WO2004026772A3 - Ammonia-oxidizing bacteria and methods of using and detecting the same - Google Patents

Ammonia-oxidizing bacteria and methods of using and detecting the same Download PDF

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Publication number
WO2004026772A3
WO2004026772A3 PCT/US2003/028210 US0328210W WO2004026772A3 WO 2004026772 A3 WO2004026772 A3 WO 2004026772A3 US 0328210 W US0328210 W US 0328210W WO 2004026772 A3 WO2004026772 A3 WO 2004026772A3
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
ammonia
methods
present
oxidizing bacteria
Prior art date
Application number
PCT/US2003/028210
Other languages
French (fr)
Other versions
WO2004026772A2 (en
Inventor
Timothy A Hovanec
Original Assignee
Aquaria Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aquaria Inc filed Critical Aquaria Inc
Priority to CA002499482A priority Critical patent/CA2499482A1/en
Priority to AU2003272297A priority patent/AU2003272297B2/en
Priority to MXPA05002873A priority patent/MXPA05002873A/en
Priority to JP2004537749A priority patent/JP4470025B2/en
Priority to EP03754473A priority patent/EP1551773A2/en
Priority to BR0314125-0A priority patent/BR0314125A/en
Publication of WO2004026772A2 publication Critical patent/WO2004026772A2/en
Publication of WO2004026772A3 publication Critical patent/WO2004026772A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Biological Treatment Of Waste Water (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Described herein are novel ammonia-oxidizing bacteria as well as isolated nucleotide sequences representative of 16S rDNA of these ammonia-oxidizing bacteria. Particular bacteria of the present invention are tolerant of freshwater environments, saltwater environments or both. Furthermore, in various embodiments, various bacteria of the present invention are capable of surviving a freeze-drying process, and may remain viable thereafter. Compositions including various combinations of the bacteria are further described, as are polymerase chain reaction (PCR) primers and oligonucleotide probes that may be used to detect these bacteria based on their 16S rDNA. Methods for preventing or alleviating the accumulation of ammonia in aqueous environments, such as aquaria and wastewater are also provided, using the ammonia-oxidizing bacteria of the present invention. Methods for detecting the bacteria of the present invention are also provided. Such methods may be effected by any conventional methology, such as with a DNA chip.
PCT/US2003/028210 2002-09-19 2003-09-10 Ammonia-oxidizing bacteria and methods of using and detecting the same WO2004026772A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA002499482A CA2499482A1 (en) 2002-09-19 2003-09-10 Ammonia-oxidizing bacteria and methods of using and detecting the same
AU2003272297A AU2003272297B2 (en) 2002-09-19 2003-09-10 Ammonia-oxidizing bacteria and methods of using and detecting the same
MXPA05002873A MXPA05002873A (en) 2002-09-19 2003-09-10 Ammonia-oxidizing bacteria and methods of using and detecting the same.
JP2004537749A JP4470025B2 (en) 2002-09-19 2003-09-10 Ammonia-oxidizing bacteria and methods of use and detection thereof
EP03754473A EP1551773A2 (en) 2002-09-19 2003-09-10 Ammonia-oxidizing bacteria and methods of using and detecting the same
BR0314125-0A BR0314125A (en) 2002-09-19 2003-09-10 Ammonia oxidizing bacteria and processes for using and detecting them

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US38621702P 2002-09-19 2002-09-19
US38621902P 2002-09-19 2002-09-19
US38621802P 2002-09-19 2002-09-19
US60/386,218 2002-09-19
US60/386,217 2002-09-19
US60/386,219 2002-09-19

Publications (2)

Publication Number Publication Date
WO2004026772A2 WO2004026772A2 (en) 2004-04-01
WO2004026772A3 true WO2004026772A3 (en) 2004-05-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/028210 WO2004026772A2 (en) 2002-09-19 2003-09-10 Ammonia-oxidizing bacteria and methods of using and detecting the same

Country Status (7)

Country Link
EP (1) EP1551773A2 (en)
JP (2) JP4470025B2 (en)
AU (1) AU2003272297B2 (en)
BR (1) BR0314125A (en)
CA (1) CA2499482A1 (en)
MX (1) MXPA05002873A (en)
WO (1) WO2004026772A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1978100A1 (en) * 2007-04-03 2008-10-08 Stichting Geodelft Microbiologically induced carbonate precipitation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2252064A1 (en) * 1997-09-16 2000-05-20 Crc For Waste Management And Pollution Control Limited Aquatic nitrite oxidising microorganisms
WO2001090312A1 (en) * 2000-05-19 2001-11-29 Aquaria, Inc. Ammonia-oxidizing bacteria

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2252064A1 (en) * 1997-09-16 2000-05-20 Crc For Waste Management And Pollution Control Limited Aquatic nitrite oxidising microorganisms
WO2001090312A1 (en) * 2000-05-19 2001-11-29 Aquaria, Inc. Ammonia-oxidizing bacteria

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
AAKRA A ET AL: "RFLP OF RRNA GENES AND SEQUENCING OF THE 16S-23S RDNA INTERGENIC SPACER REGION OF AMMONIA-OXIDIZING BACTERIA: A PHYLOGENETIC APPROACH", INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 49, January 1999 (1999-01-01), pages 123 - 130, XP001029900, ISSN: 0020-7713 *
AAKRA AGOT ET AL: "Detailed phylogeny of ammonia-oxidizing bacteria determined by rDNA sequences and DNA homology values", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 51, no. 6, November 2001 (2001-11-01), pages 2021 - 2030, XP009026625, ISSN: 1466-5026 *
BURRELL P C ET AL: "Identification of ammonia-oxidizing bacteria responsible for nitrification in freshwater aquaria", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR, vol. 101, 2001, 101st General Meeting of the American Society for Microbiology;Orlando, FL, USA; May 20-24, 2001, 2001, pages 483, XP009026630, ISSN: 1060-2011 *
BURRELL PAUL C ET AL: "Identification of bacteria responsible for ammonia oxidation in freshwater aquaria", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 12, AF386746, December 2001 (2001-12-01), pages 5791 - 5800, XP002271945, ISSN: 0099-2240, Retrieved from the Internet <URL:EBI> *
DATABASE EMBL [online] XP002271947, retrieved from EBI Database accession no. AF386746 *
DATABASE EMBL [online] XP002271948, retrieved from EBI Database accession no. AF272420 *
HOVANEC T A ET AL: "Identification of ammonia- and nitrite-oxidizing bacteria responsible for nitrification in saltwater aquaria", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR, vol. 101, 2001, 101st General Meeting of the American Society for Microbiology;Orlando, FL, USA; May 20-24, 2001, 2001, pages 499 - 500, XP009026629, ISSN: 1060-2011 *
HOVANEC TIMOTHY A ET AL: "Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 62, no. 8, August 1996 (1996-08-01), pages 2888 - 2896, XP002267477, ISSN: 0099-2240 *
KOWALCHUK GEORGE A ET AL: "Ammonia-oxidizing bacteria: A model for molecular microbial ecology", ANNUAL REVIEW OF MICROBIOLOGY, 2001, Annual Reviews {a}, 4139 El Camino Way, Palo Alto, CA, 94303-0139, USA Series: Annual Review of Microbiology (ISSN 0066-4227), pages 485 - 529, XP009026626 *
PURKHOLD ULRIKE ET AL: "Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: Implications for molecular diversity surveys.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 12, AF272420, December 2000 (2000-12-01), pages 5368 - 5382, XP002271946, ISSN: 0099-2240 (ISSN print), Retrieved from the Internet <URL:EBI> *

Also Published As

Publication number Publication date
MXPA05002873A (en) 2005-06-22
AU2003272297A1 (en) 2004-04-08
WO2004026772A2 (en) 2004-04-01
AU2003272297B2 (en) 2006-09-14
BR0314125A (en) 2005-06-28
CA2499482A1 (en) 2004-04-01
EP1551773A2 (en) 2005-07-13
JP2006500027A (en) 2006-01-05
JP4470025B2 (en) 2010-06-02
JP2010057494A (en) 2010-03-18

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