WO2004050880A3 - Insecticidal agent - Google Patents

Insecticidal agent Download PDF

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Publication number
WO2004050880A3
WO2004050880A3 PCT/GB2003/005183 GB0305183W WO2004050880A3 WO 2004050880 A3 WO2004050880 A3 WO 2004050880A3 GB 0305183 W GB0305183 W GB 0305183W WO 2004050880 A3 WO2004050880 A3 WO 2004050880A3
Authority
WO
WIPO (PCT)
Prior art keywords
resistant
toxin
insecticidal agent
recombinant
lepidopterans
Prior art date
Application number
PCT/GB2003/005183
Other languages
French (fr)
Other versions
WO2004050880A2 (en
WO2004050880B1 (en
Inventor
Neil Crickmore
Original Assignee
Univ Sussex
Neil Crickmore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Sussex, Neil Crickmore filed Critical Univ Sussex
Priority to AU2003285548A priority Critical patent/AU2003285548A1/en
Publication of WO2004050880A2 publication Critical patent/WO2004050880A2/en
Publication of WO2004050880A3 publication Critical patent/WO2004050880A3/en
Publication of WO2004050880B1 publication Critical patent/WO2004050880B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Recombinant Bacillus thuringiensis Cry toxins modified such that the N-terminal region of the protoxin is substantially resistant to protease attack show increased insecticidal activity in lepidopterans resistant to the wild type toxin. The recombinant toxin can be expressed in planta or in bacteria, for instance.
PCT/GB2003/005183 2002-11-29 2003-11-28 Insecticidal agent WO2004050880A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003285548A AU2003285548A1 (en) 2002-11-29 2003-11-28 Insecticidal agent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0227919.8 2002-11-29
GBGB0227919.8A GB0227919D0 (en) 2002-11-29 2002-11-29 Insecticidal agent

Publications (3)

Publication Number Publication Date
WO2004050880A2 WO2004050880A2 (en) 2004-06-17
WO2004050880A3 true WO2004050880A3 (en) 2004-07-29
WO2004050880B1 WO2004050880B1 (en) 2004-09-16

Family

ID=9948806

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/005183 WO2004050880A2 (en) 2002-11-29 2003-11-28 Insecticidal agent

Country Status (3)

Country Link
AU (1) AU2003285548A1 (en)
GB (1) GB0227919D0 (en)
WO (1) WO2004050880A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3192494A1 (en) * 2014-12-30 2016-07-07 Corteva Agriscience Llc Modified cry1ca toxins useful for control of insect pests

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014562A1 (en) * 1999-08-19 2001-03-01 Syngenta Participations Ag Hybrid insecticidal toxins and nucleic acid sequences coding therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014562A1 (en) * 1999-08-19 2001-03-01 Syngenta Participations Ag Hybrid insecticidal toxins and nucleic acid sequences coding therefor

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRAVO ALEJANDRA ET AL: "N-terminal activation is an essential early step in the mechanism of action of the Bacillus thuringiensis Cry1Ac insecticidal toxin", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 27, 5 July 2002 (2002-07-05), pages 23985 - 23987, XP002282587, ISSN: 0021-9258 *
MARTENS JOHN W M ET AL: "Mapping and characterization of the entomocidal domain of the Bacillus thuringiensis CryIA(b) protoxin", MOLECULAR AND GENERAL GENETICS, vol. 247, no. 4, 1995, pages 482 - 487, XP001189552, ISSN: 0026-8925 *
MIRANDA RAUL ET AL: "Processing of Cry1Ab delta-endotoxin from Bacillus thuringiensis by Manduca sexta and Spodoptera frugiperda midgut proteases: Role in protoxin activation and toxin inactivation", INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 31, no. 12, 1 November 2001 (2001-11-01), pages 1155 - 1163, XP002282588, ISSN: 0965-1748 *
STRIZHOV N ET AL: "MAPPING OF THE ENTOMOCIDAL FRAGMENT OF SPODOPTERA-SPECIFIC BACILLUS THURINGIENSIS TOXIN CRYIC", MOLECULAR AND GENERAL GENETICS, SPRINGER VERLAG, BERLIN, DE, vol. 253, no. 1/2, 27 November 1996 (1996-11-27), pages 11 - 19, XP002064601, ISSN: 0026-8925 *

Also Published As

Publication number Publication date
WO2004050880A2 (en) 2004-06-17
AU2003285548A8 (en) 2004-06-23
WO2004050880B1 (en) 2004-09-16
AU2003285548A1 (en) 2004-06-23
GB0227919D0 (en) 2003-01-08

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