WO2004035621A3 - Rad51-brc repeat crystals - Google Patents

Rad51-brc repeat crystals Download PDF

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Publication number
WO2004035621A3
WO2004035621A3 PCT/GB2003/004485 GB0304485W WO2004035621A3 WO 2004035621 A3 WO2004035621 A3 WO 2004035621A3 GB 0304485 W GB0304485 W GB 0304485W WO 2004035621 A3 WO2004035621 A3 WO 2004035621A3
Authority
WO
WIPO (PCT)
Prior art keywords
rad51
crystals
brc repeat
brc
repeat sequence
Prior art date
Application number
PCT/GB2003/004485
Other languages
French (fr)
Other versions
WO2004035621A2 (en
Inventor
Ashok Venkitaraman
Luca Pellegrini
Tom Blundell
David Yu
Thomas Lo
Debbie Bates
Mahmud Shivji
Shubha Anand
Miyoung Lee
Original Assignee
Univ Cambridge Tech
Ashok Venkitaraman
Luca Pellegrini
Tom Blundell
David Yu
Thomas Lo
Debbie Bates
Mahmud Shivji
Shubha Anand
Miyoung Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Cambridge Tech, Ashok Venkitaraman, Luca Pellegrini, Tom Blundell, David Yu, Thomas Lo, Debbie Bates, Mahmud Shivji, Shubha Anand, Miyoung Lee filed Critical Univ Cambridge Tech
Priority to US10/531,242 priority Critical patent/US20060234293A1/en
Priority to AU2003271967A priority patent/AU2003271967A1/en
Priority to EP03753806A priority patent/EP1551871A2/en
Publication of WO2004035621A2 publication Critical patent/WO2004035621A2/en
Publication of WO2004035621A3 publication Critical patent/WO2004035621A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)

Abstract

The structure of a RAD51-BRC repeat sequence complex structure is provided. The structure can be used in modelling the interaction of molecular structures such as potential pharmaceutical compounds. Mutant RAD51 and BRCA2 polypeptides and RAD51-BRC repeat sequence chimaera proteins and are also provided. The mutants may be used in assays for finding compounds which interact with or form part of a RAD51 pathway, and the chimaeras can be used to form crystals which may be analysed by X-ray crystallography.
PCT/GB2003/004485 2002-10-14 2003-10-14 Rad51-brc repeat crystals WO2004035621A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/531,242 US20060234293A1 (en) 2002-10-14 2003-10-14 Polypeptide methods and means
AU2003271967A AU2003271967A1 (en) 2002-10-14 2003-10-14 Rad51-brc repeat crystals
EP03753806A EP1551871A2 (en) 2002-10-14 2003-10-14 Rad51-brc repeat crystals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0223860.8A GB0223860D0 (en) 2002-10-14 2002-10-14 Polypeptide methods and means
GB0223860.8 2002-10-14

Publications (2)

Publication Number Publication Date
WO2004035621A2 WO2004035621A2 (en) 2004-04-29
WO2004035621A3 true WO2004035621A3 (en) 2004-08-26

Family

ID=9945880

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/004485 WO2004035621A2 (en) 2002-10-14 2003-10-14 Rad51-brc repeat crystals

Country Status (5)

Country Link
US (1) US20060234293A1 (en)
EP (2) EP1551871A2 (en)
AU (1) AU2003271967A1 (en)
GB (1) GB0223860D0 (en)
WO (1) WO2004035621A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11208444B2 (en) * 2015-03-05 2021-12-28 Idea Seed, LLC. BRCA2-mediated purification of recombinase protein
WO2016141390A1 (en) 2015-03-05 2016-09-09 Michael Longo Recombinase purification

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998020030A2 (en) * 1996-11-05 1998-05-14 Lexicon Genetics Incorporated DISRUPTION OF THE MAMMALIAN Rad51 PROTEIN AND DISRUPTION OF PROTEINS THAT ASSOCIATE WITH MAMMALIAN Rad51 FOR HINDERING CELL PROLIFERATION

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998020030A2 (en) * 1996-11-05 1998-05-14 Lexicon Genetics Incorporated DISRUPTION OF THE MAMMALIAN Rad51 PROTEIN AND DISRUPTION OF PROTEINS THAT ASSOCIATE WITH MAMMALIAN Rad51 FOR HINDERING CELL PROLIFERATION

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
AIHARA H ET AL: "The N-terminal domain of the human Rad51 protein binds DNA: structure and a DNA binding surface as revealed by NMR", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 290, no. 2, 9 July 1999 (1999-07-09), pages 495 - 504, XP004462010, ISSN: 0022-2836 *
KREJCI LUMIR ET AL: "Molecular dissection of interactions between Rad51 and members of the recombination-repair group", MOLECULAR AND CELLULAR BIOLOGY, vol. 21, no. 3, February 2001 (2001-02-01), pages 966 - 976, XP002269102, ISSN: 0270-7306 *
PELLEGRINI L ET AL: "INSIGHTS INTO DNA RECOMBINATION FROM THE STRUCTURE OF A RAD51-BRCA2 COMPLEX", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 420, no. 6913, 21 November 2002 (2002-11-21), pages 287 - 293, XP001184749, ISSN: 0028-0836 *
STORY R M ET AL: "THE STRUCTURE OF THE E. COLI RECA PROTEIN MONOMER AND POLYMER", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 355, no. 6358, 23 January 1992 (1992-01-23), pages 318 - 325, XP008026763, ISSN: 0028-0836 *
VENKITARAMAN ASHOK R: "Cancer susceptibility and the functions of BRCA1 and BRCA2", CELL, vol. 108, no. 2, 25 January 2002 (2002-01-25), pages 171 - 182, XP002269104, ISSN: 0092-8674 *
WONG ALEXANDER K C ET AL: "RAD51 interacts with the evolutionarily conserved BRC motifs in the human breast cancer susceptibility gene brca2", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 51, 19 December 1997 (1997-12-19), pages 31941 - 31944, XP002269099, ISSN: 0021-9258 *
YANG HAIJUAN ET AL: "BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure", SCIENCE (WASHINGTON D C), vol. 297, no. 5588, 13 September 2002 (2002-09-13), pages 1837 - 1848, XP002269103, ISSN: 0036-8075 *
YANG SHIXIN ET AL: "Comparison of bacteriophage T4 UvsX and human Rad51 filaments suggests that RecA-like polymers may have evolved independently", JOURNAL OF MOLECULAR BIOLOGY, vol. 312, no. 5, 5 October 2001 (2001-10-05), pages 999 - 1009, XP002269101, ISSN: 0022-2836 *

Also Published As

Publication number Publication date
GB0223860D0 (en) 2002-11-20
US20060234293A1 (en) 2006-10-19
AU2003271967A8 (en) 2004-05-04
WO2004035621A2 (en) 2004-04-29
EP1650223A2 (en) 2006-04-26
EP1650223A3 (en) 2006-07-26
AU2003271967A1 (en) 2004-05-04
EP1551871A2 (en) 2005-07-13

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