WO2004029301A3 - Method for assaying replication of hbv and testing susceptibility to drugs - Google Patents

Method for assaying replication of hbv and testing susceptibility to drugs Download PDF

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Publication number
WO2004029301A3
WO2004029301A3 PCT/EP2003/012398 EP0312398W WO2004029301A3 WO 2004029301 A3 WO2004029301 A3 WO 2004029301A3 EP 0312398 W EP0312398 W EP 0312398W WO 2004029301 A3 WO2004029301 A3 WO 2004029301A3
Authority
WO
WIPO (PCT)
Prior art keywords
hbv
possibly
pgrna
pharmaceutical product
replication
Prior art date
Application number
PCT/EP2003/012398
Other languages
French (fr)
Other versions
WO2004029301A2 (en
Inventor
David Durantel
Sandra Durantel
Christian Trepo
Fabien Zoulim
Original Assignee
Inst Nat Sante Rech Med
David Durantel
Sandra Durantel
Christian Trepo
Fabien Zoulim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Nat Sante Rech Med, David Durantel, Sandra Durantel, Christian Trepo, Fabien Zoulim filed Critical Inst Nat Sante Rech Med
Priority to EP03785637A priority Critical patent/EP1543168B1/en
Priority to CA002500188A priority patent/CA2500188A1/en
Priority to AT03785637T priority patent/ATE468411T1/en
Priority to US10/528,833 priority patent/US20060240405A1/en
Priority to DE60332641T priority patent/DE60332641D1/en
Priority to AU2003294704A priority patent/AU2003294704A1/en
Publication of WO2004029301A2 publication Critical patent/WO2004029301A2/en
Publication of WO2004029301A3 publication Critical patent/WO2004029301A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method for measuring the replication capacity of HBV, e.g. HBV present in a biological sample, possibly in the presence of a pharmaceutical product, in particular an antiviral agent, the method comprising: (a) possibly extracting nucleic acids contained in the biological sample; (b) PCR amplifying HBV nucleic acids using at least two primer pairs selected so as to obtain at least two different amplified HBV genomic fragments which upon assembly represent a linear continuous DNA sequence transcriptable in pgRNA ; (c) cloning the fragments obtained under (b) into a vector under the control of an heterologous promoter, so producing a vector containing a linear continuous DNA sequence transcriptable in pgRNA under control of said promoter; (d) transfecting or transducing susceptible cells with the vector; (e) culturing the transfected or transduced cells in conditions allowing synthesis of HBV pgRNA from the cloned HBV DNA; (f) possibly treating the cultured cells with the pharmaceutical product, in particular antiviral agent; and (g) determining the replication capacity of the HBV, possibly incidence of the pharmaceutical product, preferably antiviral agent, on viral gene expression and/or viral replication.
PCT/EP2003/012398 2002-09-27 2003-09-26 Method for assaying replication of hbv and testing susceptibility to drugs WO2004029301A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP03785637A EP1543168B1 (en) 2002-09-27 2003-09-26 Method for assaying replication of hbv and testing susceptibility to drugs
CA002500188A CA2500188A1 (en) 2002-09-27 2003-09-26 Method for assaying replication of hbv and testing susceptibility to drugs
AT03785637T ATE468411T1 (en) 2002-09-27 2003-09-26 METHOD FOR DISPLAYING HBV REPLICATION AND TESTING THE SENSITIVITY OF DRUGS
US10/528,833 US20060240405A1 (en) 2002-09-27 2003-09-26 Method for assaying replication of hbv and testing ... drugs
DE60332641T DE60332641D1 (en) 2002-09-27 2003-09-26 METHOD FOR PRESENTING THE REPLICATION OF HBV AND TESTING THE SENSITIVITY OF ACTIVE SUBSTANCES
AU2003294704A AU2003294704A1 (en) 2002-09-27 2003-09-26 Method for assaying replication of hbv and testing susceptibility to drugs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02356188.9 2002-09-27
EP20020356188 EP1403385A1 (en) 2002-09-27 2002-09-27 Method for assaying replication of HBV and testing susceptibility to drugs

Publications (2)

Publication Number Publication Date
WO2004029301A2 WO2004029301A2 (en) 2004-04-08
WO2004029301A3 true WO2004029301A3 (en) 2005-02-24

Family

ID=31970489

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/012398 WO2004029301A2 (en) 2002-09-27 2003-09-26 Method for assaying replication of hbv and testing susceptibility to drugs

Country Status (9)

Country Link
US (1) US20060240405A1 (en)
EP (2) EP1403385A1 (en)
CN (1) CN100562586C (en)
AT (1) ATE468411T1 (en)
AU (1) AU2003294704A1 (en)
CA (1) CA2500188A1 (en)
DE (1) DE60332641D1 (en)
ES (1) ES2346203T3 (en)
WO (1) WO2004029301A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1696040A1 (en) * 2005-02-28 2006-08-30 Eurofins Viralliance, Inc. Method of genotyping and phenotyping hepatitis B viruses resistant to antiviral molecules
CN101004415B (en) * 2007-01-16 2011-07-27 复旦大学附属华山医院 Method for measuring drug fast variation of hepatitis b virus to Adefovir
CN103114153B (en) * 2013-02-05 2015-09-02 西安交通大学医学院第一附属医院 A kind of fluorescent quantitative PCR detection method of duck hepatitis B virus and reagent
WO2015057919A1 (en) * 2013-10-18 2015-04-23 The Johns Hopkins University Transgenic hepatitis b virus (hbv): a new model of hbv infection identifies uqcr10 as a viral replication factor
WO2016120186A1 (en) 2015-01-27 2016-08-04 F. Hoffmann-La Roche Ag Recombinant hbv cccdna, the method to generate thereof and the use thereof
CN105907891A (en) * 2016-05-27 2016-08-31 北京旌准医疗科技有限公司 Method and kit for detecting HBV PgRNA in blood of patient with hepatitis b and application thereof
CN106555013A (en) * 2016-11-28 2017-04-05 北京旌准医疗科技有限公司 The detection method of HBV viruses, test kit and its application
CN106868045A (en) * 2017-02-24 2017-06-20 重庆市科学技术研究院 The construction method of Management of HBeAg negative mouse model
US20230323369A1 (en) 2020-06-24 2023-10-12 Xiamen University Screening model and method for hbv cccdna-targeting drug
CN112725535B (en) * 2021-02-03 2024-05-31 江苏安科华捷生物科技有限公司 Fluorescent quantitative PCR (polymerase chain reaction) kit for simultaneously detecting full-length and truncated HBV pgRNA (hepatitis B virus) and application thereof
CN114204358B (en) * 2021-12-13 2022-10-28 西安交通大学 Household direct current plug and socket with capacitor and working method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856459A (en) * 1995-06-06 1999-01-05 Hybridon, Inc. Oligonucleotides specific for hepatitis B virus

Family Cites Families (6)

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US5077192A (en) * 1988-10-25 1991-12-31 The General Hospital Corporation Method of detecting antigenic, nucleic acid-containing macromolecular entities
PT787200E (en) * 1994-10-28 2005-08-31 Univ Pennsylvania IMPROVED ADENOVIRUS AND METHODS FOR THEIR USE
US5723319A (en) * 1995-06-05 1998-03-03 Avid Therapeutics, Inc. Cultured cell line that inducibly expresses the hepatitis B virus genome, and uses thereof for screening antiviral substances
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
DE19822287C2 (en) * 1998-05-18 2003-04-24 Switch Biotech Ag Cloning vector, its production and use for the analysis of mRNA expression patterns
US6783940B2 (en) * 2001-10-31 2004-08-31 Applera Corporation Method of reducing non-specific amplification in PCR

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856459A (en) * 1995-06-06 1999-01-05 Hybridon, Inc. Oligonucleotides specific for hepatitis B virus

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BECK J ET AL: "Reconstitution of a functional duck hepatitis B virus replication initiation complex from separate reverse transcriptase domains expressed in Escherichia coli.", JOURNAL OF VIROLOGY. UNITED STATES AUG 2001, vol. 75, no. 16, August 2001 (2001-08-01), pages 7410 - 7419, XP002246285, ISSN: 0022-538X *
DATABASE EBI [online] 6 October 1999 (1999-10-06), FRANK, B.L., ET AL.: "Sequence 5 from patent US 5856459", XP002246286, accession no. EMBL Database accession no. AR027807 *
DATABASE GENBANK [online] 23 March 2001 (2001-03-23), SUGAUCHI, F. ET AL.: "Hepatitis B virus DNA, complete genome, serotype: ayw", XP002246287, retrieved from GI:13365548 accession no. NCBI Database accession no. AB048704 *
DELANEY W E 4TH ET AL: "Use of the hepatitis B virus recombinant baculovirus-HepG2 system to study the effects of (-)-beta-2',3'-dideoxy-3'-thiacytidine on replication of hepatitis B virus and accumulation of covalently closed circular DNA.", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. UNITED STATES AUG 1999, vol. 43, no. 8, August 1999 (1999-08-01), pages 2017 - 2026, XP002246282, ISSN: 0066-4804 *
DELMAS JULIEN ET AL: "Inhibitory effect of adefovir on viral DNA synthesis and covalently closed circular DNA formation in duck hepatitis B virus-infected hepatocytes in vivo and in vitro.", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. UNITED STATES FEB 2002, vol. 46, no. 2, February 2002 (2002-02-01), pages 425 - 433, XP002246284, ISSN: 0066-4804 *
GUNTHER STEPHAN ET AL: "Amplification of full-length hepatitis B virus genomes from samples from patients with low levels of viremia: Frequency and functional consequences of PCR-introduced mutations.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 36, no. 2, February 1998 (1998-02-01), pages 531 - 538, XP002246281, ISSN: 0095-1137 *
KRUINING JOHANNES ET AL: "Antiviral agents in hepatitis B virus transfected cell lines: Inhibitory and cytotoxic effect related to time of treatment.", JOURNAL OF HEPATOLOGY, vol. 22, no. 3, 1995, pages 263 - 267, XP002246283, ISSN: 0168-8278 *
SCHORIES MARCUS ET AL: "Isolation, characterization and biological significance of hepatitis B virus mutants from serum of a patient with immunologically negative HBV infection.", JOURNAL OF HEPATOLOGY, vol. 33, no. 5, November 2000 (2000-11-01), pages 799 - 811, XP002246280, ISSN: 0168-8278 *
ULRICH P P ET AL: "A PRECORE-DEFECTIVE MUTANT OF HEPATITIS B VIRUS ASSOCIATED WITH E ANTIGEN-NEGATIVE CHRONIC LIVER DISEASE", JOURNAL OF MEDICAL VIROLOGY, vol. 32, no. 2, 1990, pages 109 - 118, XP008019147, ISSN: 0146-6615 *

Also Published As

Publication number Publication date
EP1543168A2 (en) 2005-06-22
EP1543168B1 (en) 2010-05-19
CN100562586C (en) 2009-11-25
AU2003294704A1 (en) 2004-04-19
CA2500188A1 (en) 2004-04-08
ATE468411T1 (en) 2010-06-15
US20060240405A1 (en) 2006-10-26
DE60332641D1 (en) 2010-07-01
WO2004029301A2 (en) 2004-04-08
ES2346203T3 (en) 2010-10-13
CN1701124A (en) 2005-11-23
EP1403385A1 (en) 2004-03-31

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