WO2004016651A3 - Spider silk polypeptide - Google Patents

Spider silk polypeptide Download PDF

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Publication number
WO2004016651A3
WO2004016651A3 PCT/GB2003/003578 GB0303578W WO2004016651A3 WO 2004016651 A3 WO2004016651 A3 WO 2004016651A3 GB 0303578 W GB0303578 W GB 0303578W WO 2004016651 A3 WO2004016651 A3 WO 2004016651A3
Authority
WO
WIPO (PCT)
Prior art keywords
spider silk
silk polypeptide
polypeptide
silk
sequencing
Prior art date
Application number
PCT/GB2003/003578
Other languages
French (fr)
Other versions
WO2004016651A2 (en
Inventor
Mason Simon Mcqueen
Natalia Pouchkina
Original Assignee
Univ York
Mason Simon Mcqueen
Natalia Pouchkina
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ York, Mason Simon Mcqueen, Natalia Pouchkina filed Critical Univ York
Priority to AU2003260719A priority Critical patent/AU2003260719A1/en
Publication of WO2004016651A2 publication Critical patent/WO2004016651A2/en
Publication of WO2004016651A3 publication Critical patent/WO2004016651A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

This invention describes the isolation and sequencing of a new silk polypeptide and includes vectors, transgenic plants and animals expressing DNA molecules encoding said polypeptide and methods for the production of said silk polypeptides.
PCT/GB2003/003578 2002-08-15 2003-08-15 Spider silk polypeptide WO2004016651A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003260719A AU2003260719A1 (en) 2002-08-15 2003-08-15 Spider silk polypeptide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0218977.7 2002-08-15
GB0218977A GB0218977D0 (en) 2002-08-15 2002-08-15 Polypeptide

Publications (2)

Publication Number Publication Date
WO2004016651A2 WO2004016651A2 (en) 2004-02-26
WO2004016651A3 true WO2004016651A3 (en) 2004-08-19

Family

ID=9942357

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/003578 WO2004016651A2 (en) 2002-08-15 2003-08-15 Spider silk polypeptide

Country Status (3)

Country Link
AU (1) AU2003260719A1 (en)
GB (1) GB0218977D0 (en)
WO (1) WO2004016651A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0405045D0 (en) 2004-03-05 2004-04-07 Spinox Ltd Composite materials
KR101348507B1 (en) * 2005-08-29 2014-01-06 엠실크 게엠베하 Modified spider silk proteins
CN101321777B (en) 2005-10-05 2015-10-21 联邦科学工业研究组织 Silk-protein
JP5192391B2 (en) 2005-12-30 2013-05-08 スパイバー・テクノロジーズ・アーベー Spider silk protein and spider silk protein production method
BRPI0701826B1 (en) 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária spider web proteins nephilengys cruentata, avicularia juruensis and parawixia bistriata isolated from Brazilian biodiversity
US8114631B2 (en) 2008-09-17 2012-02-14 The University Of Wyoming Nucleic acids encoding spider glue proteins and methods of use thereof
WO2011022771A1 (en) 2009-08-26 2011-03-03 Commonwealth Scientific Industrial Research Organisation Processes for producing silk dope
US8703425B2 (en) * 2011-09-12 2014-04-22 The Chinese University Of Hong Kong Biomarker for gastric cancer

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999047661A2 (en) * 1998-03-17 1999-09-23 Nexia Biotechnologies, Inc. Production of biofilaments in transgenic animals
WO2001090389A2 (en) * 2000-05-25 2001-11-29 E.I. Dupont De Nemours And Company Production of silk-like proteins in plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999047661A2 (en) * 1998-03-17 1999-09-23 Nexia Biotechnologies, Inc. Production of biofilaments in transgenic animals
WO2001090389A2 (en) * 2000-05-25 2001-11-29 E.I. Dupont De Nemours And Company Production of silk-like proteins in plants

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ARCIDIACONO S ET AL: "Purification and characterization of recombinant spider silk expressed in Escherichia coli", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 49, no. 1, January 1998 (1998-01-01), pages 31 - 38, XP002284484, ISSN: 0175-7598 *
BECKWITT R ET AL: "Sequence conservation in the C-terminal region of spider silk proteins (Spidroin) from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidae)", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 269, no. 9, 4 March 1994 (1994-03-04), pages 6661 - 6663, XP002243783, ISSN: 0021-9258 *
DATABASE EMBL 26 February 1998 (1998-02-26), "Nephila clavipes spidroin 1 mRNA, partial cds.", XP002284487, retrieved from EBI, HINXTON, UK Database accession no. U20329 *
DATABASE EMBL 9 April 2001 (2001-04-09), J. GATESY ET AL: "Dolomedes tenebrosus fibroin 2 mRNA, partial cds", XP002284485, retrieved from EBI, HINXTON, UK Database accession no. AF350270 *
DATABASE EMBL 9 April 2001 (2001-04-09), J. GATESY ET AL: "Lactrodectus geometricus major ampullate spidroin 1 (MaSp1) mRNA, partial cds", XP002284486, retrieved from EBI, HINXTON, UK Database accession no. AF350273 *
GATESY JOHN ET AL: "Extreme diversity, conservation, and convergence of spider silk fibroin sequences", SCIENCE (WASHINGTON D C), vol. 291, no. 5513, 30 March 2001 (2001-03-30), pages 2603 - 2605, XP001191057, ISSN: 0036-8075 *
GUERETTE P A ET AL: "SILK PROPERTIES DETERMINED BY GLAND-SPECIFIC EXPRESSION OF A SPIDER FIBROIN GENE FAMILY", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 272, no. 5258, 5 April 1996 (1996-04-05), pages 112 - 115, XP008018215, ISSN: 0036-8075 *
MING XU ET AL: "Structure of a protein superfiber: spider drag line", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 87, September 1990 (1990-09-01), pages 7120 - 7124, XP002120420, ISSN: 0027-8424 *
RIEKEL C ET AL: "Spider silk fibre extrusion: Combined wide- and small-angle X-ray microdiffraction experiments", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 29, no. 3, 22 October 2001 (2001-10-22), pages 203 - 210, XP002284483, ISSN: 0141-8130 *

Also Published As

Publication number Publication date
AU2003260719A1 (en) 2004-03-03
WO2004016651A2 (en) 2004-02-26
GB0218977D0 (en) 2002-09-25
AU2003260719A8 (en) 2004-03-03

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