WO2003106973A3 - METHODS AND APPARATUS FOR LOW RESISTANCE ELECTROPHORESIS OF PRE-MOLDING HYDRATABLE SEPARATION MEDIA - Google Patents

METHODS AND APPARATUS FOR LOW RESISTANCE ELECTROPHORESIS OF PRE-MOLDING HYDRATABLE SEPARATION MEDIA Download PDF

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Publication number
WO2003106973A3
WO2003106973A3 PCT/US2003/019335 US0319335W WO03106973A3 WO 2003106973 A3 WO2003106973 A3 WO 2003106973A3 US 0319335 W US0319335 W US 0319335W WO 03106973 A3 WO03106973 A3 WO 03106973A3
Authority
WO
WIPO (PCT)
Prior art keywords
molding
methods
separation media
media
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/019335
Other languages
French (fr)
Other versions
WO2003106973A2 (en
Inventor
Regina D Rooney
Bradley S Scott
Joseph W Amshey
Thomas R Jackson
Sheldon Engelhorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Priority to EP03760464A priority Critical patent/EP1525464A2/en
Priority to JP2004513742A priority patent/JP2005530151A/en
Priority to AU2003253661A priority patent/AU2003253661A1/en
Priority to CA002489725A priority patent/CA2489725A1/en
Publication of WO2003106973A2 publication Critical patent/WO2003106973A2/en
Publication of WO2003106973A3 publication Critical patent/WO2003106973A3/en
Anticipated expiration legal-status Critical
Priority to US12/485,379 priority patent/US8034223B2/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrostatic Separation (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne des procédés et un appareil qui facilitent l'électrophorèse de milieux de séparation hydratables de pré-moulage, des bandes à gradient de pH immobilisé. Le procédé exploite le pouvoir de gonflement des milieux de séparation hydratables de pré-moulage après réhydratation, afin de maintenir les milieux logés dans un élément enveloppant qui permet une communication électrique espacée avec les milieux de séparation enveloppés. Cette communication électrique permet l'établissement d'un gradient de tension dans le milieu de séparation enveloppé suffisant pour induire une séparation des matières à analyser qui s'y trouvent. La présente invention concerne également des cassettes, des noyaux tampons, des systèmes et des trousses d'électrophorèse permettant de mettre en oeuvre les procédés selon cette invention.The present invention relates to methods and apparatus that facilitate electrophoresis of hydratable separation media from pre-molding, immobilized pH gradient bands. The method exploits the swelling power of the hydratable separation media of pre-molding after rehydration, in order to maintain the media housed in an enveloping element which allows spaced electrical communication with the wrapped separation media. This electrical communication allows the establishment of a voltage gradient in the enveloped separation medium sufficient to induce separation of the materials to be analyzed therein. The present invention also relates to cassettes, buffer cores, electrophoresis systems and kits for carrying out the methods according to this invention.

PCT/US2003/019335 2001-05-10 2003-06-17 Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media Ceased WO2003106973A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP03760464A EP1525464A2 (en) 2002-06-18 2003-06-17 Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
JP2004513742A JP2005530151A (en) 2002-06-18 2003-06-17 Method and apparatus for low resistance electrophoresis of precast hydratable separation media
AU2003253661A AU2003253661A1 (en) 2002-06-18 2003-06-17 Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
CA002489725A CA2489725A1 (en) 2002-06-18 2003-06-17 Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
US12/485,379 US8034223B2 (en) 2001-05-10 2009-06-16 Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39025902P 2002-06-18 2002-06-18
US60/390,259 2002-06-18

Publications (2)

Publication Number Publication Date
WO2003106973A2 WO2003106973A2 (en) 2003-12-24
WO2003106973A3 true WO2003106973A3 (en) 2004-03-25

Family

ID=29736693

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/019335 Ceased WO2003106973A2 (en) 2001-05-10 2003-06-17 Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media

Country Status (6)

Country Link
EP (1) EP1525464A2 (en)
JP (1) JP2005530151A (en)
CN (1) CN1675538A (en)
AU (1) AU2003253661A1 (en)
CA (1) CA2489725A1 (en)
WO (1) WO2003106973A2 (en)

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* Cited by examiner, † Cited by third party
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US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
HRP20151294T1 (en) 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
JP5906519B2 (en) * 2011-09-13 2016-04-20 国立大学法人 熊本大学 Protein separation method by two-dimensional electrophoresis
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN108603866B (en) * 2016-09-23 2021-07-09 圣母大学 Single-step capillary isoelectric focusing and analyte migration
CN112354572B (en) * 2019-07-11 2022-02-11 北京理工大学 Multidimensional microfluidic electrophoresis chip, detection device and detection method
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
CN116134308B (en) 2020-05-19 2025-05-27 10X基因组学有限公司 Electrophoresis cartridge and instrument
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
EP4491742B1 (en) 2020-09-18 2026-03-04 10X Genomics, Inc. Sample handling apparatus and image registration methods
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6113766A (en) * 1997-06-09 2000-09-05 Hoefer Pharmacia Biotech, Inc. Device for rehydration and electrophoresis of gel strips and method of using the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6113766A (en) * 1997-06-09 2000-09-05 Hoefer Pharmacia Biotech, Inc. Device for rehydration and electrophoresis of gel strips and method of using the same

Also Published As

Publication number Publication date
EP1525464A2 (en) 2005-04-27
AU2003253661A2 (en) 2003-12-31
WO2003106973A2 (en) 2003-12-24
CA2489725A1 (en) 2003-12-24
JP2005530151A (en) 2005-10-06
AU2003253661A1 (en) 2003-12-31
CN1675538A (en) 2005-09-28

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