WO2003105660A2

WO2003105660A2 - Method for determining pathosis of biological objects

Info

Publication number: WO2003105660A2
Application number: PCT/RU2003/000379
Authority: WO
Inventors: Hiena Zalmanovna Brainina; Alla Vladimirovna Ivanova; Elena Nikolaevna Sharafutdinova; Marya Yurievna Rubtsova; Alisa Nikolaevna Kozitsina; Boris Mikhaylovich Sergeev
Original assignee: Uralsky Gosudarstvenniy Ekonomichesky Universitet; Obschestvo S Ogranichennoi Otvetstvennostju Nauchno-Proizvodstvennoe Vnedrencheskoe Predpriyatie "Iva"
Priority date: 2002-06-18
Filing date: 2003-06-17
Publication date: 2003-12-24
Also published as: RU2249217C2; WO2003105660A3

Abstract

The invention relates to electrochemical analysis methods, in particular to disease diagnosis which is used for analysing a biological object. The aim of said invention is to simplify an analysis and reduce the costs thereof. In order to produce an immunosensor as a transducer, a graphite thick-film electrode characterised by a high absorption capacity, a low cost and ease of production is used. An immunocomplex (antigen-antibody) is formed on said electrode and afterwards incubated in a nonimmune metal-labelled protein solution. A metal oxidation reaction is used as a signal, the concentration of said metal being proportional to the concentration of antibodies in the immunocomplex. The anode voltamperometry with liner potential sweep is used for measuring an analytic signal.

Description

The method of dividing hospitals

biological objects.

The invention is available in the area of electrical equipment.

analysis, and specifically for the diagnosis of clinical conditions

analysis of the biological object.

The currently used diagnostic methods require

of convenient and convenient facilities, τ. κ. They are based on

spectrometric registration of the analytical signal.

The methods used for this in this way as an antibody are simple.

human immunoglobulins are reserved for use with the enzyme

pyrexidase χρena. The process of conjugation is convenient,

consumer goods, business, business, business, urban

the macromolecule with a specific function is not stable. Β case

electric process of sharing the necessary entertainment

ADDITIONAL MEDIA SYSTEMS, WHICH ARE ALSO

unstable reagents.

The methods of dividing the hospitals in the room were known

use human serum albumin and bismuth ions in 2 quality models for immunoelektrochemical analysis.

A multiplexer used for conjugation of antibodies and

Ion of bismuth - diethyl diamine pentaacetate (ДΤΑ). SERVICE -

elec- trodes manufactured by the process of printing Ηa

Electric power incurred by inhumane injuries

serial albumin (PC), then incubate 30 minutes in

blocking solution (thick buffer ρΗ = 7.2, tween-20) and

standard product containing 10 mg / ml ChSΑ-DΤΑ-Βismut. Signal

They register with the method of invariable potential. Bert

a free reagent containing 1.0% of saline acid and 20

mg / ml Η§, drops on working efficiency. Incidentally, together

a dedicated connection after connection will result in an irreversible connection with

bismuth The potential of the program registers simultaneously with the process

Chemical Oxidation (I. λνаη§ аηά Β. Τϊаη "Τск-Ρϋт ΕΙΕΙτггЪΙΙΙΙΙΙΙΙΙΙΙΙΙΙ

Тиttiηοδеηδοг άазάά οη 8πϊρρт§ Ροϊеηйοтеιгϊс ϋеϊесгюη ο-Г а Μеϊаϊ Ιοη

Ι-аъеΙ. "ΑηаΙ.Сет. 1998, 70, 1682-1685.).

The process is an application in the process.

analysis of mercury compounds, which are toxic, and also

high labor intensity and complexity of the process.

The methods of dividing the medical devices are known, as well as

diagnoses ϊaηϊa-virus in the plasma of the κr. Analysis is based on 3 Formation of the antibody complex of the antigen antibody, with the following

cooperation in the production of antibodies with human antibodies,

labeled with pyrexidase. Β Reactive mixture adds Η ₂ 0 ₂ and ΚΙ.

The next defect of the trans-oxidase tag is in operation

amperometry. The enzyme serves as a catalyst for the reduction of iodide in

Iodine in the possession of the compound. They register the recovery of iodine (

Ι ιеЪ ΚгϊδЬаη, Αηάgeu b. ΟЫηά Шз, Ρϊateη Αϊаηазον, ΕЬι δат УϊΙкϊηδ,

Lt Μοηюua, and ηά Ρgeάegϊsk Τ. "Οзϊег "ϊаδϊ Αтгеготететгϊс Ιтгттоаδδау south

Ηаηϊаνϊшδ Ιηιесйοη ". Εϊесϊгοаηаϊуδϊδ 1996, 8, -Chz 12, 1131-1134).

The use of such a method is an application in the analysis.

a profitable unstable enzyme, and also necessary

the use of additional reagents (Η ₂ 0 ₂ and S). Detection

Markets are indirectly related to the oxidation of iodide, and only

then register the restoration of iodine.

The closest technical solution selected in the quality

In addition, they serve as a means of dividing the hospitals

biological objects, including the formation of an immune complex

at the turn of the elec- trode, the connection of the tag to the immune complex and

the next tag detection. When using it,

gold in the quality of an immunoelectrical tag with goose

immunoglobulins on human antibodies. (Μ. Β. Οοηζаϊеδ 4 Οagδϊa, Α. Сοδϊа Οагδϊа "Αάδοгρиνе δ1χϊρρт§ νοϊϊаттетгϊс Ьеааνϊοиг οι ^*

сοϊϊοϊάаϊ §ο1ά аηά ϊттшιο§ο1ά οη сагъοη ρаδϊе еϊесггоάе.

ΒϊοеΙесΙгоссетϊδггу аηά Βϊοеηег§е1; ϊсδ 38, (1995), 389-395.)

The following is an accessory method:

this method of antibody - goose immunoglobulins

human antibodies are other reagents, also this

The method is indispensable to either a single diagnostic significant, real

system for the separation of proteins or macromolecules.

The invention is directed to the disposal of the method.

analysis, decrease in its cost, increase in accessibility

Diagnostic results, expansion of the specimen is shared

OBJECT.

This is achieved through the use of electric power.

Thermal film-containing electronic circuit, immunocomplex on

Includes antigen, antibody and label, as a matter of fact, in the label

they use a whitewash of the white of non-immune sources and metals,

In addition to this, the patient is judged by the oxidation reaction.

metals.

Indicated distinctive features are significant. Use

TELEPLOMATIC ELECTRIC OWNERSHIP

HIGH SOCIAL FUNCTIONS ENSURES STABLE 5 immunization with subsequent incubation in

The production of a protein of non-immune origin, labeled with a metal.

The use of the protein of the nonimmune origin of the conjugate

As a metal, in the sense of a metal, it significantly cheaper and cheaper

Diagnostic process and, taking into account the assessment of the oxidation reaction

metal, increases the accuracy and accessibility of the division.

In FIG. 1, a general view of the general view of the film is shown.

bulk electricity, where 1 - a supply from a fiberglass, 2 -

Warehouse from the warehouse, 3rd layer of cement or cement, 4 -

Operating electric power. SUMMARY OF THE INVENTION

It is characterized by the following examples.

Fig. 2 shows anode voltammeter oxidation.

gold, registered, where 1 voltamper, received from

using an electric product that contains specific antibodies κ

tick-borne encephalitis virus, 2-voltamper program received from

using an electric product that does not contain specific antibodies κ

tick-borne encephalitis virus.

Fig. 3 shows anode voltammeter oxidation.

Serbia, where 1 is a voltampergamma received using

an elec- trode containing specific antibodies to tick-borne virus

encephalitis, 2-voltampergamma received using 6 electrodes that do not contain specific antibodies to virus

tick-borne encephalitis.

Figure 4 shows the anode voltammeters of oxidation.

Serbia, where 1 is a voltampergamma received using

an elec- trode containing specific antibodies to tick-borne virus

encephalitis, 2-voltampergamma received with the use of

an elec- trode that does not contain specific antibodies to virus

tick-borne encephalitis.

Figure 5 shows the anode voltamperms,

registered, where 1-voltamperam received from

using an elec- trode containing an antibody to alpha

FTPs, 2-voltamperm received with the use of

an elec- trode that does not contain antibodies to alpha-fetuses.

The method is illustrated by the following examples.

For example, ϊ.

Аче For convenience, you can choose a different thick film film

Electricity with a flat edge. On request from fiberglass 1

Deals from the Warehouse 2. After the stock is removed,

120 ° C emits an inoperative discharge of cement 3. Ce

Working Electricity 4 Apply Antigen (Virus)

tick-borne encephalitis). Received electricity with immobilized 7 incubate in the course of a patient at the end of 30 minutes

at a temperature of 37 ° C, then wash the electrical elements with a buffer

a condition containing normal normal horse serum (UHF) and

Tween-20. Then they incubate with the product of ρ, labeled

gallant gold. After that, the elec-

containing UCL and tween-20 and locate in a single cell,

where is an electrically-electrically-carbonized, electrically-comparated Α§-

Α§C1 elektrood, phon-0,1Μ hydrochloric acid Signal Registers

method anodic voltammetry at a potential of 1.0 Β (oxidation

gold) is a relatively rich, healthy, ..

Measurement of potential with the help of potential. For a good experience

use a live virus that does not contain a virus

tick-borne encephalitis. Scanning speed of 100 mΒ / s (Fig. 2). Β

The patient's body is found to be 0.002 mg / ml of specific antibodies.

on the fly of tick-borne encephalitis.

For example, 2.

To operate the electric appliance 4 apply the antigen product

(tick-borne encephalitis virus). Received power from

with an immobilized antigen they are incubated in the serum

The patient for 30 minutes at Τ = 37 ° C, then wash the electrodes

a buffer that contains a normal ад horse ’ 8 and Tween-20. Performs incubation with the product of the ρ, labeled

silver. After that, the elec-

containing UCL and tween-20 and locate in a single cell,

where is an electrically-electrically-carbonized, electrically-comparated Α§-

Α§C1 elec- tropod, phon-0.1 Μ cold calcium. Signal Registers

at the anode voltammetry at a potential of 0.05 Β

(oxidation of the serum) is a relatively rich, rich, cereal

food. Measurement of potential with the help of potential. For

A GOOD EXPERIENCE USES AN EXCEPT OF AN INFORMATION THAT DOES NOT CONTAIN AN ANIMAL

to the tick-borne encephalitis virus. Scanning speed 100 mΒ / s

(φig. 3). With the patient organism, 0.002 mg / ml is specific

Antibody with a tick-borne encephalitis virus.

NOTE

To operate the electric appliance 4 apply the antigen product

(tick-borne encephalitis virus). Received power from

with an immobilized antigen they are incubated in the serum

The patient for 30 minutes at Τ = 37 ° C, then wash the electrodes

a buffer that contains a normal ад horse ’

and Tween-20. Then they incubate with the product of ρ, labeled

silver. After that, the elec-

containing UCL and tween-20 and locate in a single cell, 9 where is an electrically-electrically-carbohydrate, electrical-comparing- Α§-

Α§C1 elec- tropod, phon-0.1 Μ cold calcium. Signal Registers

at the anode voltammetry at a potential of 0.05 Β

(oxidation of the serum) is a relatively rich, rich, cereal

food. Measurement of potential with the help of potential. For

to the tick-borne encephalitis virus. Scanning speed 100 mΒ / s

(Fig. 4). The patient's body needs 0.00002 mg / ml

specific antibodies with virus tick-borne encephalitis.

Example 4.

To operate the electric appliance 4 apply the antigen product

(alpha-fetosephrine). Received electricity with immobilized

Antigens Incubate in a Highly Specific Antibody Fraction

(immunoglobulins Ο from the hyperaimmune test) for 30

a minute and Τ = 37 ° C, then wash off the electrical elements with a buffer solution,

containing a normal horny serum and tween-20. Then

Incubate with a waste of Α, labeled silver. After what

The elec- tric is washing away from the back with a direct-linking power line and twin-20 and

Places in a electrical cell, where an auxiliary electrical

glass-carbon, elec- trode of comparison - Α§-Α§C1 electric, phon-0.1 ο

halya kaliya. Signal is registered by the anode method 10 voltammetry and potential 0.05 Β (oxidation of silver)

Relatively rich and clean all-electric food. Measurement

With the help of the potential. For a good experience use

buffered discharge ρρ and ρΗ = 7.2. The concentration of antibodies is 0.005 mg / ml.

Scanning speed of 100 mΒ / s (Fig. 5).

The use of the proposed method makes it possible to use and

reduce the cost of elec- tric analysis by counting

USE ON OTHER PRODUCTS

TELEPAPULAR ELECTRIC FOOD, MORE ACCESSIBLE FOR RADIATION AND

a cheap protein squirrel is not an immune device and metal,

By introducing in the proposed method of oxidation of the metal

anodic voltamperometry.

Claims

eleven

Description of the invention.

The method of dividing the biological processes of biological

products with protein, labeled with metal, including

The development of the immune complex at the turn of the elec- trode, label and

The following detachment of a tag for an assessment of a public housing,

characterized by the fact that in the quality of electricity is used

Thermal film-containing electronic circuit, immunocomplex on

Includes antigen, antibody and label, as a matter of fact, in the label

they use a whitewash of the white of non-immune sources and metals,

In addition to this, the patient is judged by the oxidation reaction.

metals.