2004081179A3 publication Critical patent/WO2004081179A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates, e.g., to a method for screening an agent for the ability to modulate RNA processing. The agent is contacted with a sample comprising i) tristetraprolin (TTP) or a related protein, or an active fragment or variant thereof, ii) a poly(A) ribonuclease (PARN) polypeptide, or an active fragment or variant thereof, wherein, if the PARN is in a cell, the cell comprises exogenous PARN, and iii) an RNA which comprises an AU-rich element (ARE) downstream of a reporter sequence, and a 3' poly(A) tail, wherein processing of the RNA can be detected, under conditions effective for processing and, optionally, translation of the RNA; and a parameter associated with processing of the RNA is detected. The method can be conducted in a cell-free (in vitro) or cell-based format.
PCT/US2004/006703 2003-03-06 2004-03-05 Regulation of rna stability WO2004081179A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45197603P 2003-03-06 2003-03-06
US60/451,976 2003-03-06

Publications (2)

Publication Number Publication Date
WO2004081179A2 WO2004081179A2 (en) 2004-09-23
WO2004081179A3 true WO2004081179A3 (en) 2005-02-10

Family

ID=32990636

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/006703 WO2004081179A2 (en) 2003-03-06 2004-03-05 Regulation of rna stability

Country Status (1)

Country Link
WO (1) WO2004081179A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017066796A2 (en) * 2015-10-16 2017-04-20 The Children's Medical Center Corporation Modulators of telomere disease
WO2017066712A2 (en) 2015-10-16 2017-04-20 The Children's Medical Center Corporation Modulators of telomere disease

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6187543B1 (en) * 1996-05-16 2001-02-13 Duke University Method of screening a compound for its ability to enhance the ability of tristetraprolin to inhibit tumor necrosis factor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6187543B1 (en) * 1996-05-16 2001-02-13 Duke University Method of screening a compound for its ability to enhance the ability of tristetraprolin to inhibit tumor necrosis factor

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BLACKSHEAR P.J.: "Tristetraprolin and other CCCH tandem zinc-finger proteins in the regulation of mRNA turnover.", BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 30, no. 6, November 2002 (2002-11-01), pages 945 - 952, XP002302923, ISSN: 0300-5127 *
CARBALLO E. ET AL.: "Evidence that tristetraprolin is a physiological regulator of granulocyte-macrophage colony-stimulating factor messenger RNA deadenylation and stability", BLOOD, W.B. SAUNDERS CO., ORLANDO, FL, US, vol. 95, no. 6, 15 March 2000 (2000-03-15), pages 1891 - 1899, XP002160579, ISSN: 0006-4971 *
CARBALLO E. ET AL.: "Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin", SCIENCE, vol. 281, no. 5379, 14 August 1998 (1998-08-14), pages 1001 - 1005, XP002160577, ISSN: 0036-8075 *
CARBALLO E. ET AL.: "TRISTETRAPROLIN IS A REGULATOR OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR MRNA STABILITY", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 28, no. 7, SUPPL 1, 10 July 2000 (2000-07-10), pages 36, XP000982275, ISSN: 0301-472X *
CHEN C.-Y. ET AL.: "AU binding proteins recruit the exosome to degrade ARE-containing mRNAs", CELL, vol. 107, no. 4, 16 November 2001 (2001-11-16), pages 451 - 464, XP002302920, ISSN: 0092-8674 *
CHEN C.-Y.A. ET AL.: "AU-rich elements: characterization and importance in mRNA degradation", TIBS TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER PUBLICATION, CAMBRIDGE, EN, vol. 20, no. 11, November 1995 (1995-11-01), pages 465 - 470, XP004222338, ISSN: 0968-0004 *
LAI W.S. ET AL.: "Evidence that tristetraprolin binds to AU-rich elements and promotes the deadenylation and destabilization of tumor necrosis factor alpha mRNA", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 19, no. 6, June 1999 (1999-06-01), pages 4311 - 4323, XP002160578, ISSN: 0270-7306 *
LAI W.S. ET AL.: "Interactions of CCCH zinc finger proteins with mRNA. Binding of tristetraprolin-related zinc finger proteins to Au-rich elements and destabilization of mRNA", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 23, 9 June 2000 (2000-06-09), pages 17827 - 17837, XP002160580, ISSN: 0021-9258 *
LAI W.S. ET AL.: "Interactions of CCCH zinc finger proteins with mRNA: Tristetraprolin-mediated AU-rich element-dependent mRNA degradation can occur in the absence of a poly(A) tail", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 25, 22 June 2001 (2001-06-22), pages 23144 - 23154, XP002302921, ISSN: 0021-9258 *
LAI WI.S. ET AL: "Interactions of CCCH zinc finger proteins with mRNA. Non-binding tristetraprolin mutants exert an inhibitory effect on degradation of", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 11, 15 March 2002 (2002-03-15), pages 9606 - 9613, XP002302924, ISSN: 0021-9258 *
MUKHERJEE D. ET AL.: "The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 21, no. 1-2, 15 January 2002 (2002-01-15), pages 165 - 174, XP002302925, ISSN: 0261-4189 *
XU N. ET AL.: "Modulation of the fate of cytoplasmic mRNA by AU-rich elements: Key sequence features controlling mRNA deadenylation and decay", MOLECULAR AND CELLULAR BIOLOGY, vol. 17, no. 8, 1997, pages 4611 - 4621, XP002302922, ISSN: 0270-7306 *

Also Published As

Publication number Publication date
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