WO2004067480A2 - Substituted phenylurea derivatives as hdac inhibitors - Google Patents

Substituted phenylurea derivatives as hdac inhibitors Download PDF

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Publication number
WO2004067480A2
WO2004067480A2 PCT/GB2004/000238 GB2004000238W WO2004067480A2 WO 2004067480 A2 WO2004067480 A2 WO 2004067480A2 GB 2004000238 W GB2004000238 W GB 2004000238W WO 2004067480 A2 WO2004067480 A2 WO 2004067480A2
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compound
alkyl
hydrogen
ureido
formula
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PCT/GB2004/000238
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French (fr)
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WO2004067480A3 (en
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David Ian Carter Scopes
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Oxford Glycosciences (Uk) Ltd
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Priority claimed from GB0301872A external-priority patent/GB0301872D0/en
Priority claimed from GB0324655A external-priority patent/GB0324655D0/en
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Publication of WO2004067480A2 publication Critical patent/WO2004067480A2/en
Publication of WO2004067480A3 publication Critical patent/WO2004067480A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D263/00—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings
    • C07D263/52—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings condensed with carbocyclic rings or ring systems
    • C07D263/54—Benzoxazoles; Hydrogenated benzoxazoles
    • C07D263/56—Benzoxazoles; Hydrogenated benzoxazoles with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached in position 2
    • C07D263/57—Aryl or substituted aryl radicals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Definitions

  • the present invention relates to novel compounds that are useful as inhibitors of histone deacetylase. It also relates to methods for their synthesis, pharmaceutical compositions comprising the novel compounds and their use in medicine, in particular for the treatment of cancer.
  • DNA in eukaryotic cells is tightly associated with histone proteins to form nucleosomes, which represent the basic structure of chromatin.
  • the histones can be reversibly modified by a number of post- translational reactions, such as phosphorylation, methylation, ADP-ribosylation and acetylation (Davie J R & Spencer V A (1999) J. Cell. Biochem, Suppl 32-33, 141-8).
  • the dynamic state of histone acetylation is tightly regulated and maintained by two enzyme activities: histone acetyltransferases and histone deacetylases.
  • Acetylation of histones is a reversible modification that occurs during the assembly of nucleosomes and during DNA synthesis and transcription.
  • Histone hyperacetylation correlates with an open decondensed chromatin structure and gene activation, while hypoacetylation correlates with chromatin condensation and transcriptional repression (Katan-Khaykovich Y & Struhl K (2002) Genes Dev, Mar 15, 16(6), 743-52).
  • histone deacetylase catalyses the removal of the acetyl group from the free amino group of lysine residues on the N-terminal part of the nucleosome bound histone.
  • the liberated lysine residues of the histones confer a positive charge, which can associate with the negatively charged phosphate groups of the DNA.
  • This stronger association results in a tighter conformational configuration that prevents transcriptional activators binding to their DNA promoter sites and effectively deactivates gene expression (Davie J R & Spencer V A (1999) J. Cell. Biochem., Suppl 32-33, 141-8).
  • histone deacetylases will exist associated in a multi-protein complex that includes transcription repressor proteins, e.g. mSin3A, (David et al., (1998) Oncogene, 16, 2549-2556 & Ayer DE, (1999) Trends Cell Biol., May, 9(5), 193-8).
  • transcription repressor proteins e.g. mSin3A
  • histone acetylase is found complexed/ " associated with transcription activators and indeed some transcriptional activators have intrinsic histone acetylase activity (Strahl BD & Allis CD (2000) Nature, 403, 41).
  • HDAC human histone deacetylase
  • reversible histone acetylation is a major regulatory mechanism that modulates gene expression. This regulation of transcription essentially controls cellular processes such as cellular differentiation and cellular growth.
  • histone deacetylase inhibitors can reactive gene expression and inhibit the growth and survival of cancerous cells (Reviewed in Johnstone RW, 2002, Nat. Rev. Drug Discov., Apr;l(4):287-99). For example, it has been demonstrated that histone deacetylase inhibitors can induce differentiation in some myeloid leukemia cell lines (reviewed in Melnick A, Licht JD. (2002) Curr. Opin. He atol., Jul, 9(4), 322-32).
  • Histone deacetylases have also been implicated in localised angiogenesis (Kim MS et al., 2001, Nat. Med., Apr;7(4), 437-43) and cell-mediated inflammation processes (Takahashi I et al., 1996, J. Antibiot., (Tokyo) May, 49(5), 453-7). These are processes, which may contribute to skin disorders such as psoriasis and therefore, histone deacetylase inhibitors may also find use in the treatment of proliferative skin disorders, such as but not limited to, psoriasis.
  • TSA trichostatin A
  • SAHA suberoylanilide hydroxamic acid
  • phenylbutyrate and other butyrate derivatives amongst others and these are described and reviewed in Furumai R et al., 2001 Proc. Natl. Acad. Sci., U S A, Jan 2; 98(1): 87-92; Johnstone, Ricky W (2002) Nature Reviews Drug Discovery, 1(4), 287-299; Gore, Steven D.
  • the present invention provides a class of compounds, which can be used as inhibitors of histone deacetylases. These compounds provide the opportunity for establishing new treatments for cancer, angiogenesis, inflammatory and autoimmune conditions, cardiovascular diseases and proliferative disorders of the skin.
  • the invention provides a compound of formula (I) or a pharmaceutically acceptable salt or prodrug thereof:
  • R ⁇ R 2 and R 3 are independently, hydrogen, halogen, CF 3 , OR 6 , NR 7 R 8 , NR 8 COR 10 , NR 8 S0 2 R 1: or C ⁇ _ 6 alkyl optionally substituted by hydroxyl or C ⁇ _ 6 alkoxy;
  • R 4 is NR 8 CONR 8 R 9 ;
  • X and Y are R 11 and the other is hydrogen or halogen; or X and Y together with the carbon atoms to which they are attached form a fused six-membered aromatic ring;
  • Z is NR 8 , O or S;
  • R 6 is hydrogen or . 6 alkyl, C 3 . 6 alkenyl or C 3 . 6 alkynyl any of which can optionally be substituted by hydroxyl, C ⁇ . 6 alkoxy or NR 7 R 8 ;
  • R 7 is hydrogen or C ⁇ _ 6 alkyl or C 3 . 6 alkenyl either of which can optionally be substituted by . 6 alkoxy; R 8 is hydrogen or C ⁇ profession 6 alkyl; or the groups R 7 and R s may together with the nitrogen to which they are attached form a 5- or 6- membered ring which optionally contains up to two further heteroatoms selected from NR 8 , S and O;
  • R 9 is Cj.io alkyl or C 3 . ⁇ 0 alkenyl wherein a -CH 2 - group other than that adjacent to the N may be replaced by -O- and wherein the alkyl or alkenyl is substituted by one or more hydroxamic acid groups (CONHOH); or in R 4 the groups R 8 and R 9 may together with the nitrogen to which they are attached form a 5- or 6-membered ring, which is substituted with one or more hydroxamic acid groups (CONHOH);
  • R 10 is C x _ 6 alkyl
  • R 11 is hydrogen, halogen, C ⁇ profession 6 alkyl, CF 3 , OCF 3 , CN, OR 6 or phenyl optionally substituted by one or more substituents selected from halogen, C ⁇ _ 6 alkyl, CF 3 , OCF 3 , OR 6 , CN and methylenedioxo; or a 5- to 10-membered heteroaryl group containing up to three heteroatoms selected from O, N and S, which heteroaryl group may optionally be substituted by one or more substituents selected from C ⁇ profession 6 alkyl, C ⁇ _ 6 alkoxy and halogen.
  • R 1 , R 2 and R 3 are independently, hydrogen, halogen, OR 6 , NR 7 R 8 , or C . 6 alkyl optionally substituted by hydroxyl or C ⁇ _ 6 alkoxy.
  • Z is O.
  • R 6 is hydrogen or ⁇ . 6 alkyl or C 3 . 6 alkenyl either of which can optionally be substituted by hydroxyl or C ⁇ . 6 alkoxy.
  • R 9 is substituted by one hydroxamic acid group (CONHOH). More preferably R 1 is hydrogen, OR 6 or NR 7 R 8 .
  • R 2 is hydrogen
  • R 3 is hydrogen, halogen or OR 6 .
  • the ring may be, for example, morpholine, piperazine or N- methyl piperazine.
  • R 11 is a 5- to 10-membered heteroaryl group containing up to three heteroatoms selected from O, N and S
  • the group may be, for example, benzofuran e.g. 2-benzofuran, benzothiophene e.g. 2- benzothiophene, benzoxazole e.g. 2-benzoxazole, benzothiazole e.g. 2-benzothiazole, quinoline, isoquinoline, pyridine, pyrimidine, pyrazine, oxadiazole, imidazole, tetrazole, furan and thiophene.
  • R 11 is hydrogen, halogen, e.g. fluoro, C ⁇ _ 6 alkyl, CF 3 , OCF 3 , CN, OR 6 , e.g. methoxy, or phenyl.
  • the configuration of the R groups is preferably,
  • alkyl as used herein whether on its own or as part of a larger group e.g. "alkoxy” includes both straight and branched chain radicals.
  • alkyl also includes those radicals wherein one or more hydrogen atoms are replaced by fluorine, e.g. CF 3 .
  • alkenyl and alkynyl as used herein includes both straight and branched chain radicals.
  • a group of compounds of the invention which may be mentioned are those of formula (la) and pharmaceutically acceptable salts and prodrugs thereof:
  • R 2 and R 3 are independently, hydrogen, halogen, CF 3 , OR 6 , NR 7 R 8 , NR 8 COR 10 , NR 8 S0 2 R 10 or C ⁇ - 6 alkyl optionally substituted by hydroxyl or Q. 6 alkoxy;
  • R 4 is NR 8 CONR 8 R 9 ;
  • R 5 is
  • X and Y is R 11 and the othe is hydrogen or halogen; or X and Y together with the carbon atoms to which they are attached form a fused six-membered aromatic ring; Z is NR 8 , O or S;
  • R 6 is hydrogen or Q_ 6 alkyl, C 3 _ 6 alkenyl or C 3 . 6 alkynyl any of which can optionally be substituted by hydroxyl, Q_ 6 alkoxy or NR 7 R 8 ;
  • R 7 is hydrogen or Q_ 6 alkyl or C 3 . 6 alkenyl either of which can optionally be substituted by Q_ 6 alkoxy;
  • R 8 is hydrogen or Q_ 6 alkyl; or the groups R 7 and R 8 may together with the nitrogen to which they are attached form a 5- or 6- membered ring which optionally contains up to two further heteroatoms selected from NR 8 , S and O;
  • R 9 is C ⁇ _ ⁇ o alkyl or C 3 _ ⁇ o alkenyl wherein a -CH 2 - group other than that adjacent to the N may be replaced by -O- and wherein the alkyl or alkenyl is substituted by one or more hydroxamic acid groups (CONHOH); or in R 4 the groups R 8 and R 9 may together with the nitrogen to which they are attached form a 5- or 6-membered ring, which is substituted with one or more hydroxamic acid groups (CONHOH);
  • R 10 is C ⁇ -6 alkyl
  • R ⁇ is hydrogen, halogen, Q_ 6 alkyl, OR 6 or phenyl optionally substituted by one or more substituents selected from halogen, Q_ 6 alkyl, CF 3 , OCF 3 , OR 6 , CN and methylenedioxo; or a 5- to 10- membered heteroaryl group containing up to three heteroatoms selected from O, N and S, which heteroaryl group may optionally be substituted by one or more substituents selected from . 6 alkyl, Q. 6 alkoxy and halogen.
  • Preferred specific compounds of the invention include those described in the Examples.
  • references to compounds of formula (I) encompasses the pharmaceutically acceptable salts and prodrugs thereof.
  • Suitable, pharmaceutically acceptable salts of the compounds of formula (I) include, but are not limited to, salts with inorganic acids such as hydrochloride, sulfate, phosphate, diphosphate, hydrobromide, and nitrate, or salts with an organic acid such as malate, maleate, fumarate, tartrate, succinate, citrate, acetate, lactate, methanesulfonate, p-toluenesulfonate, palmitate, salicylate, and stearate. Salts may be prepared in a conventional manner using methods well known in the art.
  • the invention also includes prodrugs of the aforementioned compounds.
  • a prodrug is commonly described as an inactive or protected derivative of an active ingredient or a drug, which is converted to the active ingredient or drug in the body.
  • the invention extends to active derivatives of the aforementioned compounds.
  • Some of the compounds of this invention may be crystallised or recrystallised from solvents such as aqueous and organic solvents. In such cases solvates may be formed.
  • This invention includes within its scope stoichiometric solvates including hydrates as well as compounds containing variable amounts of water that may be produced by processes such as lyophilisation.
  • Certain of the compounds of formula (1) may exist in the form of optical isomers, e.g. diastereoisomers and mixtures of isomers in all ratios, e.g. racemic mixtures. The invention includes all such forms, in particular the pure isomeric forms.
  • the different isomeric forms may be separated or resolved one from the other by conventional methods, or any given isomer may be obtained by conventional synthetic methods or by stereospecific or asymmetric syntheses.
  • a compound contains an alkene moiety, the alkene can be presented as a cis or trans isomer or a mixture thereof.
  • an isomeric form of a compound of the invention is provided substantially free of other isomers, it will preferably contain less than 5% w/w, more preferably less than 2% w/w and especially less than 1% w/w of the other isomers.
  • the compounds of formula (I) are intended for use in pharmaceutical compositions it will readily be understood that they are each preferably provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure and preferably at least 85%, especially at least 98% pure (% are on a weight for weight basis). Impure preparations of the compounds may be used for preparing the more pure forms used in the pharmaceutical compositions; these less pure preparations of the compounds should contain at least 1%, more suitably at least 5% and preferably at least 10% of a compound of the formula (I) or pharmaceutically acceptable derivative thereof.
  • the compounds of formula (I) can be prepared by art-recognized procedures from known or commercially available starting materials. If the starting materials are unavailable from a commercial source, their synthesis is described herein, or they can be prepared by procedures known in the art.
  • the invention also provides a process for preparing a compound of formula (I) from a corresponding protected oxyamide intermediate compound, i.e. a compound as defined in formula (I), wherein the one or more hydroxamic acid groups (CONHOH) are replaced by CONHOP wherein P is a protecting group, for example, benzyloxyamide or tert-butyldimethylsilyloxyamide, by hydrogenation e.g.
  • the acid mediated removal of the tert-butyldimethylsilyl group can be performed as a separate step or can be performed as part of the work-up procedure.
  • the protected oxyamide intermediate compound may be prepared by conversion of the corresponding free acid of formula (II):
  • R 1 , R 2 , R 3 and R 5 are as defined for formula (I) and R 4' is NR 8 CONR 8 R A where R 8 is as defined for formula (I) and R ⁇ is Q_ ⁇ 0 alkyl or C 3 _ ⁇ 0 alkenyl wherein a -CH 2 - group other than that adjacent to the N may be replaced by -O- and wherein the alkyl or alkenyl is substituted with one or more carboxylic acid groups; by coupling with a protected hydroxylamine, for example, benzylhydroxylamine or te/-t- butyldimethylsilylhydroxylamine in THF under an argon atmosphere.
  • An ester compound of formula (II) can be converted to a free acid compound of formula (H) by hydrolysis, using methods well known to those skilled in the art.
  • a compound of formula (II) may be prepared from a compound of formula (III):
  • a compound of formula (III) where R x is NH 2 may be prepared from a corresponding compound where R x is N0 2 by methods well known to those skilled in the art, for example, hydrogenation with palladium on a charcoal catalyst or treatment with Zn and acetic acid.
  • a compound of formula (III) wherein R x is NH 2 may be converted to a compound of formula (III) wherein R x is NCO by reaction with, for example, triphosgene.
  • a compound of formula (111) wherein R x is NH 2 may be converted to another compound of formula (HI) wherein R x is NHR 8 and R 8 is Q. 6 alkyl, by an alkylation or reductive amination reaction using methods well known to those skilled in the art.
  • a process for preparing a compound of formula (II), comprises: treating a compound of formula (III) wherein R x is NHR 8 , with a compound of formula (IN):
  • R A is Q_ I0 alkyl or C 3 _ ⁇ 0 alkenyl wherein a -CH 2 - group other than that adjacent to the ⁇ may be replaced by -O- and wherein the alkyl or alkenyl is substituted with one or more carboxylate ester groups, e.g. by stirring at room temperature or with heating in a suitable solvent, for example THF.
  • a compound of formula (III) wherein R x is ⁇ CO is treated with a compound of formula (N):
  • the compounds of formula (III) wherein R x is ⁇ 0 2 , R 1 , R 2 , R 3 and R 5 are as defined for formula (I) and Z is O or S, may be prepared by treatment of a compound of formula (VI) wherein R 1 , R 2 and R 3 are as defined for formula (I) and R B is C0 2 H or CHO:
  • (VII) by e.g. either (i) heating in a condensation/cyclisation reaction, using for example polyphosphoric acid, or (ii) by firstly coupling a compound of formula (VI) to a compound of formula (NH) via either an ester/ thioester or amide formation reaction using methods well known to those of skill in the art followed by direct heating or heating with an acidic media with a suitable solvent to effect cyclisation, for example, p- toluenesulfonic acid in toluene.
  • this may be achieved via oxidative cyclisation of a Schiff base, derived from the condensation of the 2-aminophenol or 2-aminothiophenol and aldehydes, using various oxidants such as PhI(OAc) 2 , Pb(OAc) or DDQ.
  • R B in compound (NI) is CHO, heating in acetonitrile followed by oxidation using for example 0 2 FeCl 3 (cat.) in acetonitrile.
  • Compounds of formula (NIH) may be prepared by reduction of compounds of formula (IX) wherein X, Y and R 8 are as defined for formula (I) by methods well known to those skilled in the art, for example, hydrogenation with palladium on a charcoal catalyst or treatment with Zn and acetic acid:
  • the compounds of formula (III), wherein X or Y is halogen, can be modified to give a corresponding set of compounds of formula (IH) wherein X or Y is phenyl or a 5- to 10-membered heteroaryl group either of which is optionally substituted by one or more substituents as defined in formula (I).
  • the modification may be achieved by a coupling reaction with compounds of formula (XI) using an appropriate catalyst for example tetrakis (triphenylphosphine) palladium: R c ⁇ B(OH) 2
  • R c is phenyl or a 5- to 10-membered heteroaryl group either of which is optionally substituted by one or more substituents as defined for formula (I).
  • compounds of formula (111) where R x is N0 2 , R 3 is halogen at a position ortho or para to the R x group and R 1 , R 2 and R 5 are as defined for formula (I) may be converted to a corresponding subset of compounds of formula (HI) where R 3 is OR 6 or NR 7 R 8 , by reaction with an alcohol or amine via a nucleophilic aromatic substitution.
  • Compounds of formula (III) where R 3 is NR 8 COR 10 or NR 8 S0 2 R 10 may be prepared from corresponding compounds of formula (III) where R 3 is NHR 8 by reaction with the appropriate carboxylic acid/chloride or sulfonyl chloride, i.e. R 10 CO 2 H/(R 10 CO 2 Cl) or R 10 SO 2 Cl wherein R 10 is as defined for formula (1).
  • labile functional groups in the intermediate compounds e.g. hydroxy, carboxy and amino groups
  • the protecting groups may be removed at any stage in the synthesis of the compounds of formula (1) or may be present on the final compound of formula (I).
  • a comprehensive discussion of the ways in which various labile functional groups may be protected and methods for cleaving the resulting protected derivatives is given in for example Protective Groups in Organic Chemistry, T.W. Greene and P.G.M. Wuts (Wiley-Interscience, New York, 2nd edition, 1991).
  • the compounds of formula (I) may be prepared singly or as compound libraries comprising at least 2, for example 5 to 1,000 compounds, and more preferably 10 to 100 compounds of formula (I).
  • Libraries of compounds of formula (I) may be prepared by multiple parallel synthesis using either solution phase or solid phase chemistry, by procedures known to those skilled in the art.
  • a compound library comprising at least 2 compounds of formula (I) or pharmaceutically acceptable salts and prodrugs thereof.
  • the pharmaceutically acceptable salts and prodrugs of the compounds of formula (I) may be prepared by methods well known to those skilled in the art. Any novel intermediate compounds as described herein also fall within the scope of the present invention, fn particular the invention provides a compound as defined by formula (I) wherein the one or more hydroxamic acid groups (CONHOH) are replaced by CONHOP wherein P is a protecting group e.g. benzyloxyamide, tert-butyldimethylsilyloxyamide or tert-butyldiphenylsilyloxyamide.
  • P is a protecting group e.g. benzyloxyamide, tert-butyldimethylsilyloxyamide or tert-butyldiphenylsilyloxyamide.
  • Preferred intermediate compounds include those described in the examples.
  • the pharmaceutically effective compounds of formula (I) may be administered in conventional dosage forms prepared by combining a compound of formula (I) ("active ingredient") with standard pharmaceutical carriers or excipients according to conventional procedures well known in the art. These procedures may involve mixing, granulating and compressing or dissolving the ingredients as appropriate to the desired preparation. According to a further aspect of the invention there is provided a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt or prodrug thereof, together with one or more pharmaceutically acceptable carriers or excipients.
  • the active ingredient or pharmaceutical composition can be administered simultaneously, separately or sequentially with another appropriate treatment for cancer, angiogenesis, inflammatory and autoimmune conditions, cardiovascular diseases and proliferative disorders of the skin.
  • the active ingredient or pharmaceutical composition may be administered to a subject by any of the routes conventionally used for drug administration, for example they may be adapted for oral (including buccal, sublingual), topical (including txansdermal), nasal (including inhalation), rectal, vaginal or parenteral (including subcutaneous, intramuscular, intravenous or intradermal) administration to mammals including humans.
  • the most suitable route for administration in any given case will depend on the particular compound or pharmaceutical composition, the subject, and the nature and severity of the disease and the physical condition of the subject.
  • compositions may be prepared by any method known in the art of pharmacy, for example by bringing into association the active ingredient with the carrier(s) or excipient(s).
  • Pharmaceutical compositions adapted for oral administration may be presented as discrete units such as capsules or tablets; powders or granules; solutions or suspensions in aqueous or non-aqueous liquids; edible foams or whips; or oil-in-water liquid emulsions or water-in-oil liquid emulsions.
  • Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants, for example potato starch; or acceptable wetting agents such as sodium lauryl sulfate.
  • the tablets may be coated according to methods well known in normal pharmaceutical practice.
  • Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use.
  • Such liquid preparations may contain conventional additives, such as suspending agents, for example sorbitol, methyl cellulose, glucose syrup, gelatin, hydroxyethyl cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl ⁇ -hydroxybenzoate or sorbic acid, and, if desired, conventional flavouring or colouring agents.
  • suspending agents for example sorbitol, methyl cellulose, glucose syrup, gelatin, hydroxyethyl cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agents, for example lecithin, sorbitan monooleate, or
  • compositions adapted for topical administration may be formulated as ointments, creams, suspensions, lotions, powders, solutions, pastes, gels, impregnated dressings, sprays, aerosols or oils and may contain appropriate conventional additives such as preservatives, solvents to assist drug penetration and emollients in ointments and creams.
  • Such applications include those to the eye or other external tissues, for example the mouth and skin and the compositions are preferably applied as a topical ointment or cream.
  • the active ingredient may be employed with either a paraffinic or a water- miscible ointment base.
  • the active ingredient may be formulated in a cream with an oil-in- water cream base or a water-in-oil base.
  • the composition may also contain compatible conventional carriers, such as cream or ointment bases and ethanol or oleyl alcohol for lotions.
  • compositions adapted for topical administration to the eye include eye drops wherein the active ingredient is dissolved or suspended in a suitable carrier, especially an aqueous solvent.
  • compositions adapted for topical administration in the mouth include lozenges, pastilles and mouth washes.
  • compositions adapted for transdermal administration may be presented as discrete patches intended to remain in intimate contact with the epidermis of the recipient for a prolonged period of time.
  • the active ingredient may be delivered from the patch by iontophoresis as generally described in Pharmaceutical Research, 3(6), 318 (1986).
  • Pharmaceutical compositions adapted for nasal administration wherein the carrier is a solid include a coarse powder having a particle size for example in the range 20 to 500 microns which is administered by rapid inhalation through the nasal passage from a container of the powder held close up to the nose.
  • Suitable compositions wherein the carrier is a liquid, for administration as a nasal spray or as nasal drops include aqueous or oil solutions of the active ingredient.
  • compositions adapted for administration by inhalation include fine particle dusts or mists which may be generated by means of various types of metered dose pressurised aerosols, nebulizers or insufflators.
  • compositions adapted for rectal administration may be presented as suppositories or enemas. Suppositories will contain conventional suppository bases, e.g. cocoa-butter or other glyceride. Pharmaceutical compositions adapted for vaginal administration may be presented as pessaries, tampons, creams, gels, pastes, foams or spray compositions.
  • compositions adapted for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents.
  • the compositions may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example water for injections, immediately prior to use.
  • Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets.
  • fluid unit dosage forms are prepared utilizing the active ingredient and a sterile vehicle, water being preferred.
  • the active ingredient depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle.
  • the active ingredient can be dissolved in water for injection and filter sterilised before filling into a suitable vial or ampoule and sealing.
  • agents such as a local anaesthetic, preservative and buffering agents can be dissolved in the vehicle.
  • the composition can be frozen after filling into the vial and the water removed under vacuum.
  • the dry lyophilized powder is then sealed in the vial and an accompanying vial of water for injection may be supplied to reconstitute the liquid prior to use.
  • Parenteral suspensions are prepared in substantially the same manner except that the active ingredient is suspended in the vehicle instead of being dissolved and sterilization cannot be accomplished by filtration.
  • the active ingredient can be sterilised by exposure to ethylene oxide before suspending in the sterile vehicle.
  • a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the active ingredient.
  • the pharmaceutical compositions according to the invention are preferably adapted for oral administration.
  • compositions may also include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents. They may also contain therapeutically active agents in addition to the compounds of the present invention. Such carriers may be present as from about 1% up to about 98% of the formulation. More usually they will form up to about 80%) of the formulation.
  • compositions may contain from 0.1% by weight, preferably from 10-60% by weight, of the active material, depending on the method of administration.
  • Pharmaceutical compositions may be presented in unit dose forms containing a predetermined amount of active ingredient per dose. Such a unit may contain for example 0. lmg/kg to 750mg/kg, more preferably 0. lmg/kg to lOmg/kg depending on the condition being treated, the route of a ⁇ teinistration and the age, weight and condition of the patient.
  • Preferred unit dosage compositions are those containing a daily dose or sub-dose, as herein above recited, or an appropriate fraction thereof, of an active ingredient.
  • the optimal quantity and spacing of individual dosages of compounds in the first and second aspects of the invention will be determined by the nature and extent of the condition being treated, the form, route and site of administration, and the particular subject being treated, and that such optimums can be determined by conventional techniques. It will also be appreciated by one of skill in the art that the optimal course of treatment, i.e., the number of doses of • the aforementioned compounds given per day for a defined number of days, can be ascertained by those skilled in the art using conventional course of treatment determination tests.
  • the compounds of the present invention are useful in that they are capable of inhibiting histone deacetylase activity.
  • the compounds can be used in the treatment of cancer.
  • the compounds of the present invention can also be used in combination with one or more additional treatments or therapeutic compounds for cancer.
  • treatments include, surgery and radiation therapy.
  • therapeutic compounds include but are not limited to cisplatin, cyclophosphamide, methotrexate, 5-fluorouracil, paclitaxel, docetaxel, vincristine, vinblastine, vinorelbine, doxorubicin, tamoxifen, toremifene, megestrol acetate, anastrozole, goserelin, anti-HER2 monoclonal antibody, capecitabine and raloxifene hydrochloride.
  • the compounds of the present invention can also be used in the treatment of angiogenesis and angiogenesis dependent diseases, which include angiogenesis associated with the growth of solid tumours and retinopathy.
  • the compounds of the present invention can also be used in combination with one or more additional treatments or therapeutic compounds for angiogenesis.
  • additional treatments or therapeutic compounds for angiogenesis include, but are not limited to, recombinant platelet-derived growth factor-BB (RegranexTM).
  • the compounds of the present invention can also be used in the treatment of inflammatory conditions, including, but not limited to, rheumatoid arthritis, inflammatory bowel disease, and wound healing.
  • the compounds of the present invention can also be used in the treatment of proliferative skin disorders, such as, but not limited to psoriasis.
  • cancer or 'carcinoma' is a malignant new growth that arises from epithelium, found in skin or, more commonly, the lining of body organs. Carcinomas tend to infiltrate into adjacent tissues and spread (metastasise) to distant organs, for example to bone, liver lung or the brain.
  • cancer includes both metastatic tumour cells and tissue and examples include, but are not limited to, melanoma, mesothelioma, lymphoma, leukaemia, fibrosarcoma, rhabdomyosarcoma, mastocytoma and the following tissue -carcinomas: colorectal, colon, prostate, lung, breast, pancreatic, intestinal, renal, gastric, bladder, ovarian, uterine, cervical, hepatic and stomach.
  • tissue -carcinomas colorectal, colon, prostate, lung, breast, pancreatic, intestinal, renal, gastric, bladder, ovarian, uterine, cervical, hepatic and stomach.
  • the present invention provides:
  • (v) a method for the treatment of cancer, which comprises the step of administering to a patient an effective amount of a compound of formula (1) .
  • a method for the treatment of inflammatory diseases such as but not limited to rheumatoid arthritis, inflammatory bowel disease, and wound healing which comprises the step of administering to a patient an effective amount of a compound of formula (I).
  • Example 2d Prepared by the method of Example 2d) from 6-[3-[2-methoxy-5-(benzoxazole-2-yl)phenyl]-3- (methyl)ureido]hexanoic acid ethyl ester (185mg, 0.42mmol) and lithium hydroxide (50mg, 2.1 lmmol) the subtitle compound was obtained, (141mg, 81%).
  • Example 2e Prepared by the method of Example 2e) from 6-[3-[5-(5-fluorobenzoxazole-2-yl)-2- methoxyphenyl]ureido]hexanoic acid (230mg, 0.55mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (165mg, 0.94mmol), N-methylmorpholine (0.18ml, 1.66mmol) and tert-butyldimethylsilyl hydroxylamine (122mg, 0.83mmol) the title compound was obtained (130mg, 54%).
  • Example 2d Prepared by the method of Example 2d) from 6-[3-[2-methoxy-5-(5-methoxybenzoxazole-2- yl)phenyl]ureido]hexanoic acid ethyl ester (42mg, 0.09mmol) and lithium hydroxide (1 lmg, 0.46mmol) the subtitle compound was obtained, (34mg, 85%).
  • a histone deacetylase assay kit was purchased from BIOMOL ® Research Laboratories, AK-500, and the manufacturers protocol was followed.
  • the assay is based upon a substrate, which has an acetylated lysine chain. Deacetylation of the substrate, by histone deacetylase enzyme, sensitises the substrate to the developer producing a fiuorophore that can be measured in a fiuorometric plate reader.
  • the assays were performed according to the BIOMOL ® protocol and the source of the histone deacetylases was HeLa nuclear extract. Components added to the wells were as follows, assay buffer (final assay volume 50 ⁇ l), inhibitors (lO ⁇ l), histone deacetylase source (HeLa extract, 15 ⁇ l containing ⁇ 4 ⁇ g), substrate (25 ⁇ l, 1/200 dilution stock). The wells were mixed and the assay incubated for 30 min at R.T. before taking readings using a Nictor2 fiuorometric plate reader (excitation at 350nM and emission detected at 440nm).
  • the compound of Example 1 was assessed for its effect on cancerous cell growth.
  • Cell cycle analysis was performed on HL60 (human promyelocytic leukaemia cell line) cells by using BrdU incorporation and flow cytometry, methods that are well known to those skilled in the art.
  • the compound of Example 1 was found to cause a dose dependant accumulation of cells in the G2M phase of the cell cycle, thus arresting the cells prior to mitosis.
  • Acetylation of histones by the compound of Example 1 was investigated using the methods described in Richon et al., (2000), PNAS, vol 98, No 18. This method is based on assessment of acetylated histone protein using a rabbit anti-acetylated histone H4 antibody and Western Blotting techniques, methods that are well known to those skilled in the art.
  • the compound of Example 1 was found to cause a dose dependant increase in histone acetylation in MCF7 (human mammary epithelial cell line) cells.

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Abstract

A series of phenylurea derivatives, further substituted on the phenyl ring by a benzoxazole, benzothiazole or benzimidazole moiety, being inhibitors of histone deacetylase, are accordingly of use in medicine, in particular for the treatment of cancer.

Description

SUBSTITUTED PHENYLUREA DERIVATIVES AS HDAC INHIBITORS
The present invention relates to novel compounds that are useful as inhibitors of histone deacetylase. It also relates to methods for their synthesis, pharmaceutical compositions comprising the novel compounds and their use in medicine, in particular for the treatment of cancer.
DNA in eukaryotic cells is tightly associated with histone proteins to form nucleosomes, which represent the basic structure of chromatin. The histones can be reversibly modified by a number of post- translational reactions, such as phosphorylation, methylation, ADP-ribosylation and acetylation (Davie J R & Spencer V A (1999) J. Cell. Biochem, Suppl 32-33, 141-8). The dynamic state of histone acetylation is tightly regulated and maintained by two enzyme activities: histone acetyltransferases and histone deacetylases. Acetylation of histones is a reversible modification that occurs during the assembly of nucleosomes and during DNA synthesis and transcription. Histone hyperacetylation correlates with an open decondensed chromatin structure and gene activation, while hypoacetylation correlates with chromatin condensation and transcriptional repression (Katan-Khaykovich Y & Struhl K (2002) Genes Dev, Mar 15, 16(6), 743-52).
The enzyme, histone deacetylase catalyses the removal of the acetyl group from the free amino group of lysine residues on the N-terminal part of the nucleosome bound histone. Thus at physiological pH, the liberated lysine residues of the histones confer a positive charge, which can associate with the negatively charged phosphate groups of the DNA. This stronger association results in a tighter conformational configuration that prevents transcriptional activators binding to their DNA promoter sites and effectively deactivates gene expression (Davie J R & Spencer V A (1999) J. Cell. Biochem., Suppl 32-33, 141-8). Commonly histone deacetylases will exist associated in a multi-protein complex that includes transcription repressor proteins, e.g. mSin3A, (David et al., (1998) Oncogene, 16, 2549-2556 & Ayer DE, (1999) Trends Cell Biol., May, 9(5), 193-8). Conversely, the opposing enzyme, histone acetylase, is found complexed/ "associated with transcription activators and indeed some transcriptional activators have intrinsic histone acetylase activity (Strahl BD & Allis CD (2000) Nature, 403, 41).
Several human histone deacetylase (HDAC) enzymes have been identified and classified based on their similarity to known yeast factors. They are grouped into three classes as follows: Class I HDACs are similar to the yeast transcriptional repressor yRPD3, while class II HDACs are related to yeast yHDAl and class III HDACs to yeast Sir2 homologue ySIR2.
Thus, reversible histone acetylation is a major regulatory mechanism that modulates gene expression. This regulation of transcription essentially controls cellular processes such as cellular differentiation and cellular growth.
Aberrant activity of either histone acetylase or histone deacetylase is a key event in the onset and progression of cancer. Thus inhibition of histone deacetylase activity in malignant cells can result in the reversal of the malignant phenotype via the re-expression of previously 'repressed' or 'silenced' genes. Previous research has shown that histone deacetylase inhibitors can reactive gene expression and inhibit the growth and survival of cancerous cells (Reviewed in Johnstone RW, 2002, Nat. Rev. Drug Discov., Apr;l(4):287-99). For example, it has been demonstrated that histone deacetylase inhibitors can induce differentiation in some myeloid leukemia cell lines (reviewed in Melnick A, Licht JD. (2002) Curr. Opin. He atol., Jul, 9(4), 322-32).
Histone deacetylases have also been implicated in localised angiogenesis (Kim MS et al., 2001, Nat. Med., Apr;7(4), 437-43) and cell-mediated inflammation processes (Takahashi I et al., 1996, J. Antibiot., (Tokyo) May, 49(5), 453-7). These are processes, which may contribute to skin disorders such as psoriasis and therefore, histone deacetylase inhibitors may also find use in the treatment of proliferative skin disorders, such as but not limited to, psoriasis.
Several classes of compounds have been identified that inhibit histone deacetylase activity e.g. trichostatin A (TSA), trapoxin, suberoylanilide hydroxamic acid (SAHA), phenylbutyrate and other butyrate derivatives amongst others and these are described and reviewed in Furumai R et al., 2001 Proc. Natl. Acad. Sci., U S A, Jan 2; 98(1): 87-92; Johnstone, Ricky W (2002) Nature Reviews Drug Discovery, 1(4), 287-299; Gore, Steven D. & Carducci, Michael A, Expert Opinion on Investigational Drugs, (2000), 9(12), 2923-2934, Yoshida M et al., (2001) Cancer Chemother. Pharmacol., Aug;48 Suppl LS20-6); WO02/50285 and WO02/26696.
However, there remains a need for novel histone deacetylase inhibitors for use as therapeutic agents in the treatment of cancer and other proliferative disorders, such as psoriasis. In particular the need is for compounds that are potent in vivo and yet exhibit minimal or controllable side-effects.
The present invention provides a class of compounds, which can be used as inhibitors of histone deacetylases. These compounds provide the opportunity for establishing new treatments for cancer, angiogenesis, inflammatory and autoimmune conditions, cardiovascular diseases and proliferative disorders of the skin.
The invention provides a compound of formula (I) or a pharmaceutically acceptable salt or prodrug thereof:
Figure imgf000003_0001
(I) wherein
R\ R2 and R3 are independently, hydrogen, halogen, CF3, OR6, NR7R8, NR8COR10, NR8S02R1: or Cι_6 alkyl optionally substituted by hydroxyl or Cι_6 alkoxy; R4 is NR8CONR8R9;
R5 is
Figure imgf000003_0002
wherein one of X and Y is R11 and the other is hydrogen or halogen; or X and Y together with the carbon atoms to which they are attached form a fused six-membered aromatic ring; Z is NR8, O or S; R6 is hydrogen or .6 alkyl, C3.6 alkenyl or C3.6 alkynyl any of which can optionally be substituted by hydroxyl, Cι.6 alkoxy or NR7R8;
R7 is hydrogen or Cι_6 alkyl or C3.6 alkenyl either of which can optionally be substituted by .6 alkoxy; R8 is hydrogen or Cι„6 alkyl; or the groups R7 and Rs may together with the nitrogen to which they are attached form a 5- or 6- membered ring which optionally contains up to two further heteroatoms selected from NR8, S and O;
R9 is Cj.io alkyl or C3.ι0 alkenyl wherein a -CH2- group other than that adjacent to the N may be replaced by -O- and wherein the alkyl or alkenyl is substituted by one or more hydroxamic acid groups (CONHOH); or in R4 the groups R8 and R9 may together with the nitrogen to which they are attached form a 5- or 6-membered ring, which is substituted with one or more hydroxamic acid groups (CONHOH);
R10 is Cx_6 alkyl; and
R11 is hydrogen, halogen, Cι„6 alkyl, CF3, OCF3, CN, OR6 or phenyl optionally substituted by one or more substituents selected from halogen, Cι_6 alkyl, CF3, OCF3, OR6, CN and methylenedioxo; or a 5- to 10-membered heteroaryl group containing up to three heteroatoms selected from O, N and S, which heteroaryl group may optionally be substituted by one or more substituents selected from Cι„6 alkyl, Cι_6 alkoxy and halogen.
Preferably R1, R2 and R3 are independently, hydrogen, halogen, OR6, NR7R8, or C .6 alkyl optionally substituted by hydroxyl or Cι_6 alkoxy.
Preferably Z is O.
Preferably R6 is hydrogen or \.6 alkyl or C3.6 alkenyl either of which can optionally be substituted by hydroxyl or Cγ.6 alkoxy.
Preferably R9 is substituted by one hydroxamic acid group (CONHOH). More preferably R1 is hydrogen, OR6 or NR7R8.
More preferably R2 is hydrogen.
More preferably R3 is hydrogen, halogen or OR6.
In the group NR7R8, when the R7 and R8 substituents, together with the nitrogen to which they are attached form a 5- or 6- membered ring, the ring may be, for example, morpholine, piperazine or N- methyl piperazine.
When R11 is a 5- to 10-membered heteroaryl group containing up to three heteroatoms selected from O, N and S, the group may be, for example, benzofuran e.g. 2-benzofuran, benzothiophene e.g. 2- benzothiophene, benzoxazole e.g. 2-benzoxazole, benzothiazole e.g. 2-benzothiazole, quinoline, isoquinoline, pyridine, pyrimidine, pyrazine, oxadiazole, imidazole, tetrazole, furan and thiophene. A specific group of compounds which may be mentioned are those where R11 is hydrogen, halogen, e.g. fluoro, Cι_6 alkyl, CF3, OCF3, CN, OR6, e.g. methoxy, or phenyl.
The configuration of the R groups is preferably,
Figure imgf000004_0001
The term "alkyl" as used herein whether on its own or as part of a larger group e.g. "alkoxy" includes both straight and branched chain radicals. The term alkyl also includes those radicals wherein one or more hydrogen atoms are replaced by fluorine, e.g. CF3.
The term "alkenyl" and "alkynyl" as used herein includes both straight and branched chain radicals. A group of compounds of the invention which may be mentioned are those of formula (la) and pharmaceutically acceptable salts and prodrugs thereof:
Figure imgf000004_0002
(I) wherein
R1. R2 and R3 are independently, hydrogen, halogen, CF3, OR6, NR7R8, NR8COR10, NR8S02R10 or Cι-6 alkyl optionally substituted by hydroxyl or Q.6 alkoxy; R4 is NR8CONR8R9; R5 is
Figure imgf000005_0001
wherein one of X and Y is R11 and the othe is hydrogen or halogen; or X and Y together with the carbon atoms to which they are attached form a fused six-membered aromatic ring; Z is NR8, O or S;
R6 is hydrogen or Q_6 alkyl, C3_6 alkenyl or C3.6 alkynyl any of which can optionally be substituted by hydroxyl, Q_6 alkoxy or NR7R8;
R7 is hydrogen or Q_6 alkyl or C3.6 alkenyl either of which can optionally be substituted by Q_6 alkoxy;
R8 is hydrogen or Q_6 alkyl; or the groups R7 and R8 may together with the nitrogen to which they are attached form a 5- or 6- membered ring which optionally contains up to two further heteroatoms selected from NR8, S and O;
R9 is Cι_ιo alkyl or C3_ιo alkenyl wherein a -CH2- group other than that adjacent to the N may be replaced by -O- and wherein the alkyl or alkenyl is substituted by one or more hydroxamic acid groups (CONHOH); or in R4 the groups R8 and R9 may together with the nitrogen to which they are attached form a 5- or 6-membered ring, which is substituted with one or more hydroxamic acid groups (CONHOH);
R10 is Cι-6 alkyl; and
Rπ is hydrogen, halogen, Q_6 alkyl, OR6 or phenyl optionally substituted by one or more substituents selected from halogen, Q_6 alkyl, CF3, OCF3, OR6, CN and methylenedioxo; or a 5- to 10- membered heteroaryl group containing up to three heteroatoms selected from O, N and S, which heteroaryl group may optionally be substituted by one or more substituents selected from .6 alkyl, Q.6 alkoxy and halogen.
Preferred specific compounds of the invention include those described in the Examples.
As described herein, for all aspects of the invention, reference to compounds of formula (I) encompasses the pharmaceutically acceptable salts and prodrugs thereof. Suitable, pharmaceutically acceptable salts of the compounds of formula (I) include, but are not limited to, salts with inorganic acids such as hydrochloride, sulfate, phosphate, diphosphate, hydrobromide, and nitrate, or salts with an organic acid such as malate, maleate, fumarate, tartrate, succinate, citrate, acetate, lactate, methanesulfonate, p-toluenesulfonate, palmitate, salicylate, and stearate. Salts may be prepared in a conventional manner using methods well known in the art. The invention also includes prodrugs of the aforementioned compounds. A prodrug is commonly described as an inactive or protected derivative of an active ingredient or a drug, which is converted to the active ingredient or drug in the body.
In addition, the invention extends to active derivatives of the aforementioned compounds.
Some of the compounds of this invention may be crystallised or recrystallised from solvents such as aqueous and organic solvents. In such cases solvates may be formed. This invention includes within its scope stoichiometric solvates including hydrates as well as compounds containing variable amounts of water that may be produced by processes such as lyophilisation. Certain of the compounds of formula (1) may exist in the form of optical isomers, e.g. diastereoisomers and mixtures of isomers in all ratios, e.g. racemic mixtures. The invention includes all such forms, in particular the pure isomeric forms. The different isomeric forms may be separated or resolved one from the other by conventional methods, or any given isomer may be obtained by conventional synthetic methods or by stereospecific or asymmetric syntheses. Where a compound contains an alkene moiety, the alkene can be presented as a cis or trans isomer or a mixture thereof. When an isomeric form of a compound of the invention is provided substantially free of other isomers, it will preferably contain less than 5% w/w, more preferably less than 2% w/w and especially less than 1% w/w of the other isomers. Since the compounds of formula (I) are intended for use in pharmaceutical compositions it will readily be understood that they are each preferably provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure and preferably at least 85%, especially at least 98% pure (% are on a weight for weight basis). Impure preparations of the compounds may be used for preparing the more pure forms used in the pharmaceutical compositions; these less pure preparations of the compounds should contain at least 1%, more suitably at least 5% and preferably at least 10% of a compound of the formula (I) or pharmaceutically acceptable derivative thereof.
The compounds of formula (I) can be prepared by art-recognized procedures from known or commercially available starting materials. If the starting materials are unavailable from a commercial source, their synthesis is described herein, or they can be prepared by procedures known in the art. The invention also provides a process for preparing a compound of formula (I) from a corresponding protected oxyamide intermediate compound, i.e. a compound as defined in formula (I), wherein the one or more hydroxamic acid groups (CONHOH) are replaced by CONHOP wherein P is a protecting group, for example, benzyloxyamide or tert-butyldimethylsilyloxyamide, by hydrogenation e.g. with palladium on a charcoal catalyst in dioxane or by cleavage with acid, e.g. hydrochloric acid or trifluoroacetic acid. The acid mediated removal of the tert-butyldimethylsilyl group can be performed as a separate step or can be performed as part of the work-up procedure.
The protected oxyamide intermediate compound may be prepared by conversion of the corresponding free acid of formula (II):
Figure imgf000006_0001
(II) wherein R1, R2, R3 and R5 are as defined for formula (I) and R4' is NR8CONR8RA where R8 is as defined for formula (I) and RΛ is Q_ι0 alkyl or C3_ι0 alkenyl wherein a -CH2- group other than that adjacent to the N may be replaced by -O- and wherein the alkyl or alkenyl is substituted with one or more carboxylic acid groups; by coupling with a protected hydroxylamine, for example, benzylhydroxylamine or te/-t- butyldimethylsilylhydroxylamine in THF under an argon atmosphere. An ester compound of formula (II) can be converted to a free acid compound of formula (H) by hydrolysis, using methods well known to those skilled in the art.
A compound of formula (II) may be prepared from a compound of formula (III):
Figure imgf000007_0001
(in) wherein Rx is N02, NHR8, or NCO and R1, R2, R3, R5 and R8 are as defined for formula (I), by the methods described below: A compound of formula (III) where Rx is NH2 may be prepared from a corresponding compound where Rx is N02 by methods well known to those skilled in the art, for example, hydrogenation with palladium on a charcoal catalyst or treatment with Zn and acetic acid. A compound of formula (III) wherein Rxis NH2 may be converted to a compound of formula (III) wherein Rx is NCO by reaction with, for example, triphosgene. A compound of formula (111) wherein Rx is NH2 may be converted to another compound of formula (HI) wherein Rx is NHR8 and R8 is Q.6 alkyl, by an alkylation or reductive amination reaction using methods well known to those skilled in the art.
A process for preparing a compound of formula (II), comprises: treating a compound of formula (III) wherein Rx is NHR8, with a compound of formula (IN):
RA— Ν=^0
(IN) wherein RA is Q_I0 alkyl or C3_ι0 alkenyl wherein a -CH2- group other than that adjacent to the Ν may be replaced by -O- and wherein the alkyl or alkenyl is substituted with one or more carboxylate ester groups, e.g. by stirring at room temperature or with heating in a suitable solvent, for example THF. Alternatively, a compound of formula (III) wherein Rx is ΝCO, is treated with a compound of formula (N):
RA— H2
(V) wherein RA is as defined for formula (IN), e.g. by stirring at room temperature or with heating in a suitable solvent. Compounds of formula (IN) and (N) may be available through the usual commercial sources. They and derivatives thereof may also be prepared by methods well known to those skilled in the art.
The compounds of formula (III) wherein Rx is Ν02, R1, R2, R3 and R5 are as defined for formula (I) and Z is O or S, may be prepared by treatment of a compound of formula (VI) wherein R1, R2 and R3 are as defined for formula (I) and RB is C02H or CHO:
Figure imgf000007_0002
(VI) with a compound of formula (Nil) wherein X and Y are as defined for formula (I) and Z is S or O:
(VII) by e.g. either (i) heating in a condensation/cyclisation reaction, using for example polyphosphoric acid, or (ii) by firstly coupling a compound of formula (VI) to a compound of formula (NH) via either an ester/ thioester or amide formation reaction using methods well known to those of skill in the art followed by direct heating or heating with an acidic media with a suitable solvent to effect cyclisation, for example, p- toluenesulfonic acid in toluene. Alternatively, this may be achieved via oxidative cyclisation of a Schiff base, derived from the condensation of the 2-aminophenol or 2-aminothiophenol and aldehydes, using various oxidants such as PhI(OAc)2, Pb(OAc) or DDQ.
Compounds of formulae (NI) and (Nil) may be available through the usual commercial sources. They and derivatives thereof may also be prepared by methods well known to those skilled in the art. The compounds of formula (III) wherein Rx is Ν02, R1, R2, R3 and R5 are as defined for formula
(I) and Z is NR8, may be prepared by treatment of a compound of formula (NI) with a compound of formula (NIIl):
Figure imgf000008_0001
(VIII) wherein X, Y and R8 are as defined for formula (I) in e.g. the following reactions:
(i) if RB in compound (VI) is C02H, heating in a condensation/cyclisation reaction using for example polyphosphoric acid; or
(ii) if RB in compound (NI) is CHO, heating in acetonitrile followed by oxidation using for example 02 FeCl3 (cat.) in acetonitrile. Compounds of formula (NIH) may be prepared by reduction of compounds of formula (IX) wherein X, Y and R8 are as defined for formula (I) by methods well known to those skilled in the art, for example, hydrogenation with palladium on a charcoal catalyst or treatment with Zn and acetic acid:
Figure imgf000008_0002
(K)
Compounds of formula (IX) may be prepared by nitration of compounds of formula (X) wherein X, Y and R8 are as defined for formula (I), by methods well known to those skilled in the art, for example using fuming nitric acid and sulfuric acid or fuming nitric acid and tin(JN) chloride:
Figure imgf000008_0003
(X) Compounds of formula (X) may be available through the usual commercial sources. They and derivatives thereof may also be prepared by methods well known to those skilled in the art.
The compounds of formula (III), wherein X or Y is halogen, can be modified to give a corresponding set of compounds of formula (IH) wherein X or Y is phenyl or a 5- to 10-membered heteroaryl group either of which is optionally substituted by one or more substituents as defined in formula (I). The modification may be achieved by a coupling reaction with compounds of formula (XI) using an appropriate catalyst for example tetrakis (triphenylphosphine) palladium: Rc^B(OH)2
(XI) where Rc is phenyl or a 5- to 10-membered heteroaryl group either of which is optionally substituted by one or more substituents as defined for formula (I).
Additionally, compounds of formula (111) where Rx is N02, R3 is halogen at a position ortho or para to the Rx group and R1, R2 and R5 are as defined for formula (I) may be converted to a corresponding subset of compounds of formula (HI) where R3 is OR6 or NR7R8, by reaction with an alcohol or amine via a nucleophilic aromatic substitution. Compounds of formula (III) where R3 is NR8COR10 or NR8S02R10 may be prepared from corresponding compounds of formula (III) where R3 is NHR8 by reaction with the appropriate carboxylic acid/chloride or sulfonyl chloride, i.e. R10CO2H/(R10CO2Cl) or R10SO2Cl wherein R10 is as defined for formula (1).
During the synthesis of the compounds of formula (1), labile functional groups in the intermediate compounds, e.g. hydroxy, carboxy and amino groups, may be protected. The protecting groups may be removed at any stage in the synthesis of the compounds of formula (1) or may be present on the final compound of formula (I). A comprehensive discussion of the ways in which various labile functional groups may be protected and methods for cleaving the resulting protected derivatives is given in for example Protective Groups in Organic Chemistry, T.W. Greene and P.G.M. Wuts (Wiley-Interscience, New York, 2nd edition, 1991).
Further details for the preparation of compounds of formula (I) are found in the examples. The compounds of formula (I) may be prepared singly or as compound libraries comprising at least 2, for example 5 to 1,000 compounds, and more preferably 10 to 100 compounds of formula (I). Libraries of compounds of formula (I) may be prepared by multiple parallel synthesis using either solution phase or solid phase chemistry, by procedures known to those skilled in the art.
Thus according to a further aspect of the invention there is provided a compound library comprising at least 2 compounds of formula (I) or pharmaceutically acceptable salts and prodrugs thereof.
The pharmaceutically acceptable salts and prodrugs of the compounds of formula (I) may be prepared by methods well known to those skilled in the art. Any novel intermediate compounds as described herein also fall within the scope of the present invention, fn particular the invention provides a compound as defined by formula (I) wherein the one or more hydroxamic acid groups (CONHOH) are replaced by CONHOP wherein P is a protecting group e.g. benzyloxyamide, tert-butyldimethylsilyloxyamide or tert-butyldiphenylsilyloxyamide. Preferred intermediate compounds include those described in the examples. The pharmaceutically effective compounds of formula (I) may be administered in conventional dosage forms prepared by combining a compound of formula (I) ("active ingredient") with standard pharmaceutical carriers or excipients according to conventional procedures well known in the art. These procedures may involve mixing, granulating and compressing or dissolving the ingredients as appropriate to the desired preparation. According to a further aspect of the invention there is provided a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt or prodrug thereof, together with one or more pharmaceutically acceptable carriers or excipients.
The active ingredient or pharmaceutical composition can be administered simultaneously, separately or sequentially with another appropriate treatment for cancer, angiogenesis, inflammatory and autoimmune conditions, cardiovascular diseases and proliferative disorders of the skin. The active ingredient or pharmaceutical composition may be administered to a subject by any of the routes conventionally used for drug administration, for example they may be adapted for oral (including buccal, sublingual), topical (including txansdermal), nasal (including inhalation), rectal, vaginal or parenteral (including subcutaneous, intramuscular, intravenous or intradermal) administration to mammals including humans. The most suitable route for administration in any given case will depend on the particular compound or pharmaceutical composition, the subject, and the nature and severity of the disease and the physical condition of the subject. Such compositions may be prepared by any method known in the art of pharmacy, for example by bringing into association the active ingredient with the carrier(s) or excipient(s). Pharmaceutical compositions adapted for oral administration may be presented as discrete units such as capsules or tablets; powders or granules; solutions or suspensions in aqueous or non-aqueous liquids; edible foams or whips; or oil-in-water liquid emulsions or water-in-oil liquid emulsions.
Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants, for example potato starch; or acceptable wetting agents such as sodium lauryl sulfate. The tablets may be coated according to methods well known in normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives, such as suspending agents, for example sorbitol, methyl cellulose, glucose syrup, gelatin, hydroxyethyl cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl^-hydroxybenzoate or sorbic acid, and, if desired, conventional flavouring or colouring agents.
Pharmaceutical compositions adapted for topical administration may be formulated as ointments, creams, suspensions, lotions, powders, solutions, pastes, gels, impregnated dressings, sprays, aerosols or oils and may contain appropriate conventional additives such as preservatives, solvents to assist drug penetration and emollients in ointments and creams. Such applications include those to the eye or other external tissues, for example the mouth and skin and the compositions are preferably applied as a topical ointment or cream. When formulated in an ointment, the active ingredient may be employed with either a paraffinic or a water- miscible ointment base. Alternatively, the active ingredient may be formulated in a cream with an oil-in- water cream base or a water-in-oil base. The composition may also contain compatible conventional carriers, such as cream or ointment bases and ethanol or oleyl alcohol for lotions.
Pharmaceutical compositions adapted for topical administration to the eye include eye drops wherein the active ingredient is dissolved or suspended in a suitable carrier, especially an aqueous solvent.
Pharmaceutical compositions adapted for topical administration in the mouth include lozenges, pastilles and mouth washes.
Pharmaceutical compositions adapted for transdermal administration may be presented as discrete patches intended to remain in intimate contact with the epidermis of the recipient for a prolonged period of time. For example, the active ingredient may be delivered from the patch by iontophoresis as generally described in Pharmaceutical Research, 3(6), 318 (1986). Pharmaceutical compositions adapted for nasal administration wherein the carrier is a solid include a coarse powder having a particle size for example in the range 20 to 500 microns which is administered by rapid inhalation through the nasal passage from a container of the powder held close up to the nose. Suitable compositions wherein the carrier is a liquid, for administration as a nasal spray or as nasal drops, include aqueous or oil solutions of the active ingredient.
Pharmaceutical compositions adapted for administration by inhalation include fine particle dusts or mists which may be generated by means of various types of metered dose pressurised aerosols, nebulizers or insufflators.
Pharmaceutical compositions adapted for rectal administration may be presented as suppositories or enemas. Suppositories will contain conventional suppository bases, e.g. cocoa-butter or other glyceride. Pharmaceutical compositions adapted for vaginal administration may be presented as pessaries, tampons, creams, gels, pastes, foams or spray compositions.
Pharmaceutical compositions adapted for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The compositions may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example water for injections, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets.
For parenteral administration, fluid unit dosage forms are prepared utilizing the active ingredient and a sterile vehicle, water being preferred. The active ingredient, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions the active ingredient can be dissolved in water for injection and filter sterilised before filling into a suitable vial or ampoule and sealing.
Advantageously, agents such as a local anaesthetic, preservative and buffering agents can be dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. The dry lyophilized powder is then sealed in the vial and an accompanying vial of water for injection may be supplied to reconstitute the liquid prior to use. Parenteral suspensions are prepared in substantially the same manner except that the active ingredient is suspended in the vehicle instead of being dissolved and sterilization cannot be accomplished by filtration. The active ingredient can be sterilised by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the active ingredient. The pharmaceutical compositions according to the invention are preferably adapted for oral administration.
It should be understood that in addition to the ingredients particularly mentioned above, the compositions may also include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents. They may also contain therapeutically active agents in addition to the compounds of the present invention. Such carriers may be present as from about 1% up to about 98% of the formulation. More usually they will form up to about 80%) of the formulation.
The compositions may contain from 0.1% by weight, preferably from 10-60% by weight, of the active material, depending on the method of administration. Pharmaceutical compositions may be presented in unit dose forms containing a predetermined amount of active ingredient per dose. Such a unit may contain for example 0. lmg/kg to 750mg/kg, more preferably 0. lmg/kg to lOmg/kg depending on the condition being treated, the route of aαteinistration and the age, weight and condition of the patient. Preferred unit dosage compositions are those containing a daily dose or sub-dose, as herein above recited, or an appropriate fraction thereof, of an active ingredient.
It will be recognized by one of skill in the art that the optimal quantity and spacing of individual dosages of compounds in the first and second aspects of the invention will be determined by the nature and extent of the condition being treated, the form, route and site of administration, and the particular subject being treated, and that such optimums can be determined by conventional techniques. It will also be appreciated by one of skill in the art that the optimal course of treatment, i.e., the number of doses of • the aforementioned compounds given per day for a defined number of days, can be ascertained by those skilled in the art using conventional course of treatment determination tests.
No toxicological effects are indicated when the aforementioned compounds of formula (I) are administered in the above mentioned dosage range. The compounds of the present invention are useful in that they are capable of inhibiting histone deacetylase activity. Thus, the compounds can be used in the treatment of cancer.
The compounds of the present invention can also be used in combination with one or more additional treatments or therapeutic compounds for cancer. Examples of such treatments include, surgery and radiation therapy. Examples of therapeutic compounds include but are not limited to cisplatin, cyclophosphamide, methotrexate, 5-fluorouracil, paclitaxel, docetaxel, vincristine, vinblastine, vinorelbine, doxorubicin, tamoxifen, toremifene, megestrol acetate, anastrozole, goserelin, anti-HER2 monoclonal antibody, capecitabine and raloxifene hydrochloride.
The compounds of the present invention can also be used in the treatment of angiogenesis and angiogenesis dependent diseases, which include angiogenesis associated with the growth of solid tumours and retinopathy.
The compounds of the present invention can also be used in combination with one or more additional treatments or therapeutic compounds for angiogenesis. Examples of such other therapeutic compounds include, but are not limited to, recombinant platelet-derived growth factor-BB (Regranex™).
The compounds of the present invention can also be used in the treatment of inflammatory conditions, including, but not limited to, rheumatoid arthritis, inflammatory bowel disease, and wound healing.
The compounds of the present invention can also be used in the treatment of proliferative skin disorders, such as, but not limited to psoriasis.
By the term "treating" is meant either prophylactic or therapeutic therapy. The term cancer or 'carcinoma' is a malignant new growth that arises from epithelium, found in skin or, more commonly, the lining of body organs. Carcinomas tend to infiltrate into adjacent tissues and spread (metastasise) to distant organs, for example to bone, liver lung or the brain. Herein, cancer includes both metastatic tumour cells and tissue and examples include, but are not limited to, melanoma, mesothelioma, lymphoma, leukaemia, fibrosarcoma, rhabdomyosarcoma, mastocytoma and the following tissue -carcinomas: colorectal, colon, prostate, lung, breast, pancreatic, intestinal, renal, gastric, bladder, ovarian, uterine, cervical, hepatic and stomach.
In additional aspects, therefore, the present invention provides:
(i) the use of a compound of formula (I) in the manufacture of a medicament for the inhibition of histone deacetylase enzyme activity. (ii) the use of a compound of formula (I) in the manufacture of a medicament for the treatment of cancer.
(iii) the use of a compound of formula (I) in the manufacture of a medicament for the treatment of angiogenesis and angiogenesis dependent diseases which include angiogenesis associated with the growth of solid tumours and retinopathy.
(iv) the use of a compound of formula (I) in the manufacture of a medicament for the treatment of inflammatory conditions such as but not limited to rheumatoid arthritis, inflammatory bowel disease, and wound healing.
(v) a method for the treatment of cancer, which comprises the step of administering to a patient an effective amount of a compound of formula (1) .
(vi) a method for the treatment of angiogenesis and angiogenesis dependent diseases, which include angiogenesis associated with the growth of solid tumours and retinopathy, which comprises the step of administering to a patient an effective amount of a compound of formula (I).
(vii) a method for the treatment of inflammatory diseases, such as but not limited to rheumatoid arthritis, inflammatory bowel disease, and wound healing which comprises the step of administering to a patient an effective amount of a compound of formula (I).
All publications, including, but not limited to, patents and patent applications cited in this specification, are herein incorporated by reference as if each individual publication were specifically and individually indicated to be incorporated by reference herein as though fully set forth. The invention will now be described by reference to the following examples, which are merely illustrative and are not to be construed as a limitation of the scope of the present invention.
Example 1:
6- [3- [2-Methoxy-5-(5-phenylbenzoxazole-2-yl)pheιryl] ureido] hexanoic acid hydroxyamide (a) 2-(3-Amino-4-methoxyphenyl)-5-phenylbenzoxazole
Figure imgf000013_0001
3-Amino-4-methoxybenzoic acid (3.0mmol) and 2-amino-4-phenylphenol (3.0mmol) were mixed in polyphosphoric acid (5ml) and the mixture was heated to 200°C for 4 h. The reaction mixture was slowly poured into ice water (100ml) and the resulting mixture basified with solid sodium hydroxide. At pH5-6 the precipitate was filtered, washed with water and dried under vacuum. MS (APCI-) m/z 317. (b) 6-[3-[2-Methoxy-5-(5-phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000013_0002
Ethyl 6-isocyanatohexanoate (36μL, 0.2mmol) was added to a solution of 2-(3-amino-4-methoxy- phenyl)-5-phenylbenzoxazole (0.2mmol) in THF (2ml). After stirring overnight at 40°C the reaction was concentrated under reduced pressure to give the product. MS (APCI-) m/z 500.1 (c) 6-[3-[2-Methoxy-5-(5-phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid
Figure imgf000014_0001
6-[3-[2-Methoxy-5-(5-phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic ethyl ester (0.2mmol) in THF/MeOH (1:1, 1.5ml) was added to a solution of LiOH (24mg 1.0 mmol) in water (0.5ml). After stirring for 6h the reaction was acidified with 2M HCI and the resulting precipitate filtered and dried under vacuum. I NMR (DMSO): δ 1.21-1.50 (6H, m), 2.23 (2H, t), 3.11 (2H, q), 3.96 (3H, s), 6.98 (IH, t), 7.19 (IH, d), 7.39 (IH, m), 7.49 (2H, t), 7.75 (5H, m), 8.00 (IH, d), 8.13 (IH, s), 9.08 (IH, d). MS (APCI-) m/z 472.0. (d) 6-[3 -[2-Methoxy-5 -(5 -phenylbenzoxazole-2-yl)phenyι]ureido]hexanoic acid benzyloxyamide
Figure imgf000014_0002
To a stirred solution of 6-[3-[2-methoxy-5-(5-phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid (200mg, 0.42mmol) in THF (10ml) under an argon atmosphere was added 2-chloro-4,6-dimethoxy- 1,3,5-triazine (89mg, 0.51mmol) followed by 4-methylmorpholine (128mg, 1.27mmol). After stirring for lh, benzylhydroxylamine hydrochloride (67.4mg, 0'.42mmol) was added. After stirring overnight the reaction was quenched with water (10ml) and extracted with ethyl acetate (2x50ml). The organic fractions were combined and washed with saturated sodium hydrogen carbonate solution (50ml), 2M hydrochloric acid solution (50ml), saturated sodium chloride solution (50ml), dried (Na2S04) and concentrated. This crude product was then subjected to flash silica gel column chromatography using ethyl acetate as eluant to give the subtitle compound, 47mg (19%). *H NMR (DMSO) δ 1.25 (2H, m), 1.48 (4H, ), 1.97 (2H, t), 3.11 (2H, m), 3.94 (3H, s), 4.76 (2H, s), 6.98 (IH, m), 7.19 (IH, d), 7.36 (6H, m), 7.50 (2H, t), 7.65-7.87 (5H, m), 8.01 (IH, d), 8.16 (IH, s), 9.09 (IH, s), 10.97 (IH, s). MS (APCI-) m/z 577. (e) 6-[3-[2-Methoxy-5-(5-phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000014_0003
To a stirred solution of 6-[3-[2-methoxy-5-(5-phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid benzyloxyamide (41mg, 0.07mmol) in dioxane (10ml) under an argon atmosphere was added 10% Pd on charcoal (80mg). The flask was then evacuated and flushed with hydrogen. After stirring for 2 days the reaction mixture was filtered through a pad of celite, washed with further dioxane (50ml) and concentrated to give the title compound 25mg (75%). Η NMR (DMSO) δ 1.30 (2H, m), 1.50 (4H, m), 1.98 (2H, m), 3.10 (2H, m), 3.95 (3H, s), 7.00 (IH, m), 7.20 (IH, d), 7.38 (IH, m), 7.50 (2H, t), 7.65-7.88 (5H, m), 8.03 (IH, d), 8.15 (IH, s), 8.59 (IH, s), 9.05 (IH, s), 10.36 (IH, s). MS (APCI-) m/z 487.
Example 2: 6-[3-[2-Methoxy-5-(benzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
(a) 2-(4-Methoxy-3 -nitrophenyl)benzoxazole
Figure imgf000015_0001
To a stirred solution of 2-aminophenol (2.77g, 25.4mmol) in tetrahydrofuran (100ml) was added dropwise a solution of 4-methoxy-3-nitrobenzoyl chloride (5.47g, 25.4mmol). After stirring overnight the solvent was removed under reduced pressure. The solid was suspended in toluene (150ml) and j3-toluenesulfonic acid (10.13g, 53.3mmol) was added. This was heated to reflux for 4h under Dean Stark conditions. The solution was allowed to cool and partitioned between ethyl acetate (100ml) and saturated sodium hydrogen carbonate solution (200ml). The aqueous phase was extracted with ethyl acetate (2x50ml) and the combined organic extracts were washed with 2M hydrochloric acid (2x50ml), water (50ml), saturated sodium chloride solution (50ml), dried (Na2S04) and concentrated to give the subtitle compound (6.83g, 99%). Η NMR (DMSO): δ 4.05 (3H, s), 7.43-7.45 (2H, m), 7.62 (IH, d), 7.79-7.83 (2H, m), 8.45 (IH, dd), 8.63 (IH, d).
(b) 2-(3 -Amino-4-methoxyphenyl)benzoxazole
Figure imgf000015_0002
To a suspension of 2-(4-methoxy-3-nitrophenyl)benzoxazole (3 , 11.1 mmol) in ethyl acetate (200ml) under an argon atmosphere was added 10% Pd on carbon (0.75g). The flask was flushed with hydrogen and the reaction was stirred overnight. The mixture was filtered through a pad of Celite and the solvent was removed under reduced pressure. The crude compound was subjected to flash silica gel column chromatography using light petroleum-ethyl acetate (2:1/1:1) as eluant to give the subtitle compound (2.1 lg, 79%). 'H MR (DMSO): δ 3.87 (3H, s), 5.13 (2H, s), 6.99 (IH, d), 7.34-7.39 (2H, m), 7.43 (IH, dd), 7.51 (IH, d), 7.70-7.75 (2H, m). (c) 6-[3-[5-(Benzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid ethyl ester
Figure imgf000015_0003
To a stirred solution of 2-(3-amino-4-methoxyphenyl)benzoxazole (l.OOg, 4.16mmol) in tetrahydrofuran (30ml) was added ethyl 6-isocyanatohexanoate (1.12ml, 6.24mmol) dropwise. The reaction was refluxed at 80°C for 48h. The reaction mixture was allowed to cool and the solvent was removed under reduced pressure. The residue was triturated in ethyl acetate and the solid collected by filtration and dried under vacuum to give the subtitle compound (1.36g, 77%). H NMR (DMSO): δ 1.17 (3H, t), 1.28-1.36 (2H, m), 1.40-1.47 (2H, m), 1.51-1.61 (2H, m), 2.30 (2H, t), 3.10 (2H, q), 3.95 (3H, s), 4.04 (2H, q), 6.98 (IH, t), 7.19 (IH, d), 7.35-7.41 (2H, m), 7.73-7.80 (3H, m), 8.13 (IH, s), 9.06 (IH, d). (d) 6-[3-[5-(Benzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid
Figure imgf000016_0001
A solution of lithium hydroxide (0.319g, 13.33mmol) in water (7ml) was added to a solution of 6- [3-[5-(benzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid ethyl ester (1.13g, 2.67mmol) in tetrahydrofuran (21ml) and the reaction was stirred vigorously at 50°C overnight. The resulting suspension was acidified with 2M hydrochloric acid and the precipitate was collected by filtration to give the subtitle compound (0.95g, 90%). »H NMR (DMSO): δ 1.28-1.36 (2H, m), 1.40-1.58 (4H, m), 2.23 (2H, t), 3.11 (2H, q), 3.95 (3H, s), 6.98 (IH, t), 7.19 (IH, d), 7.35-7.40 (2H, m), 7.74-7.78 (3H, m), 8.13 (IH, s), 9.06 (IH, d), 12.04 (IH, bs). (e) 6-[3-[5-(Benzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000016_0002
To a stirred suspension of 6-[3-[5-(benzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid (500mg, 1.26mmol) in tetrahydofuran (50ml) under an argon atmosphere was added 2-chloro-4,6- dimefhoxy-l,3,5-triazine (376mg, 2.14mmol) followed by N-methylmorpholine (0.41ml, 3.77mmol). After stirring for 1 h tert-butyldimethylsilyl hydroxylamine (278mg, 1.89mmol) was added. After stirring over 48 hours the reaction mixture was quenched with water (50ml) and extracted with ethyl acetate (3x50ml). The combined organic extracts were washed with saturated sodium hydrogen carbonate solution (2x50ml) followed by 2M hydrochloric acid (2x50ml). The product, which precipitated during the 2M hydrochloric acid treatment, was collected by filtration, washed with water and freeze dried to give the title compound, (0.159g, 30%). !H NMR (DMSO): δ 1.22 (2H, m), 1.39-1.57 (4H, m), 1.96 (2H, t), 3.10 (2H, q), 3.95 (3H, s), 6.98 (IH, t), 7.19 (IH, d), 7.35-7.42 (2H, m), 7.73-7.80 (3H, m), 8.13 (IH, s), 8.69 (IH, d), 9.06 (IH, d), 10.36 (IH, bs). MS (APCI+) m/z 413.2, (APCI-) m/z 411.0.
Example 3: 6-[3-[3-(Benzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
(a) 2-(3-Nitrophenyl)benzoxazole
Figure imgf000017_0001
Prepared by the method of Example 2a) from 2-aminophenol (1.63g, 15.0mmol) and 3- nitrobenzoyl chloride (2.78g, 15.0mmol) the subtitle compound was obtained, (2.82g, 78%). !H NMR (DMSO): δ 7.44-7.53 (2H, m), 7.86-7.96 (3H, m), 8.47 (IH, ), 8.61 (IH, d), 8.87 (IH, s). (b) 2-(3-Aminophenyl)benzoxazole
Figure imgf000017_0002
Prepared by the method of Example 2b) from 2-(3-nitrophenyl)benzoxazole (2.82g, 11.7mmol) the subtitle compound was obtained, (2.40g, 97%). 'H MR (DMSO): δ 5.50 (2H, s), 6.78-6.81 (IH, m), 7.23 (IH, t), 7.33-7.44 (4H, m), 7.77 (2H, m).
(c) 6-[3-[3-(Benzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000017_0003
Prepared by the method of Example 2c) from 2-(3-aminophenyl)benzoxazole (l.OOg, 4.76mmol) and ethyl 6-isocyanatohexanoate (1.28ml, 7.13mmol) the subtitle compound was obtained, (1.41g, 75%).
TJ NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.41-1.48 (2H, m), 1.50-1.60 (2H, m), 2.30 (2H, t),
3.10 (2H, q), 4.05 (2H, m), 6.24 (IH, t), 7.41-7.47 (4H, m), 7.72-7.82 (3H, m), 8.49 (IH, d), 8.77 (IH, s).
(d) 6-[3-[3-(Benzoxazole-2-yl)phenyl]ureido]hexanoic acid
Figure imgf000017_0004
Prepared by the method of Example 2d) from 6-[3-[3-(benzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester (1.41g, 3.57mmol) and lithium hydroxide (0.43 g, 17.84mmol) the subtitle compound was obtained, (1.08g, 82%). If NMR (DMSO): δ 1.27-1.35 (2H, m), 1.41-1.58 (4H, m), 2.22 (2H, t), 3.10 (2H, q), 6.24 (IH, t), 7.41-7.47 (4H, m), 7.73 (IH, d), 7.79-7.82 (2H, m), 8.49 (IH, s), 8.77 (IH, s), 12.02 (IH, bs).
(e) 6-[3-[3-(Benzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000018_0001
Prepared by the method of Example 2e) from 6-[3-[3-(benzoxazole-2-yl)phenyl]ureido]hexanoic acid (500mg, 1.36mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (406mg, 2.31mmol), N- methylmorpholine (0.45ml, 4.08mmol) and tert-butyldimethylsilyl hydroxylamine (301mg, 2.04mmol). The combined organic extracts were washed with saturated sodium hydrogen carbonate solution
(2x50ml) and then stirred vigorously with 2M hydrochloric acid for 4.5h. The resultant precipitate was collected by filtration, washed with water and freeze dried to give the title compound (191mg, 37%). XH NMR (DMSO): δ 1.22-1.32 (2H, m), 1.40-1.57 (4H, m), 1.96 (2H, t), 3.10 (2H, q), 6.24 (IH, t), 7.38-7.50 (4H, m), 7.72-7.74 (IH, m), 7.78-7.84 (2H, m), 8.49 (IH, s), 8.69 (IH, s), 8.77 (IH, s), 10.36 (IH, bs). MS (APCI+) m/z 383.2, (APCI-) m/z 381.0.
Example 4:
6- [3- [3-(5-Fluorobenzoxazole-2-yl)phenyl] ur eido] hexanoic acid hydroxyamide
(a) 2-(3 -Nitroρhenyl)-5 -fluorobenzoxazole
Figure imgf000018_0002
Prepared by the method of Example 2a) from 2-amino-4-fluorophenol (309mg, 2.43mmol) and 3- nitrobenzoyl chloride (500mg, 2.69mmol) the subtitle compound was obtained, (490mg, 78%). !H NMR (DMSO): δ 7.38 (IH, dt), 7.79 (IH, dd), 7.89-7.96 (2H, m), 8.48 (IH, dd), 8.60 (IH, d), 8.85 (IH, t). (b) 2-(3-Aminophenyl)-5-fluorobenzoxazole
Figure imgf000018_0003
Prepared by the method of Example 2b) from 2-(3-nitrophenyl)-5 -fluorobenzoxazole (490mg, 1.90mmol) the subtitle compound was obtained, (419mg, 97%). *H NMR (DMSO): δ 5.52 (2H, bs), 6.80 (IH, m), 7.21-7.34 (3H, m), 7.42 (IH, s), 7.66 (IH, dd), 7.80 (IH, dd).
(c) 6-[3-[3-(5-Fluorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000019_0001
Prepared by the method of Example 2c) from 2-(3-aminophenyl)-5-fluorobenzoxazole (419mg, 1.84mmol) and ethyl 6-isocyanatohexanoate (0.49ml, 2.73mmol) the subtitle compound was obtained, (484mg, 64%). *H NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.41-1.60 (4H, m), 2.30 (2H, t), 3.10 (2H, q), 4.05 (2H, q), 6.24 (IH, t), 7.30 (IH, dt), 7.42-7.50 (2H, m), 7.67-7.73 (2H, m), 7.85 (IH, dd), 8.49 (IH, s), 8.78 (IH, s). (d) 6-[3-[3-(5-Fluorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid
Figure imgf000019_0002
Prepared by the method of Example 2d) from 6-[3-[3-(5-fluorobenzoxazole-2- yl)phenyl]ureido]hexanoic acid ethyl ester (484mg, 1.17mmol) and lithium hydroxide (140mg, 5.85mmol) the subtitle compound was obtained, (445mg, 99%). Η NMR (DMSO): δ 1.26-1.35 (2H, m), 1.41-1.58 (4H, m), 2.22 (2H, t), 3.10 (2H, q), 6.32 (IH, t), 7.30 (IH, dt), 7.42-7.51 (2H, m), 7.68-7.73 (2H, m), 7.85 (IH, dd), 8.48 (IH, s), 8.89 (IH, s), 12.01 (IH, bs). e) 6-[3-[3-(5-Fluorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000019_0003
Prepared by the method of Example 2e) from 6-[3-[3-(5-fluorobenzoxazole-2-yl)phenyl]ureido]- hexanoic acid (291mg, 0.76mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (225mg, 1.28mmol), N-methyl morpholine (0.25ml, 2.27mmol) and tert-butyldimethylsilyl hydroxylamine (167mg, 1.13mmol). The combined organic extracts were washed with saturated sodium hydrogen carbonate solution (2x50ml) and then stirred vigorously with 2M hydrochloric acid for 2.5h. The resultant precipitate was collected by filtration, washed with water and freeze dried to give the title compound (55mg, 18%). H NMR (DMSO): δ 1.22-1.32 (2H, m), 1.40-1.57 (4H, m), 1.96 (2H, t), 3.09 (2H, q), 6.24 (IH, t), 7.31 (IH, dt), 7.42-7.50 (2H, m), 7.68-7.73 (2H, m), 7.85 (IH, dd), 8.49 (IH, s), 8.69 (IH, d), 8.79 (IH, s), 10.36 (IH, s). MS (APCI-) m/z 399.0.
Example 5:
6-[3-[3-(5-Methoxybenzoxazole-2-yI)phenyl]ureido]hexanoic acid hydroxyamide
(a) 2-(3 -Nitrophenyl)-5 -methoxybenzoxazole
Figure imgf000020_0001
Prepared by the method of Example 2a) from 2-amino-4-methoxyphenol (338mg, 2.43mmol) and
3-nitrobenzoyl chloride (500mg, 2.69mmol) the subtitle compound was obtained, (251mg, 69%). H NMR (DMSO): δ 3.84 (3H, s), 7.07 (IH, dd), 7.41 (IH, d), 7.75 (IH, d), 7.91 (IH, t), 8.45 (IH, m), 8.56 (IH, m), 8.82 (IH, t).
(b) 2-(3 -Aminophenyl)-5 -methoxybenzoxazole
Figure imgf000020_0002
Prepared by the method of Example 2b) from 2-(3-nitrophenyl)-5 -methoxybenzoxazole (452mg, 1.67mmol) the subtitle compound was obtained, (369mg, 92%). Η NMR (DMSO): δ 3.82 (3H, s), 5.49 (2H, bs), 6.79 (IH, m), 6.98 (IH, dd), 7.22 (IH, t), 7.29-7.33 (2H, m), 7.40 (IH, s), 7.65 (IH, d). (c) 6~[3-[3-(5-Methoxybenzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000020_0003
Prepared by the method of Example 2c) from 2-(3-aminophenyl)-5 -methoxybenzoxazole (369mg, 1.54mmol) and ethyl 6-isocyanatohexanoate (0.41ml, 2.28mmol) the subtitle compound was obtained, (359mg, 55%). Η NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.41-1.60 (4H, m), 2.30 (2H, t), 3.10 (2H, q), 3.83 (3H, s), 4.05 (2H, q), 6.23 (IH, t), 6.01 (IH, dd), 7.36 (IH, d), 7.40-4.49 (2H, m), 7.68-7.70 (2H, m), 8.44 (IH, s), 8.76 (IH, s). (d) 6-[3-[3-(5-Methoxybenzoxazole-2-yl)phenyl]ureido]hexanoic acid
Figure imgf000021_0001
Prepared by the method of Example 2d) from 6-[3-[3-(5-methoxybenzoxazole-2- yl)phenyl]ureido]hexanoic acid ethyl ester (359mg, 0.84mmol) and lithium hydroxide (lOlmg, 4.22mmol) the subtitle compound was obtained, (307mg, 92%). *H NMR (DMSO): δ 1.26-1.35 (2H, m), 1.41-1.58 (4H, m), 2.22 (2H, t), 3.10 (2H, q), 3.83 (3H, s), 6.25 (IH, t), 7.01 (IH, dd), 7.36 (IH, d), 7.41- 7.50 (2H, m), 7.70 (2H, d), 8.44 (IH, s), 8.79 (IH, s). (e) 6-[3-[3-(5-Methoxybenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000021_0002
Prepared by the method of Example 2e) from 6-[3 -[3 -(5-methoxybenzoxazole-2- yl)phenyl]ureido]hexanoic acid (300. lmg, 0.76mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (225.3mg, 1.28mmol), N-methylmorpholine (0.25ml, 2.27mmol) and te7-t-butyldimethylsilyl hydroxylamine. The combined organic extracts were washed with saturated sodium hydrogen carbonate solution (2x50ml) and then stirred vigorously with 2M hydrochloric acid for 20min. The resultant precipitate was collected by filtration, washed with water and freeze dried to give the title compound (174mg, 55%). *H NMR
(DMSO): δ 1.22-1.32 (2H, m), 1.40-1.57 (4H, m), 1.96 (2H, t), 3.09 (2H, q), 3.83 (3H, s), 6.23 (IH, t), 7.01 (IH, dd), 7.36 (IH, d), 7.41-7.49 (2H, m), 7.70 (2H, d), 8.44 (IH, s), 8.69 (IH, d), 8.76 (IH, s), 10.36 (IH, s). MS (APCI-) m/z 411.0.
Example 6:
6-[3-[3-(5-Trifluoromethylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
(a) 2-(3 -Nitrophenyl)-5 -trifluoromethylbenzoxazole
O r=
?3C Jθ3 N02 Prepared by the method of Example 2a) from 2-amino-4-(trifluoromethyl)phenol (429.9mg, 2.43mmol) and 3-nitrobenzoyl chloride (500mg, 2.69mmol) the subtitle compound was obtained, (602mg, 80%). *H NMR (DMSO): δ 7.87 (IH, dd), 7.95 (IH, t), 8.11 (IH, d), 8.32 (IH, s), 8.49-8.53 (IH, m), 8.63 (IH, d), 8.88 (IH, t). (b) 2-(3-Aminophenyl)-5-trifluoromethylbenzoxazole F,C .XX, ^ /
NH,
Prepared by the method of Example 2b) from 2-(3-nitrophenyl)-5-trifluoromethylbenzoxazole (602mg, 1.95mmol) the subtitle compound was obtained, (521mg, 96%). Η NMR (DMSO): δ 5.56 (2H, bs), 6.83 (IH, m), 7.26 (IH, t), 7.37 (IH, d), 7.46 (IH, s), 7.78 (IH, d), 8.01 (IH, d), 8.20 (IH, d). c) 6-[3-[3-(5-Trifluoromethylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000022_0001
Prepared by the method of Example 2c) from 2-(3-aminophenyl)-5-trifluoromethylbenzoxazole (521mg, 1.87mmol) and ethyl 6-isocyanatohexanoate (0.50ml, 2.79mmol) the subtitle compound was obtained, (704mg, 81%). Η NMR (DMSO): δ 1.17 (3H, t), 1.27-1.35 (2H, m), 1.41-1.60 (4H, m), 2.30 (2H, t), 3.10 (2H, q), 4.05 (2H, q), 6.25 (IH, t), 7.44-7.52 (2H, m), 7.74-7.82 (2H, m), 8.03-8.05 (IH, d), 8.24 (IH, s), 8.54 (IH, s), 8.81 (IH, s). (d) 6-[3 -[3 -(5 -Trifluoromethylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid
Figure imgf000022_0002
Prepared by the method of Example 2d) from 6-[3-[3-(5-trifluoromethylbenzoxazole-2- yl)phenyl]ureido]hexanoic acid ethyl ester (704mg, 1.52mmol) and lithium hydroxide (182mg, 7.59mmol) the subtitle compound was obtained, (621mg, 94%). !H NMR (DMSO): δ 1.26-1.35 (2H, m), 1.41-1.58 (4H, m), 2.22 (2H, t), 3.11 (2H, q), 6.26 (IH, t), 7.45-7.53 (2H, m), 7.75-7.82 (2H, m), 8.05 (IH, d), 8.24 (IH, s), 8.54 (IH, s), 8.81 (IH, s), 12.01 (IH, bs).
(e) 6-[3-[3-(5-Trifluoromethylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000022_0003
Prepared by the method of Example 2e) from 6-[3-[3-(5-frifluoromethylbenzoxazole-2- yl)phenyl]ureido]hexanoic acid (329mg, 0.76mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (225.3mg, 1.28mmol), N-methylmorpholine (0.25ml, 2.27mmol) and tert-butyldimethylsilyl hydroxylamine. The combined organic extracts were washed with saturated sodium hydrogen carbonate solution (2x50ml) and then stirred vigorously with 2M hydrochloric acid for 2h. The resultant precipitate was collected by filtration, washed with water and freeze dried to give the title compound (56mg, 16%). H NMR (DMSO): δ 1.22-1.32 (2H,.m), 1.40-1.57 (4H, m), 1.96 (2H, t), 3.10 (2H, q), 6.25 (IH, t), 7.44-7.53 (2H, m), 7.74-7.82 (2H, m), 8.05 (IH, d), 8.24 (IH, s), 8.54 (IH, s), 8.69 (IH, s), 8.81 (IH, s), 10.36 (IH, s). MS (APCI-) m/z 448.9.
Example 7:
6-[3-[3-(6-Fluorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
(a) 2-(3 -Nitrophenyl)-6-fluorobenzoxazole
Figure imgf000023_0001
Prepared by the method of Example 2a) from 2-amino-5-fluorophenol (309mg, 2.43mmol) and 3- nitrobenzoyl chloride (500mg, 2.69mmol) the subtitle compound was obtained, (573mg, 91%). *H NMR (DMSO): δ 7.35 (IH, dt), 7.86-7.95 (3H, m), 8.47 (IH, m), 8.57 (IH, d), 8.83 (IH, m). (b) 2-(3 - Aminophenyl)-6-fluorobenzoxazole
Figure imgf000023_0002
Prepared by the method of Example 2b) from 2-(3-nitrophenyl)-6-fluorobenzoxazole (572mg, 2.22mmol) the subtitle compound was obtained, (483mg, 96%). !H NMR (DMSO): δ 5.51 (2H, bs), 6.79 (IH, m), 7.20-7.32 (3H, m), 7.40 (IH, t), 7.75-7.82 (2H, m). (c) 6-[3-[3-(6-Fluorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000023_0003
Prepared by the method of Example 2c) from 2-(3-aminophenyl)-6-fluorobenzoxazole (483mg, 2.12mmol) and ethyl 6-isocyanatohexanoate (0.57ml, 3.18mmol) the subtitle compound was obtained, (660mg, 75%). Η NMR (DMSO): δ 1.17 (3H, t), 1.27-1.35 (2H, m), 1.41-1.60 (4H, m), 2.30 (2H, t), 3.10 (2H, q), 4.05 (2H, q), 6.24 (IH, t), 7.26-7.33 (IH, m), 7.41-7.49 (2H, m), 7.68-7.71 (IH, m), 7.80-7.85 (2H, m), 8.47 (IH, s), 8.78 (IH, s). (d) 6-[3-[3-(6-Fluorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid
Figure imgf000024_0001
Prepared by the method of Example 2d) from 6-[3-[3-(6-fluorobenzoxazole-2- yl)phenyl]ureido]hexanoic acid ethyl ester (660mg, 1.60mmol) and lithium hydroxide (191mg, 7.97mmol) the subtitle compound was obtained, (611mg, 99%). 'H NMR (DMSO): δ 1.26-1.35 (2H, m), 1.41-1.58 (4H, ), 2.22 (2H, t), 3.10 (2H, q), 6.32 (IH, t), 7.26-7.33 (IH, m), 7.41-7.50 (2H, m), 7.70 (IH, d), 7.81-7.85 (2H, m), 8.47 (IH, s), 8.88 (IH, s), 12.03 (IH, bs). e) 6-[3-[3-(6-Fluorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
NH NH
HO-N H Prepared by the method of Example 2e) from 6-[3-[3-(6-fluorobenzoxazole-2- yl)phenyl]ureido]hexanoic acid (291mg, 0.76mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (225.3mg, 1.28mmol), N-methyl morpholine (0.25ml, 2.27mmol) and tert-butyldimethylsilyl hydroxylamine. The combined organic extracts were washed with saturated sodium hydrogen carbonate solution (2x50ml) and then stirred vigorously with 2M hydrochloric acid for 2.5h. The resultant precipitate was collected by filtration, washed with water and freeze dried to give the title compound (79mg, 26%). H NMR
(DMSO): δ 1.22-1.31 (2H, m), 1.40-1.57 (4H, m), 1.96 (2H, t), 3.09 (2H, q), 6.24 (IH, t), 7.26-7.33 (IH, m), 7.42-7.49 (2H, m), 7.70 (IH, dt), 7.81-7.85 (2H, m), 8.47 (IH, s), 8.69 (IH, s), 8.78 (IH, s), 10.36 (IH, s). MS (APCI-) m/z 399.0.
Example 8:
6- [3- [2-Methoxy-5-(benzoxazole-2-yl)phenyl] -3-(methyl)ur eido] hexanoic acid hydroxyamide
(a) 2-(3 -N-Methylamino-4-methoxyphenyl)benzoxazole
Figure imgf000024_0002
To a stirred solution of 2-(3-amino-4-methoxyphenyl)benzoxazole (500mg, 2.08mmol) in anhydrous dimethylformamide (3ml) was added N,N-dimethylformamide dimethyl acetal (1.52ml, 11.45mmol). The reaction was stirred at 40°C for lh. After allowing to cool to room temperature, sodium borohydride (552mg, 14.6mmol) was added and the reaction was stirred overnight. Sodium borohydride (276mg, 7.28mmol) was added and the reaction was heated at 50°C overnight. The mixture was partitioned between ethyl acetate (100ml) and saturated sodium hydrogen carbonate solution (50ml). The organic layer was washed with water (3x50ml), saturated sodium chloride solution (50ml), dried (Na2S04) and concentrated. The crude compound was subjected to flash silica gel column chromatography using 1%> methanol-dichloromethane as eluant to give the subtitle compound (247mg, 47%). Η NMR (DMSO): δ 2.81 (3H, d), 3.88 (3H, s), 5.44 (IH, q), 7.00 (IH, d), 7.21 (IH, d), 7.34-7.40 (2H, m), 7.47 (IH, dd), 7.72-7.77 (2H, m). (b) 6-[3-[2-Methoxy-5-(benzoxazole-2-yl)phenyl]-3-(methyl)ureido]hexanoic acid ethyl ester
Figure imgf000025_0001
Prepared by the method of Example 2c) from 2-(3-N-methylamino-4-methoxyphenyl)- benzoxazole (240mg, 0.94mmol) and ethyl 6-isocyanatohexanoate (0.25ml, 1.42mmol) the subtitle compound was obtained, (186mg, 45%). 'H ΝMR (DMSO): δ 1.12-1.24 (5H, m), 1.30-1.40 (2H, m), 1.44-1.54 (2H, m), 2.24 (2H, t), 2.95 (2H, q), 3.04 (3H, s), 3.89 (3H, s), 4.02 (2H, q), 5.95 (IH, t), 7.34 (IH, d), 7.37-7.43 (2H, m), 7.75-7.78 (2H, m), 7.94 (IH, d), 8.14 (IH, dd). (c) 6-[3-[2-Methoxy-5-(benzoxazole-2-yl)phenyl]-3-(methyl)ureido]hexanoic acid
Figure imgf000025_0002
Prepared by the method of Example 2d) from 6-[3-[2-methoxy-5-(benzoxazole-2-yl)phenyl]-3- (methyl)ureido]hexanoic acid ethyl ester (185mg, 0.42mmol) and lithium hydroxide (50mg, 2.1 lmmol) the subtitle compound was obtained, (141mg, 81%). Η MR (DMSO): δ 1.14-1.24 (2H, m), 1.30-1.40 (2H, m), 1.42-1.52 (2H, m), 2.17 (2H, t), 2.95 (2H, q), 3.04 (3H, s), 3.89 (3H, s), 5.96 (IH, t), 7.34 (IH, d), 7.36-7.43 (2H, m), 7.76-7.78 (2H, m), 7.94 (IH, d), 8.14 (IH, dd), 11.98 (IH, bs). (d) 6-[3-[2-Methoxy-5-(benzoxazole-2-yl)phenyl]-3-(methyl)ureido]hexanoic acid hydroxyamide
Figure imgf000026_0001
Prepared by the method of Example 2e) from 6-[3-[2-methoxy-5-(benzoxazole-2-yl)phenyl]-3- (methyl)ureido]hexanoic acid (140mg, 0.34mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (102mg, 0.58mmol), N-methylmorpholine (0.11ml, 1.02mmol) and tert-butyldimethylsilyl hydroxylamine. The combined organic extracts were washed with saturated sodium hydrogen carbonate solution (2x50ml) and then stirred vigorously with 2M hydrochloric acid for 20min. The organic layer was washed with brine, dried (Na2S04) and concentrated until a precipitate formed. The solid was collected by filtration, washed with ethyl acetate and dried under vacuum to give the title compound (30mg, 21%). JH NMR (DMSO): δ 1.11-1.21 (2H, m), 1.29-1.38 (2H, m), 1.40-1.50 (2H, m), 1.91 (2H, t), 2.94 (2H, q), 3.04 (3H, s), 3.89 (3H, s), 5.95 (IH, t), 7.33 (IH, d), 7.36-7.42 (2H, m), 7.77 (2H, dd), 7.94 (IH, d), 8.14 (IH, m), 8.66 (IH, s), 10.33 (IH, s). MS (APCI+) m/z All .2 MS (APCI-) m/z 425.5.
Example 9:
6-[3-[5-(5-Chlorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid hydroxyamide (a) 5-Chloro-2-(4-methoxy-3-nitrophenyl)benzoxazole
Figure imgf000026_0002
Prepared by the method of Example 2a) from 2-amino-4-chlorophenol (300mg, 2.09mmol) and 4- methoxy-3-nitrobenzoyl chloride (500mg, 2.32mmol) the subtitle compound was obtained, (275mg, 43%). lE NMR (DMSO): δ 4.00 (3H, s), 7.43 (IH, dd), 7.55 (IH, d), 7.77 (IH, d), 7.86 (IH, d), 8.36 (IH, dd), 8.55 (IH, d).
(b) 5-Chloro-2-(3-amino-4-methoxyphenyl)benzoxazole
Figure imgf000026_0003
Prepared by the method of Example 2b) from 5-chloro-2-(4-methoxy-3-nitrophenyl)benzoxazole
(275mg, 0.90mmol) the subtitle compound was obtained, (233mg, 94%). *H NMR (DMSO): δ 3.98 (3H, s), 5.20 (2H, s), 7.06 (IH, d), 7.47 (2H, m), 7.55 (IH, d), 7.84 (IH, d), 7.89 (IH, d).
(c) 6-[3-[5-(5-Chlorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid ethyl ester
Figure imgf000027_0001
Prepared by the method of Example 2c) from 5-chloro-2-(3-amino-4- methoxyphenyl)benzoxazole (233mg, 0.85mmol) and ethyl 6-isocyanatohexanoate (0.23ml, 1.27mmol) the subtitle compound was obtained, (298mg, 76%). !H NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.41-1.48 (2H, m), 1.50-1.60 (2H, m), 2.30 (2H, t), 3.10 (2H, q), 3.96 (3H, s), 4.05 (2H, q), 6.98 (IH, t), 7.20 (IH, d), 7.43 (IH, dd), 7.76 (IH, dd), 7.82 (IH, d), 7.86 (IH, d), 8.15 (IH, s), 9.06 (IH, s). (d) 6-[3 -[5 -(5 -Chlorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid
Figure imgf000027_0002
Prepared by the method of Example 2d) from 6-[3-[5-(5-chlorobenzoxazole-2-yl)-2- methoxyphenyl]ureido]hexanoic acid ethyl ester (282mg, 0.61mmol) and lithium hydroxide (73mg, 3.06mmol) the subtitle compound was obtained, (225mg, 85%). Η NMR (DMSO): δ 1.27-1.35 (2H, m), 1.38-1.58 (4H, m), 2.22 (2H, t), 3.10 (2H, q), 3.96 (3H, s), 6.98 (IH, t), 7.20 (IH, d), 7.32 (IH, dd), 7.76 (IH, dd), 7.81 (IH, d), 7.85 (IH, d), 8.17 (IH, s), 9.06 (IH, d), 12.00 (IH, s). (e) 6-[3-[5-(5-Chlorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid hydroxyamide
aXXXX NH°' NH
HO-N H
Prepared by the method of Example 2e) from 6-[3-[5-(5-chlorobenzoxazole-2-yl)-2- methoxyphenyl]ureido]hexanoic acid (199mg, 0.46mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (137mg, 0.78mmol), N-methylmorpholine (0.15ml, 1.38mmol) and tert-butyldimethylsilyl hydroxylamine (102mg, 0.69mmol) the title compound was obtained (173mg, 84%). Η NMR (DMSO): δ 1.22-1.32 (2H, m), 1.40-1.57 (4H, m), 1.96 (2H, t), 3.10 (2H, bs), 3.98 (3H, s), 7.05 (IH, bs), 7.24 (IH, d), 7.47 (IH, dd), 7.81 (IH, dd), 7.86 (IH, d), 7.91 (IH, d), 8.20 (IH, s), 9.11 (IH, s), 10.42 (IH, bs). MS (APCI+) m/z 447, (APCI-) m/z 445.
Example 10: 6- [3- [5-(5-FIuorobenzoxazoIe-2-yl)-2-methoxyphenyl] ureido] hexanoic acid hydroxyamide
(a) 5-Fluoro-2-(4-methoxy-3 -nitrophenyl)benzoxazole
Figure imgf000028_0001
Prepared by the method of Example 2a) from 2-amino-4-fluoroρhenol (266mg, 2.09mmol) and 4- methoxy-3-nitrobenzoyl chloride (500mg, 2.32mmol) the subtitle compound was obtained, (386mg, 64%). !H NMR (DMSO): δ 4.01 (3H, s), 7.31 (IH, dt), 7.60 (IH, d), 7.70 (IH, dd), 7.84 (IH, dd), 8.43 (IH, dd), 8.60 (IH, d).
(b) 5-Fluoro-2~(3-amino-4-methoxyphenyl)benzoxazole
FXXxXX NH°2'
Prepared by the method of Example 2b) from 5-fluoro-2-(4-methoxy-3-nitτophenyl)benzoxazole (386mg, 1.34mmol) the subtitle compound was obtained, (297mg, 86%). Η NMR (DMSO): δ 3.80 (3H, s), 5.08 (2H, s), 6.90 (IH, d), 7.12 (IH, dt), 7.32 (IH, dd), 7.39 (IH, d), 7.50 (IH, dd), 7.67 (IH, dd). (c) 6-[3-[5-(5-Fluorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid ethyl ester
Figure imgf000028_0002
Prepared by the method of Example 2c) from 5-fluoro-2-(3-amino-4-methoxyphenyl)benzoxazole (297mg, 1.15mmol) and ethyl 6-isocyanatohexanoate (0.31ml, 1.72mmol) the subtitle compound was obtained, (379mg, 74%). Η NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.41-1.48 (2H, m), 1.50- 1.60 (2H, m), 2.30 (2H, t), 3.10 (2H, q), 3.96 (3H, s), 4.05 (2H, q), 6.98 (IH, t), 7.20 (IH, d), 7.23 (IH, dt), 7.62 (IH, dd), 7.78 (IH, dd), 7.80 (IH, dd), 8.15 (IH, s), 9.06 (IH, s). (d) 6-[3 -[5 -(5 -Fluorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid
Figure imgf000028_0003
Prepared by the method of Example 2d) from 6-[3-[5-(5-fluorobenzoxazole-2-yl)-2- methoxyphenyl]ureido]hexanoic acid ethyl ester (326mg, 0.73mmol) and lithium hydroxide (88mg, 3.67mmol) the subtitle compound was obtained, (247mg, 81%). *H NMR (DMSO): δ 1.25-1.35 (2H, m), 1.38-1.58 (4H, m), 2.21 (2H, t), 3.10 (2H, q), 3.97 (3H, s), 6.98 (IH, t), 7.20 (IH, d), 7.23 (IH, dt), 7.62
(IH, dd), 7.78 (IH, dd), 7.80 (IH, dd), 8.15 (IH, s), 9.06 (IH, d), 12.01 (IH, s).
(e) 6-[3-[5-(5-Fluorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000029_0001
Prepared by the method of Example 2e) from 6-[3-[5-(5-fluorobenzoxazole-2-yl)-2- methoxyphenyl]ureido]hexanoic acid (230mg, 0.55mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (165mg, 0.94mmol), N-methylmorpholine (0.18ml, 1.66mmol) and tert-butyldimethylsilyl hydroxylamine (122mg, 0.83mmol) the title compound was obtained (130mg, 54%). lH NMR (DMSO): δ 1.22-1.30 (2H, m), 1.37-1.53 (4H, m), 1.94 (2H, t), 3.08 (2H, bs), 3.95 (3H, s), 6.95 (IH, t), 7.15 (IH, d), 7.22 (IH, dt), 7.60 (IH, dd), 7.72 (IH, dd), 7.77 (IH, dd), 8.10 (IH, s), 9.03 (IH, s), 10.32 (IH, bs). MS (APCI+) m/z 431, (APCI-) m/z 429.
Example 11: 6-[3-[2-Methoxy-5-[5-(trifluoromethyl)benzoxazole-2-yI]phenyl]ureido]hexanoic acid hydroxyamide
(a) 5-Trifluoromethyl-2-(4-methoxy-3-nitrophenyl)benzoxazole
Figure imgf000029_0002
Prepared by the method of Example 2a) from 2-amino-4-(trifluoromethyl)phenol (370mg,
2.09mmol) and 4-methoxy-3-nitrobenzoyl chloride (500mg, 2.32mmol) the subtitle compound was obtained, (371mg, 52%). Η NMR (DMSO): δ 4.05 (3H, s), 7.62 (IH, d), 7.80 (IH, d), 8.02 (IH, d), 8.20 (IH, s), 8.43 (IH, dd), 8.62 (IH, s).
(b) 5-Trifluoromethyl-2-(3 -amino-4-methoxyphenyl)benzoxazole
Figure imgf000029_0003
Prepared by the method of Example 2b) from 5-trifluoromethyl-2-(4-methoxy-3-nitrophenyl)- benzoxazole (370mg, 1.09mmol) the subtitle compound was obtained, (325mg, 97%). 'H MR (DMSO): δ 3.90 (3H, s), 5.20 (2H, s), 7.02 (IH, d), 7.47 (IH, dd), 7.55 (IH, d), 7.75 (IH, dd), 7.96 (IH, d), 8.15 (lH, s).
(c) 6-[3-[2-Methoxy-5-[5-(trifluoromethyl)benzoxazole-2-yl]phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000030_0001
Prepared by the method of Example 2c) from 5-trifluoromethyl-2-(3-amino-4-methoxyphenyl)- benzoxazole (325mg, 1.06mmol) and ethyl 6-isocyanatohexanoate (0.28ml, 1.58mmol) the subtitle compound was obtained, (425mg, 81%). *H NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.41-1.48 (2H, m), 1.50-1.60 (2H, m), 2.30 (2H, t), 3.10 (2H, q), 3.96 (3H, s), 4.05 (2H, q), 6.98 (IH, t), 7.20 (IH, d), 7.76 (IH, d), 7.81 (IH, dd), 8.01 (IH, d), 8.16 (IH, d), 9.10 (IH, s). (d) 6-[3-[2-Methoxy-5-[5-(trifluoromethyl)benzoxazole-2-yl]phenyl]ureido]hexanoic acid
Figure imgf000030_0002
Prepared by the method of Example 2d) from 6-[3-[2-methoxy-5-[5- (trifluoromethyl)benzoxazole-2-yl]phenyl]ureido]hexanoic acid ethyl ester (410mg, 0.83mmol) and lithium hydroxide (99mg, 4.16mmol) the subtitle compound was obtained, (349mg, 90%). *H NMR (DMSO): δ 1.25-1.35 (2H, m), 1.38-1.58 (4H, m), 2.22 (2H, t), 3.11 (2H, q), 3.97 (3H, s), 6.98 (IH, t), 7.21 (IH, d), 7.75 (IH, dd), 7.82 (IH, dd), 8.01 (IH, d), 8.18 (IH, d), 9.10 (IH, d), 12.02 (IH, s). (e) 6-[3 -[2-Methoxy-5 -[5 -(trifluoromethyl)benzoxazole-2-yl]phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000030_0003
Prepared by the method of Example 2e) from 6-[3-[2-methoxy-5-[5-(trifluoromethyl)- benzoxazole-2-yl]phenyl]ureido]hexanoic acid (337mg, 0.73mmol), 2-chloro-4,6-dimethoxy-l,3,5- triazine (216mg, 1.23mmol), N-methylmorpholine (0.24ml, 2.18mmol) and tert-butyldimethylsilyl hydroxylamine (160mg, 1.09mmol) the title compound was obtained (195mg, 56%). *H NMR (DMSO): δ 1.23-1.32 (2H, ), 1.37-1.56 (4H, m), 1.96 (2H, t), 3.10 (2H, q), 3.97 (3H, s), 6.98 (IH, t), 7.22 (IH, d), 7.77 (IH, dd), 7.80 (IH, dd), 8.01 (IH, d), 8.18 (IH, s), 8.68 (IH, s), 9.10 (IH, d), 10.36 (IH, bs). MS (APCI+) m/z 481, (APCI-) m/z 478.
Example 12: 6-[3-[2-Methoxy-5-(5-methoxybenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
(a) 5-Methoxy-2-(4-methoxy-3-nitrophenyl)benzoxazole
Figure imgf000031_0001
Prepared by the method of Example 2a) from 2-amino-4-methoxyphenol (291mg, 2.09mmol) and 4-methoxy-3-nitrobenzoyl chloride (500mg, 2.32mmol) the subtitle compound was obtained, (189mg, 30%). Η NMR (DMSO): δ 3.82 (3H, s), 4.05 (3H, s), 7.02 (IH, dd), 7.36 (IH, d), 7.60 (IH, d), 7.70 (IH, d), 8.40 (IH, dd), 8.58 (IH, d). (b) 2-(3-Amino-4-methoxyphenyl)-5-methoxybenzoxazole
Figure imgf000031_0002
Prepared by the method of Example 2b) from 5-methoxy-2-(4-methoxy-3-nitrophenyl)benzoxazole (189mg, 0.63mmol) the subtitle compound was obtained, (148mg, 87%). 'H NMR (DMSO): δ 3.85 (3H, s), 3.90 (3H, s), 5.12 (2H, s), 6.95 (IH, dd), 6.98 (IH, d), 7.28 (IH, d), 7.40 (IH, dd), 7.47 (IH, d), 7.62 (IH, d). (c) 6-[3-[2-Methoxy-5-(5-methoxybenzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000031_0003
Prepared by the method of Example 2c) from 2-(3-amino-4-methoxyphenyl)-5- methoxybenzoxazole (148mg, 0.55mmol) and ethyl 6-isocyanatohexanoate (0.15ml, 0.82mmol) the subtitle compound was obtained, (48mg, 19%). Η NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.41-1.48 (2H, m), 1.50-1.60 (2H, m), 2.30 (2H, t), 3.10 (2H, q), 3.82 (3H, s), 3.94 (3H, s), 4.05 (2H, q), 6.98 (IH, t), 7.18 (IH, d), 7.32 (IH, d), 7.65 (IH, d), 7.72 (IH, dd), 8.10 (IH, s), 9.03 (IH, d). (d) 6-[3-[2-Methoxy-5-(5-methoxybenzoxazole-2-yl)phenyl]ureido]hexanoic acid
Figure imgf000032_0001
Prepared by the method of Example 2d) from 6-[3-[2-methoxy-5-(5-methoxybenzoxazole-2- yl)phenyl]ureido]hexanoic acid ethyl ester (42mg, 0.09mmol) and lithium hydroxide (1 lmg, 0.46mmol) the subtitle compound was obtained, (34mg, 85%). 'HNMR (DMSO): δ 1.25-1.35 (2H, m), 1.38-1.58 (4H, ), 2.22 (2H, t), 3.10 (2H, q), 3.82 (3H, s), 3.96 (3H, s), 6.98 (IH, dd), 7.17 (IH, d), 7.31 (IH, d), 7.66 (IH, d), 7.73 (IH, dd), 8.12 (IH, s), 9.02 (IH, d), 12.02 (IH, bs). (e) 6-[3-[2-Methoxy-5-(5-methoxybenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000032_0002
Prepared by the method of Example 2e) from 6-[3-[2-methoxy-5-(5-methoxybenzoxazole-2- yl)ρhenyl]ureido]hexanoic acid (23mg, 0.05mrnoi), 2-chloro-4,6-dimethoxy-l,3,5-triazine (16.3mg, 0.09mmol), N-methylmorpholine (0.02ml, O.lό mol) and tert-butyldimethylsilyl hydroxylamine (12.1mg, 0.08mmol) the title compound was obtained (16mg, 66%>). !H NMR (DMSO): δ 1.23-1.32 (2H, m), 1.37-1.56 (4H, m), 1.96 (2H, t), 3.10 (2H, q), 3.82 (3H, s), 3.97 (3H, s), 6.98 (IH, dd), 7.17 (IH, d), 7.31 (IH, d), 7.65 (IH, d), 7.74 (IH, dd), 8.13 (IH, s), 9.04 (IH, s), 10.39 (IH, s). MS (APCI+) m/z 443, (APCI-) rø/z 441.
Example 13:
6-[3-[2-Methoxy-5-(5,7-dichlorobenzoxazole-2-yI)phenyI]ureido]hexanoic acid hydroxyamide (a) 5,7-Dichloro-2-(4-methoxy-3-nitrophenyl)benzoxazole
Figure imgf000032_0003
Prepared by the method of Example 2a) from 2-amino-4, 6-dichlorophenol (372mg, 2.09mmol) and 4-methoxy-3-nitrobenzoyl chloride (500mg, 2.32mmol) the subtitle compound was obtained, (376mg, 53%). *H NMR (DMSO): δ 4.05 (3H, s), 7.62 (IH, d), 7.72 (IH, d), 7.94 (IH, d), 8.42 (IH, dd), 8.61 (IH, d). (b) 2-(3 -Amino-4-methoxyphenyl)-5 ,7-dichlorobenzoxazole
Figure imgf000033_0001
Prepared by the method of Example 2b) from 5,7-dichloro-2-(4-methoxy-3- nitrophenyl)benzoxazole (376mg, l.l lmmol) the subtitle compound was obtained, (245mg, 71%). !H NMR (DMSO): δ 3.90 (3H, s), 5.20 (2H, s), 7.02 (IH, d), 7.45 (IH, dd), 7.50 (IH, d), 7.65 (IH, d), 7.87 (lH, d). (c) 6-[3 -[2-Methoxy-5 -(5,7 -dichlorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000033_0002
Prepared by the method of Example 2c) from 2-(3-amino-4~methoxyphenyl)-5,7- dichlorobenzoxazole (245mg, 0.79mmol) and ethyl 6-isocyanatohexanoate (0.21ml, 1.19mmol) the subtitle compound was obtained, (377mg, 97%). !H NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.41-1.48 (2H, m), 1.50-1.61 (2H, m), 2.30 (2H, t), 3.10 (2H, q), 3.96 (3H, s), 4.05 (2H, q), 6.98 (IH, t), 7.21 (IH, d), 7.66 (IH, d), 7.77 (IH, dd), 7.88 (IH, d), 8.16 (IH, s), 9.07 (IH, s). (d) 6-[3-[2-Methoxy-5-[5,7-dichlorobenzoxazole-2-yl]phenyl]ureido]hexanoic acid
Figure imgf000033_0003
Prepared by the method of Example 2d) from 6-[3-[2-methoxy-5-(5,7-dichlorobenzoxazole-2- yl)phenyl]ureido]hexanoic acid ethyl ester (369mg, 0.75mmol) and lithium hydroxide (89mg, 3.73mmol) the subtitle compound was obtained, (306mg, 88%). TI MR (DMSO): δ 1.25-1.35 (2H, m), 1.38-1.58 (4H, m), 2.25 (2H, t), 3.11 (2H, q), 3.98 (3H, s), 6.98 (IH, t), 7.21 (IH, d), 7.68 (IH, d), 7.78 (IH, dd), 7.90 (IH, d), 8.18 (IH, s), 9.07 (IH, d), 12.02 (IH, s). (e) 6-[3-[2-Methoxy-5-(5,7-dichlorobenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000034_0001
Prepared by the method of Example 2e) from 6-[3-[2-methoxy-5-(5,7-dichlorobenzoxazole-2-yl)- phenyl]ureido]hexanoic acid (289mg, 0.62mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (185mg, 1.05mmol), N-methylmorpholine (0.20ml, 1.86mmol) and tert-butyldimethylsilyl hydroxylamine (137mg, 0.93mmol) the title compound was obtained (201mg, 67%). Η NMR (DMSO): δ 1.23-1.32 (2H, m), 1.37-1.56 (4H, ), 1.97 (2H, t), 3.10 (2H, q), 3.97 (3H, s), 6.98 (IH, t), 7.22 (IH, d), 7.66 (IH, d), 7.77 (IH, dd), 7.89 (IH, d), 8.18 (IH, s), 8.68 (IH, bs), 9.07 (IH, s), 10.36 (IH, bs). MS (APCI+) m/z 481, (APCI-) m z 479.
Example 14:
6-[3-[5-(6-Fluorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid hydroxyamide
(a) 6-Fluoro-2-(4-methoxy-3 -nitrophenyl)benzoxazole
Figure imgf000034_0002
Prepared by the method of Example 2a) from 2-amino-5-fluorophenol (266mg, 2.09mmol) and 4- methoxy-3-nitrobenzoyl chloride (500mg, 2.32mmol) the subtitle compound was obtained, (486mg,
81%). !H NMR (DMSO): δ 4.05 (3H, s), 7.32 (IH, dt), 7.62 (IH, d), 7.79-7.87 (2H, m), 8.41 (IH, dd),
8.59 (IH, d). (b) 2-(3-Amino-4-methoxyρhenyl)-6-fluorobenzoxazole
Figure imgf000034_0003
Prepared by the method of Example 2b) from 6-fluoro-2-(4-methoxy-3-nitrophenyl)benzoxazole (486mg, 1.69mmol) the subtitle compound was obtained, (352mg, 81%). XH NMR (DMSO): δ 3.89 (3H, s), 5.12 (2H, s), 7.00 (IH, d), 7.24 (IH, m), 7.39 (IH, dd), 7.47 (IH, d), 7.74 (2H, m).
(c) 6-[3-[5-(6-Fluorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid ethyl ester
Figure imgf000035_0001
Prepared by the method of Example 2c) from 2-(3-amino-4-methoxyphenyl)-6-fluorobenzoxazole (352mg, 1.37mmol) and ethyl 6-isocyanatohexanoate (0.37ml, 2.06mmol) the subtitle compound was obtained, (452mg, 74%). Η NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.40-1.48 (2H, m), 1.50- 1.61 (2H, m), 2.30 (2H, t), 3.10 (2H, q), 3.94 (3H, s), 4.05 (2H, q), 6.98 (IH, t), 7.19 (IH, d), 7.26 (IH, m), 7.76 (3H, m), 8.13 (IH, s), 9.04 (IH, s). (d) 6-[3-[5-(6-Fluorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid
Figure imgf000035_0002
Prepared by the method of Example 2d) from 6-[3-[5-(6-fluorobenzoxazole-2-yl)-2- methoxyphenyl]ureido]hexanoic acid ethylester (447mg, l.Olmmol) and lithium hydroxide (121mg, 5.03mmol) the subtitle compound was obtained, (340mg, 81%). !H NMR (DMSO): δ 1.25-1.35 (2H, m), 1.38-1.58 (4H, m), 2.22 (2H, t), 3.11 (2H, q), 3.96 (3H, s), 6.98 (IH, t), 7.19 (IH, d), 7.27 (2H, m), 7.76 (3H, m), 8.16 (IH, s), 9.04 (IH, d), 12.02 (IH, s). (e) 6-[3-[5-(6-Fluorobenzoxazole-2-yl)-2-methoxyphenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000035_0003
Prepared by the method of Example 2e) from 6-[3-[5-(6-fluorobenzoxazole-2~yl)-2- methoxyphenyι]ureido]hexanoic acid (316mg, 0.76mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (227mg, 1.29mmol), N-methyl morpholine (0.25ml, 2.28mmol) and tert-butyl dimethylsilyl hydroxylamine (168mg, 1.14mmol) the title compound was obtained (250mg, 76%>). !H NMR (DMSO): δ 1.23-1.32 (2H, m), 1.37-1.56 (4H, m), 1.97 (2H, t), 3.10 (2H, bs), 3.96 (3H, s), 6.98 (IH, t), 7.18 (IH, d), 7.26 (2H, m), 7.76 (3H, m), 8.14 (IH, s), 9.04 (IH, s), 10.37 (IH, bs). MS (APCI+) m/z 431, (APCI-) m/z 430. Example 15:
6- [3- [3-(5-Phenylbenzoxazole-2-yl)phenylJ ureido] hexanoic acid hydroxyamide
(a) 2-(3 -Nitrophenyl)-5 -phenylbenzoxazole
Figure imgf000036_0001
Prepared by the method of Example 2a) from 2-amino-4-phenylphenol (5.00g, 26.99mmol) and 3-nitrobenzoyl chloride (5.00g, 26.99mmol) the subtitle compound was obtained, (7.90g, 93%>). *H NMR (DMSO): δ 7.42 (IH, q), 7.52 (2H, t), 7.78 (3H, m), 7.93 (2H, m), 8.12 (IH, s), 8.49 (IH, d), 8.63 (IH, d), 8.89 (IH, s). (b) 2-(3 -Aminopheny l)-5 -phenylbenzoxazole
Figure imgf000036_0002
Prepared by the method of Example 2b) from 2-(3-nitrophenyl)-5-phenylbenzoxazole (7.90g, 24.97mmol) the subtitle compound was obtained, (6.36g, 89%). XH NMR (DMSO): δ 5.51 (2H, s), 6.81 (IH, d), 7.25 (IH, t), 7.38 (2H, m), 7.49 (3H, m), 7.72 (3H, m), 7.85 (IH, d), 8.04 (IH, d). (c) 6-[3-[3-(5-Phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid ethyl ester
Figure imgf000036_0003
Prepared by the method of Example 2c) from 2-(3-aminophenyl)-5 -phenylbenzoxazole (500mg, 1.75mmol) and ethyl 6-isocyanatohexanoate (0.47ml, 2.62mmol) the subtitie compound was obtained, (670mg, 82%). !H NMR (DMSO): δ 1.17 (3H, t), 1.25-1.35 (2H, m), 1.38-1.58 (4H, m), 2.30 (2H, t), 3.10 (2H, q), 4.05 (2H, q), 6.25 (IH, t), 7.36-7.56 (5H, m), 7.71-7.78 (4H, m), 7.88 (IH, d), 8.07 (IH, s), 8.52 (IH, s), 8.80 (IH, s). (d) 6-[3-[3-(5-Phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid
Figure imgf000036_0004
Prepared by the method of Example 2d) from 6-[3-[3-(5-phenylbenzoxazole-2- yi)phenyl]ureido]hexanoic acid ethyl ester (670mg, 1.42mmol) and lithium hydroxide (170mg, 7.10mmol) the subtitle compound was obtained, (570mg, 90%). Η NMR (DMSO): δ 1.25-1.35 (2H, m), 1.38-1.58 (4H, m), 2.22 (2H, t), 3.11 (2H, q), 6.25 (IH, t), 7.37-7.55 (5H, m), 7.70-7.78 (4H, m), 7.89 (IH, d), 8.08 (IH, s), 8.52 (IH, s), 8.80 (IH, s), 12.03 (IH, s). (e) 6-[3-[3-(5-Phenylbenzoxazole-2-yl)phenyl]ureido]hexanoic acid hydroxyamide
Figure imgf000037_0001
Prepared by the method of Example 2e) from 6-[3-[3-(5-phenylbenzoxazole-2- yl)phenyl]ureido]hexanoic acid (500mg, 1.13mmol), 2-chloro-4,6-dimethoxy-l,3,5-triazine (337mg, 1.92mmol), N-methyl morpholine (0.37ml, 3.38mmol) and tert-butyl dimethylsilyl hydroxylamine (249mg, 1.69mmol) the title compound was obtained (120mg, 23%). Η NMR (DMSO): δ 1.25-1.35
(2H, m), 1.38-1.57 (4H, m), 1.97 (2H, t), 3.10 (2H, q), 6.24 (IH, t), 7.37-7.57 (5H, m), 7.70-7.78 (4H, m), 7.88 (IH, d), 8.07 (IH, s), 8.52 (IH, s), 8.68 (IH, s), 8.81 (IH, s), 10.36 (IH, s). MS (APCI+) m/z 459, (APCI-) m/z 457.
Biological Data
Histone Deacetylase assay
A histone deacetylase assay kit was purchased from BIOMOL® Research Laboratories, AK-500, and the manufacturers protocol was followed.
The assay is based upon a substrate, which has an acetylated lysine chain. Deacetylation of the substrate, by histone deacetylase enzyme, sensitises the substrate to the developer producing a fiuorophore that can be measured in a fiuorometric plate reader.
The assays were performed according to the BIOMOL® protocol and the source of the histone deacetylases was HeLa nuclear extract. Components added to the wells were as follows, assay buffer (final assay volume 50μl), inhibitors (lOμl), histone deacetylase source (HeLa extract, 15μl containing ~4μg), substrate (25 μl, 1/200 dilution stock). The wells were mixed and the assay incubated for 30 min at R.T. before taking readings using a Nictor2 fiuorometric plate reader (excitation at 350nM and emission detected at 440nm).
The following table gives the histone deacetylase activity of representative compounds of the invention.
Figure imgf000037_0002
Figure imgf000038_0001
Cell Cycle Analysis and Acetylation of Histones
The compound of Example 1 was assessed for its effect on cancerous cell growth. Cell cycle analysis was performed on HL60 (human promyelocytic leukaemia cell line) cells by using BrdU incorporation and flow cytometry, methods that are well known to those skilled in the art. The compound of Example 1 was found to cause a dose dependant accumulation of cells in the G2M phase of the cell cycle, thus arresting the cells prior to mitosis.
Acetylation of histones by the compound of Example 1 was investigated using the methods described in Richon et al., (2000), PNAS, vol 98, No 18. This method is based on assessment of acetylated histone protein using a rabbit anti-acetylated histone H4 antibody and Western Blotting techniques, methods that are well known to those skilled in the art. The compound of Example 1 was found to cause a dose dependant increase in histone acetylation in MCF7 (human mammary epithelial cell line) cells.

Claims

CLAIMS:
1. A compound of formula (I) or a pharmaceutically acceptable salt or prodrug thereof:
Figure imgf000039_0001
(D wherein
R1, R2 and R3 are independently, hydrogen, halogen, CF3, OR6, NR7R8, NR8COR10, NR8S02R10 or Q_6 alkyl optionally substitated by hydroxyl or Q.6 alkoxy; R4 is R8CONR8R9:
R5 is
Figure imgf000039_0002
wherein one of X and Y is R11 and the other is hydrogen or halogen; or X and Y together with the carbon atoms to which they are attached form a fused six-membered aromatic ring; Z is NR8, O or S;
R6 is hydrogen or Q.6 alkyl, Q_6 alkenyl or C3.6 alkynyl any of which can optionally be substitated by hydroxyl, Q.6 alkoxy or NR7R8;
R7 is hydrogen or Q_6 alkyl or C3.6 alkenyl either of which can optionally be substitated by Q_6 alkoxy;
R8 is hydrogen or Q.6 alkyl; or the groups R7 and R8 may together with the nitrogen to which they are attached form a 5- or 6- membered ring which optionally contains up to two further heteroatoms selected from NR8, S and O;
R9 is Q.io alkyl or C3_ι0 alkenyl wherein a -CH2- group other than that adjacent to the N may be replaced by -O- and wherein the alkyl or alkenyl is substitated by one or more hydroxamic acid groups (CONHOH); or in R4 the groups R8 and R9 may together with the nitrogen to which they are attached form a 5- or 6-membered ring, which is substitated with one or more hydroxamic acid groups (CONHOH);
R10 is Q.6 alkyl; and
Rπ is hydrogen, halogen, Q.6 alkyl, CF3, OCF3, CN, OR6 or phenyl optionally substitated by one or more substituents selected from halogen, Q.6 alkyl, CF3, OCF3, OR6, CN and methylenedioxo; or a 5- to 10-membered heteroaryl group containing up to three heteroatoms selected from O, N and S, which heteroaryl group may optionally be substitated by one or more substituents selected from Q.6 alkyl, Q.6 alkoxy and halogen.
2. A compound according to claim 1 where R1, R2 and R3 are independently, hydrogen, halogen, OR6, NR7R8, or Q.6 alkyl optionally substitated by hydroxyl or Q.6 alkoxy.
3. A compound according to any one of the preceding claims where Z is O.
4. A compound according to any one of the preceding claims where R6 is hydrogen or Q_6 alkyl, C3.6 alkenyl or C3.6 alkynyl any of which can optionally be substitated by hydroxyl or Q.6 alkoxy.
5. A compound according to any one of the preceding claims where R11 is hydrogen, halogen, Q_6 alkyl, CF3, OCF3, CN, OR6 or phenyl.
6. A compound according to any one of the preceding claims where R1 is hydrogen, OR6 or NR7R8.
7. A compound according to any one of the preceding claims where R2 is hydrogen.
8. A compound according to any one of the preceding claims where R3 is hydrogen, halogen or OR6
9. A compound according to any one of the preceding claims where the configuration of the R groups is:
Figure imgf000040_0001
10. A compound of formula (I) as described in any one of Examples 1 to 15 or a pharmaceutically acceptable salt or prodrug thereof.
11. A compound as defined in any one of claims 1 to 10 for use in medicine.
12. A pharmaceutical composition comprising a compound according to any one of claims 1 to 10 together with a pharmaceutically acceptable carrier or excipient.
13. The use of a compound as defined in any one of claims 1 to 10 in the manufacture of a medicament for the inhibition of histone deacetylase enzyme activity.
14. The use of a compound as defined in any one of claims 1 to 10 in the manufacture of a medicament for the treatment of a subject with cancer.
15. The use of a compound as defined in any one of claims 1 to 10, in the manufacture of a medicament for the treatment of a disease selected from angiogenesis or an angiogenesis dependent disease, an inflammatory disease and a proliferative skin disorder.
16. A compound as defined in any one of claims 1 to 10, wherein the one or more hydroxamic acid groups (CONHOH) are replaced by CONHOP wherein P is a protecting group.
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US7732475B2 (en) 2005-07-14 2010-06-08 Takeda San Diego, Inc. Histone deacetylase inhibitors
US7741494B2 (en) 2005-07-14 2010-06-22 Takeda San Diego, Inc. Histone deacetylase inhibitors
JP2009508811A (en) * 2005-08-04 2009-03-05 サートリス ファーマシューティカルズ， インコーポレイテッド Oxazolopyridine derivatives as sirtuin modulators
US8283015B2 (en) 2006-09-29 2012-10-09 Sabic Innovative Plastics Ip B.V. Authenticatable articles and methods therefor
CN110790686A (en) * 2019-10-08 2020-02-14 南开大学 Compound and application thereof in preparing anti-inflammatory and acute lung injury, chronic obstructive lung disease, asthma or pulmonary fibrosis treatment medicines
CN110790686B (en) * 2019-10-08 2022-03-08 南开大学 Compound and application thereof in preparing anti-inflammatory and acute lung injury, chronic obstructive lung disease, asthma or pulmonary fibrosis treatment medicines
CN113943262A (en) * 2021-11-17 2022-01-18 烟台海川化学制品有限公司 Preparation method of 2-aminobenzoxazole compound

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