WO2004056962A8 - Constructs homogenously processed preparations of beta site app-cleaving enzyme - Google Patents

Constructs homogenously processed preparations of beta site app-cleaving enzyme

Info

Publication number
WO2004056962A8
WO2004056962A8 PCT/US2003/038314 US0338314W WO2004056962A8 WO 2004056962 A8 WO2004056962 A8 WO 2004056962A8 US 0338314 W US0338314 W US 0338314W WO 2004056962 A8 WO2004056962 A8 WO 2004056962A8
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
constructs
engineered
cleaving enzyme
site app
Prior art date
Application number
PCT/US2003/038314
Other languages
French (fr)
Other versions
WO2004056962A3 (en
WO2004056962A2 (en
Inventor
Marcus Ballinger
Original Assignee
Sunesis Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sunesis Pharmaceuticals Inc filed Critical Sunesis Pharmaceuticals Inc
Priority to AU2003302738A priority Critical patent/AU2003302738A1/en
Publication of WO2004056962A2 publication Critical patent/WO2004056962A2/en
Publication of WO2004056962A8 publication Critical patent/WO2004056962A8/en
Publication of WO2004056962A3 publication Critical patent/WO2004056962A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention is directed to engineered polypeptides having BACE activity. In certain embodiments, the polypeptides also comprise an engineered cleavage site. Also provided are polypeptides comprising a prodomain, an engineered cleavage site, and a protease domain; the polypeptides are properly folded and are cleaved at the engineered cleavage site in vitro, producing homogeneous preparations of purified protease having BACE activity. The invention further pertains to nucleic acids, expression vectors, and host cells comprising the expression vectors for making the engineered polypeptides.
PCT/US2003/038314 2002-12-04 2003-12-02 Constructs homogenously processed preparations of beta site app-cleaving enzyme WO2004056962A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003302738A AU2003302738A1 (en) 2002-12-04 2003-12-02 Constructs homogenously processed preparations of beta site app-cleaving enzyme

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43098402P 2002-12-04 2002-12-04
US60/430,984 2002-12-04

Publications (3)

Publication Number Publication Date
WO2004056962A2 WO2004056962A2 (en) 2004-07-08
WO2004056962A8 true WO2004056962A8 (en) 2005-03-31
WO2004056962A3 WO2004056962A3 (en) 2006-02-16

Family

ID=32681941

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/038314 WO2004056962A2 (en) 2002-12-04 2003-12-02 Constructs homogenously processed preparations of beta site app-cleaving enzyme

Country Status (2)

Country Link
AU (1) AU2003302738A1 (en)
WO (1) WO2004056962A2 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6699671B1 (en) * 1998-09-24 2004-03-02 Pharmacia & Upjohn Company Alzheimer's disease secretase, APP substrates therefor, and uses therefor

Also Published As

Publication number Publication date
WO2004056962A3 (en) 2006-02-16
WO2004056962A2 (en) 2004-07-08
AU2003302738A8 (en) 2004-07-14
AU2003302738A1 (en) 2004-07-14

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