WO2004053160A3 - Method to analyze polymeric nucleic acid sequence variations - Google Patents

Method to analyze polymeric nucleic acid sequence variations Download PDF

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Publication number
WO2004053160A3
WO2004053160A3 PCT/GB2003/005341 GB0305341W WO2004053160A3 WO 2004053160 A3 WO2004053160 A3 WO 2004053160A3 GB 0305341 W GB0305341 W GB 0305341W WO 2004053160 A3 WO2004053160 A3 WO 2004053160A3
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WO
WIPO (PCT)
Prior art keywords
provides
exon
rna
save
self
Prior art date
Application number
PCT/GB2003/005341
Other languages
French (fr)
Other versions
WO2004053160A2 (en
Inventor
Maria Concepcion Jimenez
Irene Gascon Escobar
Susana Coca Gallego
Jaun Carlos Rodrig Cimadevilla
Original Assignee
Genomica Sau
Maria Concepcion Jimenez
Irene Gascon Escobar
Susana Coca Gallego
Jaun Carlos Rodrig Cimadevilla
Ruffles Graham Keith
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomica Sau, Maria Concepcion Jimenez, Irene Gascon Escobar, Susana Coca Gallego, Jaun Carlos Rodrig Cimadevilla, Ruffles Graham Keith filed Critical Genomica Sau
Priority to AU2003298437A priority Critical patent/AU2003298437A1/en
Publication of WO2004053160A2 publication Critical patent/WO2004053160A2/en
Publication of WO2004053160A3 publication Critical patent/WO2004053160A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to methods to assess exon-intron boundaries, transcription start points as well as real experimental polyadenylation sites as utilized by a given cell, tissue or organism in a given time or situation (physiological or pathological). Self-nornmalisation (SN) provides a method to change the partial representation of transcripts in a self-normalising manner. Five prime exon rescue (FER) provides a method to analyse 5´ exon forks in sense-RNA/anti-RNA hybrids. String analysis of variant exon (SAVE) provides a method to link together complete RNA fragments resulting from RNase VI digestion. By using RNA linkers, SAVE also provides a method to generate strings of RNA fragments, to amplify them, to clone them, to sequence them and to purify them for further use as well.
PCT/GB2003/005341 2002-12-06 2003-12-08 Method to analyze polymeric nucleic acid sequence variations WO2004053160A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003298437A AU2003298437A1 (en) 2002-12-06 2003-12-08 Method to analyze polymeric nucleic acid sequence variations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0228557.5 2002-12-06
GBGB0228557.5A GB0228557D0 (en) 2002-12-06 2002-12-06 Method to analyze polymeric nucleic acid sequence variations

Publications (2)

Publication Number Publication Date
WO2004053160A2 WO2004053160A2 (en) 2004-06-24
WO2004053160A3 true WO2004053160A3 (en) 2004-08-05

Family

ID=9949249

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/005341 WO2004053160A2 (en) 2002-12-06 2003-12-08 Method to analyze polymeric nucleic acid sequence variations

Country Status (3)

Country Link
AU (1) AU2003298437A1 (en)
GB (1) GB0228557D0 (en)
WO (1) WO2004053160A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203984B1 (en) * 1998-07-02 2001-03-20 Pacron, Inc. Proportional amplification of mRNA from a linear template in vitro
US6251590B1 (en) * 1998-03-11 2001-06-26 Exonhit Therapeutics S.A. Differential Qualitative screening

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251590B1 (en) * 1998-03-11 2001-06-26 Exonhit Therapeutics S.A. Differential Qualitative screening
US6203984B1 (en) * 1998-07-02 2001-03-20 Pacron, Inc. Proportional amplification of mRNA from a linear template in vitro

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JOHNSTON J A ET AL: "A new quantitative solution hybridisation-RNase protection assay for APP and APLP2 mRNA.", BRAIN RESEARCH. MOLECULAR BRAIN RESEARCH. 31 DEC 1996, vol. 43, no. 1-2, 31 December 1996 (1996-12-31), pages 77 - 84, XP002282959, ISSN: 0169-328X *
YAMAMOTO M ET AL: "Use of serial analysis of gene expression (SAGE) technology", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 250, no. 1-2, 1 April 2001 (2001-04-01), pages 45 - 66, XP004230694, ISSN: 0022-1759 *

Also Published As

Publication number Publication date
AU2003298437A1 (en) 2004-06-30
AU2003298437A8 (en) 2004-06-30
GB0228557D0 (en) 2003-01-15
WO2004053160A2 (en) 2004-06-24

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