WO2003106634A3 - Vectors for expression of hml-2 polypeptides - Google Patents

Vectors for expression of hml-2 polypeptides Download PDF

Info

Publication number
WO2003106634A3
WO2003106634A3 PCT/US2003/018666 US0318666W WO03106634A3 WO 2003106634 A3 WO2003106634 A3 WO 2003106634A3 US 0318666 W US0318666 W US 0318666W WO 03106634 A3 WO03106634 A3 WO 03106634A3
Authority
WO
WIPO (PCT)
Prior art keywords
hml
promoter
nucleic acid
sequence encoding
polypeptide
Prior art date
Application number
PCT/US2003/018666
Other languages
French (fr)
Other versions
WO2003106634A2 (en
Inventor
Stephen F Hardy
John J Donnelly Iii
Megede Jan T Zur
Original Assignee
Chiron Corp
Stephen F Hardy
John J Donnelly Iii
Megede Jan T Zur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp, Stephen F Hardy, John J Donnelly Iii, Megede Jan T Zur filed Critical Chiron Corp
Priority to EP03741966A priority Critical patent/EP1532161B1/en
Priority to JP2004513447A priority patent/JP2005535308A/en
Priority to US10/587,032 priority patent/US8518694B2/en
Priority to CA002489292A priority patent/CA2489292A1/en
Priority to AU2003276679A priority patent/AU2003276679A1/en
Priority to AT03741966T priority patent/ATE545651T1/en
Publication of WO2003106634A2 publication Critical patent/WO2003106634A2/en
Publication of WO2003106634A3 publication Critical patent/WO2003106634A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

A nucleic acid vector comprising: (i) a promoter; (ii) a sequence encoding a HML-2 polypeptide operably linked to said promoter; and (iii) a selectable marker. Preferred vectors comprise: (I) a eukaryotic promoter; (ii) a sequence encoding a HML-2 polypeptide downstream of and operably linked to said promoter; (iii) a prokaryotic selectable marker; (iv) a prokaryotic origin of replication; and (v) a eukaryotic transcription terminator downstream of and operably linked to said sequence encoding a HML-2 polypeptide. Vectors of the invention are particularly useful for expression of HML-2 polypeptides either in vitro (e.g. for later purification). Or in vivo (e.g. for nucleic acid immunization). They are well suited to nucleic acid immunization against prostrate tumors. A preferred HML-2 is PCAV, which is located in chromosome 22 at 20.428 megabases (22q11.2).
PCT/US2003/018666 2002-06-13 2003-06-13 Vectors for expression of hml-2 polypeptides WO2003106634A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP03741966A EP1532161B1 (en) 2002-06-13 2003-06-13 Vectors for expression of hml-2 polypeptides
JP2004513447A JP2005535308A (en) 2002-06-13 2003-06-13 HML-2 polypeptide expression vector
US10/587,032 US8518694B2 (en) 2002-06-13 2003-06-13 Nucleic acid vector comprising a promoter and a sequence encoding a polypeptide from the endogenous retrovirus PCAV
CA002489292A CA2489292A1 (en) 2002-06-13 2003-06-13 Vectors for expression of hml-2 polypeptides
AU2003276679A AU2003276679A1 (en) 2002-06-13 2003-06-13 Vectors for expression of hml-2 polypeptides
AT03741966T ATE545651T1 (en) 2002-06-13 2003-06-13 VECTORS FOR EXPRESSING HML-2 POLYPEPTIDES

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38883102P 2002-06-13 2002-06-13
US33883102P 2002-06-13 2002-06-13
US60/388,831 2002-06-13
US47218903P 2003-05-20 2003-05-20
US60/472,189 2003-05-20

Publications (2)

Publication Number Publication Date
WO2003106634A2 WO2003106634A2 (en) 2003-12-24
WO2003106634A3 true WO2003106634A3 (en) 2004-12-23

Family

ID=33556285

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/018666 WO2003106634A2 (en) 2002-06-13 2003-06-13 Vectors for expression of hml-2 polypeptides

Country Status (1)

Country Link
WO (1) WO2003106634A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT502292B1 (en) * 2005-05-11 2010-04-15 Avir Green Hills Biotechnology MELANOMA DIAGNOSIS
WO2020049169A1 (en) * 2018-09-06 2020-03-12 Centre Léon-Bérard Herv-k-derived antigens as shared tumor antigens for anti-cancer vaccine

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2468903A1 (en) 2001-12-07 2004-05-06 Chiron Corporation Endogenous retrovirus up-regulated in prostate cancer
EP1521594B1 (en) 2001-12-07 2013-10-02 Novartis Vaccines and Diagnostics, Inc. Endogenous retrovirus polypeptides linked to oncogenic transformation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BOESE A ET AL: "Human endogenous retrovirus protein cORF supports cell transformation and associates with the promyelocytic leukemia zinc finger protein", ONCOGENE, vol. 19, no. 38, 7 September 2000 (2000-09-07), pages 4328 - 4336, XP002903506 *

Also Published As

Publication number Publication date
WO2003106634A2 (en) 2003-12-24

Similar Documents

Publication Publication Date Title
TW200732472A (en) Method for the recombinant expression of a polypeptide
RO117190B1 (en) Nucleic acid construct and method for identifying the expression of heterologous gene in a plant
ATE510920T1 (en) DNA SEQUENCES FOR REGULATION OF TRANSCRIPTION
WO2002059336A3 (en) Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector
EP1712625A4 (en) An arabidopis thaliana transcription factoras well as the encoding gene and the use thereof
WO2001044276A3 (en) Moss genes from physcomitrella patens encoding proteins involved in the synthesis of tocopherols carotenoids and aromatic amino acids
JPH07502162A (en) Cysteine-reduced IL-6 mutein
DK0811071T3 (en) Amplification sequences derived from the desmin gene, vectors containing these sequences and their use for the production of proteins
EP0953641A3 (en) Polynucleotide molecules encoding neospora proteins
IL133832A0 (en) Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
WO2003106634A3 (en) Vectors for expression of hml-2 polypeptides
WO2007058267A8 (en) Novel protein and gene encoding the protein
US5151509A (en) Gene encoding serine protease inhibitor
AR032557A1 (en) REGULATING SEQUENCES IN PLANTS
AU2003276679A1 (en) Vectors for expression of hml-2 polypeptides
ATE441708T1 (en) GENES CODING PROTEINS INVOLVED IN CYTOKININ SYNTHESIS
WO2001098453A3 (en) Modified construct downstream of the initiation codon for recombinant protein overexpression
HUT52552A (en) Process for production of negf
WO2005058944A3 (en) Immunogenic peptides fragments of xage-1
DE602004029158D1 (en) Archäen-expressionssystem
AU2001251410A1 (en) 12303, a novel human twik molecule and uses thereof
EP1221485A3 (en) Polynucleotide molecules encoding neospora proteins
GB2244272A (en) NF-Y initiation factors
WO2004113361A3 (en) Novel prokineticin receptor isoforms and methods of use
FR2832422B1 (en) NUCLEOTIDE SEQUENCE ENCODING A MODIFIED PROTEIN OF INTEREST, EXPRESSION VECTOR AND PROCESS FOR OBTAINING SAME

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2489292

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2004513447

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2003741966

Country of ref document: EP

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWP Wipo information: published in national office

Ref document number: 2003741966

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10587032

Country of ref document: US