WO2004076625A3 - Method of identifying plant cells transformed with a gene - Google Patents
Method of identifying plant cells transformed with a gene Download PDFInfo
- Publication number
- WO2004076625A3 WO2004076625A3 PCT/US2004/004332 US2004004332W WO2004076625A3 WO 2004076625 A3 WO2004076625 A3 WO 2004076625A3 US 2004004332 W US2004004332 W US 2004004332W WO 2004076625 A3 WO2004076625 A3 WO 2004076625A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plant cells
- transformed
- transformed plant
- gene
- selectable marker
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1077—Pentosyltransferases (2.4.2)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Several approaches to differentiate transformed plant cells from non-transformed plant cells, in the absence of a selectable marker, are aspects of the invention described herein. It was discovered that transformed plant cells experience detectable morphological changes (e.g., a reduced rate of growth or biomass) that can be readily identified and used to differentiate the transformed cell from the non-transformed cell in the absence of a selectable marker. Embodiments include approaches for differentiating, identifying, selecting, and regenerating plant cells that have been transformed with a gene of interest, with or without a selectable marker by comparing the growth rate or morphological feature of the transformed plant cells with the growth rate or a morphological feature of non-transformed plant cells that are cultured under similar conditions.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US44950103P | 2003-02-21 | 2003-02-21 | |
| US60/449,501 | 2003-02-21 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2004076625A2 WO2004076625A2 (en) | 2004-09-10 |
| WO2004076625A3 true WO2004076625A3 (en) | 2005-06-02 |
Family
ID=32927526
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2004/004332 WO2004076625A2 (en) | 2003-02-21 | 2004-02-13 | Method of identifying plant cells transformed with a gene |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2004076625A2 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0514436C1 (en) | 2004-08-18 | 2008-06-24 | Alellyx Sa | method to change wood density |
| KR101563998B1 (en) | 2005-02-28 | 2015-10-28 | 22엔디 센츄리 리미티드, 엘엘씨 | Reducing levels of nicotinic alkaloids in plants |
| CN102083987B (en) | 2006-06-19 | 2017-04-26 | 22世纪有限责任公司 | Nucleic acid encoding N-methylputrescine oxidase and uses thereof |
| US9551003B2 (en) | 2006-09-13 | 2017-01-24 | 22Nd Century Limited, Llc | Increasing levels of nicotinic alkaloids in plants |
| ES2543907T3 (en) | 2006-09-13 | 2015-08-25 | 22Nd Century Limited, Llc | Increased levels of nicotinic alkaloids |
| US9102948B2 (en) | 2006-11-17 | 2015-08-11 | 22Nd Century Limited, Llc | Regulating alkaloids |
| CN110885835B (en) | 2007-05-25 | 2023-08-11 | 22世纪有限责任公司 | Nucleic acid sequences encoding transcription factors regulating alkaloid synthesis and their use in improving plant metabolism |
| US9253952B2 (en) | 2012-08-17 | 2016-02-09 | University Of Copenhagen | Agrobacterium rhizogenes transformation and expression of rol genes in Kalanchoë |
| CN112836621B (en) * | 2021-01-29 | 2022-11-08 | 华东理工大学青岛创新研究院 | Plant cell phenotype detection method and system |
| CN116904495A (en) * | 2023-05-26 | 2023-10-20 | 四川农业大学 | Method for high-efficiency instantaneous transformation of angelica dahurica protoplasm |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4743548A (en) * | 1984-09-25 | 1988-05-10 | Calgene, Inc. | Plant cell microinjection technique |
| US5122466A (en) * | 1989-06-13 | 1992-06-16 | North Carolina State University | Ballistic transformation of conifers |
| US5965791A (en) * | 1994-11-09 | 1999-10-12 | Nippon Paper Industries Co., Ltd. | Vector for introducing a gene into a plant, and methods for producing transgenic plants and multitudinously introducing genes into a plant using the vector |
-
2004
- 2004-02-13 WO PCT/US2004/004332 patent/WO2004076625A2/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4743548A (en) * | 1984-09-25 | 1988-05-10 | Calgene, Inc. | Plant cell microinjection technique |
| US5122466A (en) * | 1989-06-13 | 1992-06-16 | North Carolina State University | Ballistic transformation of conifers |
| US5965791A (en) * | 1994-11-09 | 1999-10-12 | Nippon Paper Industries Co., Ltd. | Vector for introducing a gene into a plant, and methods for producing transgenic plants and multitudinously introducing genes into a plant using the vector |
Non-Patent Citations (3)
| Title |
|---|
| DE BLOCK ET AL: "Expression of foreign genes in regenerated plants and in their progeny", THE EMBO JOURNAL, vol. 3, no. 8, 1984, pages 1881 - 1689, XP008045372 * |
| FROMM ET AL: "Stable transformation of maize transfer by electroporation", NATURE, vol. 319, February 1986 (1986-02-01), pages 791 - 793, XP002059099 * |
| VOELCKEL ET AL: "Anti-sense expression of putrescine N-methyltransferase conforms defensive role of nicotine in Nicotinia sylvestris against Manduca sexta", CHEMOECOLOGY, vol. 11, 2001, pages 121 - 126, XP002985493 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004076625A2 (en) | 2004-09-10 |
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