WO2004076625A3 - Method of identifying plant cells transformed with a gene - Google Patents

Method of identifying plant cells transformed with a gene Download PDF

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Publication number
WO2004076625A3
WO2004076625A3 PCT/US2004/004332 US2004004332W WO2004076625A3 WO 2004076625 A3 WO2004076625 A3 WO 2004076625A3 US 2004004332 W US2004004332 W US 2004004332W WO 2004076625 A3 WO2004076625 A3 WO 2004076625A3
Authority
WO
WIPO (PCT)
Prior art keywords
plant cells
transformed
transformed plant
gene
selectable marker
Prior art date
Application number
PCT/US2004/004332
Other languages
French (fr)
Other versions
WO2004076625A2 (en
Inventor
Mark Conkling
Xia Li
Kheng Cheah
Jiahua Xie
Original Assignee
Vector Tobacco Ltd
Mark Conkling
Xia Li
Kheng Cheah
Jiahua Xie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vector Tobacco Ltd, Mark Conkling, Xia Li, Kheng Cheah, Jiahua Xie filed Critical Vector Tobacco Ltd
Publication of WO2004076625A2 publication Critical patent/WO2004076625A2/en
Publication of WO2004076625A3 publication Critical patent/WO2004076625A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Several approaches to differentiate transformed plant cells from non-transformed plant cells, in the absence of a selectable marker, are aspects of the invention described herein. It was discovered that transformed plant cells experience detectable morphological changes (e.g., a reduced rate of growth or biomass) that can be readily identified and used to differentiate the transformed cell from the non-transformed cell in the absence of a selectable marker. Embodiments include approaches for differentiating, identifying, selecting, and regenerating plant cells that have been transformed with a gene of interest, with or without a selectable marker by comparing the growth rate or morphological feature of the transformed plant cells with the growth rate or a morphological feature of non-transformed plant cells that are cultured under similar conditions.
PCT/US2004/004332 2003-02-21 2004-02-13 Method of identifying plant cells transformed with a gene WO2004076625A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44950103P 2003-02-21 2003-02-21
US60/449,501 2003-02-21

Publications (2)

Publication Number Publication Date
WO2004076625A2 WO2004076625A2 (en) 2004-09-10
WO2004076625A3 true WO2004076625A3 (en) 2005-06-02

Family

ID=32927526

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/004332 WO2004076625A2 (en) 2003-02-21 2004-02-13 Method of identifying plant cells transformed with a gene

Country Status (1)

Country Link
WO (1) WO2004076625A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AP2691A (en) 2004-08-18 2013-07-16 Alellyx Sa Polynucleotides, Dna constructs and methods for the alteration of plant lignin content and/or composition
CA2926297C (en) 2005-02-28 2018-02-27 22Nd Century Limited, Llc Reducing levels of nicotinic alkaloids in plants
EP2038406A2 (en) 2006-06-19 2009-03-25 National Research Council of Canada Nucleic acid encoding n-methylputrescine oxidase and uses thereof
US9551003B2 (en) 2006-09-13 2017-01-24 22Nd Century Limited, Llc Increasing levels of nicotinic alkaloids in plants
PL2450446T3 (en) 2006-09-13 2015-10-30 22Nd Century Ltd Llc Increasing levels of nicotinic alkaloids
US9102948B2 (en) 2006-11-17 2015-08-11 22Nd Century Limited, Llc Regulating alkaloids
CA3008999C (en) 2007-05-25 2022-10-04 22Nd Century Limited, Llc Nucleic acid sequences encoding transcription factors regulating alkaloid biosynthesis and their use in modifying plant metabolism
US9253952B2 (en) 2012-08-17 2016-02-09 University Of Copenhagen Agrobacterium rhizogenes transformation and expression of rol genes in Kalanchoë
CN112836621B (en) * 2021-01-29 2022-11-08 华东理工大学青岛创新研究院 Plant cell phenotype detection method and system
CN116904495B (en) * 2023-05-26 2024-09-27 四川农业大学 Method for high-efficiency instantaneous transformation of angelica dahurica protoplasm

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743548A (en) * 1984-09-25 1988-05-10 Calgene, Inc. Plant cell microinjection technique
US5122466A (en) * 1989-06-13 1992-06-16 North Carolina State University Ballistic transformation of conifers
US5965791A (en) * 1994-11-09 1999-10-12 Nippon Paper Industries Co., Ltd. Vector for introducing a gene into a plant, and methods for producing transgenic plants and multitudinously introducing genes into a plant using the vector

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743548A (en) * 1984-09-25 1988-05-10 Calgene, Inc. Plant cell microinjection technique
US5122466A (en) * 1989-06-13 1992-06-16 North Carolina State University Ballistic transformation of conifers
US5965791A (en) * 1994-11-09 1999-10-12 Nippon Paper Industries Co., Ltd. Vector for introducing a gene into a plant, and methods for producing transgenic plants and multitudinously introducing genes into a plant using the vector

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DE BLOCK ET AL: "Expression of foreign genes in regenerated plants and in their progeny", THE EMBO JOURNAL, vol. 3, no. 8, 1984, pages 1881 - 1689, XP008045372 *
FROMM ET AL: "Stable transformation of maize transfer by electroporation", NATURE, vol. 319, February 1986 (1986-02-01), pages 791 - 793, XP002059099 *
VOELCKEL ET AL: "Anti-sense expression of putrescine N-methyltransferase conforms defensive role of nicotine in Nicotinia sylvestris against Manduca sexta", CHEMOECOLOGY, vol. 11, 2001, pages 121 - 126, XP002985493 *

Also Published As

Publication number Publication date
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