WO2004041840A3 - Chromogenes a liaison peptidique servant a determiner des activites enzymatiques - Google Patents

Chromogenes a liaison peptidique servant a determiner des activites enzymatiques Download PDF

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Publication number
WO2004041840A3
WO2004041840A3 PCT/EP2003/012463 EP0312463W WO2004041840A3 WO 2004041840 A3 WO2004041840 A3 WO 2004041840A3 EP 0312463 W EP0312463 W EP 0312463W WO 2004041840 A3 WO2004041840 A3 WO 2004041840A3
Authority
WO
WIPO (PCT)
Prior art keywords
peptide
atoms
crs
enzymatic activities
amino
Prior art date
Application number
PCT/EP2003/012463
Other languages
German (de)
English (en)
Other versions
WO2004041840A2 (fr
Inventor
Stefan Spichiger
Ursula Spichiger-Keller
Michael Linnhoff
Gleb Zhylyak
Original Assignee
C Cit Ag
Fluka Gmbh
Stefan Spichiger
Ursula Spichiger-Keller
Michael Linnhoff
Gleb Zhylyak
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by C Cit Ag, Fluka Gmbh, Stefan Spichiger, Ursula Spichiger-Keller, Michael Linnhoff, Gleb Zhylyak filed Critical C Cit Ag
Priority to AU2003286158A priority Critical patent/AU2003286158A1/en
Publication of WO2004041840A2 publication Critical patent/WO2004041840A2/fr
Publication of WO2004041840A3 publication Critical patent/WO2004041840A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/081—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/50—Lipopolysaccharides; LPS
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un composé chimique de formule générale (I) : X-A-B-C-L-CRS. Dans ladite formule : X représente hydrogène ou un groupe de protection de groupes amino terminaux courant en chimie des peptides ; A et B représentent un α-, β-, ou η-amino-acide comportant 2 à 15 atomes de C, jusqu'à 4 atomes de N, jusqu'à 2 atomes de S et jusqu'à 6 atomes d'O, A et B pouvant être identiques ou différents et B représentant éventuellement un dipeptide formé par lesdits amino-acides ; C représente arginine, lysine, tyrosine, phénylalanine ou tryptophane ou leurs homologues respectifs ; L représente un lieur pouvant former une liaison peptidique avec C ; CRS représente un système cyclique carboné conjugué relié au radical -L-C-B-A-X par l'intermédiaire d'un atome d'azote. Selon l'invention, ledit composé de formule générale (I) et CRS présentent un maximum d'absorption à une longueur d'onde supérieure à 550 nm.
PCT/EP2003/012463 2002-11-07 2003-11-07 Chromogenes a liaison peptidique servant a determiner des activites enzymatiques WO2004041840A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003286158A AU2003286158A1 (en) 2002-11-07 2003-11-07 Peptide-bound chromogens used for determining enzymatic activities

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10251894.7 2002-11-07
DE10251894A DE10251894A1 (de) 2002-11-07 2002-11-07 Peptidgebundene Chromogene zur Bestimmung von Enzymaktivitäten

Publications (2)

Publication Number Publication Date
WO2004041840A2 WO2004041840A2 (fr) 2004-05-21
WO2004041840A3 true WO2004041840A3 (fr) 2004-07-01

Family

ID=32115349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/012463 WO2004041840A2 (fr) 2002-11-07 2003-11-07 Chromogenes a liaison peptidique servant a determiner des activites enzymatiques

Country Status (3)

Country Link
AU (1) AU2003286158A1 (fr)
DE (1) DE10251894A1 (fr)
WO (1) WO2004041840A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7671139B1 (en) 2004-06-18 2010-03-02 Bridgestone Corporation Functionalized polymers and tires made therefrom
FR2875242B1 (fr) * 2004-09-10 2006-11-17 Biomerieux Sa Nouveaux substrats enzymatiques derives de phenoxazinone et leur utilisation comme revelateur dans la detection de microorganismes a activite peptidase
EP1674580A1 (fr) * 2004-12-23 2006-06-28 F. Hoffmann-La Roche Ag Procédé d'identification d'activateurs et/ou d'inhibiteurs d'activité enzymatique
EP2465941A1 (fr) * 2010-12-20 2012-06-20 Siemens Healthcare Diagnostics Products GmbH Procédé de détermination simultanée de plusieurs protéases de coagulation

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0228666A2 (fr) * 1986-01-06 1987-07-15 Orion Corporation Ltd Substrats de type peptide et procédé pour la détermination quantitative d'endotoxine
US5097014A (en) * 1986-08-28 1992-03-17 Behringwerke Aktiengesellschaft Chromogenic tripeptides
US5175089A (en) * 1988-07-15 1992-12-29 The Trustees Of Columbia University In The City Of New York Method for monitoring periodontal disease by monitoring endotoxins and inflammatory agents
US5334703A (en) * 1989-12-12 1994-08-02 Chromonogenix Ab Chromogenic substrate

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0228666A2 (fr) * 1986-01-06 1987-07-15 Orion Corporation Ltd Substrats de type peptide et procédé pour la détermination quantitative d'endotoxine
US5097014A (en) * 1986-08-28 1992-03-17 Behringwerke Aktiengesellschaft Chromogenic tripeptides
US5175089A (en) * 1988-07-15 1992-12-29 The Trustees Of Columbia University In The City Of New York Method for monitoring periodontal disease by monitoring endotoxins and inflammatory agents
US5334703A (en) * 1989-12-12 1994-08-02 Chromonogenix Ab Chromogenic substrate

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BERGER D ET AL: "QUANTITATIVE ENDOTOXIN DETERMINATION IN BLOOD CHROMOGENIC MODIFICATION OF THE LIMULUS AMOEBOCYTE LYSATE TEST", EUROPEAN SURGICAL RESEARCH, vol. 20, no. 2, 1988, pages 128 - 136, XP009030587, ISSN: 0014-312X *
SEILER K ET AL: "CHARACTERIZATION OF SODIUM-SELECTIVE OPTODE MEMBRANES BASED ON NEUTRAL LONOPHORES AND ASSAY OF SODIUM IN PLASMA", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY. WINSTON, US, vol. 37, no. 8, 1 August 1991 (1991-08-01), pages 1350 - 1355, XP000226105, ISSN: 0009-9147 *

Also Published As

Publication number Publication date
WO2004041840A2 (fr) 2004-05-21
AU2003286158A8 (en) 2004-06-07
DE10251894A1 (de) 2004-05-19
AU2003286158A1 (en) 2004-06-07

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