WO2004069993A3 - Non-touch zona pellucida microdrilling of a single - cell embryo with a 1.48 um laser beam for introduction of retrovirus - Google Patents

Non-touch zona pellucida microdrilling of a single - cell embryo with a 1.48 um laser beam for introduction of retrovirus Download PDF

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Publication number
WO2004069993A3
WO2004069993A3 PCT/EP2004/001101 EP2004001101W WO2004069993A3 WO 2004069993 A3 WO2004069993 A3 WO 2004069993A3 EP 2004001101 W EP2004001101 W EP 2004001101W WO 2004069993 A3 WO2004069993 A3 WO 2004069993A3
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WO
WIPO (PCT)
Prior art keywords
retrovirus
microdrilling
introduction
touch
laser beam
Prior art date
Application number
PCT/EP2004/001101
Other languages
French (fr)
Other versions
WO2004069993A2 (en
Inventor
Alfred Senn
Thierry Pedrazzini
Original Assignee
Octax Microsciences Gmbh
Mtg Medical Technology Vertrie
Univ Lausanne
Alfred Senn
Thierry Pedrazzini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Octax Microsciences Gmbh, Mtg Medical Technology Vertrie, Univ Lausanne, Alfred Senn, Thierry Pedrazzini filed Critical Octax Microsciences Gmbh
Priority to EP04708744A priority Critical patent/EP1589811A2/en
Publication of WO2004069993A2 publication Critical patent/WO2004069993A2/en
Publication of WO2004069993A3 publication Critical patent/WO2004069993A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Electromagnetism (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention concerns the use of a 1.48 µm laser to microdrill a hole in the zone pellucida of a single-cell embryo. Thereby, a retrovirus, especially a Lentivirus carrying a particular transgene can be introduced into said embryo.
PCT/EP2004/001101 2003-02-06 2004-02-06 Non-touch zona pellucida microdrilling of a single - cell embryo with a 1.48 um laser beam for introduction of retrovirus WO2004069993A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04708744A EP1589811A2 (en) 2003-02-06 2004-02-06 Non-touch zona pellucida microdrilling of a single-cell embryo with a 1.48 um diode laser beam for introduction of retrovirus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44588503P 2003-02-06 2003-02-06
US60/445,885 2003-02-06

Publications (2)

Publication Number Publication Date
WO2004069993A2 WO2004069993A2 (en) 2004-08-19
WO2004069993A3 true WO2004069993A3 (en) 2004-09-23

Family

ID=32851012

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/001101 WO2004069993A2 (en) 2003-02-06 2004-02-06 Non-touch zona pellucida microdrilling of a single - cell embryo with a 1.48 um laser beam for introduction of retrovirus

Country Status (2)

Country Link
EP (1) EP1589811A2 (en)
WO (1) WO2004069993A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017184757A1 (en) 2016-04-20 2017-10-26 Coopersurgical, Inc. Beam steering for laser systems and related methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LOIS CARLOS ET AL: "Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors", SCIENCE (WASHINGTON D C), vol. 295, no. 5556, 1 February 2002 (2002-02-01), pages 868 - 872, XP002288582, ISSN: 0036-8075 *
RINK K ET AL: "NON-CONTACT MICRODRILLING OF MOUSE ZONA PELLUCIDA WITH AN OBJECTIVE-DELIVERED 1.48-MUM DIODE LASER", LASERS IN SURGERY AND MEDICINE, WILEY- LISS, NEW YORK, US, vol. 18, no. 1, 1996, pages 52 - 62, XP009002593, ISSN: 0196-8092 *

Also Published As

Publication number Publication date
EP1589811A2 (en) 2005-11-02
WO2004069993A2 (en) 2004-08-19

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