WO2004078914A3 - Enzyme fluorase et son utilisation pour la synthese par fluoration - Google Patents

Enzyme fluorase et son utilisation pour la synthese par fluoration Download PDF

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Publication number
WO2004078914A3
WO2004078914A3 PCT/GB2004/000880 GB2004000880W WO2004078914A3 WO 2004078914 A3 WO2004078914 A3 WO 2004078914A3 GB 2004000880 W GB2004000880 W GB 2004000880W WO 2004078914 A3 WO2004078914 A3 WO 2004078914A3
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WO
WIPO (PCT)
Prior art keywords
fluorinase
polypeptide
enzyme
fluorination synthesis
fluorination
Prior art date
Application number
PCT/GB2004/000880
Other languages
English (en)
Other versions
WO2004078914A2 (fr
Inventor
David O'hagan
James Naismith
Christophe Schaffrath
Changjiang Dong
Jonathan Brian Spencer
Fanglu Huang
Original Assignee
Univ St Andrews
Univ Cambridge Tech
David O'hagan
James Naismith
Christophe Schaffrath
Changjiang Dong
Jonathan Brian Spencer
Fanglu Huang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ St Andrews, Univ Cambridge Tech, David O'hagan, James Naismith, Christophe Schaffrath, Changjiang Dong, Jonathan Brian Spencer, Fanglu Huang filed Critical Univ St Andrews
Publication of WO2004078914A2 publication Critical patent/WO2004078914A2/fr
Publication of WO2004078914A3 publication Critical patent/WO2004078914A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/0491—Sugars, nucleosides, nucleotides, oligonucleotides, nucleic acids, e.g. DNA, RNA, nucleic acid aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Optics & Photonics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un polypeptide présentant une activité enzymatique de la fluorase, un procédé destiné à la production de ce polypeptide et l'application de ce polypeptide. Ce polypeptide a été obtenu à partir de Streptomyces cattleya et a été cristallisé.
PCT/GB2004/000880 2003-03-04 2004-03-03 Enzyme fluorase et son utilisation pour la synthese par fluoration WO2004078914A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0304882.4A GB0304882D0 (en) 2003-03-04 2003-03-04 Polypetide
GB0304882.4 2003-03-04

Publications (2)

Publication Number Publication Date
WO2004078914A2 WO2004078914A2 (fr) 2004-09-16
WO2004078914A3 true WO2004078914A3 (fr) 2004-12-23

Family

ID=9954057

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/000880 WO2004078914A2 (fr) 2003-03-04 2004-03-03 Enzyme fluorase et son utilisation pour la synthese par fluoration

Country Status (2)

Country Link
GB (1) GB0304882D0 (fr)
WO (1) WO2004078914A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201110239D0 (en) 2011-06-17 2011-08-03 College The Labelling method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003020945A2 (fr) * 2001-09-05 2003-03-13 The University Court Of The University Of St Andrews Procede enzymatique de synthese de composes organofluores

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003020945A2 (fr) * 2001-09-05 2003-03-13 The University Court Of The University Of St Andrews Procede enzymatique de synthese de composes organofluores

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
CHERIF A ET AL: "RADIOSYNTHESIS AND BIODISTRIBUTION STUDIES OF [F-18]FLUOROADENOSINE AND [I-131]-5-IODO-2'-O-METHYL-URIDINE, FOR THE ASSESSMENT OF TUMOR PROLIFERATION RATE", PHARMACEUTICAL RESEARCH, NEW YORK, NY, US, vol. 12, no. 9, SUPPL, 5 November 1995 (1995-11-05), pages S128, XP008019780, ISSN: 0724-8741 *
DATABASE EMBL [online] 31 January 2004 (2004-01-31), HUANG F: "Streptomyces cattleya flA gene for 5'-fluoro-5'-deoxyadenosine synthase (5'FDA)", XP002288661, Database accession no. AJ581748 *
DONG CHANGJIANG ET AL: "Crystal structure and mechanism of a bacterial fluorinating enzyme.", NATURE (LONDON), vol. 427, no. 6974, 5 February 2004 (2004-02-05), pages 561 - 565, XP002288659, ISSN: 0028-0836 *
DONG CHANGJIANG ET AL: "Crystallization and X-ray diffraction of 5'-fluoro-5'-deoxyadenosine synthase, a fluorination enzyme from Streptomyces cattleya.", ACTA CRYSTALLOGRAPHICA SECTION D BIOLOGICAL CRYSTALLOGRAPHY, vol. 59, no. 12, December 2003 (2003-12-01), pages 2292 - 2293, XP008032822, ISSN: 0907-4449 *
LEHEL SZ ET AL: "SYNTHESIS OF 5'-DEOXY-5'-[18F]FLUORO-ADENOSINE BY RADIOFLUORINATION OF 5'-DEOXY-5'-HALOADENOSINE DERIVATIVES", JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, ARTICLES, ELSEVIER SEQUOIA S.A., LAUSANNE, CH, vol. 245, no. 2, 2000, pages 399 - 401, XP008019765 *
MOSS S J ET AL: "Fluoroacetaldehyde: A precursor of both fluoroacetate and 4-fluorothreonine in Streptomyces cattleya", CHEMICAL COMMUNICATIONS - CHEMCOM, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 6, no. 22, 21 November 2000 (2000-11-21), pages 2281 - 2282, XP002236667, ISSN: 1359-7345 *
MURPHY CORMAC D ET AL: "Isolation of an aldehyde dehydrogenase involved in the oxidation of fluoroacetaldehyde to fluoroacetate in Streptomyces cattleya", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 10, October 2001 (2001-10-01), pages 4919 - 4921, XP002288660, ISSN: 0099-2240 *
O'HAGAN D ET AL: "Biochemistry: biosynthesis of an organofluorine molecule", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 416, no. 6878, 21 March 2002 (2002-03-21), pages 279, XP002236535, ISSN: 0028-0836 *
O'HAGAN D ET AL: "Fluorine-containing natural products", JOURNAL OF FLUORINE CHEMISTRY, ELSEVIER SEQUOIA, LAUSANNE, CH, vol. 100, no. 1-2, December 1999 (1999-12-01), pages 127 - 133, XP004363077, ISSN: 0022-1139 *
REID K A ET AL: "BIOSYNTHESIS OF FLUORINATED SECONDARY METABOLITES BY STREPTOMYCES CATTLEYA", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 141, no. 6, June 1995 (1995-06-01), pages 1385 - 1393, XP008015651, ISSN: 1350-0872 *
SANADA M ET AL: "BIOSYNTHESIS OF FLUOROTHREONINE AND FLUOROACETIC-ACID BY THE THIENAMYCIN PRODUCER STREPTOMYCES-CATTLEYA", JOURNAL OF ANTIBIOTICS, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. TOKYO, JP, vol. 39, no. 2, 1986, pages 259 - 265, XP002236536, ISSN: 0021-8820 *
SCHAFFRATH C ET AL: "Cell-free biosynthesis of fluoroacetate and 4-fluorothreonine in Streptomyces cattleya", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, VERLAG CHEMIE. WEINHEIM, DE, vol. 41, no. 20, 18 October 2002 (2002-10-18), pages 3913 - 3915, XP002236538, ISSN: 0570-0833 *
SCHAFFRATH C ET AL: "Isolation and characterisation of 5'-fluorodeoxyadenosine synthase, a fluorination enzyme from Streptomyces cattleya", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 547, no. 1-3, 17 July 2003 (2003-07-17), pages 111 - 114, XP004438829, ISSN: 0014-5793 *
ZECHEL D L ET AL: "Enzymatic synthesis of carbon-fluorine bonds", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 123, no. 18, 9 May 2001 (2001-05-09), pages 4350 - 4351, XP002236668, ISSN: 0002-7863 *

Also Published As

Publication number Publication date
WO2004078914A2 (fr) 2004-09-16
GB0304882D0 (en) 2003-04-09

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