WO2004025295A3 - Process and apparatus comprising episcopoc differential contrast (edic) microscopy plus epifluorescence microscopy (ef) for the detection or identification of biological materials on surfaces - Google Patents

Process and apparatus comprising episcopoc differential contrast (edic) microscopy plus epifluorescence microscopy (ef) for the detection or identification of biological materials on surfaces Download PDF

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Publication number
WO2004025295A3
WO2004025295A3 PCT/GB2003/004004 GB0304004W WO2004025295A3 WO 2004025295 A3 WO2004025295 A3 WO 2004025295A3 GB 0304004 W GB0304004 W GB 0304004W WO 2004025295 A3 WO2004025295 A3 WO 2004025295A3
Authority
WO
WIPO (PCT)
Prior art keywords
microscopy
identification
detection
edic
plus
Prior art date
Application number
PCT/GB2003/004004
Other languages
French (fr)
Other versions
WO2004025295A2 (en
Inventor
Eric Best
Charles William Keevil
Original Assignee
Univ Southampton
Eric Best
Charles William Keevil
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southampton, Eric Best, Charles William Keevil filed Critical Univ Southampton
Priority to EP03748265A priority Critical patent/EP1549947A2/en
Priority to JP2004535702A priority patent/JP2005539219A/en
Priority to AU2003267573A priority patent/AU2003267573A1/en
Publication of WO2004025295A2 publication Critical patent/WO2004025295A2/en
Publication of WO2004025295A3 publication Critical patent/WO2004025295A3/en
Priority to US11/081,493 priority patent/US20050208475A1/en

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/14—Condensers affording illumination for phase-contrast observation

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a microscopy system for rapid and sensitive detection and Identification of biological material on surfaces by implementing episcopic differential contrast (EDIC) microscopy plus epifluorescence (EF) microscopy wherein the microscope incorporates a DIC prism in the nosepiece and long distance objectives so that the materials can be visualised without requirement for a coverslip or oil or water immersion. The system is particularly useful for the detection and identification of infectious diseases.
PCT/GB2003/004004 2002-09-16 2003-09-16 Process and apparatus comprising episcopoc differential contrast (edic) microscopy plus epifluorescence microscopy (ef) for the detection or identification of biological materials on surfaces WO2004025295A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP03748265A EP1549947A2 (en) 2002-09-16 2003-09-16 Process and apparatus comprising episcopoc differential contrast (edic) microscopy plus epifluorescence microscopy (ef) for the detection or identification of biological material on surfaces
JP2004535702A JP2005539219A (en) 2002-09-16 2003-09-16 Novel method and apparatus
AU2003267573A AU2003267573A1 (en) 2002-09-16 2003-09-16 Process and apparatus comprising episcopoc differential contrast (edic) microscopy plus epifluorescence microscopy (ef) for the detection or identification of biological materials on surfaces
US11/081,493 US20050208475A1 (en) 2002-09-16 2005-03-16 Process and apparatus comprising episcopic differential contrast (EDIC) microscopy plus epifluorescence microscopy (EF) for the detection or identification of biological materials on surfaces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0221467A GB0221467D0 (en) 2002-09-16 2002-09-16 Novel process and apparatus
GB0221467.4 2002-09-16

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/081,493 Continuation-In-Part US20050208475A1 (en) 2002-09-16 2005-03-16 Process and apparatus comprising episcopic differential contrast (EDIC) microscopy plus epifluorescence microscopy (EF) for the detection or identification of biological materials on surfaces

Publications (2)

Publication Number Publication Date
WO2004025295A2 WO2004025295A2 (en) 2004-03-25
WO2004025295A3 true WO2004025295A3 (en) 2004-06-03

Family

ID=9944154

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/004004 WO2004025295A2 (en) 2002-09-16 2003-09-16 Process and apparatus comprising episcopoc differential contrast (edic) microscopy plus epifluorescence microscopy (ef) for the detection or identification of biological materials on surfaces

Country Status (5)

Country Link
EP (1) EP1549947A2 (en)
JP (1) JP2005539219A (en)
AU (1) AU2003267573A1 (en)
GB (1) GB0221467D0 (en)
WO (1) WO2004025295A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007075595A2 (en) * 2005-12-20 2007-07-05 Vertex Pharmacueticals Incorporated Biofilm assay
GB201014016D0 (en) * 2010-08-20 2010-10-06 Synoptics Ltd Imaging system and associated method for detection of protein contamination
US9588129B2 (en) 2013-03-15 2017-03-07 Amira Medical Technologies Inc. Methods for analyzing blood to detect diseases associated with abnormal protein aggregation
CN109283307B (en) * 2018-09-19 2021-06-11 中国地质科学院水文地质环境地质研究所 Evaluation method for natural degradation capability of pollutants in petrochemical engineering polluted site
EP4249889A1 (en) * 2022-03-23 2023-09-27 Nebulum Technologies Co., Ltd. Methods for preparing and analyzing biopsies and biological samples

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AZEVEDO N F ET AL: "Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms.", WATER SCIENCE AND TECHNOLOGY, vol. 47, no. 5, 28 March 2003 (2003-03-28), pages 155 - 160, XP001179846, ISSN: 0273-1223 *
ITURRIAGA RODOLFO ET AL: "Detection of respiratory enzyme activity in Giardia cysts and Cryptosporidium oocysts using redox dyes and immunofluoresce techniques", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 46, no. 1, 30 July 2001 (2001-07-30), pages 19 - 28, XP001180151, ISSN: 0167-7012 *
KEEVIL C W ET AL: "NORMARSKI DIC MICROSCOPY AND IMAGE ANALYSIS OF BIOFILMS SOCIETY FOR GENERAL MICROBIOLOGY UNIVERSITY OF BRISTOL UK JANUARY 8-9 1992", BINARY COMPUTING IN MICROBIOLOGY, vol. 4, no. 3, 1992, pages 93 - 95, XP009026856, ISSN: 1057-350X *
KEEVIL C W: "Rapid detection of biofilms and adherent pathogens using scanning confocal laser microscopy and episcopic differential interference contrast microscopy.", WATER SCIENCE AND TECHNOLOGY, vol. 47, no. 5, 28 March 2003 (2003-03-28), pages 105 - 116, XP001179845, ISSN: 0273-1223 *
ROGERS J ET AL: "IMMUNOGOLD AND FLUORESCEIN IMMUNOLABELLING OF LEGIONELLA-PNEUMOPHILA WITHIN AN AQUATIC BIOFILM VISUALIZED BY USING EPISCOPIC DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 58, no. 7, 1992, pages 2326 - 2330, XP009026855, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
AU2003267573A1 (en) 2004-04-30
EP1549947A2 (en) 2005-07-06
AU2003267573A8 (en) 2004-04-30
GB0221467D0 (en) 2002-10-23
WO2004025295A2 (en) 2004-03-25
JP2005539219A (en) 2005-12-22

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