WO2004029299A3 - Methods for detecting and differentiating bacteria - Google Patents

Methods for detecting and differentiating bacteria Download PDF

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Publication number
WO2004029299A3
WO2004029299A3 PCT/EP2003/010584 EP0310584W WO2004029299A3 WO 2004029299 A3 WO2004029299 A3 WO 2004029299A3 EP 0310584 W EP0310584 W EP 0310584W WO 2004029299 A3 WO2004029299 A3 WO 2004029299A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
detecting
bacteria
differentiating bacteria
differentiating
Prior art date
Application number
PCT/EP2003/010584
Other languages
German (de)
French (fr)
Other versions
WO2004029299A2 (en
Inventor
Michael Weizenegger
Michaela Bollen
Original Assignee
Hain Lifescience Gmbh
Michael Weizenegger
Michaela Bollen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hain Lifescience Gmbh, Michael Weizenegger, Michaela Bollen filed Critical Hain Lifescience Gmbh
Priority to AU2003270248A priority Critical patent/AU2003270248A1/en
Priority to EP03750608A priority patent/EP1543151A2/en
Publication of WO2004029299A2 publication Critical patent/WO2004029299A2/en
Publication of WO2004029299A3 publication Critical patent/WO2004029299A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention concerns the field of diagnosing microorganisms, particularly to the detection of bacteria. The invention particularly concerns hybridization methods and amplification methods as well as joint amplification/hybridization methods using sequence-specific probes or primers.
PCT/EP2003/010584 2002-09-24 2003-09-23 Methods for detecting and differentiating bacteria WO2004029299A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003270248A AU2003270248A1 (en) 2002-09-24 2003-09-23 Methods for detecting and differentiating bacteria
EP03750608A EP1543151A2 (en) 2002-09-24 2003-09-23 Methods for detecting and differentiating bacteria

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10244456.0 2002-09-24
DE10244456A DE10244456A1 (en) 2002-09-24 2002-09-24 Methods for the detection and differentiation of bacteria

Publications (2)

Publication Number Publication Date
WO2004029299A2 WO2004029299A2 (en) 2004-04-08
WO2004029299A3 true WO2004029299A3 (en) 2004-07-01

Family

ID=32009875

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/010584 WO2004029299A2 (en) 2002-09-24 2003-09-23 Methods for detecting and differentiating bacteria

Country Status (4)

Country Link
EP (1) EP1543151A2 (en)
AU (1) AU2003270248A1 (en)
DE (1) DE10244456A1 (en)
WO (1) WO2004029299A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9904804D0 (en) 1999-03-02 1999-04-28 King S College London Identification of bacteria
DK1709198T3 (en) * 2003-11-26 2013-09-02 Advandx Inc Peptide Nucleic Acid Probes for the Analysis of Certain Staphylococcus Species
JP4874999B2 (en) 2005-02-07 2012-02-15 ジェン−プローブ・インコーポレーテッド Compositions and methods for detecting group B Streptococcus
EP2106450A4 (en) * 2007-01-08 2010-03-10 Medigenes Co Ltd Dna chip for detection of escherichia coli
EP2183391A4 (en) 2007-07-31 2010-10-27 Quest Diagnostics Invest Inc Detection of methicillin-resistant and methicillin-sensitive staphylococcus aureus in biological samples
FI121884B (en) * 2008-01-17 2011-05-31 Mobidiag Oy Method for detection and identification of methicillin-resistant staphylococci, probe and test kit for the method

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015157A1 (en) * 1989-05-31 1990-12-13 Gene-Trak Systems Universal eubacteria nucleic acid probes and methods
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
WO1998020157A2 (en) * 1996-11-04 1998-05-14 Infectio Diagnostic (I.D.I.) Inc. Species-specific, genus-specific and universal dna probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
WO1999024613A1 (en) * 1997-11-06 1999-05-20 Pathobiotek Inc. Human blood bacterium
US6150517A (en) * 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
US6221635B1 (en) * 1999-05-06 2001-04-24 The Wistar Institute Methods for solid-phase amplification of DNA template (SPADT) using multiarrays
WO2001034842A2 (en) * 1999-11-12 2001-05-17 University Of Chicago Pcr amplification on microarrays of gel immobilized oligonucleotides
WO2001036683A2 (en) * 1999-11-16 2001-05-25 Apollo Biotechnology, Inc. Method for rapid and accurate identification of microorganisms
WO2001066797A2 (en) * 2000-03-03 2001-09-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Multiplex hybridization system for identification of pathogenic mycobacterium and method of use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001564A (en) * 1994-09-12 1999-12-14 Infectio Diagnostic, Inc. Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
EP1075546A1 (en) * 1998-05-04 2001-02-14 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method and device for isolating nucleic acids
DE19819889A1 (en) * 1998-05-04 1999-11-11 Fraunhofer Ges Forschung Isolating nucleic acid from samples by binding to array of immobilized, random capture probes
DE10004147A1 (en) * 2000-01-31 2001-08-09 Gsf Forschungszentrum Umwelt Oligonucleotides for the amplification and qualitative and quantitative detection of bacterial 16S rRNA useful to determine bacterial contamination of foodstuffs, waters, and in clinical microbiology

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150517A (en) * 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
WO1990015157A1 (en) * 1989-05-31 1990-12-13 Gene-Trak Systems Universal eubacteria nucleic acid probes and methods
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
WO1998020157A2 (en) * 1996-11-04 1998-05-14 Infectio Diagnostic (I.D.I.) Inc. Species-specific, genus-specific and universal dna probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
WO1999024613A1 (en) * 1997-11-06 1999-05-20 Pathobiotek Inc. Human blood bacterium
US6221635B1 (en) * 1999-05-06 2001-04-24 The Wistar Institute Methods for solid-phase amplification of DNA template (SPADT) using multiarrays
WO2001034842A2 (en) * 1999-11-12 2001-05-17 University Of Chicago Pcr amplification on microarrays of gel immobilized oligonucleotides
WO2001036683A2 (en) * 1999-11-16 2001-05-25 Apollo Biotechnology, Inc. Method for rapid and accurate identification of microorganisms
WO2001066797A2 (en) * 2000-03-03 2001-09-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Multiplex hybridization system for identification of pathogenic mycobacterium and method of use

Also Published As

Publication number Publication date
EP1543151A2 (en) 2005-06-22
AU2003270248A8 (en) 2004-04-19
DE10244456A1 (en) 2004-04-15
WO2004029299A2 (en) 2004-04-08
AU2003270248A1 (en) 2004-04-19

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