WO2004028535A1 - Pin1-modulating compounds and methods of use thereof - Google Patents

Pin1-modulating compounds and methods of use thereof Download PDF

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Publication number
WO2004028535A1
WO2004028535A1 PCT/US2003/006675 US0306675W WO2004028535A1 WO 2004028535 A1 WO2004028535 A1 WO 2004028535A1 US 0306675 W US0306675 W US 0306675W WO 2004028535 A1 WO2004028535 A1 WO 2004028535A1
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group
pinl
compound
derivatives
cancer
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PCT/US2003/006675
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French (fr)
Inventor
Timothy D. Mckee
Robert K. Suto
Thomas Tibbitts
Janusz Sowadski
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Pintex Pharmaceuticals, Inc.
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Priority to EP03798653A priority Critical patent/EP1551396A1/en
Priority to AU2003225669A priority patent/AU2003225669A1/en
Publication of WO2004028535A1 publication Critical patent/WO2004028535A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/425—Thiazoles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole

Definitions

  • PPIases The peptidyl-prolyl cis-trans isomerases (PPIases), or rotamases, are a family of ubiquitous enzymes that catalyze the cis/trans isomerization of the peptide bond on the N- terminal side of proline residues in proteins (Hunter, Cell 92:141-142, 1998). PPIases are divided into three classes, cyclophilins (Cyps), FK-506 binding proteins (FKBPs) and the Pinl/parvulin class. Cyclophilins and FKBPs are distinguished by their ability to bind the clinically immunosuppressive drugs cyclosporin and FK506, respectively (Schreiber, Science 251:283-7, 1991; Hunter, supra).
  • Pinsl-3 (Lu et al., Nature 380;544-547, 1996)
  • Pin-L Campbell et al., Genomics 44:157-162, 1997)
  • parvulin (Rahfeld, et al., Proc. Natl. Acad. Sci. USA 93:447-451, 1996)
  • Essl/Pftl Hanes et al., Yeast 5:55- 72, 1989; and Hani, et al. FEBS Letts 365:198-202, 1995).
  • Pinl is a highly conserved protein that catalyzes the isomerization of only phosphorylated Ser/Thr-Pro bonds (Rananathan, R. et al. (1997) Cell 89:875-86; Yaffe, et al. 1997, Science 278:1957-1960; Shen, et al. 1998,Genes Dev. 12:706-720; Lu, et al. 1999, Science 283:1325-1328; Crenshaw, et al. 1998, Embo J. 17:1315-1327; Lu, et al. 1999, Nature 399:784-788; Zhou, et al. 1999, Cell Mol. Life Sci. 56:788-806).
  • Pinl contains an N-terminal WW domain, which functions as a phosphorylated Ser/Thre-Pro binding module (Sudol, M. (1996) Prog. Biophys. Mol. Biol. 65:113-32).
  • This phosphorylation-dependent interaction targets Pinl to a subset of phosphorylated substrates, including Cdc25, Wee 1, Mytl, Tau-Rad4, and the C-terminal domain of RNA polymerase II large domain (Crenshaw, D.G., et al (1998) Embo. J. 17:1315-27; Shen, M. (1998) Genes Dev. 12:706-20; Wells, NJ. (1999) J. Cell. Sci. 112: 3861-71).
  • Pinl activity is essential for cell growth; depletion or mutations of Pinl cause growth arrest, affect cell cycle checkpoints and induce premature mitotic entry, mitotic arrest and apoptosis in human tumor cells, yeast or Xenopus extracts (Lu, et al. 1996, Nature 380:544-547; Winkler, et al. 200, Science 287:1644-1647; Hani, et al. 1999. J. Biol. Chem. 274:108-116).
  • Pinl is dramatically overexpressed in human cancer samples and the levels of Pinl are correlated with the aggressiveness of tumors.
  • Pinl antisense polynucleotides or genetic depletion kills human and yeast dividing cells by inducing premature mitotic entry and apoptosis.
  • Pinl -catalyzed prolyl isomerization regulates the conformation and function of these phosphoprotein substrates and facilitates dephosphorylation because of the conformational specificity of some phosphatases.
  • Pinl -dependent peptide bond isomerization is a critical post-phosphorylation regulatory mechanism, allowing cells to turn phosphoprotein function on or off with high efficiency and specificity during temporally regulated events, including the cell cycle (Lu et al, supra).
  • the invention is directed to modulators, e.g., inhibitors, of Pinl and Pinl -related proteins and the use of such modulators for treatment of Pinl associated states, e.g., for the treatment of cancer.
  • the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I):
  • Z and Zi are independently selected from the group consisting of O or S;
  • AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O) 2 O-, -S-, or -OCH 2 -, phosphoprotein function on or off with high efficiency and specificity during temporally regulated events, including the cell cycle (Lu et al, supra).
  • the invention is directed to modulators, e.g., inhibitors, of Pinl and Pinl-related proteins and the use of such modulators for treatment of Pinl associated states, e. g. , for the treatment of cancer.
  • the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I):
  • Z and Zi are independently selected from the group consisting of O or S;
  • AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O) 2 O-, -S-, or -OCH 2 -, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH 3 , OEt, NO 2 , CO 2 H, CI, OH, F, Br, OCH 3 , CF 3 , OCF 3 , and - SO 2 CF 3 , OAc, -O-iBu, -S(O) 2 NH 2 , -CHO, C(O)CH 3 , -CN, CO 2 (CH 2 ) q CH 3 , wherein q is an integer ranging from about 0 to 4, and any combination thereof; ⁇
  • Ri is selected from the group consisting of H; -(X) p C(O)R 2 , wherein p is selected from the group consisting of 1 through 4, wherein X is CH 2 or NH and which may be substituted with benzyl, wherein R 2 is selected from the group consisting of OH, NR 3 and phenyl, and wherein R 3 is N-thiazol-2-yl-benzenesulfonamide; CH 3 ; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; such that the Pinl -associated state is treated.
  • the invention pertains, at least in part, to a method for treating cyclin Dl overexpression in a subject.
  • This method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I):
  • n is selected from the group consisting of 0 rough 10; m is O or 1;
  • Z and Zi are independently selected from the group consisting of O or
  • AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O) 2 O-, -S-, or -OCH 2 L , wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH 3 , OEt, NO 2 , CO 2 H, CI, OH, F, Br, OCH 3 , CF 3 , OCF 3 , and - SO 2 CF 3 , OAc, -O-iBu, -S(O) 2 NH 2 , -CHO, C(O)CH 3 , -CN, CO 2 (CH 2 ) q CH 3 , wherein q is an integer ranging from about 0 to 4, and any combination thereof;
  • Ri is selected from the group consisting of H; -(X) p C(O)R 2 , wherein p is selected from the group consisting of 1 through 4, wherein X is CH 2 or NH and which may be substituted with benzyl, wherein R 2 is selected from the group consisting of OH, NR 3 and phenyl, and wherein R 3 is N-thiazol-2-yl-benzenesulfonamide; CH 3 ; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; packaged with instructions for using an effective amount of the Pinl- modulating compound to treat a Pinl associated state.
  • the invention also includes a packaged cyclin Dl overexpression treatment.
  • This packaged treatment include a Pinl -modulating compound of formula (I):
  • Z and Zi are independently selected from the group consisting of O or
  • AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O) 2 O-, -S-, or -OCH 2 -, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH 3 , OEt, NO 2 , CO 2 H, CI, OH, F, Br, OCH 3 , CF 3 , OCF 3 , and -
  • R1 is selected from the group consisting of H; -(X) p C(O)R 2 , wherein p is selected from the group consisting of 1 through 4, wherein X is CH 2 or NH and which may be substituted with benzyl, wherein R 2 is selected from the group consisting of OH, NR 3 and phenyl, and wherein R 3 is N-thiazol-2-yl-benzenesulfonamide; CH 3 ; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; packaged with instructions for using an effective amount of the Pinl -modulating compound to treat cancer.
  • the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject.
  • the method includes administering to a subject an effective amount of a combination of a Pinl -modulating compound of formula (I):
  • Z and Zi are independently selected from the group consisting of O or S;
  • AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O) 2 ⁇ -, -S-, or -OCH 2 -, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH 3 , OEt, NO 2 , CO 2 H, CI, OH, F, Br, OCH 3 , CF 3 , OCF 3 , and -
  • Ri is selected from the group consisting of H; - X) p C(O)R 2 , wherein p is selected from the group consisting of 1 through 4, wherein X is CH 2 or NH and which may be substituted with benzyl, wherein 2 is selected from the group consisting of OH, NR 3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH 3 ; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; and a hyperplastic inhibitory agent such that the Pinl associated state is treated.
  • the invention pertains, at least in part, to a method for treating cancer in a subject. The method includes administering to the subject an effective amount of a combination of a Pin
  • n is selected from the group consisting of 0 through 10; m is O or l;
  • Z and Zi are independently selected from the group consisting of O or S;
  • AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O) 2 ⁇ -, -S-, or -OCH 2 -, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH 3 , OEt, NO 2 , CO 2 H, CI, OH, F, Br, OCH 3 , CF 3 , OCF 3 , and - SO 2 CF 3 , OAc, -O-iBu, -S(O) NH 2 , -CHO, C(O)CH 3 , -CN, CO 2 (CH 2 ) q CH 3 , wherein q is an
  • Rt is selected from the group consisting of H; -(X) p C(O)R 2 , wherein p is selected from the group consisting of 1 through 4, wherein X is CH 2 or NH and which may be substituted with benzyl, wherein R 2 is selected from the group consisting of OH, NR 3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH 3 ; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; and a hyperplastic inhibitory agent such that the cancer is treated.
  • the invention is a method for treating cyclin Dl overexpression in a subject.
  • the method includes administering to the subject an effective amount of a combination of a Pinl -modulating compound of formula (I):
  • n is selected from the group consisting of 0 through 10; m is 0 or 1 ;
  • Z and Zi are independently selected from the group consisting of O or
  • AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O) 2 ⁇ -, -S-, or -OCH 2 -, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH 3 , OEt, NO 2 , CO 2 H, CI, OH, F, Br, OCH 3 , CF 3 , OCF 3 , and -
  • -8- Ri is selected from the group consisting of H; -(X) p C(O)R 2 , wherein p is selected from the group consisting of 1 through 4, wherein X is CH 2 or NH and which may be substituted with benzyl, wherein R 2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R 3 isN-thiazol-2-yl-benzenesulfonamide; CH 3 ; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide.
  • Another embodiment of the invention is a pharmaceutical composition
  • a pharmaceutical composition comprising a Pinl -modulating compound as prepared according to the methodology of this invention, and a pharmaceutically acceptable carrier.
  • the invention is directed to modulators, e.g., inhibitors, of Pinl and Pinl- related proteins and the use of such modulators for treatment of Pinl associated states, e.g., for the treatment of cancer.
  • the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject.
  • the method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I):
  • Z and Zi are independently selected from the group consisting of O or
  • -9- AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O) 2 O-, -S-, or -OCH 2 -, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH 3 , OEt, NO 2 , CO 2 H, CI, OH, F, Br, OCH 3 , CF 3 , OCF 3 , and - SO 2 CF 3 , OAc, -O-iBu, -S(O) 2 NH 2 , -CHO, C(O)CH 3 , -CN, CO 2 (CH 2 ) q CH 3 , wherein q is an integer ranging from about 0 to 4, and any combination thereof;
  • Ri is selected from the group consisting of H; -(X) p C(O)R 2 , wherein p is selected from the group consisting of 1 through 4, wherein X is CH 2 or NH and which may be substituted with benzyl, wherein R 2 is selected from the group consisting of OH, NR 3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; such that the Pinl -associated state is treated.
  • the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to the subject an effective amount of a Pinl -associated
  • R 2 , R 3 , and Re are independently selected from the group consisting of
  • R 4 is H or lower alkyl, e.g., CH3;
  • Xi, X 2 and X3 are independently selected from the group consisting of
  • the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject.
  • the method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (III):
  • R 2 , R 3 , and R are independently selected from the group consisting of H, OCH 3 , SO 2 CF 3 , -S(O 2 )NH 2 , OH, CI, C(O)CH 3 , -CN, NO 2 , F, CF 3 , - OCF 3 , CO 2 H, CO 2 (CH 2 ) q CH 3 , CH 3 , and Br, wherein q is an integer ranging from about 0 to 4; R 5 is H or lower alkyl, e.g., CH 3 ;
  • Pinl -associated states include states resulting from an elevation in the expression of cyclin Dl and or Pinl.
  • Pinl-associated states also include states resulting from an elevation in the phosphorylation level of c-Jun, particularly phosphorylation of c-Jun on Ser 63/73 -Pro and/or from an elevation in the level of c-Jun amino terminal kinases (JNKs) present in a cell.
  • Pinl- associated states include neoplasia, cancer, undesirable cell growth, and/or tumor growth.
  • Pinl-associated states include states caused by DNA damage, an oncogenic protein (i.e.
  • Pinl is an important regulator of cyclin Dl expression. Due to Pinl 's role in regulating the expression of cyclin D 1 , many of the tumor causing effects of cyclin D 1 can be regulated through Pinl. In particular, inhibitors of Pinl can also be used to treat, inhibit, and/or prevent undesirable cell growth, e.g., tumors, neoplasia, and/or cancer associated with aberrant cyclin Dl expression in a subject.
  • undesirable cell growth e.g., tumors, neoplasia, and/or cancer associated with aberrant cyclin Dl expression in a subject.
  • Pinl associated states include, but are not limited to, for example, those tumor types disclosed in Table 8.
  • treatment includes the diminishment or alleviation of at least one symptom associated or caused by the state, disorder or disease being treated.
  • the treatment comprises the induction of a Pinl inhibited state, followed by the activation of the Pinl modulating compound, which would in turn diminish or alleviate at least one symptom associated or caused by the Pinl associated state, disorder or disease being treated.
  • treatment can be diminishment of one or several symptoms of a disorder or complete eradication of a disorder.
  • subject is intended to include organisms, e.g., prokaryotes and eukaryotes, which are capable of suffering from or afflicted with a Pinl associated disorder.
  • subjects include mammals, e.g. , humans, dogs, cows, horses, pigs, sheep, goats, cats, mice, rabbits, rats, and transgenic non-human animals.
  • the subject is a human, e.g., a human suffering from, at risk of suffering from, or potentially capable of suffering from a Pinl associated disorder.
  • Pinl modulating compound refers to compounds that modulate, e.g., inhibit, promote, or otherwise alter, the activity of Pinl.
  • Pinl modulating compounds include both Pinl agonists and antagonists.
  • the Pinl modulating compound induces a Pinl inhibited-state.
  • Examples of Pinl modulating compounds include compounds of formula (I), formula (II), and formula (III). Additional examples of Pinl modulating compounds include compounds of Table 1, Table 2, Table 3, Table 4, Table 5, Table 6, or derivatives thereof.
  • the Pinl modulating compounds include compounds that interact with the PPI or the WW domain of
  • the Pinl modulating compound is substantially specific to Pinl.
  • the phrase "substantially specific for Pinl” is intended to include inhibitors of the invention that have a Kj or K that is at least 2, 3, 4, 5, 10, 15, or 20 times less than the Ki or K for other peptidyl prolyl isomerases, e.g., hCyP-A, hCyP-B, hCyP-C, NKCA, hFKBP-12, hFKBP-13, and hFKBP-25.
  • the Pinl modulating compound of the invention is capable of chemically interacting with Cysll3 of Pinl.
  • the language "chemical interaction" is intended to include, but is not limited to reversible interactions such as hydrophobic/hydrophilic, ionic (e.g., coulombic attraction/repulsion, ion-dipole, charge- transfer), covalent bonding, Nan der Waals, and hydrogen bonding.
  • the chemical interaction is a reversible Michael addition.
  • the Michael addition involves, at least in part, the formation of a covalent bond.
  • Pinl inhibiting compound includes compounds that reduce or inhibit the activity of Pinl.
  • Examples of Pinl inhibiting compounds include compounds of formula (I), formula (II), and formula (III). Additional examples of Pinl inhibiting compounds include compounds of Table 1, Table 2, Table 3, Table 4, Table 5, Table 6, or derivatives thereof.
  • the Pinl inhibiting compounds include compounds that interact with the PPI or the WW domain of Pinl .
  • the inhibitors have a Ks for Pinl of less than 0.2mM, less than O.lmM, less than 750 ⁇ M, less than 500 ⁇ M, less than 250 ⁇ M, less than 100 ⁇ M, less than 50 ⁇ M, less than 500 nM, less than 250nM, less than 50 nM, less than 10 nM, less than 5 nM, or or less than 2 nM.
  • Pinl inhibited-state is intended to include states in which one activity of Pinl is inhibited in cells, e.g., cells in a subject, that have been treated with a Pinl modulating compound.
  • Pinl inbited-state is also intended to include states wherein the Pinl modulating compound is administered to a subject, allowed to remain in a preactivated state, and subsequently activated by a stimulus.
  • the stimulus may be selected from a natural event, artificial event, or the combination thereof.
  • the natural event may be the action of an enzyme and/or the artificial event may be the addition of a hyperplastic inhibitory agent or the addition of energy to the subjects system in any manner that achieves activation, e.g.
  • the cells enter a Pinl inhibited-state for a designated period of time prior to activation of the modulating compound sufficient to allow the modulation the activity of Pinl by the activated modulating compound.
  • the Pinl modulating compound is preactivated prior to administration to a subject followed by the introduction of at least one stimulus sufficient to allow the modulation the activity of Pinl by the modulating compound.
  • the activity of the modulating compound is enhanced by the entrance of the cells, e.g. , cells of a subject, into a Pinl inhibited state.
  • the Pinl modulating compounds of the invention have a characteristic inhibition profile (CIP) and have an effective cytotoxicity, e.g., effective to treat a Pinl associated state.
  • CIP characteristic inhibition profile
  • the Pinl -modulating compounds described herein may be substituted with any substituent that allows the Pinl -modulating compound to perform its intended function.
  • the Pinl -modulating compounds described herein may be substituted with any substituent which allows the Pinl -modulating compound to perform its intended function, possess a CIP, and/or be effectively cytotoxic, as defined herein.
  • the cytotoxicity of the compounds can be determined by using the CPCA given in Example 1.
  • the measurement of the activity of the Pinl -modulating compounds in the determination the inhibition constant at 50% inhibition of enzyme activity (IC 50 ), which is used to characterize the CIP, may be performed by using the analysis described in Example 2.
  • An ordinarily skilled artisan would be able to use data generated by the assays to modify substituents on the Pinl modulating compounds to obtain effectively cytotoxic Pinl modulating compounds with characteristic inhibition profiles.
  • Characteristic inhibition profile is a characterization of the modulating compound of the invention such that the Pinl-associated state is inhibited. Characterization of the modulating compounds includes measurement of the inhibition constant at 50% inhibition of enzyme activity (IC 50 ).
  • Compounds that demonstrate a CIP include modulating compounds with and ICso of less than about 40 ⁇ M. In certain embodiments of the invention, the IC 5 0 is between about 10-40 ⁇ M. In additional embodiments, the IC 50 is between about 1-10 ⁇ M. In certain embodiments, the ICso is less than about 1 ⁇ M.
  • cytotoxicity or "effectively cytotoxic” includes cytotoxicities of Pinl -modulating compounds which allow the Pinl -modulating compound to perform its intended function, e.g., treat Pinl associated states. Cytotoxicities can be measured, for example, by using the Cell Based Cytotoxicity Assay (CBCA) method described in Example 1.
  • CBCA Cell Based Cytotoxicity Assay
  • the Pinl -modulating compound has a
  • -14- cytotoxicity (as measured by the CBCA in Example 1) of 50 ⁇ M or less, 45 ⁇ M or less, 40 ⁇ M or less, 35 ⁇ M or less, 30 ⁇ M or less, 25 ⁇ M or less, 20 ⁇ M or less, 15 ⁇ M or less, 10 ⁇ M or less, 9 ⁇ M or less, 8 ⁇ M or less, 7 ⁇ M or less, 6 ⁇ M or less, 5 ⁇ M or less, 4 ⁇ M or less, 3 ⁇ M or less, 2 ⁇ M or less, 1 ⁇ M or less, 0.9 ⁇ M or less, 0.8 ⁇ M or less, 0.7 ⁇ M or less, 0.6 ⁇ M or less, 0.5 ⁇ M or less, 0.4 ⁇ M or less, or, preferably, 0.3 ⁇ M or less, or 0.05 ⁇ M or less. Values and ranges included and/or intermediate of the values set forth herein are also intended to be within the scope of the present invention.
  • the Pinl modulating compounds of the invention are substantially soluble, e.g., water soluble, and have an effective cytotoxicity, e.g., effective to treat a Pinl associated state.
  • Methods for altering the solubility of organic compounds are known in the art. For example, one of ordinary skill in the art will be able to modify the Pinl modulating compounds of the invention such that they have a desirable logP. Ordinarily skilled artisans will be able to modify the compounds by adding and removing hydrophilic and hydrophobic moieties, such that a Pinl -modulating compound with a desired solubility is obtained.
  • the Pinl -modulating compounds described herein may be substituted with any substituent which allows the Pinl -modulating compound to perform its intended function, be substantially soluble, and/or be effectively cytotoxic, as defined herein.
  • any substituent which allows the Pinl -modulating compound to perform its intended function be substantially soluble, and/or be effectively cytotoxic, as defined herein.
  • an ordinarily skilled artisan would understand that the addition of heteroatoms (hydroxy, amino, nitro, carboxylic acid groups, etc.) or other polar moieties would generally increase the solubility of the Pinl modulating compound in water, while addition of non-polar moieties such as aryl or alkyl groups would generally decrease the solubility of the compound in water.
  • the Pinl modulating compound can then be tested for substantial solubility by determining the logP value (e.g., by using a log octanol- water partition coefficient program such as "KOWWIN” (Meylan, W.M. and P.H. Howard. 1995. Atom/fragment contribution method for estimating octanol-water partition coefficients. J. Pharm. Sci. 84: 83-92, incorporated herein by reference in its entirety).
  • KWWIN log octanol- water partition coefficient program
  • An ordinarily skilled artisan would be able to use data generated by these programs and assays to modify substituents on the Pinl modulating compounds to obtain substantially soluble and effectively cytotoxic Pinl modulating compounds.
  • substantially soluble includes solubilities (e.g., aqueous solubilities) of
  • Pinl -modulating compounds that allow the Pinl -modulating compounds to perform their intended function, e.g., treat Pinl associated states.
  • the solubility of a particular Pinl- modulating compound can be measured by any method known in the art, e.g., experimentally, computationally, etc.
  • one method for determining the solubility of a compound computationally is by calculating logP values using a log octanol-water partition coefficient program (KOWWIN).
  • the Pinl -modulating compounds of the invention is by calculating logP values using a log octanol-water partition coefficient program (KOWWIN).
  • the Pinl -modulating compounds of the invention may have a logP value between about 1 to about 6, between about 1 to about 5, between about 1.5 to about 5, between about 2 to about 5, between about 2.5 to about 4.5, between about 2.75 to about 4.25, between about 3.0 to about 4.0, between about 3.25 to about 4.0, between about 3.5 to about 4.0, and between about 3.5 to about 3.75. Values and ranges included and/or intermediate of the values set forth herein are also intended to be within the scope of the present invention.
  • the aqueous solubility of the compound is about 0.01 mg/L or greater, about 0.1 mg/L or greater, about 1 mg/L or greater, or about 2 mg/L or greater.
  • Z is S.
  • Z ⁇ is
  • n is selected from the group consisting of 0 through 5.
  • the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3 -(methylene)-l -methyl- 1,3 -dihydro-indol- 2-one, a benzofl ,3]dioxole, and Furazan 2-oxide.
  • derivative is intended to include isomers, modification, e.g., addition or removal, of substituents on the Pinl -modulating compound, and pharmaceutically acceptable salts thereof, as well as formulation, such that the Pinl -modulating compound treats the Pinl -associated state.
  • alkyl includes saturated aliphatic groups, including straight-chain alkyl groups (e.g., methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, etc.), branched-chain alkyl groups (isopropyl, tert-butyl, isobutyl, etc.), cycloalkyl (alicyclic) groups (cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl), alkyl substituted cycloalkyl groups, and cycloalkyl substituted alkyl groups.
  • straight-chain alkyl groups e.g., methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl,
  • alkyl further includes " alkyl groups, which can further include oxygen, nitrogen, sulfur or phosphorous atoms replacing one or more carbons of the hydrocarbon backbone.
  • a straight chain or branched chain alkyl has 10 or fewer carbon atoms in its backbone (e.g., Cr o for straight chain, C 3 -C 10 for branched chain), and more preferably 6 or fewer.
  • preferred cycloalkyls have from 4-7 carbon atoms in their ring structure, and more preferably have 5 or 6 carbons in the ring structure.
  • alkyl includes both "unsubstituted alkyls" and “substituted alkyls", the latter of which refers to alkyl moieties having substituents replacing a hydrogen on one or more carbons of the hydrocarbon backbone.
  • substituents can include, for example, alkenyl, alkynyl, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl,
  • alkylthiocarbonyl alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, frifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moiety.
  • Cycloalkyls can be further substituted, e.g., with the substituents described above.
  • An "alkylaryl” or an “aralkyr' moiety is an alkyl substituted with an aryl (e.g., phenylmethyl (benzyl)).
  • the term “alkyl” also includes the side chains of natural and unnatural amino acids. Examples of halogenated alkyl groups include fluoromethyl, difluoromethyl, trifluoromethyl, chloromethyl, dichloromethyl, trichloromethyl, perfluoromethyl, perchloromethyl, perfluoroethyl, perchloroethyl, etc.
  • aryl includes groups, including 5- and 6-membered single-ring aromatic groups that may include from zero to four heteroatoms, for example, benzene, phenyl, pyrrole, furan, thiophene, thiazole, isothiaozole, imidazole, triazole, tetrazole, pyrazole, oxazole, isooxazole, pyridine, pyrazine, pyridazine, and pyrimidine, and the like.
  • aryl includes multicyclic aryl groups, e.g., tricyclic, bicyclic, e.g., naphthalene, benzoxazole, benzodioxazole, benzothiazole, benzoimidazole, benzothiophene, methylenedioxyphenyl, quinoline, isoquinoline, napthridine, indole, benzofuran, purine, benzofuran, deazapurine, or indolizine.
  • aryl groups having heteroatoms in the ring structure may also be referred to as “aryl heterocycles", “heterocycles,” “heteroaryls” or “heteroaromatics”.
  • the aromatic ring can be substituted at one or more ring positions with such substituents as described above, as for example, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, aralkylaminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylthiocarbonyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and
  • alkenyl includes unsaturated aliphatic groups analogous in length and possible substitution to the alkyls described above, but which contain at least one double bond.
  • alkenyl includes straight-chain alkenyl groups (e.g., ethenyl, propenyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, nonenyl, decenyl, etc.), branched-chain alkenyl groups, cycloalkenyl (alicyclic) groups (cyclopropenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl, cyclooctenyl), alkyl or alkenyl substituted cycloalkenyl groups, and cycloalkyl or cycloalkenyl substituted alkenyl groups.
  • alkenyl includes straight-chain alkenyl groups (e.g., ethenyl, propenyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, nonenyl, de
  • alkenyl further includes alkenyl groups that include oxygen, nitrogen, sulfur or phosphorous atoms replacing one or more carbons of the hydrocarbon backbone.
  • a straight chain or branched chain alkenyl group has 6 or fewer carbon atoms in its backbone (e.g., C 2 -C 6 for straight chain, C 3 -C 6 for branched chain).
  • cycloalkenyl groups may have from 3-8 carbon atoms in their ring structure, and more preferably have 5 or 6 carbons in the ring structure.
  • C 2 -C 6 includes alkenyl groups containing 2 to 6 carbon atoms.
  • alkenyl includes both "unsubstituted alkenyls" and “substituted alkenyls”, the latter of which refers to alkenyl moieties having substituents replacing a hydrogen on one or more carbons of the hydrocarbon backbone.
  • substituents can include, for example, alkyl groups, alkynyl groups, halogens, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate,
  • alkynyl includes unsaturated aliphatic groups analogous in length and possible substitution to the alkyls described above, but which contain at least one triple bond.
  • alkynyl includes straight-chain alkynyl groups (e.g., ethynyl, propynyl, butynyl, pentynyl, hexynyl, heptynyl, octynyl, nonynyl, decynyl, etc.), branched-chain alkynyl groups, and cycloalkyl or cycloalkenyl substituted alkynyl groups.
  • alkynyl further includes alkynyl groups that include oxygen, nitrogen, sulfur or phosphorous atoms replacing one or more carbons of the hydrocarbon backbone.
  • a straight chain or branched chain alkynyl group has 6 or fewer carbon atoms in its backbone (e.g., C 2 -C6 for straight chain, C 3 -C 6 for branched chain).
  • C 2 -C 6 includes alkynyl groups containing 2 to 6 carbon atoms.
  • alkynyl includes both "unsubstituted alkynyls" and
  • substituted alkynyls the latter of which refers to alkynyl moieties having substituents
  • substituents can include, for example, alkyl groups, alkynyl groups, halogens, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, al
  • lower alkyl as used herein means an alkyl group, as defined above, but having from one to five carbon atoms in its backbone structure.
  • Lower alkenyl and “lower alkynyl” have chain lengths of, for example, 2-5 carbon atoms.
  • acyl includes compounds and moieties which contain the acyl radical (CH 3 CO-) or a carbonyl group.
  • substituted acyl includes acyl groups where one or more of the hydrogen atoms are replaced by for example, alkyl groups, alkynyl groups, halogens, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydry
  • acylamino includes moieties wherein an acyl moiety is bonded to an amino group.
  • the term includes alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido groups.
  • aroyi includes compounds and moieties with an aryl or heteroaromatic moiety bound to a carbonyl group. Examples of aroyl groups include phenylcarboxy, naphthyl carboxy, etc.
  • alkoxyalkyl include alkyl groups, as described above, which further include oxygen, nitrogen or sulfur atoms replacing one or more carbons of the hydrocarbon backbone, e.g., oxygen, nitrogen or sulfur atoms.
  • alkoxy includes substituted and unsubstituted alkyl, alkenyl, and alkynyl groups covalently linked to an oxygen atom.
  • alkoxy groups include methoxy, ethoxy, isopropyloxy, propoxy, butoxy, and pentoxy groups and may include cyclic groups such as cyclopentoxy.
  • substituted alkoxy groups include halogenated alkoxy groups.
  • the alkoxy groups can be substituted with groups such as alkenyl, alkynyl, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate
  • amine or “amino” includes compounds where a nitrogen atom is covalently bonded to at least one carbon or heteroatom.
  • alkyl amino includes groups and compounds wherein the nitrogen is bound to at least one additional alkyl group.
  • dialkyl amino includes groups wherein the nitrogen atom is bound to at least two additional alkyl groups.
  • arylamino and “diarylamino” include groups wherein the nitrogen is bound to at least one or two aryl groups, respectively.
  • alkylarylamino alkylarninoaryi” or “aryla inoalkyi” refers to an amino group that is bound to at least one alkyl group and at least one aryl group.
  • alkaminoalkyl refers to an alkyl, alkenyl, or alkynyl group bound to a nitrogen atom that is also bound to an alkyl group.
  • amide or “aminocarboxy” includes compounds or moieties that contain a nitrogen atom that is bound to the carbon of a carbonyl or a thiocarbonyl group.
  • alkaminocarboxy groups that include alkyl, alkenyl, or alkynyl groups bound to an amino group bound to a carboxy group. It includes arylaminocarboxy groups that include aryl or heteroaryl moieties bound to an amino group which is bound to the carbon of a carbonyl or thiocarbonyl group.
  • alkylaminocarboxy include moieties wherein alkyl, alkenyl, alkynyl and aryl moieties, respectively, are bound to a nitrogen atom which is in turn bound to the carbon of a carbonyl group.
  • carbonyl or “carboxy” includes compounds and moieties which contain a carbon connected with a double bond to an oxygen atom, and tautomeric forms thereof.
  • moieties that contain a carbonyl include aldehydes, ketones, carboxylic acids, amides, esters, anhydrides, etc.
  • carboxy moiety refers to groups such as “alkylcarbonyl” groups wherein an alkyl group is covalently bound to a carbonyl group, “alkenylcarbonyi” groups wherein an alkenyl group is covalently bound to a carbonyl group, "alkynylcarbonyl” groups wherein an alkynyl group is covalently bound to a carbonyl group, “arylcarbonyl” groups wherein an aryl group is covalently attached to the carbonyl group.
  • the term also refers to groups wherein one or more heteroatoms are covalently bonded to the carbonyl moiety.
  • the term includes moieties such as, for example, aminocarbonyl moieties, (wherein a nitrogen atom is bound to the carbon of the carbonyl group, e.g., an amide), aminocarbonyloxy moieties, wherein an oxygen and a nitrogen atom are both bond to the carbon of the carbonyl group (e.g., also referred to as a "carbamate").
  • aminocarbonylamino groups e.g., ureas
  • heteroatoms e.g. , nitrogen, oxygen, sulfur, etc. as well as carbon atoms.
  • heteroatom can be further substituted with one or more alkyl, alkenyl, alkynyl, aryl, aralkyl, acyl, etc. moieties.
  • thiocarbonyl or “thiocarboxy” includes compounds and moieties which contain a carbon connected with a double bond to a sulfur atom.
  • thiocarbonyl moiety includes moieties that are analogous to carbonyl moieties.
  • thiocarbonyl moieties include aminothiocarbonyl, wherein an amino group is bound to the carbon atom of the thiocarbonyl group, furthermore other thiocarbonyl moieties include, oxythiocarbonyls (oxygen bound to the carbon atom), aminothiocarbonylamino groups, etc.
  • ether includes compounds or moieties that contain an oxygen bonded to two different carbon atoms or heteroatoms.
  • alkoxyalkyl which refers to an alkyl, alkenyl, or alkynyl group covalently bonded to an oxygen atom that is covalently bonded to another alkyl group.
  • esters includes compounds and moieties that contain a carbon or a heteroatom bound to an oxygen atom that is bonded to the carbon of a carbonyl group.
  • ester includes alkoxycarboxy groups such as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, butoxycarbonyl, pentoxy carbonyl, etc.
  • alkyl, alkenyl, or alkynyl groups are as defined above.
  • thioether includes compounds and moieties which contain a sulfur atom bonded to two different carbon or hetero atoms.
  • Examples of thioethers include, but are not limited to alkthioalkyls, alkthioalkenyls, and alkthioalkynyls.
  • alkthioalkyls alkthioalkyls
  • -21- include compounds with an alkyl, alkenyl, or alkynyl group bonded to a sulfur atom that is bonded to an alkyl group.
  • alkthioalkenyls and alkthioalkynyls refer to compounds or moieties wherein an alkyl, alkenyl, or alkynyl group is bonded to a sulfur atom which is covalently bonded to an alkynyl group.
  • hydroxy or “hydroxyl” includes groups with an -OH or -O " .
  • halogen includes fluorine, bromine, chlorine, iodine, etc.
  • perhalogenated generally refers to a moiety wherein all hydrogens are replaced by halogen atoms.
  • polycyclyl or “polycyclic radical” include moieties with two or more rings (e.g., cycloalkyls, cycloalkenyls, cycloalkynyls, aryls and/or heterocyclyls) in which two or more carbons are common to two adjoining rings, e.g., the rings are "fused rings". Rings that are joined through non-adjacent atoms are termed "bridged" rings.
  • Each of the rings of the polycycle can be substituted with such substituents as described above, as for example, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkoxycarbonyl, alkylaminoacarbonyl, aralkylaminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, alkenylcarbonyl, aminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and urei
  • heteroatom includes atoms of any element other than carbon or hydrogen. Preferred heteroatoms are nitrogen, oxygen, sulfur and phosphorus.
  • heterocycle or “heterocyclic” includes saturated, unsaturated, aromatic (“heteroaryls” or “heteroaromatic”) and polycyclic rings which contain one or more heteroatoms.
  • heterocycles include, for example, benzodioxazole, benzofuran, benzoimidazole, benzothiazole, benzothiophene, benzoxazole, deazapurine, furan, indole, indolizine, imidazole, isooxazole, isoquinoline, isothiaozole, methylenedioxyphenyl, napthridine, oxazole, purine, pyrazine, pyrazole, pyridazine, pyridine, pyrimidine, pyrrole, quinoline, tetrazole, thiazole, thiophene, and triazole.
  • heterocycles include morpholine, piprazine, piperidine, thiomorpholine, and thioazolidine.
  • the heterocycles may be substituted or unsubstituted.
  • substituents include, for example, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkoxycarbonyl, alkylaminoacarbonyl, aralkylaminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, alkenylcarbonyl,
  • alkylthiocarbonyl alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkyl, alkylaryl, or an aromatic or heteroaromatic moiety.
  • the structures of some of the compounds of this invention include asymmetric carbon atoms. It is to be understood accordingly that the isomers arising from such asymmetry (e.g., all enantiomers and diastereomers) are included within the scope of this invention, unless indicated otherwise. Such isomers can be obtained in substantially pure form by classical separation techniques and by stereochemically controlled synthesis. Furthermore, the structures and other compounds and moieties discussed in this application also include all tautomers thereof. Compounds described herein may be obtained though art recognized synthesis strategies.
  • the Pinl modulating compound of formula (I) is any one of the compounds of Table 1, Table 2, Table 3, Table 4, Table 5, Table 6, or derivatives thereof.
  • the invention pertains to the Pinl -modulating compounds of formula (I), formula (II), or formula (III) described herein.
  • Particular embodiments of the invention pertain to the modulating compounds of Table 1, Table 2, Table 3, Table 4, Table 5, Table 6, or derivatives thereof.
  • the invention pertains to pharmaceutical compositions comprising the Pinl -modulating compounds described herein and a pharmaceutical acceptable carrier.
  • the another embodiment, the invention is intended to include any novel compound described herein.
  • the invention pertains, at least in part, to a method for treating cyclin Dl overexpression in a subject.
  • This method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I), formula (II), or formula (III), as described above, such that the cyclin Dl overexpression is treated.
  • the overexpression of cyclin Dl is associated with the presence of breast cancer in the subject.
  • cyclin Dl overexpression or “elevation in. the expression of cyclin Dl” includes cells having higher than normal levels of cyclin Dl.
  • Significant cyclin Dl overexpression includes both small and large increases in the levels of cyclin Dl compared with normal levels.
  • cyclin Dl overexpression is considered in the context of the phase of the cell cycle. In actively proliferating normal cells, cyclin Dl reaches a peak in mid Gi phase, decreases during S-phase, and remains low throughout the rest of the cycle, however, in transformed cells the level of cyclin Dl is more variable.
  • cyclin Dl overexpression includes the expression of cyclin Dl at levels that are abnormally high for the particular cell cycle phase of the cell. Cyclin Dl overexpression can manifest itself as tumor growth or cancer.
  • One skilled in the art would recognize the comparative studies that have been done measuring the level of cyclin Dl expression in normal cells in comparison with cells having a cancerous state. Increased cyclin D 1 expression has been found in a vast range of primary human tumors. Increased cyclin Dl expression has been detected in the form of gene amplification, increased cyclin Dl RNA expression, and increased cyclin Dl protein expression. Most clinical studies comparing cyclin Dl gene amplification with
  • cyclin Dl cyclin Dl RNA and/or protein expression without gene amplification suggests that other cellular genes such as pRb may affect the expression cyclin Dl .
  • Human tumors found to have increased cyclin Dl expression include: parathyroid adenomas, mantle cell lymphomas, breast cancers, head and neck squamous cell carcinomas (i.e.
  • squamous carcinomas in the oral cavity nasopharynx, pharynx, hypopharynx, and larynx
  • esophageal cancers hepatocellular carcinomas, colorectal cancers, genitourinary cancers
  • lung cancers i.e. squamous cell carcinomas of the lung
  • skins cancers i.e. squamous cell carcinomas, melanomas, and malignant fibrous histiocytomas
  • sarcomas i.e.
  • cyclin Dl The cyclin Dl gene is amplified in approximately 20% of mammary carcinomas and the protein is overexpressed in approximately 50% of mammary carcinomas (Barnes, et al. 1998, Breast Cancer Research and Treatment.
  • Cyclin Dl overexpression in mantle cell lymphoma is discussed in Espinet, et a ⁇ . 1999. Cancer Genet Cytogenet. lll(l):92-8 and Stamatopoulous, et al. 1999. Br. J. Haematol 105(1): 190-7. Cyclin Dl overexpression in breast cancer is discussed in Fredersdorf, et al. 1997. EN4S 94(12):6380-5. Cyclin Dl overexpression in head and neck cancers is discussed in Matthias, et al 1999. Cancer Epidemiol. Biomarkers Prev. 8(9):815-23; Matthias, et al. 1998. Clin. Cancer Res.
  • Cyclin Dl overexpression in laryngeal carcinoma is discussed in Bellacosa, et al. 1996. Clin. Cancer Res. 2(l):175-80. Cyclin Dl overexpression in multiple myeloma is discussed in Hoechtlen-Nollmar, et al. 2000. Br. J. Haematol 109(l):30-8; Pruneri, et al. 2000. Am. J. Pathol. 156(5): 1505-13; and
  • Cyclin Dl expression is regulated by many factors.
  • Growth factors i.e. CSF1, platelet-derived growth factor, insulin-like growth factor, steroid hormones, prolactin, and serum stimulation
  • CSF1 platelet-derived growth factor
  • insulin-like growth factor e.g., insulin-like growth factor
  • steroid hormones e.g., prolactin, and serum stimulation
  • CKIs cyclin dependent kinase inhibitors
  • CKIs High levels of CKIs bind to cdks and reduce the ability of cyclins to activate the cdks.
  • the two classes of CKIs are the Kip/Cip family including p21, p27, and p57, capable of binding to and inhibiting most cyclin-cdk complexes, and the I ⁇ K4 family including pl5, pl6, 18, and pl9, which seem to be specific inhibitors of cyclin Dl-cdk complexes.
  • Kip/Cip family including p21, p27, and p57
  • I ⁇ K4 family including pl5, pl6, 18, and pl9
  • CKI ⁇ l6 is activated by pRb and E2F, while the levels of CKI p27 are increased by TGF- ⁇ , cAMP, contact inhibition, and serum deprivation. Barnes, etal. 1998. Breast Cancer Research and Treatment. 52:1- 15. Cyclin Dl is believed to act through the phosphorylation of pRB, which is hypophosphorylated throughout the Gi phase, phosphorylated just before the S phase, and remains phosphorylated until late mitosis. Hypophosphorylated pRB arrests cells in Gi by forming a complex with the E2F family of DNA binding proteins that transcribe genes associated with DNA replication (the S phase of the cell cycle).
  • Cyclin D 1 can form a complex with either cdk4 or cdk6 to form activated cdk4 or cdk6.
  • Activated cdk4 or cdk6 induces the phosphorylation of pRb changing pRb from its hypophosphorylated form in which it binds to and inactivates E2F transcription factors to phosphorylated pRb that no longer binds to nor inactivates E2F transcription factors.
  • pRb is hyperphosphorylated compared with pRb in cells not overexpressing D c clins. It appears that cyclin Dl is required to initiate the phosphorylation of pRb that, in turn, drives the cell through the restriction point at which stage the cell is committed to divide.
  • Neoplasma or “neoplastic transformation” is the pathologic process that results in the formation and growth of a neoplasm, tissue mass, or tumor. Such process includes uncontrolled cell growth, including either benign or malignant tumors. Neoplasms include abnormal masses of tissue, the growth of which exceeds and is uncoordinated with that of the normal tissues and persists in the same excessive manner after cessation of the stimuli that evoked the change. Neoplasms may show a partial or complete lack of structural organization and functional coordination with the normal tissue, and usually form a distinct mass of tissue. One cause of neoplasia is dysregulation of the cell cycle machinery.
  • Neoplasms tend to grow and function somewhat independently of the homeostatic mechanisms that control normal tissue growth and function. However, some neoplasms remain under the control of the homeostatic mechanisms that control normal tissue growth and function. For example, some neoplasms are estrogen sensitive and can be arrested by anti-estrogen therapy. Neoplasms can range in size from less than 1 cm to over 6 inches in diameter. A neoplasm even 1 cm in diameter can cause biliary obstructions and jaundice, if it arises in and obstructs the ampulla of Nater. Neoplasms tend to morphologically and functionally resemble the tissue from which they originated.
  • neoplasms arising within the islet tissue of the pancreas resemble the islet tissue, contain secretory granules, and secrete insulin.
  • Clinical features of a neoplasm may result from the function of the tissue from which it
  • excessive amounts of insulin can be produced by islet cell neoplasms resulting in hypoglycemia which, in turn, results in headaches and dizziness.
  • some neoplasms show little morphological or functional resemblance to the tissue from which they originated.
  • Some neoplasms result in such non-specific systemic effects as cachexia, increased susceptibility to infection, and fever.
  • neoplasm By assessing the histology and other features of a neoplasm, it can be determined whether the neoplasm is benign or malignant. Invasion and metastasis (the spread of the neoplasm to distant sites) are definitive attributes of malignancy. Despite the fact that benign neoplasms may attain enormous size, they remain discrete and distinct from the adjacent non-neoplastic tissue. Benign tumors are generally well circumscribed and round, have a capsule, and have a grey or white color, and a uniform texture. In contrast, malignant tumors generally have fingerlike projections, irregular margins, are not circumscribed, and have a variable color and texture. Benign tumors grow by pushing on adjacent tissue as they grow. As the benign tumor enlarges it compresses adjacent tissue, sometimes causing atrophy. The junction between a benign tumor and surrounding tissue may be converted to a fibrous connective tissue capsule allowing for easy surgical removal of the benign tumor.
  • malignant tumors are locally invasive and grow into the adjacent tissues usually giving rise to irregular margins that are not encapsulated making it necessary to remove a wide margin of normal tissue for the surgical removal of malignant tumors.
  • Benign neoplasms tend to grow more slowly and tend to be less autonomous than malignant tumors.
  • Benign neoplasms tend to closely histologically resemble the tissue from which they originated.
  • More highly differentiated cancers i. e., cancers that resemble the tissue from which they originated, tend to have a better prognosis than poorly differentiated cancers, while malignant tumors are more likely than benign tumors to have an aberrant function, e.g., the secretion of abnormal or excessive quantities of hormones.
  • anaplasia Malignant neoplasms often contain numerous mitotic cells. These cells are typically abnormal. Such mitotic aberrations account for some of the karyotypic abnormalities found in most cancers. Bizarre multinucleated cells are also seen in some cancers, especially those that are highly anaplastic.
  • anaplasia includes histological features of cancer. These features include derangement of the normal tissue architecture, the crowding of cells, lack of cellular orientation termed dyspolarity, and cellular heterogeneity in size and shape termed “pleomorphism.”
  • the cytologic features of anaplasia include an increased nuclear-cytoplasmic ratio (nuclear-cytoplasmic ratio can be over 50% for malignant cells), nuclear pleomorphism, clumping of the nuclear chromatin along the nuclear
  • dysplasia includes pre-malignant states in which a tissue demonstrates histologic and cytologic features intermediate between normal and anaplastic. Dysplasia is often reversible.
  • cancer includes malignancies characterized by deregulated or uncontrolled cell growth, for instance carcinomas, sarcomas, leukemias, and lymphomas.
  • cancer includes primary malignant tumors, e.g. , those whose cells have not migrated to sites in the subject's body other than the site of the original tumor, and secondary malignant tumors, e.g., those arising from metastasis, the migration of tumor cells to secondary sites that are different from the site of the original tumor.
  • carcinoma includes malignancies of epithelial or endocrine tissues, including respiratory system carcinomas, gastrointestinal system carcinomas, genitourinary system carcinomas, testicular carcinomas, breast carcinomas, prostate carcinomas, endocrine system carcinomas, melanomas, choriocarcinoma, and carcinomas of the cervix, lung, head and neck, colon, and ovary.
  • carcinoma also includes carcinosarcomas, which include malignant tumors composed of carcinomatous and sarcomatous tissues.
  • adenocarcmoma includes carcinomas derived from glandular tissue or a tumor in which the tumor cells form recognizable glandular structures.
  • sarcoma includes malignant tumors of mesodermal connective tissue, e.g., tumors of bone, fat, and cartilage.
  • leukemia and “lymphoma” include malignancies of the hematopoietic cells of the bone marrow. Leukemias tend to proliferate as single cells, whereas lymphomas tend to proliferate as solid tumor masses. Examples of leukemias include acute myeloid leukemia (AML), acute promyelocytic leukemia, chronic myelogenous leukemia, mixed-lineage leukemia, acute monoblastic leukemia, acute lymphoblastic leukemia, acute non-lymphoblastic leukemia, blastic mantle cell leukemia, myelodyplastic syndrome, T cell leukemia, B cell leukemia, and chronic lymphocytic leukemia.
  • AML acute myeloid leukemia
  • AML acute promyelocytic leukemia
  • chronic myelogenous leukemia mixed-lineage leukemia
  • acute monoblastic leukemia acute lymphoblastic leukemia
  • acute non-lymphoblastic leukemia acute non-lympho
  • lymphomas examples include Hodgkin's disease, non- Hodgkin's lymphoma, B cell lymphoma, epitheliotropic lymphoma, composite lymphoma, anaplastic large cell lymphoma, gastric and non-gastric mucosa-associated lymphoid tissue lymphoma, lymphoproliferative disease, T cell lymphoma, Burkitt's lymphoma, mantle cell lymphoma, diffuse large cell lymphoma, Iymphoplasmacytoid lymphoma, and multiple myeloma.
  • the therapeutic methods of the present invention can be applied to cancerous cells of mesenchymal origin, such as those producing sarcomas (e.g., fibrosarcoma, myxosarcoma, liosarcoma, chondrosarcoma, osteogenic sarcoma or chordosarcoma, angiosarcoma, endotheliosardcoma, lympangiosarcoma, synoviosarcoma or mesothelisosarcoma); leukemias and lymphomas such as granulocytic leukemia, monocytic leukemia, lymphocytic leukemia, malignant lymphoma, plasmocytoma, reticulum cell sarcoma, or Hodgkin's disease; sarcomas such as leiomysarcoma or rhabdomysarcoma, tumors of epithelial origin such as squamous cell carcinoma, bas
  • Additional cell types amenable to treatment according to the methods described herein include those giving rise to mammary carcinomas, gastrointestinal carcinoma, such as c ⁇ lonic carcinomas, bladder carcinoma, prostate carcinoma, and squamous cell carcinoma of the neck and head region.
  • cancers amenable to treatment according to the methods described herein include vaginal, cervical, and breast cancers.
  • the language "inhibiting undesirable cell growth” is intended to include the inhibition of undesirable or inappropriate cell growth.
  • the inhibition is intended to include inhibition of proliferation including rapid proliferation.
  • the cell growth can result in benign masses or the inhibition of cell growth resulting in malignant tumors.
  • Examples of benign conditions which result from inappropriate cell growth or angiogenesis are diabetic retinopathy, retrolental fibrioplasia, neovascular glaucoma, psoriasis, angiofibromas, rheumatoid arthritis, hemangiomas, Karposi's sarcoma, and other conditions or dysfunctions characterized by dysregulated endothelial cell division.
  • the language "inhibiting tumor growth” or “inhibiting neoplasia” includes the prevention of the growth of a tumor in a subject or a reduction in the growth of a pre-existing tumor in a subject. The inhibition also can be the inhibition of the metastasis of a tumor from one site to another.
  • tumor is intended to encompass both in vitro and in vivo tumors that form in any organ or body part of the subject.
  • the tumors preferably are tumors sensitive to the Pinl -modulating compounds of the present invention.
  • Examples of the types of tumors intended to be encompassed by the present invention include those tumors associated with breast cancer, skin cancer, bone cancer, prostate cancer, liver cancer, lung cancer, brain cancer, cancer of the larynx, gallbladder, esophagus, pancreas, rectum, parathyroid, thyroid,
  • the tumors whose growth rate is inhibited by the present invention include basal cell carcinoma, squamous cell carcinoma of both ulcerating and papillary type, metastatic skin carcinoma, osteo sarcoma, Ewing's sarcoma, veticulum cell sarcoma, myeloma, giant cell tumor, small-cell lung tumor, gallstones, islet cell tumor, primary brain tumor, acute and chronic lymphocytic and granulocytic tumors, hairy-cell tumor, adenoma, hyperplasia, medullary carcinoma, pheochromocytoma, mucosal neuromas, intestinal ganglloneuromas, hyperplastic corneal nerve tumor, marfanoid habitus tumor, Wilm's tumor, seminoma, ovarian tumor, leiomyomater tumor, cervical dysplasia and in situ carcinoma, neuroblastoma, retinoblasto
  • the Pinl modulating compounds of the present invention may be used to treat, inhibit, and/or prevent undesirable cell growth, neoplasia, and/or cancer in any subject.
  • the Pinl modulating compounds of the present invention may be used to inhibit Pinl activity in a subject.
  • the Pinl modulating compounds of the present invention may be used to inhibit cyclin Dl expression in a subject.
  • the invention pertains, at least in part, to a method fortreating a Pinl-associated state in a subject.
  • the method includes administering to a subject an effective amount of a combination of a Pinl modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, and a hyperplastic inhibitory agent to treat the Pinl associated states.
  • a Pinl modulating compound of the invention e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above
  • a hyperplastic inhibitory agent to treat the Pinl associated states.
  • the invention pertains, at least in part, to a method for treating cyclin Dl overexpression in a subject.
  • the method includes administering to a subject an effective amount of a combination of a Pinl modulating compound of the invention, e.g. , Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, and a hyperplastic inhibitory agent to treat the cyclin Dl overexpression.
  • a Pinl modulating compound of the invention e.g. , Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above
  • a hyperplastic inhibitory agent to treat the cyclin Dl overexpression.
  • the invention pertains, at least in part, to a method for treating cancer in a subject.
  • the method includes administering to a subject an effective amount of a combination of a Pinl modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, and a hyperplastic inhibitory agent to treat the cancer.
  • a Pinl modulating compound of the invention e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above
  • a hyperplastic inhibitory agent e.g., as described above
  • hyperplastic inhibitory agent includes agents that inhibit the growth of proliferating cells or tissue wherein the growth of such cells or tissues is undesirable.
  • the inhibition can be of the growth of malignant cells, such as in neoplasms or benign cells, e.g., in tissues where the growth is inappropriate.
  • agents that can be used include chemotherapeutic agents, radiation therapy treatments, including therapeutically effective ranges of light, e.g. , laser light and/or immunofluorescent compounds, and associated radioactive compounds and methods, immunotoxins, and combinations thereof.
  • chemotherapeutic agent includes chemical reagents that inhibit the growth of proliferating cells or tissues wherein the growth of such cells or tissues is undesirable. Chemotherapeutic agents are well known in the art (see e.g., Gilman A.G., et al, The Pharmacological Basis of Therapeutics, 8th Ed., Sec 12:1202- 1263 (1990)), and are typically used to treat neoplastic diseases. The chemotherapeutic agents generally employed in chemotherapy treatments are listed below in Table 7.
  • chemotherapeutic agents include: bleomycin, docetaxel (Taxotere), doxorubicin, edatrexate, etoposide, finasteride (Proscar), flutamide (Eulexin), gemcitabine (Gemzar), goserelin acetate (Zoladex), granisetron (Kytril), irinotecan (Campto/Camptosar), ondansetron (Zofran), paclitaxel (Taxol), pegaspargase (Oncaspar), pilocarpine hydrochloride (Salagen), porfimer sodium (Photofrin), interleukin-2 (Proleukin), rituximab (Rituxan), topotecan (Hycamtin), trastuzumab (Herceptin), tretinoin (Retin-A), Triapine, vincristine, and vinorelbine tartrate (Navelbine
  • radiation therapy includes the application of a genetically and somatically safe level of electrons, protons, or photons, both localized and non- localized, to a subject to inhibit, reduce, or prevent symptoms or conditions associated with undesirable cell growth.
  • X-rays is also intended to include machine- generated radiation, clinically acceptable radioactive elements, and isotopes thereof, as well as the radioactive emissions therefrom. Examples of the types of emissions include alpha rays, beta rays including hard betas, high-energy electrons, and gamma rays.
  • Radiation therapy is well known in the art (see e.g. , Fishbach, F., Laboratory Diagnostic Tests, 3rd Ed., Ch. 10: 581-644 (1988)), and is typically used to treat neoplastic diseases.
  • immunotoxins includes immunotherapeutic agents that employ cytotoxic T cells and/or antibodies, e.g., monoclonal, polyclonal, phage antibodies, or fragments thereof, which are utilized in the selective destruction of undesirable rapidly proliferating cells.
  • immunotoxins can include antibody-toxin conjugates (e.g., Ab-ricin and Ab-diptheria toxin), antibody-radiolabels (e.g., Ab-ll3 ) ⁇ d antibody activation of the complement at the tumor cell.
  • antibody-toxin conjugates e.g., Ab-ricin and Ab-diptheria toxin
  • antibody-radiolabels e.g., Ab-ll3
  • the use of immunotoxins to inhibit, reduce, or prevent symptoms or conditions associated with neoplastic diseases are well known in the art (see, e.g., Harlow, E. and Lane, D., Antibodies, (1988)).
  • the invention includes a packaged Pinl-associated state treatment.
  • the packaged treatment includes a Pinl modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, packaged with instructions for using an effective amount of the
  • the invention includes a packaged cyclin Dl overexpression treatment.
  • This packaged treatment include a Pinl modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, packaged with instructions for using an effective amount of the Pinl modulating compound to treat cyclin Dl overexpression.
  • the invention also pertains, at least in part to a packaged cancer treatment, which includes a Pinl -modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, packaged with instructions for using an effective amount of the Pinl -modulating compound to treat cancer.
  • a Pinl -modulating compound of the invention e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, packaged with instructions for using an effective amount of the Pinl -modulating compound to treat cancer.
  • the invention also pertains, at least in part, to pharmaceutical compositions of comprising Pinl -modulating compounds of the invention, e.g., Pinl- modulating compounds of formula (I), formula (II), or formula (III), as described above, and, optionally, a pharmaceutically acceptable carrier.
  • Pinl -modulating compounds of the invention e.g., Pinl- modulating compounds of formula (I), formula (II), or formula (III), as described above, and, optionally, a pharmaceutically acceptable carrier.
  • an effective amount of the compound is that amount necessary or sufficient to treat or prevent a Pinl associated state, e.g. prevent the various morphological and somatic symptoms of a Pinl associated state.
  • an effective amount of the Pinl -modulating compound is the amount sufficient to inhibit undesirable cell growth in a subject.
  • an effective amount of the Pinl -modulating compound is the amount sufficient to inhibit undesirable cell growth in a subject.
  • Pinl -modulating compound is the amount sufficient to reduce the size of a pre-existing benign cell mass or malignant tumor in a subject.
  • the effective amount can vary depending on such factors as the size and weight of the subject, the type of illness, or the particular Pinl binding compound. For example, the choice of the Pinl binding compound can affect what constitutes an "effective amount".
  • One of ordinary skill in the art would be able to study the factors contained herein and make the determination regarding the effective amount of the Pinl binding compound without undue experimentation.
  • an effective amount of a Pinl -modulating compound can be determined by assaying for the expression of cyclin Dl and determining the amount of the Pinl -modulating compound sufficient to reduce the levels of cyclin Dl to that associated with a non-cancerous state.
  • the regimen of administration can affect what constitutes an effective amount.
  • the Pinl binding compound can be administered to the subject either prior to or after the onset of a Pinl associated state. Further, several divided dosages, as well as staggered dosages, can be administered daily or sequentially, or the dose can be continuously infused, or can be a bolus injection. Further, the dosages of the Pinl binding compound(s) can be proportionally increased or decreased as indicated by the exigencies of the therapeutic or prophylactic situation.
  • composition includes preparations suitable for administration to mammals, e.g., humans.
  • pharmaceutical composition containing, for example, 0.1 to 99.5% (more preferably, 0.5 to 90%) of active ingredient in combination with a pharmaceutically acceptable carrier.
  • phrases "pharmaceutically acceptable carrier” is art recognized and includes a pharmaceutically acceptable material, composition or vehicle, suitable for administering compounds of the present invention to mammals.
  • the carriers include liquid or solid filler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting the subject agent from one organ, or portion of the body, to another organ, or portion of the body.
  • Each carrier must be “acceptable” in the sense of being compatible with the other ingredients of the formulation and not injurious to the patient.
  • materials which can serve as pharmaceutically acceptable carriers include: sugars, such as lactose, glucose and sucrose; starches, such as corn starch and potato starch; cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar; buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer'
  • wetting agents such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, release agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the compositions.
  • antioxidants examples include: water soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulf ⁇ te, sodium sulfite and the like; oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecithin, propyl gallate, ⁇ -tocopherol, and the like; and metal chelating agents, such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like.
  • water soluble antioxidants such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulf ⁇ te, sodium sulfite and the like
  • oil-soluble antioxidants such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT),
  • Formulations of the present invention include those suitable for oral, nasal, topical, transdermal, buccal, sublingual, rectal, vaginal and/or parenteral administration.
  • the formulations may conveniently be presented in unit dosage form and
  • the amount of active ingredient that can be combined with a carrier material to produce a single dosage form will generally be that amount of the compound that produces a therapeutic effect. Generally, out of one hundred per cent, this amount will range from about 1 per cent to about ninety-nine percent of active ingredient, preferably from about 5 per cent to about 70 per cent, most preferably from about 10 per cent to about 30 per cent.
  • Methods of preparing these formulations or compositions include the step of bringing into association a compound of the present invention with the carrier and, optionally, one or more accessory ingredients.
  • the formulations are prepared by uniformly and intimately bringing into association a compound of the present invention with liquid carriers, or finely divided solid carriers, or both, and then, if necessary, shaping the product.
  • Formulations of the invention suitable for oral administration may be in the form of capsules, cachets, pills, tablets, lozenges (using a flavored basis, usually sucrose and acacia or tragacanth), powders, granules, or as a solution or a suspension in an aqueous or non-aqueous liquid, or as an oil-in- water or water-in-oil liquid emulsion, or as an elixir or syrup, or as pastilles (using an inert base, such as gelatin and glycerin, or sucrose and acacia) and/or as mouth washes and the like, each containing a predetermined amount of a compound of the present invention as an active ingredient.
  • a compound of the present invention may also be administered as a bolus, electuary or paste.
  • the active ingredient is mixed with one or more pharmaceutically acceptable carriers, such as sodium citrate or dicalcium phosphate, and/or any of the following: fillers or extenders, such as starches, lactose, sucrose, glucose, mannitol, and/or silicic acid; binders, such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinyl pyrrolidone, sucrose and/or acacia; humectants, such as glycerol; disintegrating agents, such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate; solution retarding agents, such as paraffin; absorption accelerators, such as quaternary ammonium compounds; wetting agents, such as, for example, cetyl alcohol and glycerol monostea
  • compositions may also comprise buffering agents.
  • Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugars, as well as high molecular weight polyethylene glycols and the like.
  • a tablet may be made by compression or molding, optionally with one or more accessory ingredients.
  • Compressed tablets may be prepared using binder (for example, gelatin or hydroxypropylmethyl cellulose), lubricant, inert diluent, preservative, disintegrant (for example, sodium starch glycolate or cross-linked sodium carboxymethyl cellulose), surface-active or dispersing agent.
  • Molded tablets may be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.
  • the tablets, and other solid dosage forms of the pharmaceutical compositions of the present invention may optionally be scored or prepared with coatings and shells, such as enteric coatings and other coatings well known in the pharmaceutical-formulating art. They may also be formulated so as to provide slow or controlled release of the active ingredient therein using, for example, hydroxypropylmethyl cellulose in varying proportions to provide the desired release profile, other polymer matrices, liposomes and/or microspheres.
  • compositions may be sterilized by, for example, filtration through a bacteria-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions that can be dissolved in sterile water, or some other sterile injectable medium immediately before use.
  • These compositions may also optionally contain opacifying agents and may be of a composition that they release the active ingredient(s) only, or preferentially, in a certain portion of the gastrointestinal tract, optionally, in a delayed manner.
  • embedding compositions that can be used include polymeric substances and waxes.
  • the active ingredient can also be in micro-encapsulated form, if appropriate, with one or more of the above-described excipients.
  • Liquid dosage forms for oral administration of the compounds of the invention include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
  • the liquid dosage forms may contain inert diluent commonly used in the art, such as, for example, water or other solvents, solubilizing agents and mulsifiers, such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (in particular, cottonseed, groundnut, com, germ, olive, castor and sesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
  • inert diluent commonly used in the art, such as, for example, water or other solvents, solubilizing agents and mul
  • the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, coloring, perfuming and preservative agents.
  • adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, coloring, perfuming and preservative agents.
  • Suspensions in addition to the active compounds, may contain suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide,
  • Formulations of the pharmaceutical compositions of the invention for rectal or vaginal administration may be presented as a suppository, which may be prepared by mixing one or more compounds of the invention with one or more suitable nonirritating excipients or carriers comprising, for example, cocoa butter, polyethylene glycol, a suppository wax or a salicylate, and which is solid at room temperature, but liquid at body temperature and, therefore, will melt in the rectum or vaginal cavity and release the active compound.
  • suitable nonirritating excipients or carriers comprising, for example, cocoa butter, polyethylene glycol, a suppository wax or a salicylate, and which is solid at room temperature, but liquid at body temperature and, therefore, will melt in the rectum or vaginal cavity and release the active compound.
  • Formulations of the present invention which are suitable for vaginal . administration also include pessaries, tampons, creams, gels, pastes, foams or spray formulations containing such carriers as are known in the art to be appropriate.
  • Dosage forms for the topical or transdermal administration of a compound of this invention include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches and inhalants.
  • the active compound may be mixed under sterile conditions with a pharmaceutically acceptable carrier, and with any preservatives, buffers, or propellants that may be required.
  • the ointments, pastes, creams and gels may contain, in addition to an active compound of this invention, excipients, such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof.
  • excipients such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof.
  • Powders and sprays can contain, in addition to a compound of this invention, excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and poly amide powder, or mixtures of these substances.
  • Sprays can additionally contain customary propellants, such as chlorofluorohydrocarbons and volatile unsubstituted hydrocarbons, such as butane and propane.
  • Transdermal patches have the added advantage of providing controlled delivery of a compound of the present invention to the body.
  • dosage forms can be made by dissolving or dispersing the compound in the proper medium.
  • Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate of such flux can be controlled by either providing a rate controlling membrane or dispersing the active compound in a polymer matrix or gel.
  • Ophthalmic formulations are also contemplated as being within the scope of this invention.
  • compositions of this invention suitable for parenteral administration comprise one or more compounds of the invention in combination with one or more pharmaceutically acceptable sterile isotonic aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, or sterile powders which may,be reconstituted into sterile injectable solutions or dispersions just prior to use, which may
  • -103- contain antioxidants, buffers, bacteriostats, solutes which render the formulation isotonic with the blood of the intended recipient or suspending or thickening agents.
  • aqueous and nonaqueous carriers examples include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), and suitable mixtures thereof, vegetable oils, such as olive oil, and injectable organic esters, such as ethyl oleate.
  • polyols such as glycerol, propylene glycol, polyethylene glycol, and the like
  • vegetable oils such as olive oil
  • injectable organic esters such as ethyl oleate.
  • Proper fluidity can be maintained, for example, by the use of coating materials, such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.
  • These compositions may also contain adjuvants such as preservatives, wetting agents, emulsifying agents and dispersing agents.
  • microorganisms Prevention of the action of microorganisms may be ensured by the inclusion of various antibacterial and antifungal agents, for example, paraben, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents that delay absorption such as aluminum monostearate and gelatin.
  • antibacterial and antifungal agents for example, paraben, chlorobutanol, phenol sorbic acid, and the like.
  • isotonic agents such as sugars, sodium chloride, and the like into the compositions.
  • prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents that delay absorption such as aluminum monostearate and gelatin.
  • Injectable depot forms are made by forming microencapsule matrices of the subject compounds in biodegradable polymers such as polylactide-polyglycolide.
  • the rate of drug release can be controlled.
  • biodegradable polymers include poly(orthoesters) and poly (anhydrides).
  • Depot injectable formulations are also prepared by entrapping the drug in liposomes or microemulsions that are compatible with body tissue.
  • the preparations of the present invention may be given orally, parenterally, topically, or rectally. They are of course given by forms suitable for each administration route. For example, they are administered in tablets or capsule form, by injection, inhalation, eye lotion, ointment, suppository, etc. administration by injection, infusion or inhalation; topical by lotion or ointment; and rectal by suppositories. Oral administration is preferred.
  • parenteral administration and “administered parenterally” as used herein means modes of administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal and intrasternal injection and infusion.
  • systemic administration means the administration of a compound, drug or other material other than directly into the central nervous system, such that it enters the patient's system and, thus, is subject to metabolism and other like processes, for example, subcutaneous administration.
  • These compounds may be administered to humans and other animals for therapy by any suitable route of administration, including orally, nasally, as by, for example, a spray, rectally, intravaginally, parenterally, intracistemally and topically, as by powders, ointments or drops, including buccally and sublingually.
  • the compounds of the present invention which may be used in a suitable hydrated form, and/or the pharmaceutical compositions of the present invention, are formulated into ⁇ pharmaceutically acceptable dosage forms by conventional methods known to those of skill in the art.
  • Actual dosage levels of the active ingredients in the pharmaceutical compositions of this invention may be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient.
  • the selected dosage level will depend upon a variety of factors including the activity of the particular compound of the present invention employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compound employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the medical arts.
  • a physician or veterinarian having ordinary skill in the art can readily determine and prescribe the effective amount of the pharmaceutical composition required.
  • the physician or veterinarian could start doses of the compounds of the invention employed in the pharmaceutical composition at levels lower than that required in order to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved.
  • a suitable daily dose of a compound of the invention will be that amount of the compound that is the lowest dose effective to produce a therapeutic effect. Such an effective dose will generally depend upon the factors described above.
  • intravenous and subcutaneous doses of the compounds of this invention for a patient when used for the indicated analgesic effects, will range from about 0.0001 to about 100 mg per kilogram of body weight per day, more preferably from about 0.01 to about 50 mg per kg per day, and still more preferably from about 1.0 to about 100 mg per kg per day.
  • An effective amount is that amount treats an Pinl associated state.
  • the effective daily dose of the active compound may be administered as two, three, four, five, six or more sub-doses administered separately at appropriate intervals throughout the day, optionally, in unit dosage forms.
  • a compound of the present invention While it is possible for a compound of the present invention to be administered alone, it is preferable to administer the compound as a pharmaceutical composition.
  • the invention is further illustrated by the following examples, which should not be construed as further limiting.
  • the animal models used throughout the Examples are accepted animal models and the demonstration of efficacy in these animal models is predictive of efficacy in humans.
  • Pinl -modulating compounds are potent antitumor agents.
  • the anti-tumor activity of Pinl -modulating compounds against glioblastoma cells is comparable to 1,3- bis(2-chloroethyl)-l -nitrosourea (BCNU), one of the most potent clinical useful antitumor agents. Misra, et al. 1982. J. Am. Chem. Soc. 104: 4478-4479
  • In vitro anti-tumor activity of Pinl -modulating compounds can be assayed by measuring the ability of Pinl -modulating compounds to kill tumor cells.
  • appropriate cells lines include: human lung (A549); resistant human lung with low topo II activity (A549-VP); murine melanoma (B 16); human colon tumor (HCT116); human colon tumor with elevated pl70 levels (HCTVM); human colon tumor with low topo II activity (HCTVP); P388 murine lymph leukemia cells; and human colon carcinoma cell line (Moser) under standard conditions. After the cells are cultured for twenty-four hours and allowed to attach to a plate (t ' .e.
  • -106- In vivo anti-tumor activity of Pinl-modulating compounds can be assayed for by a reduction of tumor cells in mammals (i.e. mice) and a resulting increase in survival time compared to untreated tumor bearing mammals.
  • a reduction of tumor cells in mammals i.e. mice
  • CDFi mice are injected interperitoneally with a suspension of P388 murine lymph leukemia cells, Ehrlich carcinoma cells, B 16 melanoma cells, or Meth-A fibrosarcoma cells or other appropriate tumor cell line. Some of the mice are treated intraperitoneally with a Pinl- modulating compounds. Other mice are treated with saline.
  • the in vivo activity of the compound is determined in terms of the % T/C which is the ratio of the mean survival time of the treated group to the mean survival time of the saline treated group times 100.
  • % T/C is the ratio of the mean survival time of the treated group to the mean survival time of the saline treated group times 100.
  • Pinl-modulating compounds can also be assayed as inhibitors against an ovarian tumor growing in a human tumor cloning system. Tebbe, et al. 1971 J. Am. Chem. Soc. 93:3793-3795.
  • Mammalian cells were seeded in 96 well flat bottom microtiter plates at a density of 5,000 6000 cells per well on day 0 in 0.1 mL of an appropriate growth media. On Day 1, the wells were aspirated and 0.1 mL of fresh media was added. The cells were then treated with 0.01 mL of 1 Ox drug dilutions in 10% DMSO in media and incubated at 37° C in a humidified, 5% CO atmosphere. The assay contained eight drug concentrations in triplicate as well as a triplicate control where cells were treated with 0.01 mL of 10% DMSO in media.
  • a colorimetric cell-viability assay solution prepared from 20 parts (3-(4,5- dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (Promega) at 2.0 mg/mL in PBS and 1 part phenazine methosulfate (Sigma) at 0.92 mg/mL in PBS for 2-3 hours at 37 °C.
  • Background wells were prepared by incubating 0.02 mL of the colorimetric cell-viability assay solution with 0.1 mL of media in parallel with the cell containing wells.
  • the absorbance at 490 nm was then measured with an ELISA plate reader and the absorbance recorded for the background wells was averaged and the mean value was subtracted from the cell containing wells. Percent cell viabilities at each drug concentration were calculated by dividing the mean absorbance at 490 nm of the treated wells by the mean absorbance at 490 nm of the untreated wells.
  • the proline isomerase activity assay is based on the method described by Fisher et al (Biomed. Biochim. Acta, 1984, 43: 1101-1111). Specifically, the enzyme (112 ng) was preincubated with 72 mM substrate at 4 °C for 30 minutes in an 80 ⁇ L reaction volume containing 0.02 mg/ ⁇ L BSA, 0.8 mM DTT, and 35 mM HEPES (pH 7.8).
  • Inhibition studies were preformed by adding 5 ⁇ L of inhibitors added in the pre-incubation mix. Inhibitors were at 0.4 mg/mL in 10% DMSO.
  • the specificity of the Pinl inhibitor compounds of the invention can be determined by the protease-coupled PPIase assay developed by Fischer et al. (Biomed. Biochim. Acta, 1984, 43: 1101-1111).
  • the enzyme activity of Pinl can be compared to members of the other known classes of PPIases, cyclophilins (e.g., hCypl ⁇ , hCyP-A, hCyP-B, hCyP-C, and NKCA) and FKBPs (e.g., hFKBP12, hFKBP-12, hFKBP-13, and hFKBP-25) in the presence and absence of the compound.
  • cyclophilins e.g., hCypl ⁇ , hCyP-A, hCyP-B, hCyP-C, and NKCA
  • FKBPs e.g., hFKBP12, hFKBP-12
  • hPinl activity measurements are determined using bovine trypsin (final concentration 0.21 mg/mL, Sigma) as an isomer specific protease and
  • the total reaction volume is adjusted to 1.23 mL by mixing appropriate volumes of 35 mM HEPES (pH 7.8) with enzyme and effector solutions.
  • the Pinl inhibitor compound is freshly diluted from a 1 mg/mL stock solution in DMSO, and pre-incubated at varying concentrations with the enzyme for 5 min (10°C). Prior to the start of reaction by addition of the respective protease, 2 ⁇ L of the peptide substrate stock solution (10 mg/mL in DMSO) is added. The amount of organic solvent is kept constant within each experiment ( ⁇ 0.1%).
  • the pseudo-first-order rate constant k 0bs for cis/trans isomerization in the presence of PPIase and the first-order rate constant k 0 of the uncatalyzed cis/trans isomerization can be calculated using the Kinetics Software of Hewlett-Packard as well as SigmaPlot2000 for Windows 6.0 (SPSS).
  • the K; value for inhibition of Pinl PPIase activity by a Pinl inhibitor compound of the invention at constant concentrations of substrate [S 0 ] «K M ) can then be calculated by fitting the data according to the equation for a competitive
  • a cell solution is added to a flask containing containing 13 ml of 10% FBS with EDTA.
  • the cell suspension is centrifuged at 1500g for 5 minutes and resuspend in 10 mL media. The centriguge procedure is repeated.
  • the cells are resuspended in 2 mL of media.
  • 20 ⁇ l of cell suspension is added to 180 ⁇ L 0.2% trypahn blue.
  • Approximately 2000 cells are added to each well of a micortitre plate in 100 ⁇ L media. After cells have grown for an appropriate time ( ⁇ l-2 days depending on cell line) 10 ⁇ L of a stock solution containing a test compound is added to each well. After further growth, the media is removed from the well and tyrpsin is added. After a short incubation, the trypsin is removed or inactivated and the cells are counted using a Guava Cell Analysis System (Hayward, CA).
  • the automated cellular imaging system was used to determine tissues with elevated Pinl Levels.
  • the data that is presented in Example 4 is from U.S. Patent Application No. 10/071,747, filed February 8, 2002, the entire contents of which are incorporated by reference.
  • Micro-histoarray sections were scanned and images were captured using the automated cellular imaging system (ChromaNision Medical Systems, Inc., San Juan Capistrano, CA), which combines automated microscopy and computerized image processing to analyze multiple tissues on a single slide.
  • ACIS was used to analyze microarray tissue sections on glass slides stained using a diaminodenzidine chromagen (DAB) and hematoxylin counterstain. Positive staining (brown color) as viewed by light microscope indicates the presence of the protein, and color intensity correlates directly with protein quantity (expression).
  • DAB diaminodenzidine chromagen
  • Positive staining brown color
  • the ACIS was able to recognize 255 levels of immnohistochemical staining intensity (0-255) and converted these to fractional scores for the selected individual areas.
  • the base limit on the threshold for the Generic DAB is pre-set at 50 by the manufacturer because the system is very sensitive. Therefore, any intensity below 50 was treated as 0 in this study. Entire immunostahied tissue sections were scanned using the 4 X objective and images were captured using the 10X objective.
  • Mass spectrometric data was obtained using a HP 6980 Gas Chromatograph coupled to a 5973 Mass Selective Detector: Agilent HP1 column, 15 m column length, 0.25 mm column diameter, 0.1 ⁇ m column film, 280°C injector temperature, initial oven temperature of 200°C for 3 minutes, changed to 325°C over 5 minutes, and held constant for 6 minutes. Thin-layer chromatography was performed on EM Science MK6F silica gel glass TLC plates and UN light was used for detecting compounds on the TLC plates.
  • Reagents used in reactions were purchased from Aldrich Chemical Company (Milwaukee, MO), Sigma Chemical Company (Milwaukee, MO), Fluka Chemical Company (Milwaukee, MO), Fisher Scientific (Pittsburg, PA), TCI America (Portland, OR), Ryan Scientific (Isle of Palms, SC), Lancaster Synthesis (Windham, ⁇ H ), Asinex (Moscow, Russia), Chembridge Corporation (San Diego, CA), Matrix Scientific (Columbia, SC) or Oakwood Products Inc. (West Columbia, SC).
  • Method A condenses an aldehyde with the active methylene of an appropriately substituted rhodanine carboxylic acid under Aldol conditions.
  • Method B condenses the aldehydes with the corresponding ester-protected carboxylic acid, followed by hydrolytic cleavage of the ester to provide the carboxylic acid.
  • Method C demonstrates that the Aldol condensation can be carried out using polymer-bound rhodanine. The final product can subsequently be released from the polymer support under mild conditions to provide the analogs of the invention.
  • the crude product was dissolved in ⁇ 500 mL of acetone and warmed on a hot plate, filtered warm and washed with warm acetone. The clear yellow/orange solution was placed back on a hot plate and brought to a gentle reflux. After - 50 mL of acetone had evaporated, - 50 mL of water was added and the solution allowed to continue gentle refluxing until an additional - 50 mL of acetone had evaporated. An additional 50 mL of water was added and the solution was allowed to continue refluxing until it became cloudy, at which point is was removed from the hot plate, washed down the sides with acetone, allowed to cool to room temperature, and then placed overnight in a refrigerator.
  • R2 5-(4-nitrophenyl)furanyi-

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Abstract

The invention is directed to modulators, e.g., inhibitors, of Pin 1 and Pin 1-related proteins and the use of such modulators for treatment of Pin 1 associated states, e.g., for the treatment of cancer.

Description

PINl-MODϋLATING COMPOUNDS AND METHODS OF USE THEREOF
Related Applications This application claims priority to U.S. Provisional Application No.
60/414,077 filed September 26, 2002, entitled "Pinl -Modulating Compounds and Methods of Use Thereof.
This application is related to U.S. Provisional Application No. 60/361,206 filed March 1, 2002, entitled "Pinl -Modulating Compounds and Methods of Use Thereof; U.S. Provisional Application Serial No. 60/361,246, filed March 1, 2002, entitled "Pinl- Modulating Compounds and Methods of Use Thereof; U.S. Provisional Application Serial No: 60/361,231, filed March 1, 2002, entitled "Pinl -Modulating Compounds and Methods of Use Thereof; U.S. Provisional Application Serial No. 60/361,227, filed on March 1, 2002; entitled "Methods for Designing Specific Inhibitors for Pinl Proline Isomerase and Pinl- Related Molecules"; U.S. Provisional Application No. 60/360,799 filed March 1 , 2002, entitled "Methods of Treating Pinl Associated Disorders"; PTZ-034-2, entitled "Pinl- Modulating Compounds and Methods of Use Thereof, filed March 3, 2003; PTZ-034, entitled "Pinl -Modulating Compounds and Methods of Use Thereof, filed March 3, 2003; PTZ-035-2, , entitled "Pinl -Modulating Compounds and Methods of Use Thereof," filed March 3, 2003; PTZ-035, entitled "Pinl -Modulating Compounds and Methods of Use Thereof," filed March 3, 2003; PTZ-036-2, entitled "Pinl -Modulating Compounds and Methods of Use Thereof, filed March 3, 2003; PTZ-036, entitled "Pinl -Modulating Compounds and Methods of Use Thereof, filed March 3, 2003; PTZ-037, entitled "Methods of Treating Pinl Associated Disorders," filed March 3, 2003; PTZ-009, entitled "Methods for Designing Specific Inhibitors for Pinl Proline Isomerase and Pinl -Related Molecules," filed March 3, 2003; and PTZ-046-2, entitled "Pinl -Modulating Compounds and Methods of Use Thereof, filed March 3, 2003. The entire contents of each of the aforementioned applications are hereby expressly incorporated herein by reference in their entireties.
Background of the Invention
The peptidyl-prolyl cis-trans isomerases (PPIases), or rotamases, are a family of ubiquitous enzymes that catalyze the cis/trans isomerization of the peptide bond on the N- terminal side of proline residues in proteins (Hunter, Cell 92:141-142, 1998). PPIases are divided into three classes, cyclophilins (Cyps), FK-506 binding proteins (FKBPs) and the Pinl/parvulin class. Cyclophilins and FKBPs are distinguished by their ability to bind the clinically immunosuppressive drugs cyclosporin and FK506, respectively (Schreiber, Science 251:283-7, 1991; Hunter, supra). Upon binding of these drugs, there are two common outcomes: inhibition of the PPIase activity and inhibition of the common target calcineurin. The inhibition of calcineurin phosphatase activity prevents lymphocytes from responding to antigen-induced mitogenic signals, thus resulting in immunusuppression. However, the inhibition of the PPIase activity is apparently unrelated to the immunosuppressive property of the drug/PPIase complexes. Even more surprisingly, deletion of all 8 known cyclophilins and 4 FKBPs in the same cells does not result in any significant phenotype (Dolinski et al., Proc. Natl. Acad. Sci. USA 94:13093-131098, 1997).
In contrast, members of the Pinl/parvulin class of PPIases bind neither of these immunosuppressive drugs, and are structurally unrelated to the other two classes of PPIases. Known members of the Pinl/parvulin class include Pinsl-3 (Lu et al., Nature 380;544-547, 1996), Pin-L (Campbell et al., Genomics 44:157-162, 1997), parvulin (Rahfeld, et al., Proc. Natl. Acad. Sci. USA 93:447-451, 1996) and Essl/Pftl (Hanes et al., Yeast 5:55- 72, 1989; and Hani, et al. FEBS Letts 365:198-202, 1995).
Pinl is a highly conserved protein that catalyzes the isomerization of only phosphorylated Ser/Thr-Pro bonds (Rananathan, R. et al. (1997) Cell 89:875-86; Yaffe, et al. 1997, Science 278:1957-1960; Shen, et al. 1998,Genes Dev. 12:706-720; Lu, et al. 1999, Science 283:1325-1328; Crenshaw, et al. 1998, Embo J. 17:1315-1327; Lu, et al. 1999, Nature 399:784-788; Zhou, et al. 1999, Cell Mol. Life Sci. 56:788-806). In addition, Pinl contains an N-terminal WW domain, which functions as a phosphorylated Ser/Thre-Pro binding module (Sudol, M. (1996) Prog. Biophys. Mol. Biol. 65:113-32). This phosphorylation-dependent interaction targets Pinl to a subset of phosphorylated substrates, including Cdc25, Wee 1, Mytl, Tau-Rad4, and the C-terminal domain of RNA polymerase II large domain (Crenshaw, D.G., et al (1998) Embo. J. 17:1315-27; Shen, M. (1998) Genes Dev. 12:706-20; Wells, NJ. (1999) J. Cell. Sci. 112: 3861-71).
The specificity of Pinl activity is essential for cell growth; depletion or mutations of Pinl cause growth arrest, affect cell cycle checkpoints and induce premature mitotic entry, mitotic arrest and apoptosis in human tumor cells, yeast or Xenopus extracts (Lu, et al. 1996, Nature 380:544-547; Winkler, et al. 200, Science 287:1644-1647; Hani, et al. 1999. J. Biol. Chem. 274:108-116). In addition, Pinl is dramatically overexpressed in human cancer samples and the levels of Pinl are correlated with the aggressiveness of tumors. Moreover, inhibition of Pinl by various approaches, including Pinl antisense polynucleotides or genetic depletion, kills human and yeast dividing cells by inducing premature mitotic entry and apoptosis. Thus, Pinl -catalyzed prolyl isomerization regulates the conformation and function of these phosphoprotein substrates and facilitates dephosphorylation because of the conformational specificity of some phosphatases. Thus, Pinl -dependent peptide bond isomerization is a critical post-phosphorylation regulatory mechanism, allowing cells to turn phosphoprotein function on or off with high efficiency and specificity during temporally regulated events, including the cell cycle (Lu et al, supra).
Summary of the Invention
A need exists for new diagnostic and therapeutic compounds for diseases characterized by uncontrolled cell proliferation and primarily malignancies associated with the Pin-1 subfamily of enzymes.
Accordingly, the invention is directed to modulators, e.g., inhibitors, of Pinl and Pinl -related proteins and the use of such modulators for treatment of Pinl associated states, e.g., for the treatment of cancer. In one embodiment, the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I):
Figure imgf000004_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1;
Z and Zi are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2O-, -S-, or -OCH2-, phosphoprotein function on or off with high efficiency and specificity during temporally regulated events, including the cell cycle (Lu et al, supra).
Summary of the Invention A need exists for new diagnostic and therapeutic compounds for diseases characterized by uncontrolled cell proliferation and primarily malignancies associated with the Pin-1 subfamily of enzymes.
Accordingly, the invention is directed to modulators, e.g., inhibitors, of Pinl and Pinl-related proteins and the use of such modulators for treatment of Pinl associated states, e. g. , for the treatment of cancer. In one embodiment, the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I):
Figure imgf000005_0001
(I)
wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is 0 or 1 ;
Z and Zi are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2O-, -S-, or -OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and - SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof; ■
Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; such that the Pinl -associated state is treated.
In a second embodiment, the invention pertains, at least in part, to a method for treating cyclin Dl overexpression in a subject. This method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I):
Figure imgf000006_0001
(0 wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 rough 10; m is O or 1;
Z and Zi are independently selected from the group consisting of O or
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2O-, -S-, or -OCH2 L, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and - SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; packaged with instructions for using an effective amount of the Pinl- modulating compound to treat a Pinl associated state.
The invention also includes a packaged cyclin Dl overexpression treatment. This packaged treatment include a Pinl -modulating compound of formula (I):
Figure imgf000007_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1;
Z and Zi are independently selected from the group consisting of O or
S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2O-, -S-, or -OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -
-5- SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
R1 is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; packaged with instructions for using an effective amount of the Pinl -modulating compound to treat cancer.
In another embodiment, the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to a subject an effective amount of a combination of a Pinl -modulating compound of formula (I):
Figure imgf000008_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1;
Z and Zi are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ-, -S-, or -OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -
-6- SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
Ri is selected from the group consisting of H; - X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein 2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; and a hyperplastic inhibitory agent such that the Pinl associated state is treated. In another embodiment, the invention pertains, at least in part, to a method for treating cancer in a subject. The method includes administering to the subject an effective amount of a combination of a Pinl -modulating compound of formula (I):
Figure imgf000009_0001
ω wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or l;
Z and Zi are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ-, -S-, or -OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and - SO2CF3, OAc, -O-iBu, -S(O) NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an
-7- integer ranging from about 0 to 4, and any combination thereof;
Rt is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; and a hyperplastic inhibitory agent such that the cancer is treated.
In an additional embodiment, the invention is a method for treating cyclin Dl overexpression in a subject. The method includes administering to the subject an effective amount of a combination of a Pinl -modulating compound of formula (I):
Figure imgf000010_0001
Φ wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is 0 or 1 ;
Z and Zi are independently selected from the group consisting of O or
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ-, -S-, or -OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -
SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
-8- Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 isN-thiazol-2-yl-benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide.
1 Another embodiment of the invention is a pharmaceutical composition comprising a Pinl -modulating compound as prepared according to the methodology of this invention, and a pharmaceutically acceptable carrier.
Detailed Description of the Invention
The invention is directed to modulators, e.g., inhibitors, of Pinl and Pinl- related proteins and the use of such modulators for treatment of Pinl associated states, e.g., for the treatment of cancer.
In one embodiment, the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I):
Figure imgf000011_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1;
Z and Zi are independently selected from the group consisting of O or
-9- AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2O-, -S-, or -OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and - SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl-benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3-imino-l,3-dihydro-indol-2-one; (2,6-Dichloro- benzylidene)-imine; and 4-methyl-benzenesulfonamide; such that the Pinl -associated state is treated. In another embodiment, the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (II):
Figure imgf000012_0001
(II) wherein
R2, R3, and Re are independently selected from the group consisting of
H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, -CN, NO2, F, CF3,
OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4;
R4 is H or lower alkyl, e.g., CH3;
Xi, X2 and X3 are independently selected from the group consisting of
■10- -CH andN;
Ri is -(CH2)n-(X4)p-(CH2)m-Cθ2 5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, - CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Cι-C6 alkyl, e.g., CH3, CH2CH2SCH3, or OH; such that the Pinl -associated state is treated. In a specific embodiments, n=3, m=3 and p=0.
In one embodiment, the invention pertains, at least in part, to a method for treating a Pinl -associated state in a subject. The method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (III):
Figure imgf000013_0001
(in) wherein
R2, R3, and R are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, -CN, NO2, F, CF3, - OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R5 is H or lower alkyl, e.g., CH3;
Xi, X2 and X3 are independently selected from the group consisting of -CH and N; Ri is -(CH2)„-(X4)p-(CH2)m-Cθ2R6, wherein Re is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, - CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Cι-C6 alkyl, e.g., CH3, OH, or CH2CH2SCH3; such that the Pinl -associated state is treated. In a specific embodiments, n=3, m=3 and p=0. The term "Pinl-associated state" or "Pinl associated disorder" includes disorders and states (e.g., a disease state) which are associated with abnormal cell growth,
-11- abnormal cell proliferation, or aberrant levels of Pinl (e.g., Pinl protein or nucleic acid). Pinl -associated states include states resulting from an elevation in the expression of cyclin Dl and or Pinl. Pinl-associated states also include states resulting from an elevation in the phosphorylation level of c-Jun, particularly phosphorylation of c-Jun on Ser63/73-Pro and/or from an elevation in the level of c-Jun amino terminal kinases (JNKs) present in a cell. Pinl- associated states include neoplasia, cancer, undesirable cell growth, and/or tumor growth. Pinl-associated states include states caused by DNA damage, an oncogenic protein (i.e. Ha- Ras), loss of or reduced expression of a tumor suppressor (i.e. Brcal), and/or growth factors. Pinl is an important regulator of cyclin Dl expression. Due to Pinl 's role in regulating the expression of cyclin D 1 , many of the tumor causing effects of cyclin D 1 can be regulated through Pinl. In particular, inhibitors of Pinl can also be used to treat, inhibit, and/or prevent undesirable cell growth, e.g., tumors, neoplasia, and/or cancer associated with aberrant cyclin Dl expression in a subject.
Other examples of Pinl associated states include, but are not limited to, for example, those tumor types disclosed in Table 8.
The term "treated," "treating" or "treatment" includes the diminishment or alleviation of at least one symptom associated or caused by the state, disorder or disease being treated. In certain embodiments, the treatment comprises the induction of a Pinl inhibited state, followed by the activation of the Pinl modulating compound, which would in turn diminish or alleviate at least one symptom associated or caused by the Pinl associated state, disorder or disease being treated. For example, treatment can be diminishment of one or several symptoms of a disorder or complete eradication of a disorder.
The term "subject" is intended to include organisms, e.g., prokaryotes and eukaryotes, which are capable of suffering from or afflicted with a Pinl associated disorder. Examples of subjects include mammals, e.g. , humans, dogs, cows, horses, pigs, sheep, goats, cats, mice, rabbits, rats, and transgenic non-human animals. In certain embodiments, the subject is a human, e.g., a human suffering from, at risk of suffering from, or potentially capable of suffering from a Pinl associated disorder.
The language "Pinl modulating compound" refers to compounds that modulate, e.g., inhibit, promote, or otherwise alter, the activity of Pinl. Pinl modulating compounds include both Pinl agonists and antagonists. In certain embodiments, the Pinl modulating compound induces a Pinl inhibited-state. Examples of Pinl modulating compounds include compounds of formula (I), formula (II), and formula (III). Additional examples of Pinl modulating compounds include compounds of Table 1, Table 2, Table 3, Table 4, Table 5, Table 6, or derivatives thereof. In certain embodiments, the Pinl modulating compounds include compounds that interact with the PPI or the WW domain of
-12- Pinl. In certain embodiments, the Pinl modulating compound is substantially specific to Pinl. The phrase "substantially specific for Pinl" is intended to include inhibitors of the invention that have a Kj or K that is at least 2, 3, 4, 5, 10, 15, or 20 times less than the Ki or K for other peptidyl prolyl isomerases, e.g., hCyP-A, hCyP-B, hCyP-C, NKCA, hFKBP-12, hFKBP-13, and hFKBP-25.
In one embodiment of the invention, the Pinl modulating compound of the invention is capable of chemically interacting with Cysll3 of Pinl. The language "chemical interaction" is intended to include, but is not limited to reversible interactions such as hydrophobic/hydrophilic, ionic (e.g., coulombic attraction/repulsion, ion-dipole, charge- transfer), covalent bonding, Nan der Waals, and hydrogen bonding. In certain embodiments, the chemical interaction is a reversible Michael addition. In a specific embodiment, the Michael addition involves, at least in part, the formation of a covalent bond.
The language "Pinl inhibiting compound" includes compounds that reduce or inhibit the activity of Pinl. Examples of Pinl inhibiting compounds include compounds of formula (I), formula (II), and formula (III). Additional examples of Pinl inhibiting compounds include compounds of Table 1, Table 2, Table 3, Table 4, Table 5, Table 6, or derivatives thereof. In certain embodiments, the Pinl inhibiting compounds include compounds that interact with the PPI or the WW domain of Pinl .
In certain embodiments the inhibitors have a Ks for Pinl of less than 0.2mM, less than O.lmM, less than 750 μM, less than 500 μM, less than 250 μM, less than 100 μM, less than 50 μM, less than 500 nM, less than 250nM, less than 50 nM, less than 10 nM, less than 5 nM, or or less than 2 nM.
The language "Pinl inhibited-state" is intended to include states in which one activity of Pinl is inhibited in cells, e.g., cells in a subject, that have been treated with a Pinl modulating compound. "Pinl inbited-state is also intended to include states wherein the Pinl modulating compound is administered to a subject, allowed to remain in a preactivated state, and subsequently activated by a stimulus. The stimulus may be selected from a natural event, artificial event, or the combination thereof. For example, the natural event may be the action of an enzyme and/or the artificial event may be the addition of a hyperplastic inhibitory agent or the addition of energy to the subjects system in any manner that achieves activation, e.g. , by radiation, e.g., by light with a wavelength greater than about 400 nm, e.g., greater than about 600 nm, e.g., greater than about 620 nm, e.g., greater than about 630 nm, e.g., greater than about 640 nm, e.g., greater than about 650 nm. In one embodiment, the cells enter a Pinl inhibited-state for a designated period of time prior to activation of the modulating compound sufficient to allow the modulation the activity of Pinl by the activated modulating compound. In certain embodiments of the invention, the designated period of time prior to
-13- activation is greater than about 1 hour, e.g., greater than about 2 hours, e.g., greater than about 3 hours, e.g., greater than about 6 hours, e.g., greater than about 12 hours, e.g., greater than about 24 hours, e.g., greater than about 36 hours, e.g., greater than about 48 hours, e.g., greater than about 72 hours. In a specific embodiment, the designated period of time prior to activation is 3 days. In one embodiment, the Pinl modulating compound is preactivated prior to administration to a subject followed by the introduction of at least one stimulus sufficient to allow the modulation the activity of Pinl by the modulating compound. In certain embodiment of the invention, the activity of the modulating compound is enhanced by the entrance of the cells, e.g. , cells of a subject, into a Pinl inhibited state. In one embodiment of the invention, the Pinl modulating compounds of the invention have a characteristic inhibition profile (CIP) and have an effective cytotoxicity, e.g., effective to treat a Pinl associated state. The Pinl -modulating compounds described herein may be substituted with any substituent that allows the Pinl -modulating compound to perform its intended function. In certain embodiments the Pinl -modulating compounds described herein may be substituted with any substituent which allows the Pinl -modulating compound to perform its intended function, possess a CIP, and/or be effectively cytotoxic, as defined herein. The cytotoxicity of the compounds can be determined by using the CPCA given in Example 1. The measurement of the activity of the Pinl -modulating compounds in the determination the inhibition constant at 50% inhibition of enzyme activity (IC50), which is used to characterize the CIP, may be performed by using the analysis described in Example 2. An ordinarily skilled artisan would be able to use data generated by the assays to modify substituents on the Pinl modulating compounds to obtain effectively cytotoxic Pinl modulating compounds with characteristic inhibition profiles.
The term "characteristic inhibition profile (CIP)" is a characterization of the modulating compound of the invention such that the Pinl-associated state is inhibited. Characterization of the modulating compounds includes measurement of the inhibition constant at 50% inhibition of enzyme activity (IC50). Compounds that demonstrate a CIP include modulating compounds with and ICso of less than about 40 μM. In certain embodiments of the invention, the IC50 is between about 10-40 μM. In additional embodiments, the IC50 is between about 1-10 μM. In certain embodiments, the ICso is less than about 1 μM.
The term "effective cytotoxicity" or "effectively cytotoxic" includes cytotoxicities of Pinl -modulating compounds which allow the Pinl -modulating compound to perform its intended function, e.g., treat Pinl associated states. Cytotoxicities can be measured, for example, by using the Cell Based Cytotoxicity Assay (CBCA) method described in Example 1. In one embodiment, the Pinl -modulating compound has a
-14- cytotoxicity (as measured by the CBCA in Example 1) of 50 μM or less, 45 μM or less, 40 μM or less, 35 μM or less, 30 μM or less, 25 μM or less, 20 μM or less, 15 μM or less, 10 μM or less, 9 μM or less, 8 μM or less, 7 μM or less, 6 μM or less, 5 μM or less, 4 μM or less, 3 μM or less, 2 μM or less, 1 μM or less, 0.9 μM or less, 0.8 μM or less, 0.7 μM or less, 0.6 μM or less, 0.5 μM or less, 0.4 μM or less, or, preferably, 0.3 μM or less, or 0.05 μM or less. Values and ranges included and/or intermediate of the values set forth herein are also intended to be within the scope of the present invention.
In one embodiment, the Pinl modulating compounds of the invention are substantially soluble, e.g., water soluble, and have an effective cytotoxicity, e.g., effective to treat a Pinl associated state. Methods for altering the solubility of organic compounds are known in the art. For example, one of ordinary skill in the art will be able to modify the Pinl modulating compounds of the invention such that they have a desirable logP. Ordinarily skilled artisans will be able to modify the compounds by adding and removing hydrophilic and hydrophobic moieties, such that a Pinl -modulating compound with a desired solubility is obtained. The Pinl -modulating compounds described herein may be substituted with any substituent which allows the Pinl -modulating compound to perform its intended function, be substantially soluble, and/or be effectively cytotoxic, as defined herein. For example, an ordinarily skilled artisan would understand that the addition of heteroatoms (hydroxy, amino, nitro, carboxylic acid groups, etc.) or other polar moieties would generally increase the solubility of the Pinl modulating compound in water, while addition of non-polar moieties such as aryl or alkyl groups would generally decrease the solubility of the compound in water. The Pinl modulating compound can then be tested for substantial solubility by determining the logP value (e.g., by using a log octanol- water partition coefficient program such as "KOWWIN" (Meylan, W.M. and P.H. Howard. 1995. Atom/fragment contribution method for estimating octanol-water partition coefficients. J. Pharm. Sci. 84: 83-92, incorporated herein by reference in its entirety). An ordinarily skilled artisan would be able to use data generated by these programs and assays to modify substituents on the Pinl modulating compounds to obtain substantially soluble and effectively cytotoxic Pinl modulating compounds. The term "substantially soluble" includes solubilities (e.g., aqueous solubilities) of
Pinl -modulating compounds that allow the Pinl -modulating compounds to perform their intended function, e.g., treat Pinl associated states. The solubility of a particular Pinl- modulating compound can be measured by any method known in the art, e.g., experimentally, computationally, etc. For example, one method for determining the solubility of a compound computationally is by calculating logP values using a log octanol-water partition coefficient program (KOWWIN). In one embodiment, the Pinl -modulating compounds of the invention
-15- have logP values less than Pinl -modulating, e.g., less than 6.6. In a further embodiment, the Pinl -modulating compounds of the invention may have a logP value between about 1 to about 6, between about 1 to about 5, between about 1.5 to about 5, between about 2 to about 5, between about 2.5 to about 4.5, between about 2.75 to about 4.25, between about 3.0 to about 4.0, between about 3.25 to about 4.0, between about 3.5 to about 4.0, and between about 3.5 to about 3.75. Values and ranges included and/or intermediate of the values set forth herein are also intended to be within the scope of the present invention. In another embodiment, the aqueous solubility of the compound is about 0.01 mg/L or greater, about 0.1 mg/L or greater, about 1 mg/L or greater, or about 2 mg/L or greater. In certain embodiments of the invention, Z is S. In certain embodiments, Z\ is
O. In addition, in certain embodiments of the invention, n is selected from the group consisting of 0 through 5. Additionally, in particular embodiments of the invention, the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3 -(methylene)-l -methyl- 1,3 -dihydro-indol- 2-one, a benzofl ,3]dioxole, and Furazan 2-oxide.
The term "derivative" is intended to include isomers, modification, e.g., addition or removal, of substituents on the Pinl -modulating compound, and pharmaceutically acceptable salts thereof, as well as formulation, such that the Pinl -modulating compound treats the Pinl -associated state. The term "alkyl" includes saturated aliphatic groups, including straight-chain alkyl groups (e.g., methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, etc.), branched-chain alkyl groups (isopropyl, tert-butyl, isobutyl, etc.), cycloalkyl (alicyclic) groups (cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl), alkyl substituted cycloalkyl groups, and cycloalkyl substituted alkyl groups. The term alkyl further includes " alkyl groups, which can further include oxygen, nitrogen, sulfur or phosphorous atoms replacing one or more carbons of the hydrocarbon backbone. In an embodiment, a straight chain or branched chain alkyl has 10 or fewer carbon atoms in its backbone (e.g., Cr o for straight chain, C3-C10 for branched chain), and more preferably 6 or fewer. Likewise, preferred cycloalkyls have from 4-7 carbon atoms in their ring structure, and more preferably have 5 or 6 carbons in the ring structure.
Moreover, the term alkyl includes both "unsubstituted alkyls" and "substituted alkyls", the latter of which refers to alkyl moieties having substituents replacing a hydrogen on one or more carbons of the hydrocarbon backbone. Such substituents can include, for example, alkenyl, alkynyl, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl,
-16- alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, frifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moiety. Cycloalkyls can be further substituted, e.g., with the substituents described above. An "alkylaryl" or an "aralkyr' moiety is an alkyl substituted with an aryl (e.g., phenylmethyl (benzyl)). The term "alkyl" also includes the side chains of natural and unnatural amino acids. Examples of halogenated alkyl groups include fluoromethyl, difluoromethyl, trifluoromethyl, chloromethyl, dichloromethyl, trichloromethyl, perfluoromethyl, perchloromethyl, perfluoroethyl, perchloroethyl, etc.
The term "aryl" includes groups, including 5- and 6-membered single-ring aromatic groups that may include from zero to four heteroatoms, for example, benzene, phenyl, pyrrole, furan, thiophene, thiazole, isothiaozole, imidazole, triazole, tetrazole, pyrazole, oxazole, isooxazole, pyridine, pyrazine, pyridazine, and pyrimidine, and the like. Furthermore, the term "aryl" includes multicyclic aryl groups, e.g., tricyclic, bicyclic, e.g., naphthalene, benzoxazole, benzodioxazole, benzothiazole, benzoimidazole, benzothiophene, methylenedioxyphenyl, quinoline, isoquinoline, napthridine, indole, benzofuran, purine, benzofuran, deazapurine, or indolizine. Those aryl groups having heteroatoms in the ring structure may also be referred to as "aryl heterocycles", "heterocycles," "heteroaryls" or "heteroaromatics". The aromatic ring can be substituted at one or more ring positions with such substituents as described above, as for example, halogen, hydroxyl, alkoxy, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkylaminoacarbonyl, aralkylaminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylthiocarbonyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moiety. Aryl groups can also be fused or bridged with alicyclic or heterocyclic rings which are not aromatic so as to form a poly cycle (e.g., tetralin).
The term "alkenyl" includes unsaturated aliphatic groups analogous in length and possible substitution to the alkyls described above, but which contain at least one double bond.
-17- For example, the term "alkenyl" includes straight-chain alkenyl groups (e.g., ethenyl, propenyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, nonenyl, decenyl, etc.), branched-chain alkenyl groups, cycloalkenyl (alicyclic) groups (cyclopropenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl, cyclooctenyl), alkyl or alkenyl substituted cycloalkenyl groups, and cycloalkyl or cycloalkenyl substituted alkenyl groups. The term alkenyl further includes alkenyl groups that include oxygen, nitrogen, sulfur or phosphorous atoms replacing one or more carbons of the hydrocarbon backbone. In certain embodiments, a straight chain or branched chain alkenyl group has 6 or fewer carbon atoms in its backbone (e.g., C2-C6 for straight chain, C3-C6 for branched chain). Likewise, cycloalkenyl groups may have from 3-8 carbon atoms in their ring structure, and more preferably have 5 or 6 carbons in the ring structure. The term C2-C6 includes alkenyl groups containing 2 to 6 carbon atoms.
Moreover, the term alkenyl includes both "unsubstituted alkenyls" and "substituted alkenyls", the latter of which refers to alkenyl moieties having substituents replacing a hydrogen on one or more carbons of the hydrocarbon backbone. Such substituents can include, for example, alkyl groups, alkynyl groups, halogens, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moiety.
The term "alkynyl" includes unsaturated aliphatic groups analogous in length and possible substitution to the alkyls described above, but which contain at least one triple bond.
For example, the term "alkynyl" includes straight-chain alkynyl groups (e.g., ethynyl, propynyl, butynyl, pentynyl, hexynyl, heptynyl, octynyl, nonynyl, decynyl, etc.), branched-chain alkynyl groups, and cycloalkyl or cycloalkenyl substituted alkynyl groups. The term alkynyl further includes alkynyl groups that include oxygen, nitrogen, sulfur or phosphorous atoms replacing one or more carbons of the hydrocarbon backbone. In certain embodiments, a straight chain or branched chain alkynyl group has 6 or fewer carbon atoms in its backbone (e.g., C2-C6 for straight chain, C3-C6 for branched chain). The term C2-C6 includes alkynyl groups containing 2 to 6 carbon atoms. Moreover, the term alkynyl includes both "unsubstituted alkynyls" and
"substituted alkynyls", the latter of which refers to alkynyl moieties having substituents
-18- replacing a hydrogen on one or more carbons of the hydrocarbon backbone. Such substituents can include, for example, alkyl groups, alkynyl groups, halogens, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moiety.
Unless the number of carbons is otherwise specified, "lower alkyl" as used herein means an alkyl group, as defined above, but having from one to five carbon atoms in its backbone structure. "Lower alkenyl" and "lower alkynyl" have chain lengths of, for example, 2-5 carbon atoms. The term "acyl" includes compounds and moieties which contain the acyl radical (CH3CO-) or a carbonyl group. The term "substituted acyl" includes acyl groups where one or more of the hydrogen atoms are replaced by for example, alkyl groups, alkynyl groups, halogens, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moiety.
The term "acylamino" includes moieties wherein an acyl moiety is bonded to an amino group. For example, the term includes alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido groups. The term "aroyi" includes compounds and moieties with an aryl or heteroaromatic moiety bound to a carbonyl group. Examples of aroyl groups include phenylcarboxy, naphthyl carboxy, etc.
The terms "alkoxyalkyl", "alkylaminoalkyl" and "thioalkoxyalky ' include alkyl groups, as described above, which further include oxygen, nitrogen or sulfur atoms replacing one or more carbons of the hydrocarbon backbone, e.g., oxygen, nitrogen or sulfur atoms.
-19- The term "alkoxy" includes substituted and unsubstituted alkyl, alkenyl, and alkynyl groups covalently linked to an oxygen atom. Examples of alkoxy groups include methoxy, ethoxy, isopropyloxy, propoxy, butoxy, and pentoxy groups and may include cyclic groups such as cyclopentoxy. Examples of substituted alkoxy groups include halogenated alkoxy groups. The alkoxy groups can be substituted with groups such as alkenyl, alkynyl, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moieties. Examples of halogen substituted alkoxy groups include, but are not limited to, fluoromethoxy, difluoromethoxy, trifluoromethoxy, chloromethoxy, dichloromethoxy, trichloromethoxy, etc.
The term "amine" or "amino" includes compounds where a nitrogen atom is covalently bonded to at least one carbon or heteroatom. The term "alkyl amino" includes groups and compounds wherein the nitrogen is bound to at least one additional alkyl group. The term "dialkyl amino" includes groups wherein the nitrogen atom is bound to at least two additional alkyl groups. The term "arylamino" and "diarylamino" include groups wherein the nitrogen is bound to at least one or two aryl groups, respectively. The term "alkylarylamino," "alkylarninoaryi" or "aryla inoalkyi" refers to an amino group that is bound to at least one alkyl group and at least one aryl group. The term "alkaminoalkyl" refers to an alkyl, alkenyl, or alkynyl group bound to a nitrogen atom that is also bound to an alkyl group.
The term "amide" or "aminocarboxy" includes compounds or moieties that contain a nitrogen atom that is bound to the carbon of a carbonyl or a thiocarbonyl group. The term includes "alkaminocarboxy" groups that include alkyl, alkenyl, or alkynyl groups bound to an amino group bound to a carboxy group. It includes arylaminocarboxy groups that include aryl or heteroaryl moieties bound to an amino group which is bound to the carbon of a carbonyl or thiocarbonyl group. The terms "alkylaminocarboxy," "alkenylaminocarboxy," "alkynylaminocarboxy," and "arylaminocarboxy" include moieties wherein alkyl, alkenyl, alkynyl and aryl moieties, respectively, are bound to a nitrogen atom which is in turn bound to the carbon of a carbonyl group.
-20- The term "carbonyl" or "carboxy" includes compounds and moieties which contain a carbon connected with a double bond to an oxygen atom, and tautomeric forms thereof. Examples of moieties that contain a carbonyl include aldehydes, ketones, carboxylic acids, amides, esters, anhydrides, etc. The term "carboxy moiety" or "carbonyl moiety" refers to groups such as "alkylcarbonyl" groups wherein an alkyl group is covalently bound to a carbonyl group, "alkenylcarbonyi" groups wherein an alkenyl group is covalently bound to a carbonyl group, "alkynylcarbonyl" groups wherein an alkynyl group is covalently bound to a carbonyl group, "arylcarbonyl" groups wherein an aryl group is covalently attached to the carbonyl group. Furthermore, the term also refers to groups wherein one or more heteroatoms are covalently bonded to the carbonyl moiety. For example, the term includes moieties such as, for example, aminocarbonyl moieties, (wherein a nitrogen atom is bound to the carbon of the carbonyl group, e.g., an amide), aminocarbonyloxy moieties, wherein an oxygen and a nitrogen atom are both bond to the carbon of the carbonyl group (e.g., also referred to as a "carbamate"). Furthermore, aminocarbonylamino groups (e.g., ureas) are also include as well as other combinations of carbonyl groups bound to heteroatoms (e.g. , nitrogen, oxygen, sulfur, etc. as well as carbon atoms). Furthermore, the heteroatom can be further substituted with one or more alkyl, alkenyl, alkynyl, aryl, aralkyl, acyl, etc. moieties. The term "thiocarbonyl" or "thiocarboxy" includes compounds and moieties which contain a carbon connected with a double bond to a sulfur atom. The term "thiocarbonyl moiety" includes moieties that are analogous to carbonyl moieties. For example, "thiocarbonyl" moieties include aminothiocarbonyl, wherein an amino group is bound to the carbon atom of the thiocarbonyl group, furthermore other thiocarbonyl moieties include, oxythiocarbonyls (oxygen bound to the carbon atom), aminothiocarbonylamino groups, etc. The term "ether" includes compounds or moieties that contain an oxygen bonded to two different carbon atoms or heteroatoms. For example, the term includes "alkoxyalkyl" which refers to an alkyl, alkenyl, or alkynyl group covalently bonded to an oxygen atom that is covalently bonded to another alkyl group.
The term "ester" includes compounds and moieties that contain a carbon or a heteroatom bound to an oxygen atom that is bonded to the carbon of a carbonyl group. The term "ester" includes alkoxycarboxy groups such as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, butoxycarbonyl, pentoxy carbonyl, etc. The alkyl, alkenyl, or alkynyl groups are as defined above.
The term "thioether" includes compounds and moieties which contain a sulfur atom bonded to two different carbon or hetero atoms. Examples of thioethers include, but are not limited to alkthioalkyls, alkthioalkenyls, and alkthioalkynyls. The term "alkthioalkyls"
-21- include compounds with an alkyl, alkenyl, or alkynyl group bonded to a sulfur atom that is bonded to an alkyl group. Similarly, the term "alkthioalkenyls" and alkthioalkynyls" refer to compounds or moieties wherein an alkyl, alkenyl, or alkynyl group is bonded to a sulfur atom which is covalently bonded to an alkynyl group. The term "hydroxy" or "hydroxyl" includes groups with an -OH or -O".
The term "halogen" includes fluorine, bromine, chlorine, iodine, etc. The term "perhalogenated" generally refers to a moiety wherein all hydrogens are replaced by halogen atoms.
The terms "polycyclyl" or "polycyclic radical" include moieties with two or more rings (e.g., cycloalkyls, cycloalkenyls, cycloalkynyls, aryls and/or heterocyclyls) in which two or more carbons are common to two adjoining rings, e.g., the rings are "fused rings". Rings that are joined through non-adjacent atoms are termed "bridged" rings. Each of the rings of the polycycle can be substituted with such substituents as described above, as for example, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkoxycarbonyl, alkylaminoacarbonyl, aralkylaminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, alkenylcarbonyl, aminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkyl, alkylaryl, or an aromatic or heteroaromatic moiety.
The term "heteroatom" includes atoms of any element other than carbon or hydrogen. Preferred heteroatoms are nitrogen, oxygen, sulfur and phosphorus. The term "heterocycle" or "heterocyclic" includes saturated, unsaturated, aromatic ("heteroaryls" or "heteroaromatic") and polycyclic rings which contain one or more heteroatoms. Examples of heterocycles include, for example, benzodioxazole, benzofuran, benzoimidazole, benzothiazole, benzothiophene, benzoxazole, deazapurine, furan, indole, indolizine, imidazole, isooxazole, isoquinoline, isothiaozole, methylenedioxyphenyl, napthridine, oxazole, purine, pyrazine, pyrazole, pyridazine, pyridine, pyrimidine, pyrrole, quinoline, tetrazole, thiazole, thiophene, and triazole. Other heterocycles include morpholine, piprazine, piperidine, thiomorpholine, and thioazolidine. The heterocycles may be substituted or unsubstituted. Examples of substituents include, for example, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, alkoxycarbonyl, alkylaminoacarbonyl, aralkylaminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, aralkylcarbonyl, alkenylcarbonyl,
-22- aminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkyl, alkylaryl, or an aromatic or heteroaromatic moiety.
It will be noted that the structures of some of the compounds of this invention include asymmetric carbon atoms. It is to be understood accordingly that the isomers arising from such asymmetry (e.g., all enantiomers and diastereomers) are included within the scope of this invention, unless indicated otherwise. Such isomers can be obtained in substantially pure form by classical separation techniques and by stereochemically controlled synthesis. Furthermore, the structures and other compounds and moieties discussed in this application also include all tautomers thereof. Compounds described herein may be obtained though art recognized synthesis strategies.
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-87 In a particular embodiment of the invention, the Pinl modulating compound of formula (I) is any one of the compounds of Table 1, Table 2, Table 3, Table 4, Table 5, Table 6, or derivatives thereof.
In another embodiment, the invention pertains to the Pinl -modulating compounds of formula (I), formula (II), or formula (III) described herein. Particular embodiments of the invention pertain to the modulating compounds of Table 1, Table 2, Table 3, Table 4, Table 5, Table 6, or derivatives thereof.
In yet another embodiment, the invention pertains to pharmaceutical compositions comprising the Pinl -modulating compounds described herein and a pharmaceutical acceptable carrier.
The another embodiment, the invention is intended to include any novel compound described herein.
Additionally, the compounds described above are intended to include analogs containing art-recognized substituents that do not significantly effect the analog's ability to perform its intended function
In an additional embodiment, the invention pertains, at least in part, to a method for treating cyclin Dl overexpression in a subject. This method includes administering to the subject an effective amount of a Pinl -modulating compound of formula (I), formula (II), or formula (III), as described above, such that the cyclin Dl overexpression is treated. In certain embodiments, the overexpression of cyclin Dl is associated with the presence of breast cancer in the subject.
"Increased cyclin Dl expression" or "cyclin Dl overexpression" or "elevation in. the expression of cyclin Dl" includes cells having higher than normal levels of cyclin Dl. Significant cyclin Dl overexpression includes both small and large increases in the levels of cyclin Dl compared with normal levels. Preferably, cyclin Dl overexpression is considered in the context of the phase of the cell cycle. In actively proliferating normal cells, cyclin Dl reaches a peak in mid Gi phase, decreases during S-phase, and remains low throughout the rest of the cycle, however, in transformed cells the level of cyclin Dl is more variable. Therefore, cyclin Dl overexpression includes the expression of cyclin Dl at levels that are abnormally high for the particular cell cycle phase of the cell. Cyclin Dl overexpression can manifest itself as tumor growth or cancer. One skilled in the art would recognize the comparative studies that have been done measuring the level of cyclin Dl expression in normal cells in comparison with cells having a cancerous state. Increased cyclin D 1 expression has been found in a vast range of primary human tumors. Increased cyclin Dl expression has been detected in the form of gene amplification, increased cyclin Dl RNA expression, and increased cyclin Dl protein expression. Most clinical studies comparing cyclin Dl gene amplification with
-88- expression of cyclin Dl have found that more cases show over-expression of both RNA and protein than show amplification of the gene. The presence of increased cyclin Dl RNA and/or protein expression without gene amplification suggests that other cellular genes such as pRb may affect the expression cyclin Dl . Human tumors found to have increased cyclin Dl expression include: parathyroid adenomas, mantle cell lymphomas, breast cancers, head and neck squamous cell carcinomas (i.e. squamous carcinomas in the oral cavity, nasopharynx, pharynx, hypopharynx, and larynx), esophageal cancers, hepatocellular carcinomas, colorectal cancers, genitourinary cancers, lung cancers (i.e. squamous cell carcinomas of the lung), skins cancers (i.e. squamous cell carcinomas, melanomas, and malignant fibrous histiocytomas), sarcomas, and central nervous system malignancies (i.e. astrocytomas and glioblastomas), gastric adenocarcinomas, pancreatic adenocarcinomas, squamous carcinomas of the gall bladder (Donnellan, etal. 1998. J. Clin. Pathol: Mol. Pathol 51:1-7). The cyclin Dl gene is amplified in approximately 20% of mammary carcinomas and the protein is overexpressed in approximately 50% of mammary carcinomas (Barnes, et al. 1998, Breast Cancer Research and Treatment.
52:1-15). Cyclin Dl overexpression in mantle cell lymphoma is discussed in Espinet, et a\. 1999. Cancer Genet Cytogenet. lll(l):92-8 and Stamatopoulous, et al. 1999. Br. J. Haematol 105(1): 190-7. Cyclin Dl overexpression in breast cancer is discussed in Fredersdorf, et al. 1997. EN4S 94(12):6380-5. Cyclin Dl overexpression in head and neck cancers is discussed in Matthias, et al 1999. Cancer Epidemiol. Biomarkers Prev. 8(9):815-23; Matthias, et al. 1998. Clin. Cancer Res. 4(10):2411-8; and Kyomcto, et al. 1997. Int. J. Cancer. 74(6):576-81. Cyclin Dl overexpression in laryngeal carcinoma is discussed in Bellacosa, et al. 1996. Clin. Cancer Res. 2(l):175-80. Cyclin Dl overexpression in multiple myeloma is discussed in Hoechtlen-Nollmar, et al. 2000. Br. J. Haematol 109(l):30-8; Pruneri, et al. 2000. Am. J. Pathol. 156(5): 1505-13; and
Janssen, et al. 2000. Blood 95(8):2691-8. It is believed that in many tumors, cyclin Dl acts in co-operation with other oncogenes or tumor suppressor genes.
Cyclin Dl expression is regulated by many factors. Growth factors (i.e. CSF1, platelet-derived growth factor, insulin-like growth factor, steroid hormones, prolactin, and serum stimulation) promote the synthesis of cyclin Dl and removal of growth factors lead to a drop in cyclin Dl levels and arrest the cell in Gi. Hosokawa, et al 1996. J. Lab. Clin. Med. 127:246-52. While hypophosphorylated pRb stimulates cyclin Dl transcription, cyclin Dl activity is inhibited by transforming growth factor β- 1, p53, and cyclin dependent kinase inhibitors (CKIs). High levels of CKIs bind to cdks and reduce the ability of cyclins to activate the cdks. The two classes of CKIs are the Kip/Cip family including p21, p27, and p57, capable of binding to and inhibiting most cyclin-cdk complexes, and the IΝK4 family including pl5, pl6, 18, and pl9, which seem to be specific inhibitors of cyclin Dl-cdk complexes. Donnellan, et al. 1998. J.
-89- Clin. Pathol: Mol. Pathol 51:1-7. In addition, CKI ρl6 is activated by pRb and E2F, while the levels of CKI p27 are increased by TGF-β, cAMP, contact inhibition, and serum deprivation. Barnes, etal. 1998. Breast Cancer Research and Treatment. 52:1- 15. Cyclin Dl is believed to act through the phosphorylation of pRB, which is hypophosphorylated throughout the Gi phase, phosphorylated just before the S phase, and remains phosphorylated until late mitosis. Hypophosphorylated pRB arrests cells in Gi by forming a complex with the E2F family of DNA binding proteins that transcribe genes associated with DNA replication (the S phase of the cell cycle). Cyclin D 1 can form a complex with either cdk4 or cdk6 to form activated cdk4 or cdk6. Activated cdk4 or cdk6 induces the phosphorylation of pRb changing pRb from its hypophosphorylated form in which it binds to and inactivates E2F transcription factors to phosphorylated pRb that no longer binds to nor inactivates E2F transcription factors. In some mouse lymphoma cells overexpressing D cyclins, pRb is hyperphosphorylated compared with pRb in cells not overexpressing D c clins. It appears that cyclin Dl is required to initiate the phosphorylation of pRb that, in turn, drives the cell through the restriction point at which stage the cell is committed to divide.
"Neoplasia" or "neoplastic transformation" is the pathologic process that results in the formation and growth of a neoplasm, tissue mass, or tumor. Such process includes uncontrolled cell growth, including either benign or malignant tumors. Neoplasms include abnormal masses of tissue, the growth of which exceeds and is uncoordinated with that of the normal tissues and persists in the same excessive manner after cessation of the stimuli that evoked the change. Neoplasms may show a partial or complete lack of structural organization and functional coordination with the normal tissue, and usually form a distinct mass of tissue. One cause of neoplasia is dysregulation of the cell cycle machinery.
Neoplasms tend to grow and function somewhat independently of the homeostatic mechanisms that control normal tissue growth and function. However, some neoplasms remain under the control of the homeostatic mechanisms that control normal tissue growth and function. For example, some neoplasms are estrogen sensitive and can be arrested by anti-estrogen therapy. Neoplasms can range in size from less than 1 cm to over 6 inches in diameter. A neoplasm even 1 cm in diameter can cause biliary obstructions and jaundice, if it arises in and obstructs the ampulla of Nater. Neoplasms tend to morphologically and functionally resemble the tissue from which they originated. For example, neoplasms arising within the islet tissue of the pancreas resemble the islet tissue, contain secretory granules, and secrete insulin. Clinical features of a neoplasm may result from the function of the tissue from which it
-90- originated. For example, excessive amounts of insulin can be produced by islet cell neoplasms resulting in hypoglycemia which, in turn, results in headaches and dizziness. However, some neoplasms show little morphological or functional resemblance to the tissue from which they originated. Some neoplasms result in such non-specific systemic effects as cachexia, increased susceptibility to infection, and fever.
By assessing the histology and other features of a neoplasm, it can be determined whether the neoplasm is benign or malignant. Invasion and metastasis (the spread of the neoplasm to distant sites) are definitive attributes of malignancy. Despite the fact that benign neoplasms may attain enormous size, they remain discrete and distinct from the adjacent non-neoplastic tissue. Benign tumors are generally well circumscribed and round, have a capsule, and have a grey or white color, and a uniform texture. In contrast, malignant tumors generally have fingerlike projections, irregular margins, are not circumscribed, and have a variable color and texture. Benign tumors grow by pushing on adjacent tissue as they grow. As the benign tumor enlarges it compresses adjacent tissue, sometimes causing atrophy. The junction between a benign tumor and surrounding tissue may be converted to a fibrous connective tissue capsule allowing for easy surgical removal of the benign tumor.
Conversely, malignant tumors are locally invasive and grow into the adjacent tissues usually giving rise to irregular margins that are not encapsulated making it necessary to remove a wide margin of normal tissue for the surgical removal of malignant tumors. Benign neoplasms tend to grow more slowly and tend to be less autonomous than malignant tumors. Benign neoplasms tend to closely histologically resemble the tissue from which they originated. More highly differentiated cancers, i. e., cancers that resemble the tissue from which they originated, tend to have a better prognosis than poorly differentiated cancers, while malignant tumors are more likely than benign tumors to have an aberrant function, e.g., the secretion of abnormal or excessive quantities of hormones.
The histological features of cancer are summarized by the term "anaplasia." Malignant neoplasms often contain numerous mitotic cells. These cells are typically abnormal. Such mitotic aberrations account for some of the karyotypic abnormalities found in most cancers. Bizarre multinucleated cells are also seen in some cancers, especially those that are highly anaplastic.
The term "anaplasia" includes histological features of cancer. These features include derangement of the normal tissue architecture, the crowding of cells, lack of cellular orientation termed dyspolarity, and cellular heterogeneity in size and shape termed "pleomorphism." The cytologic features of anaplasia include an increased nuclear-cytoplasmic ratio (nuclear-cytoplasmic ratio can be over 50% for malignant cells), nuclear pleomorphism, clumping of the nuclear chromatin along the nuclear
-91- membrane, increased staining of the nuclear chromatin, simplified endoplasmic reticulum, increased free ribosomes, pleomorphism of mitochondria, decreased size and number of organelles, enlarged and increased numbers of nucleoli, and sometimes the presence of intermediate filaments. The term "dysplasia" includes pre-malignant states in which a tissue demonstrates histologic and cytologic features intermediate between normal and anaplastic. Dysplasia is often reversible.
The term "cancer" includes malignancies characterized by deregulated or uncontrolled cell growth, for instance carcinomas, sarcomas, leukemias, and lymphomas. The term "cancer" includes primary malignant tumors, e.g. , those whose cells have not migrated to sites in the subject's body other than the site of the original tumor, and secondary malignant tumors, e.g., those arising from metastasis, the migration of tumor cells to secondary sites that are different from the site of the original tumor. The term "carcinoma" includes malignancies of epithelial or endocrine tissues, including respiratory system carcinomas, gastrointestinal system carcinomas, genitourinary system carcinomas, testicular carcinomas, breast carcinomas, prostate carcinomas, endocrine system carcinomas, melanomas, choriocarcinoma, and carcinomas of the cervix, lung, head and neck, colon, and ovary. The term "carcinoma" also includes carcinosarcomas, which include malignant tumors composed of carcinomatous and sarcomatous tissues. The term "adenocarcmoma" includes carcinomas derived from glandular tissue or a tumor in which the tumor cells form recognizable glandular structures.
The term "sarcoma" includes malignant tumors of mesodermal connective tissue, e.g., tumors of bone, fat, and cartilage.
The terms "leukemia" and "lymphoma" include malignancies of the hematopoietic cells of the bone marrow. Leukemias tend to proliferate as single cells, whereas lymphomas tend to proliferate as solid tumor masses. Examples of leukemias include acute myeloid leukemia (AML), acute promyelocytic leukemia, chronic myelogenous leukemia, mixed-lineage leukemia, acute monoblastic leukemia, acute lymphoblastic leukemia, acute non-lymphoblastic leukemia, blastic mantle cell leukemia, myelodyplastic syndrome, T cell leukemia, B cell leukemia, and chronic lymphocytic leukemia. Examples of lymphomas include Hodgkin's disease, non- Hodgkin's lymphoma, B cell lymphoma, epitheliotropic lymphoma, composite lymphoma, anaplastic large cell lymphoma, gastric and non-gastric mucosa-associated lymphoid tissue lymphoma, lymphoproliferative disease, T cell lymphoma, Burkitt's lymphoma, mantle cell lymphoma, diffuse large cell lymphoma, Iymphoplasmacytoid lymphoma, and multiple myeloma.
-92- For example, the therapeutic methods of the present invention can be applied to cancerous cells of mesenchymal origin, such as those producing sarcomas (e.g., fibrosarcoma, myxosarcoma, liosarcoma, chondrosarcoma, osteogenic sarcoma or chordosarcoma, angiosarcoma, endotheliosardcoma, lympangiosarcoma, synoviosarcoma or mesothelisosarcoma); leukemias and lymphomas such as granulocytic leukemia, monocytic leukemia, lymphocytic leukemia, malignant lymphoma, plasmocytoma, reticulum cell sarcoma, or Hodgkin's disease; sarcomas such as leiomysarcoma or rhabdomysarcoma, tumors of epithelial origin such as squamous cell carcinoma, basal cell carcinoma, sweat gland carcinoma, sebaceous gland carcinoma, adenocarcmoma, papillary carcinoma, papillary adenocarcmoma, cystadenocarcinoma, medullary carcinoma, undifferentiated carcinoma, bronchogenic carcinoma, melanoma, renal cell carcinoma, hepatoma-liver cell carcinoma, bile duct carcinoma, cholangiocarcinoma, papillary carcinoma, transitional cell carcinoma, chorioaencinoma, semonoma, or embryonal carcinoma; and tumors of the nervous system including gioma, menigoma, medulloblastoma, schwannoma or epidymoma. Additional cell types amenable to treatment according to the methods described herein include those giving rise to mammary carcinomas, gastrointestinal carcinoma, such as cόlonic carcinomas, bladder carcinoma, prostate carcinoma, and squamous cell carcinoma of the neck and head region. Examples of cancers amenable to treatment according to the methods described herein include vaginal, cervical, and breast cancers. The language "inhibiting undesirable cell growth" is intended to include the inhibition of undesirable or inappropriate cell growth. The inhibition is intended to include inhibition of proliferation including rapid proliferation. For example, the cell growth can result in benign masses or the inhibition of cell growth resulting in malignant tumors. Examples of benign conditions which result from inappropriate cell growth or angiogenesis are diabetic retinopathy, retrolental fibrioplasia, neovascular glaucoma, psoriasis, angiofibromas, rheumatoid arthritis, hemangiomas, Karposi's sarcoma, and other conditions or dysfunctions characterized by dysregulated endothelial cell division. The language "inhibiting tumor growth" or "inhibiting neoplasia" includes the prevention of the growth of a tumor in a subject or a reduction in the growth of a pre-existing tumor in a subject. The inhibition also can be the inhibition of the metastasis of a tumor from one site to another. In particular, the language "tumor" is intended to encompass both in vitro and in vivo tumors that form in any organ or body part of the subject. The tumors preferably are tumors sensitive to the Pinl -modulating compounds of the present invention. Examples of the types of tumors intended to be encompassed by the present invention include those tumors associated with breast cancer, skin cancer, bone cancer, prostate cancer, liver cancer, lung cancer, brain cancer, cancer of the larynx, gallbladder, esophagus, pancreas, rectum, parathyroid, thyroid,
-93- adrenal, neural tissue, head and neck, colon, stomach, bronchi, kidneys. Specifically, the tumors whose growth rate is inhibited by the present invention include basal cell carcinoma, squamous cell carcinoma of both ulcerating and papillary type, metastatic skin carcinoma, osteo sarcoma, Ewing's sarcoma, veticulum cell sarcoma, myeloma, giant cell tumor, small-cell lung tumor, gallstones, islet cell tumor, primary brain tumor, acute and chronic lymphocytic and granulocytic tumors, hairy-cell tumor, adenoma, hyperplasia, medullary carcinoma, pheochromocytoma, mucosal neuromas, intestinal ganglloneuromas, hyperplastic corneal nerve tumor, marfanoid habitus tumor, Wilm's tumor, seminoma, ovarian tumor, leiomyomater tumor, cervical dysplasia and in situ carcinoma, neuroblastoma, retinoblastoma, soft tissue sarcoma, malignant carcinoid, topical skin lesion, mycosis fungoide, rhabdomyosarcoma, Kaposi's sarcoma, osteogenic and other sarcoma, malignant hypercalcemia, renal cell tumor, polycythermia vera, adenocarcinoma, glioblastoma multiforma, leukemias, lymphomas (i.e. maglinant lymphomas, mantle cell lymphoma), malignant melanomas, multiple myeloma, epidermoid carcinomas, and other carcinomas and sarcomas.
The Pinl modulating compounds of the present invention may be used to treat, inhibit, and/or prevent undesirable cell growth, neoplasia, and/or cancer in any subject. The Pinl modulating compounds of the present invention may be used to inhibit Pinl activity in a subject. In one embodiment, the Pinl modulating compounds of the present invention may be used to inhibit cyclin Dl expression in a subject.
In one embodiment, the invention pertains, at least in part, to a method fortreating a Pinl-associated state in a subject. The method includes administering to a subject an effective amount of a combination of a Pinl modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, and a hyperplastic inhibitory agent to treat the Pinl associated states.
In another embodiment, the invention pertains, at least in part, to a method for treating cyclin Dl overexpression in a subject. The method includes administering to a subject an effective amount of a combination of a Pinl modulating compound of the invention, e.g. , Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, and a hyperplastic inhibitory agent to treat the cyclin Dl overexpression.
In yet another embodiment, the invention pertains, at least in part, to a method for treating cancer in a subject. The method includes administering to a subject an effective amount of a combination of a Pinl modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, and a hyperplastic inhibitory agent to treat the cancer.
-94. The language "hyperplastic inhibitory agent" includes agents that inhibit the growth of proliferating cells or tissue wherein the growth of such cells or tissues is undesirable. For example, the inhibition can be of the growth of malignant cells, such as in neoplasms or benign cells, e.g., in tissues where the growth is inappropriate. Examples of the types of agents that can be used include chemotherapeutic agents, radiation therapy treatments, including therapeutically effective ranges of light, e.g. , laser light and/or immunofluorescent compounds, and associated radioactive compounds and methods, immunotoxins, and combinations thereof.
The language "chemotherapeutic agent" includes chemical reagents that inhibit the growth of proliferating cells or tissues wherein the growth of such cells or tissues is undesirable. Chemotherapeutic agents are well known in the art (see e.g., Gilman A.G., et al, The Pharmacological Basis of Therapeutics, 8th Ed., Sec 12:1202- 1263 (1990)), and are typically used to treat neoplastic diseases. The chemotherapeutic agents generally employed in chemotherapy treatments are listed below in Table 7. Other similar examples of chemotherapeutic agents include: bleomycin, docetaxel (Taxotere), doxorubicin, edatrexate, etoposide, finasteride (Proscar), flutamide (Eulexin), gemcitabine (Gemzar), goserelin acetate (Zoladex), granisetron (Kytril), irinotecan (Campto/Camptosar), ondansetron (Zofran), paclitaxel (Taxol), pegaspargase (Oncaspar), pilocarpine hydrochloride (Salagen), porfimer sodium (Photofrin), interleukin-2 (Proleukin), rituximab (Rituxan), topotecan (Hycamtin), trastuzumab (Herceptin), tretinoin (Retin-A), Triapine, vincristine, and vinorelbine tartrate (Navelbine).
-95- TABLE 7
Figure imgf000098_0001
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Figure imgf000099_0001
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Figure imgf000100_0001
The language "radiation therapy" includes the application of a genetically and somatically safe level of electrons, protons, or photons, both localized and non- localized, to a subject to inhibit, reduce, or prevent symptoms or conditions associated with undesirable cell growth. The term X-rays is also intended to include machine- generated radiation, clinically acceptable radioactive elements, and isotopes thereof, as well as the radioactive emissions therefrom. Examples of the types of emissions include alpha rays, beta rays including hard betas, high-energy electrons, and gamma rays. Radiation therapy is well known in the art (see e.g. , Fishbach, F., Laboratory Diagnostic Tests, 3rd Ed., Ch. 10: 581-644 (1988)), and is typically used to treat neoplastic diseases.
The term "immunotoxins" includes immunotherapeutic agents that employ cytotoxic T cells and/or antibodies, e.g., monoclonal, polyclonal, phage antibodies, or fragments thereof, which are utilized in the selective destruction of undesirable rapidly proliferating cells. For example, immunotoxins can include antibody-toxin conjugates (e.g., Ab-ricin and Ab-diptheria toxin), antibody-radiolabels (e.g., Ab-ll3 ) ^d antibody activation of the complement at the tumor cell. The use of immunotoxins to inhibit, reduce, or prevent symptoms or conditions associated with neoplastic diseases are well known in the art (see, e.g., Harlow, E. and Lane, D., Antibodies, (1988)).
In one embodiment, the invention includes a packaged Pinl-associated state treatment. The packaged treatment includes a Pinl modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, packaged with instructions for using an effective amount of the
-98- Pinl modulating compound.
In another embodiment, the invention includes a packaged cyclin Dl overexpression treatment. This packaged treatment include a Pinl modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, packaged with instructions for using an effective amount of the Pinl modulating compound to treat cyclin Dl overexpression.
In yet another embodiment, the invention also pertains, at least in part to a packaged cancer treatment, which includes a Pinl -modulating compound of the invention, e.g., Pinl -modulating compounds of formula (I), formula (II), or formula (III), as described above, packaged with instructions for using an effective amount of the Pinl -modulating compound to treat cancer.
The invention also pertains, at least in part, to pharmaceutical compositions of comprising Pinl -modulating compounds of the invention, e.g., Pinl- modulating compounds of formula (I), formula (II), or formula (III), as described above, and, optionally, a pharmaceutically acceptable carrier.
The language "effective amount" of the compound is that amount necessary or sufficient to treat or prevent a Pinl associated state, e.g. prevent the various morphological and somatic symptoms of a Pinl associated state. In an example, an effective amount of the Pinl -modulating compound is the amount sufficient to inhibit undesirable cell growth in a subject. In another example, an effective amount of the
Pinl -modulating compound is the amount sufficient to reduce the size of a pre-existing benign cell mass or malignant tumor in a subject. The effective amount can vary depending on such factors as the size and weight of the subject, the type of illness, or the particular Pinl binding compound. For example, the choice of the Pinl binding compound can affect what constitutes an "effective amount". One of ordinary skill in the art would be able to study the factors contained herein and make the determination regarding the effective amount of the Pinl binding compound without undue experimentation. In one possible assay, an effective amount of a Pinl -modulating compound can be determined by assaying for the expression of cyclin Dl and determining the amount of the Pinl -modulating compound sufficient to reduce the levels of cyclin Dl to that associated with a non-cancerous state.
The regimen of administration can affect what constitutes an effective amount. The Pinl binding compound can be administered to the subject either prior to or after the onset of a Pinl associated state. Further, several divided dosages, as well as staggered dosages, can be administered daily or sequentially, or the dose can be continuously infused, or can be a bolus injection. Further, the dosages of the Pinl binding compound(s) can be proportionally increased or decreased as indicated by the exigencies of the therapeutic or prophylactic situation.
-99- The language "pharmaceutical composition" includes preparations suitable for administration to mammals, e.g., humans. When the compounds of the present invention are administered as pharmaceuticals to mammals, e.g., humans, they can be given per se or as a pharmaceutical composition containing, for example, 0.1 to 99.5% (more preferably, 0.5 to 90%) of active ingredient in combination with a pharmaceutically acceptable carrier.
The phrase "pharmaceutically acceptable carrier" is art recognized and includes a pharmaceutically acceptable material, composition or vehicle, suitable for administering compounds of the present invention to mammals. The carriers include liquid or solid filler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting the subject agent from one organ, or portion of the body, to another organ, or portion of the body. Each carrier must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not injurious to the patient. Some examples of materials which can serve as pharmaceutically acceptable carriers include: sugars, such as lactose, glucose and sucrose; starches, such as corn starch and potato starch; cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar; buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol; phosphate buffer solutions; and other non-toxic compatible substances employed in pharmaceutical formulations. Wetting agents, emulsifiers and lubricants, such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, release agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the compositions.
Examples of pharmaceutically acceptable antioxidants include: water soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfϊte, sodium sulfite and the like; oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecithin, propyl gallate, α-tocopherol, and the like; and metal chelating agents, such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like.
Formulations of the present invention include those suitable for oral, nasal, topical, transdermal, buccal, sublingual, rectal, vaginal and/or parenteral administration. The formulations may conveniently be presented in unit dosage form and
-100- may be prepared by any methods well known in the art of pharmacy. The amount of active ingredient that can be combined with a carrier material to produce a single dosage form will generally be that amount of the compound that produces a therapeutic effect. Generally, out of one hundred per cent, this amount will range from about 1 per cent to about ninety-nine percent of active ingredient, preferably from about 5 per cent to about 70 per cent, most preferably from about 10 per cent to about 30 per cent.
Methods of preparing these formulations or compositions include the step of bringing into association a compound of the present invention with the carrier and, optionally, one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing into association a compound of the present invention with liquid carriers, or finely divided solid carriers, or both, and then, if necessary, shaping the product.
Formulations of the invention suitable for oral administration may be in the form of capsules, cachets, pills, tablets, lozenges (using a flavored basis, usually sucrose and acacia or tragacanth), powders, granules, or as a solution or a suspension in an aqueous or non-aqueous liquid, or as an oil-in- water or water-in-oil liquid emulsion, or as an elixir or syrup, or as pastilles (using an inert base, such as gelatin and glycerin, or sucrose and acacia) and/or as mouth washes and the like, each containing a predetermined amount of a compound of the present invention as an active ingredient. A compound of the present invention may also be administered as a bolus, electuary or paste.
In solid dosage forms of the invention for oral administration (capsules, tablets, pills, dragees, powders, granules and the like), the active ingredient is mixed with one or more pharmaceutically acceptable carriers, such as sodium citrate or dicalcium phosphate, and/or any of the following: fillers or extenders, such as starches, lactose, sucrose, glucose, mannitol, and/or silicic acid; binders, such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinyl pyrrolidone, sucrose and/or acacia; humectants, such as glycerol; disintegrating agents, such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate; solution retarding agents, such as paraffin; absorption accelerators, such as quaternary ammonium compounds; wetting agents, such as, for example, cetyl alcohol and glycerol monostearate; absorbents, such as kaolin and bentonite clay; lubricants, such a talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof; and coloring agents. In the case of capsules, tablets and pills, the pharmaceutical compositions may also comprise buffering agents. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugars, as well as high molecular weight polyethylene glycols and the like.
-101- A tablet may be made by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared using binder (for example, gelatin or hydroxypropylmethyl cellulose), lubricant, inert diluent, preservative, disintegrant (for example, sodium starch glycolate or cross-linked sodium carboxymethyl cellulose), surface-active or dispersing agent. Molded tablets may be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.
The tablets, and other solid dosage forms of the pharmaceutical compositions of the present invention, such as dragees, capsules, pills and granules, may optionally be scored or prepared with coatings and shells, such as enteric coatings and other coatings well known in the pharmaceutical-formulating art. They may also be formulated so as to provide slow or controlled release of the active ingredient therein using, for example, hydroxypropylmethyl cellulose in varying proportions to provide the desired release profile, other polymer matrices, liposomes and/or microspheres. They may be sterilized by, for example, filtration through a bacteria-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions that can be dissolved in sterile water, or some other sterile injectable medium immediately before use. These compositions may also optionally contain opacifying agents and may be of a composition that they release the active ingredient(s) only, or preferentially, in a certain portion of the gastrointestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. The active ingredient can also be in micro-encapsulated form, if appropriate, with one or more of the above-described excipients.
Liquid dosage forms for oral administration of the compounds of the invention include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active ingredient, the liquid dosage forms may contain inert diluent commonly used in the art, such as, for example, water or other solvents, solubilizing agents and mulsifiers, such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (in particular, cottonseed, groundnut, com, germ, olive, castor and sesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
Besides inert dilutents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, coloring, perfuming and preservative agents.
Suspensions, in addition to the active compounds, may contain suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide,
-102- bentonite, agar-agar and tragacanth, and mixtures thereof.
Formulations of the pharmaceutical compositions of the invention for rectal or vaginal administration may be presented as a suppository, which may be prepared by mixing one or more compounds of the invention with one or more suitable nonirritating excipients or carriers comprising, for example, cocoa butter, polyethylene glycol, a suppository wax or a salicylate, and which is solid at room temperature, but liquid at body temperature and, therefore, will melt in the rectum or vaginal cavity and release the active compound.
Formulations of the present invention which are suitable for vaginal . administration also include pessaries, tampons, creams, gels, pastes, foams or spray formulations containing such carriers as are known in the art to be appropriate. Dosage forms for the topical or transdermal administration of a compound of this invention include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches and inhalants. The active compound may be mixed under sterile conditions with a pharmaceutically acceptable carrier, and with any preservatives, buffers, or propellants that may be required.
The ointments, pastes, creams and gels may contain, in addition to an active compound of this invention, excipients, such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof.
Powders and sprays can contain, in addition to a compound of this invention, excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and poly amide powder, or mixtures of these substances. Sprays can additionally contain customary propellants, such as chlorofluorohydrocarbons and volatile unsubstituted hydrocarbons, such as butane and propane.
Transdermal patches have the added advantage of providing controlled delivery of a compound of the present invention to the body. Such dosage forms can be made by dissolving or dispersing the compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate of such flux can be controlled by either providing a rate controlling membrane or dispersing the active compound in a polymer matrix or gel.
Ophthalmic formulations, eye ointments, powders, solutions and the like, are also contemplated as being within the scope of this invention.
Pharmaceutical compositions of this invention suitable for parenteral administration comprise one or more compounds of the invention in combination with one or more pharmaceutically acceptable sterile isotonic aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, or sterile powders which may,be reconstituted into sterile injectable solutions or dispersions just prior to use, which may
-103- contain antioxidants, buffers, bacteriostats, solutes which render the formulation isotonic with the blood of the intended recipient or suspending or thickening agents.
Examples of suitable aqueous and nonaqueous carriers that may be employed in the pharmaceutical compositions of the invention include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), and suitable mixtures thereof, vegetable oils, such as olive oil, and injectable organic esters, such as ethyl oleate. Proper fluidity can be maintained, for example, by the use of coating materials, such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants. These compositions may also contain adjuvants such as preservatives, wetting agents, emulsifying agents and dispersing agents. Prevention of the action of microorganisms may be ensured by the inclusion of various antibacterial and antifungal agents, for example, paraben, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents that delay absorption such as aluminum monostearate and gelatin.
In some cases, in order to prolong the effect of a drug, it is desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material having poor water solubility. The rate of absorption of the drug then depends upon its rate of dissolution which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally-administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the subject compounds in biodegradable polymers such as polylactide-polyglycolide. Depending on the ratio of drug to polymer, and the nature of the particular polymer employed, the rate of drug release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly (anhydrides). Depot injectable formulations are also prepared by entrapping the drug in liposomes or microemulsions that are compatible with body tissue.
The preparations of the present invention may be given orally, parenterally, topically, or rectally. They are of course given by forms suitable for each administration route. For example, they are administered in tablets or capsule form, by injection, inhalation, eye lotion, ointment, suppository, etc. administration by injection, infusion or inhalation; topical by lotion or ointment; and rectal by suppositories. Oral administration is preferred.
-104- The phrases "parenteral administration" and "administered parenterally" as used herein means modes of administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal and intrasternal injection and infusion.
The phrases "systemic administration," "administered systemically," "peripheral administration" and "administered peripherally" as used herein mean the administration of a compound, drug or other material other than directly into the central nervous system, such that it enters the patient's system and, thus, is subject to metabolism and other like processes, for example, subcutaneous administration.
These compounds may be administered to humans and other animals for therapy by any suitable route of administration, including orally, nasally, as by, for example, a spray, rectally, intravaginally, parenterally, intracistemally and topically, as by powders, ointments or drops, including buccally and sublingually.
Regardless of the route of administration selected, the compounds of the present invention, which may be used in a suitable hydrated form, and/or the pharmaceutical compositions of the present invention, are formulated into ■ pharmaceutically acceptable dosage forms by conventional methods known to those of skill in the art.
Actual dosage levels of the active ingredients in the pharmaceutical compositions of this invention may be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient. The selected dosage level will depend upon a variety of factors including the activity of the particular compound of the present invention employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compound employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the medical arts.
A physician or veterinarian having ordinary skill in the art can readily determine and prescribe the effective amount of the pharmaceutical composition required. For example, the physician or veterinarian could start doses of the compounds of the invention employed in the pharmaceutical composition at levels lower than that required in order to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved.
-105- In general, a suitable daily dose of a compound of the invention will be that amount of the compound that is the lowest dose effective to produce a therapeutic effect. Such an effective dose will generally depend upon the factors described above. Generally, intravenous and subcutaneous doses of the compounds of this invention for a patient, when used for the indicated analgesic effects, will range from about 0.0001 to about 100 mg per kilogram of body weight per day, more preferably from about 0.01 to about 50 mg per kg per day, and still more preferably from about 1.0 to about 100 mg per kg per day. An effective amount is that amount treats an Pinl associated state.
If desired, the effective daily dose of the active compound may be administered as two, three, four, five, six or more sub-doses administered separately at appropriate intervals throughout the day, optionally, in unit dosage forms.
While it is possible for a compound of the present invention to be administered alone, it is preferable to administer the compound as a pharmaceutical composition.
EXEMPLIFICATION OF THE INVENTION:
The invention is further illustrated by the following examples, which should not be construed as further limiting. The animal models used throughout the Examples are accepted animal models and the demonstration of efficacy in these animal models is predictive of efficacy in humans.
Tumor Inhibition Assays Pinl -modulating compounds are potent antitumor agents. The anti-tumor activity of Pinl -modulating compounds against glioblastoma cells is comparable to 1,3- bis(2-chloroethyl)-l -nitrosourea (BCNU), one of the most potent clinical useful antitumor agents. Misra, et al. 1982. J. Am. Chem. Soc. 104: 4478-4479
In vitro anti-tumor activity of Pinl -modulating compounds can be assayed by measuring the ability of Pinl -modulating compounds to kill tumor cells. Examples of appropriate cells lines include: human lung (A549); resistant human lung with low topo II activity (A549-VP); murine melanoma (B 16); human colon tumor (HCT116); human colon tumor with elevated pl70 levels (HCTVM); human colon tumor with low topo II activity (HCTVP); P388 murine lymph leukemia cells; and human colon carcinoma cell line (Moser) under standard conditions. After the cells are cultured for twenty-four hours and allowed to attach to a plate (t'.e. a 96-well flat bottom plate), the cells are incubated for 72 hours with serially diluted concentrations of Pinl - modulating compounds. From this data, the concentration of the compound at which 50% of the cells are killed (IC50) is determined. Kelly, et al, U.S. Patent No. 5,166,208 and Pandey, et. al. 1981. J. Antibiot. 34(11):1389-401.
-106- In vivo anti-tumor activity of Pinl-modulating compounds can be assayed for by a reduction of tumor cells in mammals (i.e. mice) and a resulting increase in survival time compared to untreated tumor bearing mammals. For example, CDFi mice are injected interperitoneally with a suspension of P388 murine lymph leukemia cells, Ehrlich carcinoma cells, B 16 melanoma cells, or Meth-A fibrosarcoma cells or other appropriate tumor cell line. Some of the mice are treated intraperitoneally with a Pinl- modulating compounds. Other mice are treated with saline. The in vivo activity of the compound is determined in terms of the % T/C which is the ratio of the mean survival time of the treated group to the mean survival time of the saline treated group times 100. Yokoi, et al, U.S. Patent No. 4,584,377; Kelly, et al, U.S. Patent No. 5,166,208; Wamick-Pickle, etal. 1981. J Antibiot. 34(l l):1402-7; and Pandey, et. al. 1981. J. Antibiot. 34(11):1389-401
The in vivo anti-tumor activity of Pinl-modulating compounds can also be assayed as inhibitors against an ovarian tumor growing in a human tumor cloning system. Tebbe, et al. 1971 J. Am. Chem. Soc. 93:3793-3795.
The invention is further illustrated by the following examples, which should not be construed as further limiting.
Example 1 Cell Based Cytotoxicity Assay (CBCA) of Pinl Modulating Compounds
Mammalian cells were seeded in 96 well flat bottom microtiter plates at a density of 5,000 6000 cells per well on day 0 in 0.1 mL of an appropriate growth media. On Day 1, the wells were aspirated and 0.1 mL of fresh media was added. The cells were then treated with 0.01 mL of 1 Ox drug dilutions in 10% DMSO in media and incubated at 37° C in a humidified, 5% CO atmosphere. The assay contained eight drug concentrations in triplicate as well as a triplicate control where cells were treated with 0.01 mL of 10% DMSO in media. On Day 4, the cells were incubated with 0.02 mL of a colorimetric cell-viability assay solution (MTS) prepared from 20 parts (3-(4,5- dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (Promega) at 2.0 mg/mL in PBS and 1 part phenazine methosulfate (Sigma) at 0.92 mg/mL in PBS for 2-3 hours at 37 °C. Background wells were prepared by incubating 0.02 mL of the colorimetric cell-viability assay solution with 0.1 mL of media in parallel with the cell containing wells. The absorbance at 490 nm was then measured with an ELISA plate reader and the absorbance recorded for the background wells was averaged and the mean value was subtracted from the cell containing wells. Percent cell viabilities at each drug concentration were calculated by dividing the mean absorbance at 490 nm of the treated wells by the mean absorbance at 490 nm of the untreated wells.
-107- ED50 values (the effective dose required to for 50% viability) were calculated by plotting drug concentrations versus percent cell viability.
To count cells, suspended cells (0.02 mL) were diluted into 0.18 mL of 0.2% trypan blue solution in PBS. Approximately 0.015 mL of the suspension was added to a chamber of a Levy counting hemacytometer. The viable cells were counted in each of the four sets of 16 squares that are at the corners of the closely ruled lines. The total number of viable cells from the 64 squares were then multiplied by 0.025 to obtain the concentration of cells in the stock suspension. (Number of cells in the 64 wells) x (0.025) = lxlO6 cells/mL (original stock).
Example 2 Specificity Assay for Inhibition of Proline Isomerase by Pinl Modulating Compounds
The proline isomerase activity assay is based on the method described by Fisher et al (Biomed. Biochim. Acta, 1984, 43: 1101-1111). Specifically, the enzyme (112 ng) was preincubated with 72 mM substrate at 4 °C for 30 minutes in an 80 μL reaction volume containing 0.02 mg/μL BSA, 0.8 mM DTT, and 35 mM HEPES (pH 7.8). Proteolysis of the substrate was initiated by the addition of 80 μL of trypsin at 0.4 mg/mL in 35 mM HEPES (pH 7.8) and the release of p-nitroaniline was monitored every 10 seconds at 390 nm using a microplate reader (MRD/8V/DIAS, Dynex
Technologies). Inhibition studies were preformed by adding 5 μL of inhibitors added in the pre-incubation mix. Inhibitors were at 0.4 mg/mL in 10% DMSO.
Multiple activity-based assays at multiple dilutions, performed as described above, were used to generate the curve from which the IC50 was determined. Several IC50 results were obtained for compounds in Table 2 using this experimental protocol.
Example 3 Specificity Assay for Inhibition of Pinl by Pinl Modulating Compounds
The specificity of the Pinl inhibitor compounds of the invention can be determined by the protease-coupled PPIase assay developed by Fischer et al. (Biomed. Biochim. Acta, 1984, 43: 1101-1111). For example, the enzyme activity of Pinl can be compared to members of the other known classes of PPIases, cyclophilins (e.g., hCyplδ, hCyP-A, hCyP-B, hCyP-C, and NKCA) and FKBPs (e.g., hFKBP12, hFKBP-12, hFKBP-13, and hFKBP-25) in the presence and absence of the compound.
In one assay, hPinl activity measurements are determined using bovine trypsin (final concentration 0.21 mg/mL, Sigma) as an isomer specific protease and
-108- Ac-Ala-Ala-Ser(P)-Pro-Arg-pNA (Jerini, Germany) as a substrate. PPIase activity of hFKBP12 (Sigma) and hCyplδ (Sigma) is determined with the peptide substrate Suc-Ala-Phe-Pro-Phe-pNA (Bachem) and the protease α-chymotrypsin (final concentration 0.41 mg/mL, Sigma). The test can be performed by observing the released 4-nitroanilide at 390 nm with a Hewlett-Packard 8453 UN-vis spectrophotometer at 10°C. The total reaction volume is adjusted to 1.23 mL by mixing appropriate volumes of 35 mM HEPES (pH 7.8) with enzyme and effector solutions. The Pinl inhibitor compound is freshly diluted from a 1 mg/mL stock solution in DMSO, and pre-incubated at varying concentrations with the enzyme for 5 min (10°C). Prior to the start of reaction by addition of the respective protease, 2 μL of the peptide substrate stock solution (10 mg/mL in DMSO) is added. The amount of organic solvent is kept constant within each experiment (< 0.1%). The pseudo-first-order rate constant k0bs for cis/trans isomerization in the presence of PPIase and the first-order rate constant k0 of the uncatalyzed cis/trans isomerization can be calculated using the Kinetics Software of Hewlett-Packard as well as SigmaPlot2000 for Windows 6.0 (SPSS).
The K; value for inhibition of Pinl PPIase activity by a Pinl inhibitor compound of the invention at constant concentrations of substrate ([S0]«KM) can then be calculated by fitting the data according to the equation for a competitive
"tight-binding" inhibitor using SigmaPlot2000
Example 4
Cellular Screen Secondary Cell Based Activity Assay (Determination of ED so)
A cell solution is added to a flask containing containing 13 ml of 10% FBS with EDTA. The cell suspension is centrifuged at 1500g for 5 minutes and resuspend in 10 mL media. The centriguge procedure is repeated. The cells are resuspended in 2 mL of media. 20 μl of cell suspension is added to 180 μL 0.2% trypahn blue. Approximately 2000 cells are added to each well of a micortitre plate in 100 μL media. After cells have grown for an appropriate time (~l-2 days depending on cell line) 10 μL of a stock solution containing a test compound is added to each well. After further growth, the media is removed from the well and tyrpsin is added. After a short incubation, the trypsin is removed or inactivated and the cells are counted using a Guava Cell Analysis System (Hayward, CA).
-109- Example 5 Method for Evaluating Pinl Levels
In one embodiment, the automated cellular imaging system (ACIS) was used to determine tissues with elevated Pinl Levels. The data that is presented in Example 4 is from U.S. Patent Application No. 10/071,747, filed February 8, 2002, the entire contents of which are incorporated by reference.
Micro-histoarray sections were scanned and images were captured using the automated cellular imaging system (ChromaNision Medical Systems, Inc., San Juan Capistrano, CA), which combines automated microscopy and computerized image processing to analyze multiple tissues on a single slide. ACIS was used to analyze microarray tissue sections on glass slides stained using a diaminodenzidine chromagen (DAB) and hematoxylin counterstain. Positive staining (brown color) as viewed by light microscope indicates the presence of the protein, and color intensity correlates directly with protein quantity (expression). The ACIS was able to recognize 255 levels of immnohistochemical staining intensity (0-255) and converted these to fractional scores for the selected individual areas. However, the base limit on the threshold for the Generic DAB is pre-set at 50 by the manufacturer because the system is very sensitive. Therefore, any intensity below 50 was treated as 0 in this study. Entire immunostahied tissue sections were scanned using the 4 X objective and images were captured using the 10X objective.
Calculation of Pin protein expression in human cancers:
In this study, intensity scoring and the percent positive scoring (brown area was divided by total area) were used with the entire individual tissue dot selected. The immunohistochemical staining was quantitated without knowledge of a pathologist' s score. All tissue samples were immunostained twice at one location, e.g., the University of Basel and confirmed at a second location, e.g., Pintex Pharmaceuticals, Inc., followed by an evaluation of the two data sets, e.g., at Pintex Pharmaceuticals, Inc. For example, the final score was obtained by using the average of the two data sets and was calculated by the formulation:
score = intensity + (X percent positive staining).
-110- The % of total cases showing elevated levels (over-expression) of Pin 1 = [numbers of tumor samples with score larger than the score of the highest normal easel total number of tumor samples multiplied by 100.
Results:
Table 8
Pinl protein over-expression in human tissues microarray
Figure imgf000113_0001
-111-
Figure imgf000114_0001
-112-
Figure imgf000115_0001
-113- Example 6 Synthetic Methods of Preparation of Compounds of the Invention
General Experimental Conditions
Liquid chromatography data was obtained using an Hewlett-Packard
(HP) 1100 Series Liquid Chromatograph coupled to a Diode Array Detector [ Zorbax
Eclipse XDB-C8 column; particle size 5μm, 150 mm column length, 4.6m column diameter; flow rate of ImL/min; Solvent program, from 95% H2O (w/ 0.1 % TFA)/ 5% Acetonitrile ( w/0.1% TFA ) to 100% Acetonitrile in 18 minutes, then held constant for 2 minutes; detection wavelength 254 nm]. Mass spectrometric data was obtained using a HP 6980 Gas Chromatograph coupled to a 5973 Mass Selective Detector: Agilent HP1 column, 15 m column length, 0.25 mm column diameter, 0.1 μm column film, 280°C injector temperature, initial oven temperature of 200°C for 3 minutes, changed to 325°C over 5 minutes, and held constant for 6 minutes. Thin-layer chromatography was performed on EM Science MK6F silica gel glass TLC plates and UN light was used for detecting compounds on the TLC plates. Reagents used in reactions were purchased from Aldrich Chemical Company (Milwaukee, MO), Sigma Chemical Company (Milwaukee, MO), Fluka Chemical Company (Milwaukee, MO), Fisher Scientific (Pittsburg, PA), TCI America (Portland, OR), Ryan Scientific (Isle of Palms, SC), Lancaster Synthesis (Windham, ΝH ), Asinex (Moscow, Russia), Chembridge Corporation (San Diego, CA), Matrix Scientific (Columbia, SC) or Oakwood Products Inc. (West Columbia, SC).
Three synthetic routes have been used to prepare rhodanine analogs described in this invention.
-114- General description of synthetic schemes
Method A
Figure imgf000117_0001
Method B h? Λ
Method C h* A0
Figure imgf000117_0002
Figure imgf000117_0003
Method A condenses an aldehyde with the active methylene of an appropriately substituted rhodanine carboxylic acid under Aldol conditions. Method B condenses the aldehydes with the corresponding ester-protected carboxylic acid, followed by hydrolytic cleavage of the ester to provide the carboxylic acid. Method C demonstrates that the Aldol condensation can be carried out using polymer-bound rhodanine. The final product can subsequently be released from the polymer support under mild conditions to provide the analogs of the invention.
General Procedure for Suzuki Cross Coupling
Figure imgf000117_0004
II.
0.805g (1.1 eq.; 6.6 mmol) Phenylboronic acid (I.)
1.05 g (1 eq.; 6 mmol) 5-Bromo-2-furaldehyde (II.)
0.2g Tetrakis(triphenylphosphine)palladium(0) (III.)
1.59 g (2.5 eq.; 15 mmol) Na2CO3
10 mL distilled water
25 mL Ethylene glycol dimethyl ether (Peroxide free!)
-115- Reaction: 5-bromo-2-furaldehyde (II.) was dissolved in ethylene glycol dimethyl ether (25 mL) under nitrogen (inert atmosphere) then Pd(PPh3)4 catalyst was added and the mixture was stirred for 10 minutes. Then phenylboronic acid (I.) and the solution of Na2CO3 in 10 mL distilled water was added and the reaction mixture refluxed for min.4 hours. The reaction was monitored by TLC (on silica, eluent: hexane : ethylacetate = 3 :2).
Work up: 30 mL distilled water was added to the reaction mixture, then it was extracted with ethylacetate 3 times. The organic phase was dried over MgSO4 then evaporated.
Purification: The crude product was purified by liquid chromatography (on silica, eluent: chloroform). After evaporation the crystalline product was washed with diethylether.
Yield: around 70 %. (Stored under inert atmosphere at 0-5 °C).
General Procedure for the Preparation of the Pd(0) catalyst
DMSO PdCI2 + P(Ph)3 + H2N-NH2 * H2O ^ Pd(PPh3)4
III.
1.77 g PdCl2 (I.) 13.1 g Triphenylphosphine (II.) 2 mL Hydrazine monohydrate (III.) 120 mL Dimethyl sulfoxide
I. and II. were added to DMSO under nitrogen at room temperature. The mixture was heated using a hot oil bath at 140 °C and kept at this temperature until the mixture became a clear solution (around 15-30 minutes). Then III. was added dropwise (in 1 min.) to the vigorously stirred reaction mixture. After addition of III. to the reaction mixture, the reaction was cooled immediately to room temperature with a water bath. The precipitate was filtered off and washed quickly 3 times with 5 mL of cool ethanol then 4 times with 5 mL diethylether. (The reaction was kept under a blanket of nitrogen during the entire course of the above reaction.)
-116- Reference: Coulson, Inorg. Synth. 1972 (13) 121
Preparation of Rhodanine Derivatives:
(1) C-6 Carboxylic Acid Derivative (Intermediate for Method A)
Figure imgf000119_0001
The synthesis of this material followed established literature procedures. See JOC, USSR, Engl. Transl. 2, 1315 (1966). A representative example follows;
To 10.0 g aminocaporic acid ( 76.2 mmol) in 34.1 mL 22% KOH (~ 1.75 eq.) was added dropwise CS2 (5.0 mL, ~ 1.1 eq.), making sure the temperature of the reaction did not exceed 25 °C. The yellow mixture was allowed to mix at room temperature for approximately 3 hours, at which time 10.6 g (1.0 eq.) of bromoacetic acid was added as a solid in small portions over about 20 minutes. The reaction was allowed to mix at room temperature for an additional 3 hours, during which time a precipitate formed. The reaction pH was adjusted to 3 - 4 using concentrated sulfuric acid, and allowed to stand overnight at room temperature. The yellow/orange material was filtered and washed with water. Recrystallization was accomplished using 1 : 1 EtOH:H2O. Isolated ~ 11.5 g of material (~ 61%)
(2) Synthesis of Final Product Using Method A
Figure imgf000119_0002
The synthesis of this material followed the procedure provided by Pintex with minor modifications, as follows;
-117- In ~ 20 mL of toluene was added 1.0 g (~ 4 mmol) of the rhodanine-C-6 carboxylic acid and 970 mg (1 eq.) of the furaldehyde. A layer of molecular sieves was added and ~ 15mg of pyridinium tosylate and the reaction was brought to gentle reflux for about 3 - 4 hours. The reaction was monitored by TLC, to determine when consumption of starting materials was complete. The reaction was filtered hot and washed with hot toluene and hot ethanol. The mother liquor was evaporated to dryness.
The crude product was dissolved in ~ 500 mL of acetone and warmed on a hot plate, filtered warm and washed with warm acetone. The clear yellow/orange solution was placed back on a hot plate and brought to a gentle reflux. After - 50 mL of acetone had evaporated, - 50 mL of water was added and the solution allowed to continue gentle refluxing until an additional - 50 mL of acetone had evaporated. An additional 50 mL of water was added and the solution was allowed to continue refluxing until it became cloudy, at which point is was removed from the hot plate, washed down the sides with acetone, allowed to cool to room temperature, and then placed overnight in a refrigerator.
The crystals were filtered, washed with cold acetone (~ 100 mL) and water. A bright orange material isolated, - 930 mg (~ 49%). LC suggested a trace contaminant and the material was re-recrystalized as above. Isolated - 840 mg from the second recrystalization.
To make the Na salt, - 435 mg of the free acid was dissolved in - 500 mL of acetone and 77 mg (-1 eq.) of NaHCO3 in - 1 mL of water was added. The mixture was allowed to mix for about 5 min. and then concentrated on a rotary evaporator. The material was triturated with ether/acetone, filtered and washed with ether to yield - 350 mg of an orange material.
B.
(1) C-4 Ester Derivative (Intermediate for Method B)
Figure imgf000120_0001
4.55 g (26.65 mmol) II.
-118- 2.73 mL (3.0g; 25 mmol) I.
875 microL (625 mg; 7.5 mmol) ttiethylamine
50 mL 1,2-dichloroethane (HPLC purity)
Reaction: I. and II. were dissolved in 1 ,2-dichloroethane and TEA was added. The reaction mixture was stirred for 1 hour at r.t.
Work up: The solvent was removed in vacuo, to the residue isopropanol was added (25 mL) and the mixture cooled to 0 oC, the precipitated product was filtered off and washed two times with 5 mL cooled isopropanol.
TLC: silica/dichloroethane : ethanol = 10 : 1.
Product: 5.5 g (Yield=89%)
(2) Synthesis of Final Product Using Method B
Figure imgf000121_0001
R2 = 5-(4-nitrophenyl)furanyi-
1) Alky lation of rhodanine
Rhodanine (MW=133.19) n=50 mmol, m=6.66 g ethyl-4-bromobutyrate (MW=195.06) 1.1 eq, 55 mmol, V=7.9 mL
NaH 1.5 eq, 75 mmol, m=2.9 g (60% suspension in THF)
To the cooled (-10 0 °C) THF, NaH was added dropwise under argon. After 10 min. stirring, rhodanine was added, and after the another 15 min. ethyl-4- bromobutyrate was added dropwise. The mixture was stirred under reflux for about 1.5-
-119- 2 h (monitored by TLC), and left to cool down. The NH jCl was added and THF was evaporated. The residue was dissolved in ethyl acetate and extracted with brine. After drying (MgSO ) the crude product was used to the further reaction.
2) Condensation of rhodanine with an aldehyde alkylated rhodanine (MW=247) n=5 mmol, m=1.24 g 5-(4-nitrophenyl)-furfural (MW=251.09) 1 eq, m=l .26g piperidine 0.03 eq, 0.15 mmol, V=0.015 mL
AcOH 0.03 eq, 0.15 mmol, V=0.008 mL A mixture of all the compounds in toluene ( 100 cm3) was heated under reflux with azeotropic remowal of water for about 5 h. The mixture was cooled to 5 °C, filtration gave crude product which was used to the further synthesis.
3) Hydrolysis of ethyl ester The product of the previous reaction (MW=480, n=3 mmol) was dissolved in MeOH, and IN NaOH was added (1.1 eq). The mixture was stirred at room temperature for about 6 h. After that time the mixture was acidified with IN HCl to the pH 6 and MeOH was evaporated. The residue was acidified to the pH 2 and extracted with ethyl acetate (4 times). After drying (MgSO ) the crude product was crystallised (MeOH/ Et2O).
(1) Synthesis of Compounds on Solid Support.
1) Coupling of Fmoc-γ-Abu-OH with Wang resin (symmetrical anhydride coupling).
2) Synthesis of rhodanine on solid support To 1 g Fmoc-γ- Abu- Wang resin were added 20% piperidine in DMF and the mixture was shaken for 30 min. The resin was washed (DMF, MeOH and CH2C12) and dried.
A mixture of resin, thiocarbonyldiimidazole (5 mol eq) and TEA (3 mol eq) in CH2C12 was shaken for 1 h. The filtrate was drained away and the resin was swollen in CH C12 and methyl thioglycolate (5 mol eq) was added. The reaction mixture was further shaken for about 16 h. The resin was washed (DMF, MeOH and CH2C12) and dried.
-120- 3) Condensation of aldehydes with rhodanine (and subsequent cleavage of the product from the resin)
The loaded resin and an aldehyde (5 mol eq) was heated in toluene for about 6 h. The resin was washed (DMF, MeOH and CH2C12), resuspended in 20% TFA/ CH2CI2 and shaken for 1 h. The filtrate was pooled and concentrated to yield an exact product (Yield = 80-90%).
EQUIVALENTS Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments and methods described herein. Such equivalents are intended to be encompassed by the scope of the following claims.
INCORPORATION BY REFERENCE
The entire contents of all patents, published patent applications and other references cited herein are hereby expressly incorporated herein in their entireties by reference.
-121-

Claims

1. A method for treating a Pinl -associated state in a subj ect comprising administering to said subject an effective amount of a Pinl-modulating compound of formula (I):
Figure imgf000124_0001
ω wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1;
Z and Zi are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2O-, -S-, or - OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino-l,3-dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide; such that said Pinl-associated state is treated.
-122-
2. The method of claim 1 , wherein Z is S .
I
3. The method of claim 1 , wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3-(methylene)-l-methyl-l,3-dihydro-indol-2-one, abenzo[l,3]dioxole, and Furazan 2-oxide.
4. The method of claim 1, wherein n is selected from the group consisting of 0 through 5.
5. The method of claim 1 , wherein Zi is O.
6. The method of claim 1 , wherein the Pinl-modulating compound of formula (I) is a compound of formula (II):
Figure imgf000125_0001
(ID wherein R2, R3, and Re are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R4 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of -CH and N;
Ri is -(CH2)n-(X4) -(CH2) -Cθ2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1 ; wherein each CH2 group may be
-123- independently substituted with - alkyl, e.g., CH3, CH2CH2SCH3, or OH.
7. The method of claim 1, wherein the Pinl-modulating compound of formula (I) is a compound of formula (III):
Figure imgf000126_0001
(III) wherein
R , R3, and F are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, C02H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4;
R5 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of -CH and N; R] is -(CH2)n-( 4)P-(CH2)m-Cθ2R6, wherein R6 is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Cι-C6 alkyl, e.g., CH3, OH, or CH2CH2SCH3.
8. The method of claim 1 , wherein said Pin 1 -modulating compound is a
Pinl -inhibiting compound.
9. The method of claim 1 or 8, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
-124-
10. The method of claim 1 or 8, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
11. The method of claim 1 or 8, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
12. The method of claim 1 or 8, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
13. The method of claim 1 or 8, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
14. The method of claim 1 or 8, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
15. The method of claim 1 or 8, wherein said Pinl-associated state is a cyclin Dl elevated state.
16. The method of claim 1 or 8, wherein said Pinl-associated state is neoplastic transformation.
17. The method of claim 1 or 8, wherein said Pinl-associated state is cancer.
18. The method of claim 1 or 8, wherein said Pinl-associated state is tumor growth.
19. The method of claim 1 or 8, wherein said method of treating said Pinl - associated state comprises inhibiting tumor growth.
20. The method of claim 1 or 8, wherein said method of treating said Pinl- associated state comprises preventing the occurrence of tumor growth in the subject.
21. The method of claim 1 or 8, wherein said method of treating said Pinl - associated state comprises reducing the growth of a pre-existing tumor in the subject.
22. The method of claim 1 or 8, wherein said Pinl-associated state is colon cancer or breast cancer.
-125-
23. The method of claim 1 or 8, wherein said Pinl-associated state is sarcoma or a malignant lymphoma.
24. The method of claim 1 or 8, wherein said Pinl-associated state is esophageal cancer, oligodendroglioma, astrocytoma, glioblastomamultiforme, cervical carcinoma, ovary endometroid cancer, ovary Brenner tumor, ovary mucinous cancer, ovary serous cancer, uterus carcinosarcoma, breast lobular cancer, breast ductal cancer, breast medullary cancer, breast mucinous cancer, breast tubular cancer, thyroid adenocarcinoma, or thyroid follicular cancer.
25. The method of claim 1 or 8, wherein said Pinl-associated state is thyroid medullary cancer, thyroid papillary carcinoma, parathyroid adenocarcinoma, adrenal gland adenoma, adrenal gland cancer, pheochromocytoma, colon adenoma mild displasia, colon adenoma moderate displasia, colon adenoma severe displasia, or colon adenocarcinoma.
26. The method of claim 1 or 8, wherein said Pinl-associated state is esophagus adenocarcinoma, hepatocelluar carcinoma, mouth cancer, gall bladder adenocarcinoma, pancreatic adenocarcinoma, prostate, prostate cancer, testis non- seminomatous cancer, testis seminoma, urinary bladder transitional carcinoma, lung adenocarcinoma, lung large cell cancer, lung small cell cancer, lung squamous cell carcinoma, MALT lymphoma, NHL diffuse large B, non-Hodgkin's lymphoma (NHL), thymoma, skin malignant melanoma, skin basolioma, skin squamous cell cancer, skin merkel zell cancer, skin benign nevus, lipoma, endometriod carcinoma, endometrium serous carcenoma, small intestine adenocarcinoma, stomach diffuse adenocarcinoma, kidney chromophobic carcinoma, kidney clear cell carcinoma, kidney oncocytoma, kidney papillary carcinoma, Hodgkin lymphoma or liposarcoma.
27. The method of claim 1 , wherein said Pinl -associated state is associated with the overexpression of Pinl and/or DNA damage.
28. The method of claim 1 , wherein said Pinl -associated state is associated with an oncogenic protein.
29. The method of claim 1 , wherein said Pinl -associated state is associated with Ha-Ras.
-126-
30. The method of claim 1 , wherein said Pinl -modulating compound has a characteristic inhibition profile (CIP) and has a cytotoxicity effective to treat said Pinl- associated state.
31. The method of claim 30, wherein said Pinl -modulating compound has an
IC50 value of less than about 40.
32. The method of claim 31 , wherein said IC50 value of between about 10 and about 40.
33. The method of claim 31 , wherein said IC50 value of between about 1 and about 10.
34. The method of claim 31 , wherein said IC50 value of less than about 1.
35. The method of claim 30, wherein said Pinl-modulating compound has a cytotoxicity of about 3 μM or less as measured by the CBCA.
36. The method of claim 35, wherein said Pinl-modulating compound has a cytotoxicity of about 1.5 μM or less as measured by the CBCA.
37. The method of claim 36, wherein said Pinl-modulating compound has a cytotoxicity of about 1 μM or less as measured by the CBCA.
-127-
38. A method for treating cyclin Dl overexpression in a subject comprising administering to said subject an effective amount of a Pinl-modulating compound of formula (I):
Figure imgf000130_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1;
Z and Z\ are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked, or indirectly linked by alkylene, -S(O) O-, -S-, or - OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof; Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino-l,3-dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide; such that said cyclin Dl overexpression is treated.
39. The method of claim 38 , wherein Z is S .
-128-
40. The method of claim 38, wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3-(methylene)-l-methyl-l,3-dihydro-indol-2-one, abenzo[l,3]dioxole, and Furazan 2-oxide.
41. The method of claim 38, wherein n is selected from the group consisting of 0 through 5.
42. The method of claim 38, wherein
Figure imgf000131_0001
is O.
43. The method of claim 38, wherein the Pinl -modulating compound of formula (I) is a compound of formula (II):
Figure imgf000131_0002
(ID wherein
R2, R3, and R^ are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R4 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of -CH and N;
Ri is -(CH2)„-(X4)p-(CH2)m-CO2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; 4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with CrC6 alkyl, e.g., CH3, CH2CH2SCH3, or OH.
-129-
44. The method of claim 38, wherein the Pinl -modulating compound of formula (I) is a compound of formula (III):
Figure imgf000132_0001
(III) wherein R2, R3, and 1^ are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R5 is H or lower alkyl, e.g., CH3; Xi, 2 an X3 are independently selected from the group consisting of-CH and ; Ri is -(CH2)n-( )p-(CH2)πrCθ2R6, wherein R6 is H or lower alkyl, e.g., t-butyl or CH2CH3; X* is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with CrC6 alkyl, e.g., CH3, OH, or CH CH2SCH3.
45. The method of claim 38, wherein the cyclin Dl overexpression results in neoplastic transformation.
46. The method of claim 38, wherein the cyclin Dl overexpression results in tumor growth.
47. The method of claim 38, wherein said method for treating cyclin D 1 overexpression comprises inhibiting tumor growth.
-130-
48. The method of claim 38, wherein said method for treating cyclin Dl overexpression comprises preventing the occurrence of tumor growth in the subject.
49. The method of claim 38, wherein said method for treating cyclin Dl overexpression comprises reducing the growth of a pre-existing tumor in the subject.
50. The method of claim 38, wherein the cyclin Dl overexpression results in colon cancer or breast cancer.
51. The method of claim 38, wherein the cyclin D 1 overexpression results in a sarcoma or a malignant lymphoma.
52. The method of claim 38, wherein the cyclin Dl overexpression results in esophageal cancer, oligodendroglioma, astrocytoma, glioblastomamultiforme, cervical carcinoma, ovary endometroid cancer, ovary Brenner tumor, ovary mucinous cancer, ovary serous cancer, uterus carcinosarcoma, breast lobular cancer, breast ductal cancer, breast medullary cancer, breast mucinous cancer, breast tubular cancer, thyroid adenocarcinoma, or thyroid follicular cancer.
53. The method of claim 38, wherein the cyclin D 1 overexpression results in thyroid medullary cancer, thyroid papillary carcinoma, parathyroid adenocarcinoma, adrenal gland adenoma, adrenal gland cancer, pheochromocytoma, colon adenoma mild displasia, colon adenoma moderate displasia, colon adenoma severe displasia, or colon adenocarcinoma.
54. The method of claim 38, wherein the cyclin D 1 overexpression results in esophagus adenocarcinoma, hepatocelluar carcinoma, mouth cancer, gall bladder adenocarcinoma, pancreatic adenocarcinoma, prostate, prostate cancer, testis non- seminomatous cancer, testis seminoma, urinary bladder transitional carcinoma, lung adenocarcinoma, lung large cell cancer, lung small cell cancer, lung squamous cell carcinoma, MALT lymphoma, NHL diffuse large B, non-Hodgkin's lymphoma (NHL), thymoma, skin malignant melanoma, skin basolioma, skin squamous cell cancer, skin merkel zell cancer, skin benign nevus, lipoma, endometriod carcinoma, endometrium serous carcenoma, small intestine adenocarcinoma, stomach diffuse adenocarcinoma, kidney chromophobic carcinoma, kidney clear cell carcinoma, kidney oncocytoma, kidney papillary carcinoma, Hodgkin lymphoma, or a liposarcoma.
-131-
55. The method of any one of claims 38-54, wherein the cyclin Dl overexpression is caused by overexpression of Pinl.
56. The method of any one of claims 38-54, wherein the cyclin Dl overexpression is caused by DNA damage.
57. The method of any one of claims 38-54, wherein the cyclin D 1 overexpression is caused by an oncogenic protein.
58. The method of any one of claims 38-54, wherein cyclin D 1 overexpression is caused by Ha-Ras.
59. The method of any one of claims 38-58, wherein said Pinl modulating compound is a Pinl inhibiting compound.
60. The method of any one of claims 38-58, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
61. The method of any one of claims 38-58, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
62. The method of any one of claims m 38-58, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
63. The method of any one of claims 38-58, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
64. The method of any one of claims 38-58, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
65. The method of any one of claims 38-58, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives
-132- thereof.
66. The method of claim 38, wherein said Pinl-modulating compound has a characteristic inhibition profile (CIP) and has a cytotoxicity effective to treat said Pinl- associated state.
67. The method of claim 66, wherein said Pinl -modulating compound has an IC5Q value of less than about 40.
68. The method of claim 67, wherein said 1C50 value of between about 10 and about 40.
69. The method of claim 67, wherein said IC50 value of between about 1 and about 10.
70. The method of claim 67, wherein said IC50 value of less than about 1.
71. The method of claim 66, wherein said Pinl -modulating compound has a . cytotoxicity of about 3 μM or less as measured by the CBCA.
72. The method of claim 71 , wherein said Pinl-modulating compound has a cytotoxicity of about 1.5 μM or less as measured by the CBCA.
73. The method of claim 72, wherein said Pinl -modulating compound has a ' cytotoxicity of about 1 μM or less as measured by the CBCA.
-133-
74. A packaged Pinl -associated state treatment, comprising a Pinl - modulating compound of formula (I):
Figure imgf000136_0001
WAm ]n
AR
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is 0 or 1 ;
Z and Z\ are independently selected from the group consisting of
O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ-, -S-, or - OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
Rt is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- benzenesulfona ide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino-l,3-dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide; packaged with instructions for using an effective amount of the Pinl-modulating compound to treat a Pinl-associated state.
75. The packaged Pinl-associated state treatment of claim 74, wherein Z is S.
-134-
76. The packaged Pinl-associated state treatment of claim 74, wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3 -(methylene)-l -methyl- 1,3 -dihydro- indol-2-one, a benzo[l,3]dioxole, and Furazan 2-oxide.
77. The packaged Pinl-associated state treatment of claim 74, wherein n is selected from the group consisting of 0 through 5.
78. The packaged Pinl-associated state treatment of claim 74, wherein Z\ is O.
79. The packaged Pinl -associated state treatment of claim 74, wherein the
Pinl-modulating compound of formula (I) is a compound of formula (II):
Figure imgf000137_0001
(II) wherein
R2, R3, and R<s are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, -
CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R4 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of -CH and N;
Ri is -(CH2)n-(X )P-(CH2)m-CO2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1 ; wherein each CH2 group may be independently substituted with d-C6 alkyl, e.g. , CH3, CH2CH2SCH3, or OH.
-135-
80. The packaged Pinl-associated state treatment of claim 74, wherein the
Pinl-modulating compound of formula (I) is a compound of formula (III):
Figure imgf000138_0001
(III) wherein
R2, R3, and R4 are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R5 is H or lower alkyl, e.g. , CH3;
Xi, X2 and X3 are independently selected from the group consisting of -CH and N; Ri is -(CH2)n-(X4)p-(CH2)m-C02R6, wherein Re is H or lower alkyl, e.g., t-butyl or CH2CH3; 4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Cι-C6 alkyl, e.g., CH3, OH, or CH2CH2SCH3.
81. The packaged Pinl-associated state treatment of claim 74, wherein said
Pinl modulating compound is a Pinl inhibiting compound.
82. The packaged Pinl-associated state treatment of claim 74, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
-136-
83. The packaged Pinl-associated state treatment of claim 74, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
84. The packaged Pinl-associated state treatment of claim 74, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
85. The packaged Pinl-associated state treatment of claim 74, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
86. The packaged Pinl -associated state treatment of claim 74, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
87. The packaged Pinl-associated state treatment of claim 74, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
88. A packaged cyclin D 1 overexpression treatment, comprising a Pin 1 - modulating compound of formula (I):
Figure imgf000139_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10;
-137- m is O or l;
Z and Z\ are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ-, -S-, or - OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof; . Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino-l,3-dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide ; packaged with instructions for using an effective amount of the Pinl-modulating compound to treat cyclin Dl overexpression.
89. The packaged cyclin Dl overexpression treatment Pinl-associated state treatment of claim 88, wherein Z is S.
90. The packaged cyclin Dl overexpression treatment Pinl-associated state treatment of claim 88, wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3- (methylene)-l-methyl-l,3-dihydro-indol-2-one, abenzo[l,3]dioxole, and Furazan 2- oxide.
91. The packaged cyclin D 1 overexpression treatment Pinl -associated state treatment of claim 88, wherein n is selected from the group consisting of 0 through 5.
92. The packaged cyclin Dl overexpression treatment Pinl-associated state treatment of claim 88, wherein Zi is O.
-138-
93. The packaged cyclin D 1 overexpression treatment Pinl -associated state treatment of claim 88, wherein the Pinl-modulating compound of formula (I) is a compound of formula (II):
Figure imgf000141_0001
(II) wherein
R2, R3, and Re are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R4 is H or lower alkyl, e.g. , CH3; Xi, X2 and X3 are independently selected from the group consisting of -CH and N;
Ri is -(CH2)n- )P-(CH2)n,-Cθ2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Ci-C6 alkyl, e.g., CH3, CH2CH2SCH3, or OH.
-139-
94. The packaged cyclin Dl overexpression treatment Pinl-associated state treatment of claim 88, wherein the Pinl-modulating compound of formula (I) is a compound of formula (III):
Figure imgf000142_0001
(III) wherein
H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, -CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4;
R5 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of-CH andN; Ri is -(CH2)n-p 4)p-(CH2)m-C02R6, wherein Rg is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Ci-Ce alkyl, e.g., CH3, OH, or CH2CH2SCH3.
-140-
95. The packaged cyclin D 1 overexpression treatment Pinl-associated state treatment of claim 88, wherein said Pinl modulating compound is a Pinl inhibiting compound.
96. The packaged cyclin D 1 overexpression treatment Pinl -associated state treatment of claim 88, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
97. The packaged cyclin Dl overexpression treatment Pinl-associated state treatment of claim 88, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
98. The packaged cyclin Dl overexpression treatment Pinl-associated state treatment of claim 88, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
99. The packaged cyclin Dl overexpression treatment Pinl-associated state treatment of claim 88, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
100. The packaged cyclin Dl overexpression treatment Pinl-associated state treatment of claim 88, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
101. The packaged cyclin D 1 overexpression treatment Piril -associated state treatment of claim 88, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
-141-
102. A packaged cancer treatment, comprising a Pinl -modulating compound of formula (I):
Figure imgf000144_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is 0 or 1 ;
Z and Zi are independently selected from the group consisting of
O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ-, -S-, or - OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- . benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino-l,3-dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide; packaged with instructions for using an effective amount of the Pinl-modulating compound to treat cancer.
103. The packaged cancer treatment of claim 102, wherein Z is S.
-142-
104. The packaged cancer treatment of claim 102, wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3-(methylene)-l-methyl-l,3-dihydro-indol-2-one, a benzo[l,3]dioxole, and Furazan 2-oxide.
105. The packaged cancer treatment of claim 102, wherein n is selected from the group consisting of 0 through 5.
106. The packaged cancer treatment of claim 102, wherein Zi is O.
107. The packaged cancer treatment of claim 102, wherein the Pinl - modulating compound of formula (I) is a compound of formula (II):
Figure imgf000145_0001
(II) wherein
R2, R3, and e are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; is H or lower alkyl, e.g. , CH3; Xi, X2 and X3 are independently selected from the group consisting of -CH and N;
Ri is -(CH2)n-(X4)p-(CH2)m-CO2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; 4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Cι-C6 alkyl, e.g., CH3, CH2CH2SCH3, or OH.
-143-
108. The packaged cancer treatment of claim 102, wherein the Pinl- modulating compound of formula (I) is a compound of formula (III):
Figure imgf000146_0001
(HI) wherein
R2, R3, and R4 are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R5 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of -CH and N; Ri is -(CH2)n-( 4)P-(CH2)m-Cθ2Re, wherein Re is H or lower alkyl, e.g., t-butyl or CH2CH3; Xi is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Cι-C6 alkyl, e.g., CH3, OH, or CH2CH2SCH3.
-144-
109. The packaged cancer treatment of claim 102, wherein said Pinl modulating compound is a Pinl inhibiting compound.
110. The packaged cancer treatment of claim 102, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
111. The packaged cancer treatment of claim 102, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
112. The packaged cancer treatment of claim 102, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
113. The packaged cancer treatment of claim 102, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
114. The packaged cancer treatment of claim 102, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
115. The packaged cancer treatment of claim 102, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
-145-
116. A method for treating a Pinl-associated state in a subject comprising administering to a subject an effective amount of a combination of a Pinl-modulating compound of formula (I):
Figure imgf000148_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1;
Z and Zi are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ-, -S-, or - OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof; Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino-l,3-dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide; and a hyperplastic inhibitory agent such that the Pinl-associated state is treated.
-146-
117. The method of claim 116, wherein Z is S.
118. The method of claim 116, wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3-(methylene)-l-methyl-l,3-dihydro-indol-2-one, a benzo[l,3]dioxole, and Furazan 2-oxide.
119. The method of claim 116, wherein n is selected from the group consisting ofO through 5.
120. The method of claim 116, wherein Zi is O.
121. The method of claim 116, wherein the Pin 1 -modulating compound of formula (I) is a compound of formula (II):
Figure imgf000149_0001
(II) wherein
R2, R3, and R5 are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, -
CN, NO2, F, CF3, OCF3, CO H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; Rt is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of -CH and N;
Ri is -(CH2)n-(X4)p-(CH2)m-CO2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; X is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1 ; wherein each CH2 group may be independently substituted with CrC6 alkyl, e.g. , CH3, CH2CH2SCH3, or OH.
-147-
122. The method of claim 116, wherein the Pinl-modulating compound of formula (I) is a compound of formula (III):
Figure imgf000150_0001
(in) wherein
R2, R3, and R4 are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R5 is H or lower alkyl, e.g. , CH3;
Xi, X2 and X3 are independently selected from the group consisting of -CH and N; Ri is -(CH2)n-(X )p-(CH2)m-Cθ2Re- wherein R6 is H or lower alkyl, e.g., t-butyl or CH2CH3; i is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1 ; wherein each CH2 group may be independently substituted with Cι-C6 alkyl, e.g., CH3, OH, or CH2CH2SCH3.
123. The method of claim 116, wherein said Pinl modulating compound is a Pinl inhibiting compound.
124. The method of claim 116, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
-148-
125. The method of claim 116, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
126. The method of claim 116, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
127. The method of claim 116, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
128. The method of claim 116, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
129. The method of claim 116, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
130. The method of claim 116, wherein said Pinl-modulating compound has a characteristic inhibition profile (CIP) and has a cytotoxicity effective to treat said Pinl- associated state.
131. The method of claim 130, wherein said Pin 1 -modulating compound has an IC50 value of less than about 40.
132. The method of claim 131, wherein said 1C50 value of between about 10 and about 40.
133. The method of claim 131, wherein said IC50 value of between about 1 and about 10.
134. The method of claim 131, wherein said IC50 value of less than about 1.
135. The method of claim 130, wherein said Pinl-modulating compound has a cytotoxicity of 3 μM or less as measured by the CBCA.
136. The method of claim 135, wherein said Pinl-modulating compound has a cytotoxicity of 1.5 μM or less as measured by the CBCA.
-149-
137. The method of claim 136, wherein said Pinl -modulating compound has a cytotoxicity of 1 μM or less as measured by the CBCA.
138. The method of any one of claims 116-137, wherein the hyperplastic inhibitory agent is tamoxifen.
139. The method of any one of claims 116-137, wherein the hyperplastic inhibitory agent is paclitaxel.
140. The method of any one of claims 116-137, wherein the hyperplastic inhibitory agent is docetaxel.
141. The method of any one of claims 116-137, wherein the hyperplastic inhibitory agent is interleukin-2.
142. The method of any one of claims 116-137, wherein the hyperplastic inhibitory agent is rituximab.
143. The method of any one of claims 116-137, wherein the hyperplastic inhibitory agent is tretinoin.
144. The method of any one of claims 116-137, wherein the hyperplastic inhibitory agent is methotrexate.
145. The method of any one of claims 116-137, wherein the hyperplastic inhibitory agent is a radiation therapy treatment.
-150-
146. A method for treating cancer in a subject comprising administering to a subject an effective amount of a combination of a Pinl-modulating compound of formula (I):
Figure imgf000153_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1;
Z and Zi are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ~, -S-, or - OCH2-, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof; Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino- 1 ,3 -dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide; and a hyperplastic inhibitory agent such that the cancer is treated.
147. The method of claim 146, wherein Z is S.
-151-
148. The method of claim 146, wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3-(methylene)-l-methyl-l,3-dihydro-indol-2-one, a benzo[l,3]dioxole, and Furazan 2-oxide.
149. The method of claim 146, wherein n is selected from the group consisting ofO through 5.
150. The method of claim 146, wherein Zi is O.
151. The method of claim 146, wherein the Pinl -modulating compound of formula (I) is a compound of formula (II):
Figure imgf000154_0001
(ID wherein
R2, R3, and R are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R4 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of-CH an N;
Ri is -(CH2)n-(X4)p-(CH2)m-CO2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with -Ce alkyl, e.g., CH3, CH2CH2SCH3, or OH.
-152-
152. The method of claim 146, wherein the Pin 1 -modulating compound of formula (I) is a compound of formula (III):
Figure imgf000155_0001
(III) wherein R2, R3, and R4 are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R5 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of-CH andN; Ri is -(CH2)n-(X4) -(CH2)m-CO2R6, wherein R6 is H or lower alkyl, e.g., t-butyl or CH2CH3; i is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Cι-C6 alkyl, e.g., CH3, OH, or CH2CH2SCH3.
153. The method of claim 146, wherein said Pinl modulating compound is a Pinl inhibiting compound.
154. The method of claim 146, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
155. The method of claim 146, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
-153-
156. The method of claim 146, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
157. The method of claim 146, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
158. The method of claim 146, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
159. The method of claim 146, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
160. A method for treating cyclin D 1 overexpression in a subject comprising administering to a subject an effective amount of a combination of a Pinl -modulating compound of formula (I):
Figure imgf000156_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is O or 1; Z and Zi are independently selected from the group consisting of
O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2O-, -S-, or - OCH -, wherein the aromatic groups may be substituted with one or more substituents
-154- selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
Ri is selected from the group consisting of H; -(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino-l,3-dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide; and a hyperplastic inhibitory agent such that the cyclin Dl overexpression is treated.
161. The method of claim 160, wherein Z is S.
162. The method of claim 160, wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3-(methylene)-l-meth l-l,3-dihydro-indol-2-one, a benzo[l,3]dioxole, and Furazan 2-oxide.
163. The method of claim 160, wherein n is selected from the group consisting of 0 through 5.
164. The method of claim 160, wherein Zi is O.
165. The method of claim 160, wherein the Pin 1 -modulating compound of formula (I) is a compound of formula (II):
Figure imgf000157_0001
(II)
-155- wherein
R2, R3, and Re are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R4 is H or lower alkyl, e.g., CH3; Xi, X2 and X3 are independently selected from the group consisting of-CH andN;
Ri is -(CH2)n-(X4)p-(CH2)m-CO2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1 ; wherein each CH2 group may be independently substituted with Ci-C6 alkyl, e.g., CH3, CH2CH2SCH3, or OH.
166. The method of claim 160, wherein the Pinl -modulating compound of formula (I) is a compound of formula (III):
Figure imgf000158_0001
(III) wherein
R2, R3, and R4 are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3,
CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4;
Rs is H or lower alkyl, e.g., CH3;
Xi, X and X3 are independently selected from the group consisting of-CH and N;
-156- Ri is -(CH2)n-(X4)p-(CH2)m-CO2Re, wherein Re is H or lower alkyl, e.g., t-butyl or CH2CH3; i is selected from the group consisting of -C(O)N-, -O-, -C(O>, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Ci-Ce alkyl, e.g. , CH3, OH, or CH2CH2SCH3.
167. The method of claim 160, wherein said Pinl modulating compound is a
Pinl inhibiting compound.
168. The method of claim 160, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
169. The method of claim 160, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
170. The method of claim 160, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
171. The method of claim 160, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
172. The method of claim 160, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
173. The method of claim 160, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
-157-
174. A Pinl -modulator comprising formula (I):
Figure imgf000160_0001
(I) wherein the dashed line indicates a single or a double bond; n is selected from the group consisting of 0 through 10; m is 0 or 1 ;
Z and Zi are independently selected from the group consisting of O or S;
AR is H or is selected from one or a combination of aromatic groups which may be directly linked or indirectly linked by alkylene, -S(O)2θ-, -S-, or - OCHr, wherein the aromatic groups may be substituted with one or more substituents selected from the group consisting of CH3, OEt, NO2, CO2H, CI, OH, F, Br, OCH3, CF3, OCF3, and -SO2CF3, OAc, -O-iBu, -S(O)2NH2, -CHO, C(O)CH3, -CN, CO2(CH2)qCH3, wherein q is an integer ranging from about 0 to 4, and any combination thereof;
Ri is selected from the group consisting of H; ~(X)pC(O)R2, wherein p is selected from the group consisting of 1 through 4, wherein X is CH2 or NH and which may be substituted with benzyl, wherein R2 is selected from the group consisting of OH, NR3 and phenyl, and wherein R3 is N-thiazol-2-yl- benzenesulfonamide; CH3; a carbocycle substituted or unsubstituted with OH or OEt; 3- imino-l,3-dihydro-indol-2-one; (2,6-Dichloro-benzylidene)-imine; and 4-methyl- benzenesulfonamide.
175. The Pinl -modulator of claim 174, wherein Z is S.
-158-
176. The Pinl -modulator of claim 174, wherein the aromatic group is selected from the group consisting of a pyridine, a phenyl, a furan, a thiophene, a pyrrole, a naphthalene, a pyrazole, a 3-(methylene)-l-methyl-l,3-dihydro-indol-2-one, a benzo[l,3]dioxole, and Furazan 2-oxide.
177. The Pinl -modulator of claim 174, wherein n is selected from the group consisting of 0 through 5.
178. The Pinl -modulator of claim 174, wherein Zi is O.
179. The Pin 1 -modulator of claim 174, wherein the Pin 1 -modulating compound of formula (I) is a compound of formula (II):
Figure imgf000161_0001
(II) wherein
R2, R3, and R6 are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4;
R4 is H or lower alkyl, e.g. , CH3; Xi, X2 and X3 are independently selected from the group consisting of-CH and N;
Ri is -(CH2)n-(X4)p-(CH2)m-CO2R5, wherein R5 is H or lower alkyl, e.g., t-butyl or CH2CH3; X4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with -Ce alkyl, e.g., CH3, CH2CH2SCH3, or OH.
-159-
180. The Pinl -modulator of claim 174, wherein the Pinl -modulating compound of formula (I) is a compound of formula (III):
Figure imgf000162_0001
(III) wherein R2, R3, and R4 are independently selected from the group consisting of H, OCH3, SO2CF3, -S(O2)NH2, OH, CI, C(O)CH3, - CN, NO2, F, CF3, OCF3, CO2H, CO2(CH2)qCH3, CH3, and Br, wherein q is an integer ranging from about 0 to 4; R5 is H or lower alkyl, e.g. , CH3; Xi, X2 and X3 are independently selected from the group consisting of-CH and N; Ri is -(CH2)n-(Xt)p-(CH2)m-Cθ2R6, wherein Re is H or lower alkyl, e.g., t-butyl or CH2CH3; 4 is selected from the group consisting of -C(O)N-, -O-, -C(O)-, -CHCH-; n is an integer number ranging from about 1 to 4; m is an integer number ranging from about 1 to 4; p is 0 or 1; wherein each CH2 group may be independently substituted with Ci-C6 alkyl, e.g., CH3, OH, or CH2CH2SCH3.
181. The Pinl -modulator of claim 174, wherein said Pinl modulating compound is a Pinl inhibiting compound.
182. The Pinl -modulator of claim 174, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
183. The Pinl -modulator of claim 174, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
• 160-
184. The Pinl -modulator of claim 174, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
185. The Pinl -modulator of claim 174, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
186. The Pinl -modulator of claim 174, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
187. The Pinl -modulator of claim 174, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
188. A pharmaceutical composition comprising a Pin 1 -modulating compound of claim 1, 38, 116, 146, 160, or 174, and a pharmaceutically acceptable carrier.
189. The pharmaceutical composition of claim 112, wherein said compound is selected from the group consisting of compounds listed in Table 1, and derivatives thereof.
190. The pharmaceutical composition of claim 188, wherein said compound is selected from the group consisting of compounds listed in Table 2, and derivatives thereof.
191. The pharmaceutical composition of claim 188, wherein said compound is selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
192. The pharmaceutical composition of claim 188, wherein said compound is selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
193. The pharmaceutical composition of claim 188, wherein said compound is selected from the group consisting of compounds listed in Table 5, and derivatives thereof.
-161-
194. The pharmaceutical composition of claim 188, wherein said compound is selected from the group consisting of compounds listed in Table 6, and derivatives thereof.
195. A compound selected from the group consisting of compounds listed in
Table 2, and derivatives thereof.
196. A compound selected from the group consisting of compounds listed in Table 3, and derivatives thereof.
197. A compound selected from the group consisting of compounds listed in Table 4, and derivatives thereof.
-162-
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Cited By (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004096199A2 (en) * 2003-05-02 2004-11-11 Scottish Biomedical Limited Regulation of guanine nucleotide exchange factor
WO2005040163A1 (en) * 2003-10-28 2005-05-06 Dr. Reddy's Laboratories Ltd Heterocyclic compounds that block the effects of advanced glycation end products (age)
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WO2006024699A1 (en) * 2004-08-30 2006-03-09 Karyon-Ctt Ltd Thioxothiazolidinone compounds for use as pharmaceuticals
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Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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US8068988B2 (en) 2003-09-08 2011-11-29 Ventana Medical Systems, Inc. Method for automated processing of digital images of tissue micro-arrays (TMA)
US20050136509A1 (en) * 2003-09-10 2005-06-23 Bioimagene, Inc. Method and system for quantitatively analyzing biological samples
US7566732B2 (en) * 2003-10-28 2009-07-28 Rigel Pharmaceuticals, Inc. Rhodanine compositions for use as antiviral agents
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US9598395B2 (en) 2012-03-23 2017-03-21 The Regents Of The University Of California Premature-termination-codons readthrough compounds
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001002377A1 (en) * 1999-07-01 2001-01-11 Geron Corporation Telomerase inhibitors and methods of their use
WO2001057006A1 (en) * 2000-02-03 2001-08-09 F. Hoffmann-La Roche Ag Thiazolidine carboxylic acid derivatives and their use in the treatment of cancer

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001002377A1 (en) * 1999-07-01 2001-01-11 Geron Corporation Telomerase inhibitors and methods of their use
WO2001057006A1 (en) * 2000-02-03 2001-08-09 F. Hoffmann-La Roche Ag Thiazolidine carboxylic acid derivatives and their use in the treatment of cancer

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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US8362252B2 (en) 2004-09-28 2013-01-29 Otsuka Pharmaceutical Co., Ltd. Carbostyril compound
US7777038B2 (en) 2004-09-28 2010-08-17 Otsuka Pharmaceutical Co., Ltd. Carbostyril compound
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US8119812B2 (en) 2009-04-20 2012-02-21 Sbi Biotech Co., Ltd. Thiazolidinone derivative
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US9303033B2 (en) 2009-12-04 2016-04-05 Senhwa Biosciences, Inc. Pyrazolopyrimidines and related heterocycles as CK2 inhibitors
JP2013512903A (en) * 2009-12-04 2013-04-18 サイリーン ファーマシューティカルズ インコーポレーティッド Pyrazolopyrimidines and related heterocyclic compounds as CK2 inhibitors
EP2509602A1 (en) * 2009-12-04 2012-10-17 Cylene Pharmaceuticals, Inc. Pyrazolopyrimidines and related heterocycles as ck2 inhibitors
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US8759536B2 (en) 2010-06-18 2014-06-24 Avixgen Inc. Rhodanine derivatives, method for preparing same, and pharmaceutical composition for the prevention or treatment of aids containing the rhodanine derivatives as active ingredients
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US12065416B2 (en) 2016-04-05 2024-08-20 Immunesensor Therapeutics, Inc. cGAS antagonist compounds
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