WO2003104813A3 - Modification of polypeptides for characterisation purposes - Google Patents

Modification of polypeptides for characterisation purposes Download PDF

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Publication number
WO2003104813A3
WO2003104813A3 PCT/GB2003/002451 GB0302451W WO03104813A3 WO 2003104813 A3 WO2003104813 A3 WO 2003104813A3 GB 0302451 W GB0302451 W GB 0302451W WO 03104813 A3 WO03104813 A3 WO 03104813A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
polypeptides
peptides
aqueous medium
soluble
Prior art date
Application number
PCT/GB2003/002451
Other languages
French (fr)
Other versions
WO2003104813A2 (en
Inventor
Andrew Hugin Thompson
Christian Hamon
Karsten Kuhn
Ute Bauer
Thomas Meumann
Original Assignee
Xzillion Gmbh & Co Kg
Proteome Sciences Plc
Andrew Hugin Thompson
Christian Hamon
Karsten Kuhn
Ute Bauer
Thomas Meumann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/GB2002/002778 external-priority patent/WO2002099436A2/en
Priority claimed from PCT/GB2002/002601 external-priority patent/WO2002099124A2/en
Application filed by Xzillion Gmbh & Co Kg, Proteome Sciences Plc, Andrew Hugin Thompson, Christian Hamon, Karsten Kuhn, Ute Bauer, Thomas Meumann filed Critical Xzillion Gmbh & Co Kg
Priority to AU2003274157A priority Critical patent/AU2003274157A1/en
Publication of WO2003104813A2 publication Critical patent/WO2003104813A2/en
Publication of WO2003104813A3 publication Critical patent/WO2003104813A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Provided is a method of solubilising a polypeptide, which polypeptide is insoluble or sparingly soluble in an aqueous medium, which method comprises either: (a) contacting the polypeptide with a sequence specific cleavage agent in a non-aqueous medium, to produce a product which is soluble in an aqueous medium; or (b) contacting the polypeptide with a dicarboxylic anhydride to modify one or more amino groups in the polypeptide, to produce a product which is soluble in an aqueous medium. Also provided is a relational database comprising: (a) a table of parent polypeptide sequences; and (b) a table of peptides, which peptides are expected products of putative reactions of polypeptides from the table of polypeptides; wherein each of the parent polypeptide sequences is relatable by the database to one or more peptides from the table of peptides.
PCT/GB2003/002451 2002-06-07 2003-06-06 Characterising polypeptides WO2003104813A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003274157A AU2003274157A1 (en) 2002-06-07 2003-06-06 Modification of polypeptides for characterisation purposes

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GBPCT/GB02/02601 2002-06-07
PCT/GB2002/002778 WO2002099436A2 (en) 2001-06-07 2002-06-07 Characterising polypeptides
GBPCT/GB02/02778 2002-06-07
PCT/GB2002/002601 WO2002099124A2 (en) 2001-06-07 2002-06-07 Characterising polypeptides
EP02257095 2002-10-14
EP02257095.6 2002-10-14

Publications (2)

Publication Number Publication Date
WO2003104813A2 WO2003104813A2 (en) 2003-12-18
WO2003104813A3 true WO2003104813A3 (en) 2004-06-03

Family

ID=56290437

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/002451 WO2003104813A2 (en) 2002-06-07 2003-06-06 Characterising polypeptides

Country Status (2)

Country Link
AU (1) AU2003274157A1 (en)
WO (1) WO2003104813A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD237749A3 (en) * 1983-12-23 1986-07-30 7010 Leipzig,Karl-Marx-Platz,Dd PROCESS FOR THE PRODUCTION OF SOLUBLE GLIADINES
WO1988001511A1 (en) * 1986-09-04 1988-03-10 Cetus Corporation Succinylated interleukin-2 for pharmaceutical compositions
EP0578472A2 (en) * 1992-07-07 1994-01-12 Sankyo Company Limited A process for recovering peptides expressed as fusion proteins
WO2002031509A2 (en) * 2000-10-11 2002-04-18 Compugen Ltd. Method for determining mass altering moiety in peptides
WO2002042427A2 (en) * 2000-10-25 2002-05-30 Surromed, Inc. Mass tags for quantitative analysis

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD237749A3 (en) * 1983-12-23 1986-07-30 7010 Leipzig,Karl-Marx-Platz,Dd PROCESS FOR THE PRODUCTION OF SOLUBLE GLIADINES
WO1988001511A1 (en) * 1986-09-04 1988-03-10 Cetus Corporation Succinylated interleukin-2 for pharmaceutical compositions
EP0578472A2 (en) * 1992-07-07 1994-01-12 Sankyo Company Limited A process for recovering peptides expressed as fusion proteins
WO2002031509A2 (en) * 2000-10-11 2002-04-18 Compugen Ltd. Method for determining mass altering moiety in peptides
WO2002042427A2 (en) * 2000-10-25 2002-05-30 Surromed, Inc. Mass tags for quantitative analysis

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DEYL Z ET AL: "Advanced separation methods for collagen parent alpha-chains, their polymers and fragments", JOURNAL OF CHROMATOGRAPHY. BIOMEDICAL APPLICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 739, no. 1, February 2000 (2000-02-01), pages 3 - 31, XP004190283, ISSN: 0378-4347 *
EL-ADAWY TAREK A: "Functional properties and nutritional quality of acetylated and succinylated mung bean protein isolate", FOOD CHEMISTRY, vol. 70, no. 1, July 2000 (2000-07-01), pages 83 - 91, XP002259200, ISSN: 0308-8146 *
ORTWERTH B J ET AL: "Chemical modification of alpha crystallin", EXPERIMENTAL EYE RESEARCH, ACADEMIC PRESS LTD., LONDON, GB, vol. 56, no. 1, 1993, pages 107 - 114, XP001037649, ISSN: 0014-4835 *
PALACIAN E ET AL: "DICARBOXYLIC ACID ANHYDRIDES AS DISSOCIATING AGENTS OF PROTEIN- CONTAINING STRUCTURES", MOLECULAR AND CELLULAR BIOCHEMISTRY, NORWELL, MA, US, vol. 97, no. 2, 1990, pages 101 - 111, XP000610050, ISSN: 0300-8177 *

Also Published As

Publication number Publication date
AU2003274157A8 (en) 2003-12-22
WO2003104813A2 (en) 2003-12-18
AU2003274157A1 (en) 2003-12-22

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