WO2004029215A3 - Conditional gene expression using rnai - Google Patents

Conditional gene expression using rnai Download PDF

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Publication number
WO2004029215A3
WO2004029215A3 PCT/US2003/030531 US0330531W WO2004029215A3 WO 2004029215 A3 WO2004029215 A3 WO 2004029215A3 US 0330531 W US0330531 W US 0330531W WO 2004029215 A3 WO2004029215 A3 WO 2004029215A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene expression
rnai
conditional gene
rnai response
inhibiting
Prior art date
Application number
PCT/US2003/030531
Other languages
French (fr)
Other versions
WO2004029215A2 (en
WO2004029215A9 (en
Inventor
Cynthia Kenyon
Andrew Dillin
Coleen Murphy
Original Assignee
Univ California
Cynthia Kenyon
Andrew Dillin
Coleen Murphy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Cynthia Kenyon, Andrew Dillin, Coleen Murphy filed Critical Univ California
Priority to AU2003277005A priority Critical patent/AU2003277005A1/en
Publication of WO2004029215A2 publication Critical patent/WO2004029215A2/en
Publication of WO2004029215A9 publication Critical patent/WO2004029215A9/en
Publication of WO2004029215A3 publication Critical patent/WO2004029215A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to regulation of gene expression and conditional gene expression by regulating an RNAi response. More particularly, the present invention is directed to methods of inhibiting an RNAi response by contacting a cell with a dsRNA directed against a component of the RNAi response, e.g., with a dcr-1 dsRNA, thereby inhibiting an RNAi response.
PCT/US2003/030531 2002-09-26 2003-09-26 Conditional gene expression using rnai WO2004029215A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003277005A AU2003277005A1 (en) 2002-09-26 2003-09-26 Conditional gene expression using rnai

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41379402P 2002-09-26 2002-09-26
US60/413,794 2002-09-26

Publications (3)

Publication Number Publication Date
WO2004029215A2 WO2004029215A2 (en) 2004-04-08
WO2004029215A9 WO2004029215A9 (en) 2004-06-03
WO2004029215A3 true WO2004029215A3 (en) 2004-09-23

Family

ID=32043291

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/030531 WO2004029215A2 (en) 2002-09-26 2003-09-26 Conditional gene expression using rnai

Country Status (2)

Country Link
AU (1) AU2003277005A1 (en)
WO (1) WO2004029215A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070265220A1 (en) 2004-03-15 2007-11-15 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
WO2005089287A2 (en) * 2004-03-15 2005-09-29 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded rna
CN109112117B (en) * 2017-09-04 2020-11-27 华中农业大学 Separated chilo suppressalis CYP15C1 gene and encoded protein thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
RUVKUN, G.: "Glimpses of a tiny RNA world", SCIENCE, vol. 294, 26 October 2001 (2001-10-26), pages 797 - 799, XP002978225 *
TIJSTERMAN ET AL: "RNA helicase MUT-14-dependent gene silencing triggered in C. elegans by short antisense RNAs", SCIENCE, vol. 295, 25 January 2002 (2002-01-25), pages 694 - 697, XP002257818 *

Also Published As

Publication number Publication date
WO2004029215A2 (en) 2004-04-08
AU2003277005A8 (en) 2004-04-19
WO2004029215A9 (en) 2004-06-03
AU2003277005A1 (en) 2004-04-19

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