WO2004035764A3 - PROTEIN SYNTHESIS WITH TANDEMLY ACTIVATED tRNAs - Google Patents

PROTEIN SYNTHESIS WITH TANDEMLY ACTIVATED tRNAs Download PDF

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Publication number
WO2004035764A3
WO2004035764A3 PCT/US2003/033459 US0333459W WO2004035764A3 WO 2004035764 A3 WO2004035764 A3 WO 2004035764A3 US 0333459 W US0333459 W US 0333459W WO 2004035764 A3 WO2004035764 A3 WO 2004035764A3
Authority
WO
WIPO (PCT)
Prior art keywords
trnas
protein synthesis
tandemly
activated
protein
Prior art date
Application number
PCT/US2003/033459
Other languages
French (fr)
Other versions
WO2004035764A2 (en
Inventor
Sidney M Hecht
Bixum Wang
Michiel Lodder
Original Assignee
Univ Virginia
Sidney M Hecht
Bixum Wang
Michiel Lodder
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Virginia, Sidney M Hecht, Bixum Wang, Michiel Lodder filed Critical Univ Virginia
Priority to AU2003301314A priority Critical patent/AU2003301314A1/en
Publication of WO2004035764A2 publication Critical patent/WO2004035764A2/en
Publication of WO2004035764A3 publication Critical patent/WO2004035764A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to 2', 3'-bis-O-aminoacylated tRNAs and their use to enhance the yields of in vitro protein synthesis. Advantageously, the bisaminoacyl-tRNAs are more stable than corresponding mono-aminoacyl-tRNAs against deacylation and are shown herein to function in protein biosynthesis, contributing up to two amino acids to the derived protein.
PCT/US2003/033459 2002-10-17 2003-10-16 PROTEIN SYNTHESIS WITH TANDEMLY ACTIVATED tRNAs WO2004035764A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003301314A AU2003301314A1 (en) 2002-10-17 2003-10-16 PROTEIN SYNTHESIS WITH TANDEMLY ACTIVATED tRNAs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41931102P 2002-10-17 2002-10-17
US60/419,311 2002-10-17

Publications (2)

Publication Number Publication Date
WO2004035764A2 WO2004035764A2 (en) 2004-04-29
WO2004035764A3 true WO2004035764A3 (en) 2004-10-21

Family

ID=32108063

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/033459 WO2004035764A2 (en) 2002-10-17 2003-10-16 PROTEIN SYNTHESIS WITH TANDEMLY ACTIVATED tRNAs

Country Status (2)

Country Link
AU (1) AU2003301314A1 (en)
WO (1) WO2004035764A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1674573A1 (en) * 2004-12-23 2006-06-28 Ecole Polytechnique Federale De Lausanne RNA-sequence or nucleoside/nucleotide analogues and method for producing the analogues

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KARGINOV V.A.: "Probing The Role Of An Active Site Aspartic Acid In Dihydrofolate Reductase", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 119, 1997, pages 8166 - 8176, XP000891978 *
LODDER M. ET AL: "Misacylated Transfer RNAs Having a Chemically Removable Protecting Group", THE JOURNAL OF ORGANIC CHEMISTRY, vol. 63, 1998, pages 794 - 803, XP002980426 *

Also Published As

Publication number Publication date
AU2003301314A1 (en) 2004-05-04
AU2003301314A8 (en) 2004-05-04
WO2004035764A2 (en) 2004-04-29

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