WO2004067709A3 - Identification of essential genes of aspergillus fumigatus and methods of use - Google Patents

Identification of essential genes of aspergillus fumigatus and methods of use Download PDF

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Publication number
WO2004067709A3
WO2004067709A3 PCT/US2004/001099 US2004001099W WO2004067709A3 WO 2004067709 A3 WO2004067709 A3 WO 2004067709A3 US 2004001099 W US2004001099 W US 2004001099W WO 2004067709 A3 WO2004067709 A3 WO 2004067709A3
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WO
WIPO (PCT)
Prior art keywords
genes
essential
aspergillus fumigatus
essential genes
methods
Prior art date
Application number
PCT/US2004/001099
Other languages
French (fr)
Other versions
WO2004067709A8 (en
WO2004067709A2 (en
Inventor
Bo Jiang
Wenqi Hu
Sebastien Lemieux
Terry Roemer
Original Assignee
Elitra Pharmaceuticals Inc
Elitra Canada Ltd
Bo Jiang
Wenqi Hu
Sebastien Lemieux
Terry Roemer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elitra Pharmaceuticals Inc, Elitra Canada Ltd, Bo Jiang, Wenqi Hu, Sebastien Lemieux, Terry Roemer filed Critical Elitra Pharmaceuticals Inc
Publication of WO2004067709A2 publication Critical patent/WO2004067709A2/en
Publication of WO2004067709A3 publication Critical patent/WO2004067709A3/en
Publication of WO2004067709A8 publication Critical patent/WO2004067709A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides Aspergillus fumigatus genes that are essential and are potential targets for drug screening. The identification of essential genes and those genes critical to the development of virulent infections, provides a basis for the development of screens for new drugs against Aspergillus fumigatus. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic acid arrays. The present invention further provides methods for the construction of genetic mutants in which a target gene is placed under conditional expression. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention.
PCT/US2004/001099 2003-01-17 2004-01-16 Identification of essential genes of aspergillus fumigatus and methods of use WO2004067709A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US44128103P 2003-01-17 2003-01-17
US60/441,281 2003-01-17
US47819603P 2003-06-13 2003-06-13
US60/478,196 2003-06-13

Publications (3)

Publication Number Publication Date
WO2004067709A2 WO2004067709A2 (en) 2004-08-12
WO2004067709A3 true WO2004067709A3 (en) 2007-02-01
WO2004067709A8 WO2004067709A8 (en) 2008-04-17

Family

ID=32829770

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/001099 WO2004067709A2 (en) 2003-01-17 2004-01-16 Identification of essential genes of aspergillus fumigatus and methods of use

Country Status (1)

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WO (1) WO2004067709A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7851199B2 (en) 2005-03-18 2010-12-14 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi

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GB0404433D0 (en) * 2004-02-27 2004-03-31 F2G Ltd Transposon mutagenesis
EP1756145B1 (en) 2004-06-16 2014-06-25 DSM IP Assets B.V. Production of polypeptides by improved secretion
US7794712B2 (en) 2004-06-18 2010-09-14 Simon Fraser University Production of mutant strain of Aspergillus fumigatus, method of assay for inhibiting siderophore biosynthesis and diagnostic method for detecting likely Aspergillus fumigatus infection
EP2314668B1 (en) * 2005-04-12 2016-11-09 Danisco US Inc. Gene inactivated mutants with altered protein production
EP2078092A2 (en) 2006-09-28 2009-07-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
DE102006046719A1 (en) * 2006-10-02 2008-04-03 Ab Enzymes Gmbh DNA sequence coding for an Aspergillus fumigatus phospholipase, e.g. useful for desliming vegetable oil
GB0719367D0 (en) 2007-10-03 2007-11-14 Procarta Biosystems Ltd Transcription factor decoys, compositions and methods
EP2101176A1 (en) * 2008-03-06 2009-09-16 Technische Universität Braunschweig Diagnosis of and vaccination against Aspergillus fumigatus
CN103923992B (en) * 2008-05-28 2016-04-27 花王株式会社 The detection method of thermotolerant bacterium
GB201002413D0 (en) 2010-02-12 2010-03-31 Procarta Biosystems Ltd Nucleic acid complexes
GB201005545D0 (en) * 2010-04-01 2010-05-19 Procarta Biosystems Ltd Transcription factor decoys
EP3358000A3 (en) 2012-01-05 2018-12-19 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
WO2014059541A1 (en) * 2012-10-16 2014-04-24 Concordia University Novel cell wall deconstruction enzymes of thermoascus aurantiacus, myceliophthora fergusii (corynascus thermophilus), and pseudocercosporella herpotrichoides, and uses thereof
KR20160035587A (en) 2013-07-10 2016-03-31 노파르티스 아게 Multiple proteases deficient filamentous fungal cells and methods of use thereof
WO2016012468A1 (en) 2014-07-21 2016-01-28 Novartis Ag Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
EP3687629A4 (en) * 2017-09-27 2021-10-06 University of Georgia Research Foundation Treatment and detection of infection and disease associated with different fungal pathogens
US11667922B2 (en) 2018-03-26 2023-06-06 Novozymes A/S Fungal chaperone proteins
GB201810053D0 (en) * 2018-06-19 2018-08-01 Medizinische Univ Innsbruck Genetic selection markers based on enzymatic activities of the pyrimidine salvage pathway

Citations (2)

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US20010053519A1 (en) * 1990-12-06 2001-12-20 Fodor Stephen P.A. Oligonucleotides
US6558920B1 (en) * 1999-06-01 2003-05-06 Gekkeikan Sake Company Limited High expression system of proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010053519A1 (en) * 1990-12-06 2001-12-20 Fodor Stephen P.A. Oligonucleotides
US6558920B1 (en) * 1999-06-01 2003-05-06 Gekkeikan Sake Company Limited High expression system of proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 28 March 2003 (2003-03-28), MACHIDA M. ET AL.: "Aspergillus oryzae polynucleotide SEQ ID NO: 3164", XP003007865, Database accession no. (ABZ54051) *
NIERMAN W. ET AL.: "Genomic Sequence of the Pathogenic and Allergenic Filamentous Fungus Aspergillus Fumigatus", NATURE, vol. 438, no. 7071, December 2005 (2005-12-01), pages 1151 - 1156, XP002415975 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7851199B2 (en) 2005-03-18 2010-12-14 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi

Also Published As

Publication number Publication date
WO2004067709A8 (en) 2008-04-17
WO2004067709A2 (en) 2004-08-12

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