WO2004039987A3 - Methods for increasing the efficiency of homologous recombination in cells of vascular plants - Google Patents

Methods for increasing the efficiency of homologous recombination in cells of vascular plants Download PDF

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Publication number
WO2004039987A3
WO2004039987A3 PCT/EP2003/011726 EP0311726W WO2004039987A3 WO 2004039987 A3 WO2004039987 A3 WO 2004039987A3 EP 0311726 W EP0311726 W EP 0311726W WO 2004039987 A3 WO2004039987 A3 WO 2004039987A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
increasing
efficiency
methods
homologous recombination
Prior art date
Application number
PCT/EP2003/011726
Other languages
German (de)
French (fr)
Other versions
WO2004039987A2 (en
Inventor
Ralf Badur
Erwin Heberle-Bors
Original Assignee
Basf Plant Science Gmbh
Ralf Badur
Erwin Heberle-Bors
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh, Ralf Badur, Erwin Heberle-Bors filed Critical Basf Plant Science Gmbh
Priority to AU2003278126A priority Critical patent/AU2003278126A1/en
Publication of WO2004039987A2 publication Critical patent/WO2004039987A2/en
Publication of WO2004039987A3 publication Critical patent/WO2004039987A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention relates to methods for increasing the efficiency of homologous recombination in cells of vascular plants by inducing a G2/M arrest in the cell cycle thereof.
PCT/EP2003/011726 2002-10-29 2003-10-23 Methods for increasing the efficiency of homologous recombination in cells of vascular plants WO2004039987A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003278126A AU2003278126A1 (en) 2002-10-29 2003-10-23 Methods for increasing the efficiency of homologous recombination in cells of vascular plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10250529 2002-10-29
DE10250529.2 2002-10-29

Publications (2)

Publication Number Publication Date
WO2004039987A2 WO2004039987A2 (en) 2004-05-13
WO2004039987A3 true WO2004039987A3 (en) 2004-08-05

Family

ID=32185286

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/011726 WO2004039987A2 (en) 2002-10-29 2003-10-23 Methods for increasing the efficiency of homologous recombination in cells of vascular plants

Country Status (2)

Country Link
AU (1) AU2003278126A1 (en)
WO (1) WO2004039987A2 (en)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BINAROVA PAVLA ET AL: "Treatment of Vicia faba root tip cells with specific inhibitors to cyclin-dependent kinases leads to abnormal spindle formation", PLANT JOURNAL, vol. 16, no. 6, December 1998 (1998-12-01), pages 697 - 707, XP002267381, ISSN: 0960-7412 *
MENGISTE T ET AL: "PROSPECTS FOR THE PRECISE ENGINEERING OF PLANT GENOMES BY HOMOLOGOUS RECOMBINATION", BIOLOGICAL CHEMISTRY, vol. 380, no. 7/8, 1999, pages 749 - 758, XP000885763, ISSN: 1431-6730 *
SCHWEEN G ET AL: "Unique tissue-specific cell cycle in Physcomitrella.", PLANT BIOLOGY (STUTTGART), vol. 5, no. 1, January 2003 (2003-01-01), pages 50 - 58, XP009024242, ISSN: 1435-8603 *
STREPP ET AL: "Plant nuclar gene kockout reveals a role in plastid division for the homolog of the bacterial cell division protein FtsZ, an ancestral tubulin", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 95, 1 April 1998 (1998-04-01), pages 4368 - 4373, XP002083808, ISSN: 0027-8424 *
TAKATA MINORU ET AL: "Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 17, no. 18, 15 September 1998 (1998-09-15), pages 5497 - 5508, XP002267382, ISSN: 0261-4189 *

Also Published As

Publication number Publication date
WO2004039987A2 (en) 2004-05-13
AU2003278126A1 (en) 2004-05-25

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