WO2004062558A3 - Process for enzymatic synthesis of βετα-lactam antibiotics - Google Patents
Process for enzymatic synthesis of βετα-lactam antibiotics Download PDFInfo
- Publication number
- WO2004062558A3 WO2004062558A3 PCT/EP2004/000182 EP2004000182W WO2004062558A3 WO 2004062558 A3 WO2004062558 A3 WO 2004062558A3 EP 2004000182 W EP2004000182 W EP 2004000182W WO 2004062558 A3 WO2004062558 A3 WO 2004062558A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- lactam antibiotics
- βετα
- enzymatic synthesis
- lactam
- reaction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
- C12P37/04—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by acylation of the substituent in the 6 position
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
- C12P35/04—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by acylation of the substituent in the 7 position
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Cephalosporin Compounds (AREA)
Abstract
The present invention describes a process for the preparation of β-lactam antibiotics, cephalexin and ampicillin, wherein the β-lactam nucleus is acylated with an acylating agent, D-phenylglycine, by means of a reaction biocatalyzed by a catalytic enzyme. The acylation reaction is carried out in solid phase in solvent free systems (water or organic solvents) for the dispersion of reagents and at controlled temperatures.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI20030042 ITMI20030042A1 (en) | 2003-01-14 | 2003-01-14 | ENZYMATIC SYNTHESIS PROCESS OF B LACTAMIC ANTIBIOTICS. |
ITMI2003A000042 | 2003-01-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2004062558A2 WO2004062558A2 (en) | 2004-07-29 |
WO2004062558A3 true WO2004062558A3 (en) | 2005-06-09 |
Family
ID=32697277
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2004/000182 WO2004062558A2 (en) | 2003-01-14 | 2004-01-14 | Process for enzymatic synthesis of βετα-lactam antibiotics |
Country Status (2)
Country | Link |
---|---|
IT (1) | ITMI20030042A1 (en) |
WO (1) | WO2004062558A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102851332A (en) * | 2012-09-07 | 2013-01-02 | 石药集团中诺药业(石家庄)有限公司 | Recovery method for D(-)phenylglycine in ampicillin mother liquid by using enzyme method |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011080739A2 (en) | 2009-12-29 | 2011-07-07 | Yeda Research And Development Co. Ltd. | Synergistic antibiotic combinations and derivatives |
CN106187795A (en) * | 2016-07-05 | 2016-12-07 | 南京红杉生物科技有限公司 | A kind of synthetic method of D Phenylglycine methyl ester |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0730035A1 (en) * | 1995-02-28 | 1996-09-04 | ACS DOBFAR S.p.A. | Improved enzymatic process for producing penicillins and cephalosporins |
WO1997004086A1 (en) * | 1995-07-18 | 1997-02-06 | Gist-Brocades B.V. | An improved immobilized penicillin g acylase |
WO1998020120A1 (en) * | 1996-11-05 | 1998-05-14 | Bristol-Myers Squibb Company | Mutant penicillin g acylases |
WO1999001566A1 (en) * | 1997-07-03 | 1999-01-14 | Dsm N.V. | PREPARATION OF ENZYME WITH REDUCED β-LACTAMASE ACTIVITY |
US6218138B1 (en) * | 1998-05-26 | 2001-04-17 | Unifar Kimya Sanayi Ve Ticaret A.S. | Synthesis of beta-lactam antibiotics with immobilized penicillin amidase |
-
2003
- 2003-01-14 IT ITMI20030042 patent/ITMI20030042A1/en unknown
-
2004
- 2004-01-14 WO PCT/EP2004/000182 patent/WO2004062558A2/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0730035A1 (en) * | 1995-02-28 | 1996-09-04 | ACS DOBFAR S.p.A. | Improved enzymatic process for producing penicillins and cephalosporins |
WO1997004086A1 (en) * | 1995-07-18 | 1997-02-06 | Gist-Brocades B.V. | An improved immobilized penicillin g acylase |
WO1998020120A1 (en) * | 1996-11-05 | 1998-05-14 | Bristol-Myers Squibb Company | Mutant penicillin g acylases |
WO1999001566A1 (en) * | 1997-07-03 | 1999-01-14 | Dsm N.V. | PREPARATION OF ENZYME WITH REDUCED β-LACTAMASE ACTIVITY |
US6218138B1 (en) * | 1998-05-26 | 2001-04-17 | Unifar Kimya Sanayi Ve Ticaret A.S. | Synthesis of beta-lactam antibiotics with immobilized penicillin amidase |
Non-Patent Citations (3)
Title |
---|
ARROYO M ET AL: "Biotechnological applications of penicillin acylases: State-of-the-art.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 60, no. 5, January 2003 (2003-01-01), pages 507 - 514, XP002280220, ISSN: 0175-7598 * |
BELL GEORGE ET AL: "Biocatalyst behaviour in low-water systems", TRENDS IN BIOTECHNOLOGY, vol. 13, no. 11, 1995, pages 468 - 473, XP002280222, ISSN: 0167-7799 * |
FERNANDEZ-LAFUENTE R ET AL: "The presence of methanol exerts a strong and complex modulation of the synthesis of different antibiotics by immobilized penicillin G acylase", ENZYME AND MICROBIAL TECHNOLOGY, vol. 23, no. 5, October 1998 (1998-10-01), pages 305 - 310, XP002280221, ISSN: 0141-0229 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102851332A (en) * | 2012-09-07 | 2013-01-02 | 石药集团中诺药业(石家庄)有限公司 | Recovery method for D(-)phenylglycine in ampicillin mother liquid by using enzyme method |
Also Published As
Publication number | Publication date |
---|---|
ITMI20030042A1 (en) | 2004-07-15 |
WO2004062558A2 (en) | 2004-07-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2004094635A3 (en) | Compositions and methods for using a solid support to purify rna | |
WO2002093131A3 (en) | Methods for isolating and labeling sample molecules | |
WO2004108281A3 (en) | In-situ zsm-5 synthesis | |
AU2002246125A1 (en) | Process for preparing stabilized reaction mixtures which are partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures | |
WO2005010209A3 (en) | Method for the reverse transcription and/or amplification of nucleic acids | |
WO2005044836A3 (en) | Macromolecular nucleotide compounds and methods for using the same | |
EP2514828A3 (en) | Methods for the production of ansamitocins | |
WO2007125429A3 (en) | Method for the synthesis of triazole- containing oligonucleotide derivatives | |
WO2006013072A3 (en) | Method for isolation of transcription termination sequences | |
WO2003012124A3 (en) | Methods for immobilizing molecules to a solid phase and uses thereof | |
WO2002099080A3 (en) | Methods for low background cloning of dna using long oligonucleotides | |
WO2004062558A3 (en) | Process for enzymatic synthesis of βετα-lactam antibiotics | |
AU2003226885A1 (en) | Novel method for production of dna biochips and applications thereof | |
EP2292766A3 (en) | Polypeptides having DNA polymerase activity | |
EP2173892B8 (en) | Process for the preparation of immobilised recombinant Penicillin Acylase biocatalyst from Achromobacter sp CCM 4824 expressed in E. coli BL21 CCM 7394 and its use for the synthesis of beta-lactam antiobiotics | |
Wei et al. | Effects of ethylene glycol on the synthesis of ampicillin using immobilized penicillin G acylase | |
WO2005017202A3 (en) | Method and kit for identifying antibiotic-resistant microorganisms | |
WO2001032935A3 (en) | Molecular microarrays and methods for production and use thereof | |
AU2003210277A1 (en) | Environment friendly reagents and process for trifluoromethylsulfinylation of organic compounds | |
Aguirre et al. | Effect of cosolvent and pH on the kinetically controlled synthesis of cephalexin with immobilised penicillin acylase | |
WO2004027056A3 (en) | Methods of use for thermostable rna ligases | |
WO2001059126A3 (en) | Gene cluster encoding a nystatin polyketide synthase and its manipulation and utility | |
WO2002102504A3 (en) | The production of molecular arrays | |
WO2005122671A3 (en) | An improved process for the preparation of oxcarbazepine and related intermediates | |
AU2003266255A1 (en) | Method for amplification of nucleic acids of low complexity |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): BW GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
DPEN | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed from 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
122 | Ep: pct application non-entry in european phase |