WO2003106651A3 - Spermatogonial cell line - Google Patents

Spermatogonial cell line Download PDF

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Publication number
WO2003106651A3
WO2003106651A3 PCT/US2003/019037 US0319037W WO03106651A3 WO 2003106651 A3 WO2003106651 A3 WO 2003106651A3 US 0319037 W US0319037 W US 0319037W WO 03106651 A3 WO03106651 A3 WO 03106651A3
Authority
WO
WIPO (PCT)
Prior art keywords
spermatogonial
immortalized
cell line
differentiation
cells
Prior art date
Application number
PCT/US2003/019037
Other languages
French (fr)
Other versions
WO2003106651A2 (en
Inventor
Martin Dym
Li-Xin Feng
Original Assignee
Univ Georgetown
Martin Dym
Li-Xin Feng
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Georgetown, Martin Dym, Li-Xin Feng filed Critical Univ Georgetown
Priority to AU2003253652A priority Critical patent/AU2003253652A1/en
Publication of WO2003106651A2 publication Critical patent/WO2003106651A2/en
Publication of WO2003106651A3 publication Critical patent/WO2003106651A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Abstract

The invention provides methods for in vitro generation of a telomerase-immortalized spermatogonial cell line. The invention further provides for the generation of spermatocytes and spermatids from immortalized spermatogonial cells by differentiation in the presence of stem cell factor. This differentiation can occur in the absence of supportive cells. Immortalized spermatogonial cell lines provide powerful tools for elucidating molecular mechanisms of spermatogenesis and for screening and developing compounds which affect spermatogenisis. Furthermore, through genomic modification and transplantation techniques, this male germ cell line can be used to generate transgenic animals and in germ cell gene therapy.
PCT/US2003/019037 2002-06-18 2003-06-18 Spermatogonial cell line WO2003106651A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003253652A AU2003253652A1 (en) 2002-06-18 2003-06-18 Spermatogonial cell line

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38927702P 2002-06-18 2002-06-18
US60/389,277 2002-06-18

Publications (2)

Publication Number Publication Date
WO2003106651A2 WO2003106651A2 (en) 2003-12-24
WO2003106651A3 true WO2003106651A3 (en) 2004-02-26

Family

ID=29736621

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/019037 WO2003106651A2 (en) 2002-06-18 2003-06-18 Spermatogonial cell line

Country Status (2)

Country Link
AU (1) AU2003253652A1 (en)
WO (1) WO2003106651A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100569168B1 (en) * 2003-08-08 2006-04-07 (주)아비코아생명공학연구소 Method for Culturing Avian Spermatogonial Stem Cells and Avian Spermatogonial Stem Cells Prepared thereby
KR100569163B1 (en) * 2003-08-11 2006-04-07 (주)아비코아생명공학연구소 Method for Producing Avian Chimera Using Spermatogonial Cells and Avian Chimera
JP2007532128A (en) * 2004-04-13 2007-11-15 カレッジ オブ メディスン ポーチョン シーエイチエー ユニバーシティ インダストリー−アカデミック コーポレーション ファウンデーション In vitro methods for germline stem cell isolation, proliferation and differentiation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6475789B1 (en) * 1996-10-01 2002-11-05 University Technology Corporation Human telomerase catalytic subunit: diagnostic and therapeutic methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6475789B1 (en) * 1996-10-01 2002-11-05 University Technology Corporation Human telomerase catalytic subunit: diagnostic and therapeutic methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FENG L.X. ET AL.: "Generation and in vitro differentiation of a spermatogonial cell line", SCIENCE, vol. 297, 19 July 2002 (2002-07-19), pages 392 - 395, XP002971660 *

Also Published As

Publication number Publication date
AU2003253652A1 (en) 2003-12-31
AU2003253652A8 (en) 2003-12-31
WO2003106651A2 (en) 2003-12-24

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