WO2004017040A3 - Multi-dimensional chromatography system for proteomics - Google Patents

Multi-dimensional chromatography system for proteomics Download PDF

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Publication number
WO2004017040A3
WO2004017040A3 PCT/US2003/025367 US0325367W WO2004017040A3 WO 2004017040 A3 WO2004017040 A3 WO 2004017040A3 US 0325367 W US0325367 W US 0325367W WO 2004017040 A3 WO2004017040 A3 WO 2004017040A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteomics
multidimensional
chromatography system
abundant
dimensional chromatography
Prior art date
Application number
PCT/US2003/025367
Other languages
French (fr)
Other versions
WO2004017040A2 (en
Inventor
Thierry Baussant
Guenter Boehm
Manfred Heller
Martin Kussmann
Laure Menin
John Rogers
Keith Rose
Original Assignee
Geneprot Inc
Thierry Baussant
Guenter Boehm
Manfred Heller
Martin Kussmann
Laure Menin
John Rogers
Keith Rose
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0221469A external-priority patent/GB0221469D0/en
Application filed by Geneprot Inc, Thierry Baussant, Guenter Boehm, Manfred Heller, Martin Kussmann, Laure Menin, John Rogers, Keith Rose filed Critical Geneprot Inc
Priority to EP03788440A priority Critical patent/EP1535053A2/en
Priority to AU2003258208A priority patent/AU2003258208A1/en
Priority to CA002494998A priority patent/CA2494998A1/en
Publication of WO2004017040A2 publication Critical patent/WO2004017040A2/en
Publication of WO2004017040A3 publication Critical patent/WO2004017040A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/884—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample organic compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to multidimensional chromatography systems for proteomics and methods for identifying lower-abundance proteins in body fluids. Abundant proteins(s) is first removed by affinity chromatography utilizing affinity chromatographic column functionalized with ligand capable of selectively binding the abundant protein in the sample. Multidimensional chromatographic techniques are applied to the eluent with a reduced content of the abundant protein followed by mass spectrometric analysis.
PCT/US2003/025367 2002-08-16 2003-08-13 Multi-dimensional chromatography system for proteomics WO2004017040A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP03788440A EP1535053A2 (en) 2002-08-16 2003-08-13 Multi-dimensional chromatography system for proteomics
AU2003258208A AU2003258208A1 (en) 2002-08-16 2003-08-13 Multi-dimensional chromatography system for proteomics
CA002494998A CA2494998A1 (en) 2002-08-16 2003-08-13 Multi-dimensional chromatography system for proteomics

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40390902P 2002-08-16 2002-08-16
US60/403,909 2002-08-16
GB0221469.0 2002-09-16
GB0221469A GB0221469D0 (en) 2002-09-16 2002-09-16 Multi-dimensional chromatography system for proteomics

Publications (2)

Publication Number Publication Date
WO2004017040A2 WO2004017040A2 (en) 2004-02-26
WO2004017040A3 true WO2004017040A3 (en) 2004-10-21

Family

ID=31889686

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/025367 WO2004017040A2 (en) 2002-08-16 2003-08-13 Multi-dimensional chromatography system for proteomics

Country Status (4)

Country Link
EP (1) EP1535053A2 (en)
AU (1) AU2003258208A1 (en)
CA (1) CA2494998A1 (en)
WO (1) WO2004017040A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7528368B2 (en) 2006-03-23 2009-05-05 Protea Biosciences, Inc. Electrospray ionization process and add-on device with sample injection tip
US7641778B2 (en) 2006-03-23 2010-01-05 Protea Biosciences, Inc. Gel electroelution and sample separation devices and associated processes
US8074494B2 (en) 2007-12-10 2011-12-13 Proxeon Biosystems A/S Plugged flow 2D chromatography
CN103063753B (en) * 2012-08-31 2015-07-29 西安奥岚科技开发有限责任公司 For multidimensional liquid chromatographic separation system and the separation method of Protein Separation
DE102017100189A1 (en) * 2017-01-06 2018-07-12 Technische Universität Braunschweig Method for two-dimensional protein separation and protein separation device
US10908166B2 (en) * 2018-01-31 2021-02-02 Regeneron Pharmaceuticals, Inc. Dual-column LC-MS system and methods of use thereof
CN114088861A (en) * 2021-10-27 2022-02-25 上海市食品药品检验研究院 Method for detecting enterotoxin C in milk by using multi-dimensional liquid chromatography-mass spectrometry

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
"Multidimensional chromatography", 18 July 2002 (2002-07-18), XP002975235, Retrieved from the Internet <URL:http://www.chem.agilent.com/cag/prod/lc/Application-multi.pdf> *
ADKINS J.N. ET AL.: "Toward a human blood serum proteome", MOLECULAR AND CELLULAR PROTEOMICS, vol. 1, 13 November 2002 (2002-11-13), pages 947 - 955, XP002975238 *
ARNAUD P. ET AL.: "Purification of proteins by subtraction affinity chromatography", PROTIDES OF THE BIOLOGICAL FLUIDS, vol. 26, 1979, pages 649 - 652 *
FERGUSON P.L., SMITH R.D.: "Proteome analysis by mass spectrometry", ANNU. RE. BIOPHYS. STRUCT., vol. 32, 24 January 2003 (2003-01-24), pages 399 - 424, XP002975239 *
REGNIER F. ET AL.: "Multidimensional chromatography and the signature peptide approach to proteomics", LCGC, vol. 19, no. 2, February 2001 (2001-02-01), pages 200, 202, 204, 206, 208, 210, 212, 213, XP002975237 *
WOLTERS D.A. ET AL.: "An automated multidimensional protein identification technology for shotgun proteomics", ANAL. CHEM., vol. 73, 2001, pages 5683 - 5690, XP002975236 *
ZHANG Z. ET AL.: "Multiple separations facilitate identification of protein variants by mass spectrometry", PROTEOMICS, vol. 1, 2001, pages 1001 - 1009, XP009016604 *

Also Published As

Publication number Publication date
AU2003258208A8 (en) 2004-03-03
EP1535053A2 (en) 2005-06-01
AU2003258208A1 (en) 2004-03-03
WO2004017040A2 (en) 2004-02-26
CA2494998A1 (en) 2004-02-26

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