WO2003098996A1

WO2003098996A1 - Tree plantation method

Info

Publication number: WO2003098996A1
Application number: PCT/JP2003/005572
Authority: WO
Inventors: Takayuki Asada
Original assignee: Oji Paper Co., Ltd.
Priority date: 2002-05-09
Filing date: 2003-04-30
Publication date: 2003-12-04
Also published as: JP4244564B2; NZ534382A; CN1638620A; JP2003325063A; CN1314313C; AU2003235825B2; AU2003235825A1; BR0304714A

Abstract

It is intended to provide a tree transplantation method which burdens a less environmental load or risk compared with gene recombination techniques and by which plantation in an area under severe environmental stress can be performed by fully utilizing the inherent ability of plants per se. By supplying water containing CO2 or O2 dissolved therein to a planted seedling, plantation can be carried out in an area under severe environmental stress. This seedling is sowed and grown after allowing a seedling for plantation to absorb a substance inhibiting the function of cytochrome P450 from the above-ground part, the under-ground part or both, or allowing a seed for plantation to absorb the substance.

Description

Description Tree planting method Technology field

The present invention relates to a tree planting method, in particular, by planting tree planting seedlings having improved resistance to environmental stress from their original properties, or by supplying various kinds of gas-dissolved water to the planted tree seedlings. Or by providing various gas-dissolved water to planted seedlings with higher resistance to environmental stress than the original property, to further enhance resistance to environmental stress. It relates to a tree planting method that aims to enable tree planting in areas with severe environmental stress by combining the two. Background technology

The development of industrial technology that contributes to global environmental issues plays an important role and expectations. For this reason, a wide variety of efforts are being made in the industrial world to pursue practical applications, not only basic research, but also advanced applied technology development. For example, we will create plants that are resistant to various environmental stresses (salt tolerance, drought tolerance, cold tolerance, etc.), use these plants to green desert, salt-affected areas, cold areas, etc., and continue to increase on the earth Genetically imparts environmental stress resistance to plants by introducing genes responsible for the environmental stress resistance mechanism into plants (genetical recombination technology) to help reduce carbon dioxide R & D is underway to make it happen. Such a thing is illustrated below.

Patent No. 3107820 discloses a method for producing a plant that has been rendered tolerant to cold injury by altering the fatty acid composition of lipids using a genetic recombination technique. Is disclosed. Japanese Patent Application Laid-Open No. 10-228983 discloses that a plant cell membrane-localized water channel protein gene is introduced into a plant to improve the function of maintaining the water potential of the plant. Methods for obtaining plants that are resistant to water stress such as loess or dry stress are disclosed. Japanese Patent Laid-Open Publication No. 2000-0 — 1166260 discloses that a gene for a transcription factor that controls a gene that acts to acquire resistance to drought, low temperature, or salt stress is cloned and stressed. A method has been disclosed for producing a plant in which drought, low temperature, or salt stress resistance is remarkably improved and dwarfing does not occur by introducing the gene linked downstream of a responsive promoter into a plant. _(In addition, Science 287, pp. 476-479, 2000 states that by using a genetic recombination technique to reduce the content of unsaturated fatty acids in the chloroplast membrane, it is possible to achieve excellent high-temperature adaptability. It is reported that the plant has been successfully produced, and the Journal of Plant Journal, Vol. 12, pp. 133-142, 1997 states that a synthase for glycine betaine, a substance that regulates osmotic pressure in plants. It has been reported that genes have been introduced to enhance salt tolerance. In addition, the creation of transgenic plants into which active oxygen scavenging enzymes have been introduced has been actively studied with the aim of creating complex environmental stress-tolerant plants (Protein Nucleic Acid Enzyme 44 vol. 2246— 2252, 1999).

However, all of these methods are methods for artificially imparting environmental stress resistance to plants by genetic recombination technology, and not only require efficient individual regeneration technology after introduction of the gene, but also Safety assessment of the effects of transgenic plants on the natural environment is necessary. According to the current safety evaluation guidelines, careful testing for a certain period of time is obligatory, and it will take a long time before practical use. It is also important to gain the public's understanding of the safety of using GMOs in food and everyday products.

The present invention has been made in view of the above circumstances, and has a much lower risk of imposing on the environment as compared with the prior art. The objective is to provide afforestation methods that enable afforestation in areas of high stress. Phytochemistry 方法 A method of regulating the plant's resistance to environmental stress from its original properties and regenerating the forest in areas with severe environmental stress is a simple industrial technology that contributes to the global environmental problem. This is a quick-acting method. Disclosure of the invention

Planting method of the present invention, by supplying the seedlings were planted ₂ dissolved water or o ₂ dissolved water co, a planting method that enables planting to harsh local environments be sampled less, the CO ₂ dissolved Water or O ₂ -dissolved water is supplied with CO ₂ or O ₂ in a pressurized state from the other side of the permeable membrane while passing raw water through one side separated by a permeable membrane that allows only gas to pass and liquid not to pass. supplying to a process produced the co ₂ or o ₂ is dissolved to a predetermined concentration with respect to the raw water.

A permeable membrane that allows only gas to pass but does not allow liquid to pass is typically a non-porous gas permeable membrane. A non-porous gas permeable membrane is a membrane through which gas is permeated by a dissolution and diffusion mechanism, and which does not substantially contain pores through which gas can permeate in a gaseous state like a Knudsen flow. . By using such a non-porous gas permeable membrane, gas can be supplied and dissolved at an arbitrary pressure without releasing the gas as bubbles, resulting in high efficiency. It can be easily dissolved at any concentration with good controllability.

The membrane form of the permeable membrane is a flat membrane, a tubular membrane, a hollow fiber membrane, a spiral membrane, or the like, and the hollow fiber membrane is more preferable because of a large membrane surface area. It is more preferably used because it is easier to handle and handle. The arrangement of the hollow fiber membranes is not particularly limited.However, knitting that maintains the intervals between the hollow fiber membranes at equal intervals, such as a braid, may cause channeling even in the flow of raw water or gas. composite membrane structure the structure of the hollow fiber membrane of less preferred _c nonporous is not particularly limited, for supporting and fixing the thin-film non-porous layer in the porous layer in order to increase the gas permeability of the non-porous membrane Is preferred. The composite membrane structure is also not particularly limited, but a composite hollow fiber membrane having a three-layer structure sandwiched between porous layers from both sides of a thin nonporous layer having excellent gas permeability is preferable because it protects the thin film layer.

As described above, the present invention uses a permeable membrane (preferably a hollow fiber membrane) that allows only gas to pass and does not allow liquid to pass, so that even if intermittent operation is performed, high-concentration CO ₂ Dissolved water or O ₂ dissolved water can be supplied to planted seedlings. That is, the required amount of dissolved gas water can be produced in the required amount and supplied to the planted seedlings.

As a method for supplying CO ₂ dissolved water or O ₂ dissolved water to planted seedlings, any method may be used. For example, an unglazed pipe is buried in the soil, gas-dissolved water is passed through the pipe, and the soil moisture is Underground rights irrigation, which absorbs as much water as necessary by means of water supply, is preferred. The underground irrigation method is a rational water supply method in arid regions.A water level control tank is installed between a pipe buried in the soil and a water supply tank that supplies gas-dissolved water. By setting the pipe at a position lower than the water level of the pipe, the water absorption power in the soil works and the gas-dissolved water gradually seeps out of the pipe. When the soil absorbs enough water, the water supply from the pipe stops, Do not overdo the gas-dissolved water. Is not particularly limited to the pipe material, but material permeable material has a permeability of water coefficient of ^{1 X 1 0- 6 ~ 1 X} 1 0- 3 c mZ s is preferred.

The concentration of co ₂ dissolved water and o ₂ dissolved water is more than the saturation concentration of the gas partial pressure of the gas occupying the gas phase in the soil in the air or plantations is preferred. The saturated solubilities of CO ₂ and O ₂ at 25 ° C and atmospheric gas partial pressure are about 0.5 ppm and 8. O ppm, respectively.

In addition, the planting method of the present invention uses a substance having an effect of inhibiting the action of cytochrome P450 in a concentration range that does not affect the normal growth of the seedlings above or below the planting seedlings. Or the ability to absorb into the body from both or both, or by absorbing it into the seeds of planting seedlings and then sowing and growing it, so that the plant's resistance to environmental stress is increased beyond its original properties, and this cytochrome P This is a tree-planting method that allows planting in areas with severe environmental stress by planting tree-planting seedlings that have been treated with substances that inhibit the function of 450.

Cytochrome P450 is a generic name for a group of protehem-containing proteins that are widely distributed in the biological world, bind to carbon monoxide in a reduced form, and exhibit a Soret absorption band near 450 nm. More than one species is known. Both act as monooxygenases that use two electrons from NAD (P) H and molecular oxygen to oxygenate lipophilic substrates. Its metabolic functions are diverse and are particularly involved in the biosynthesis of plant hormones such as sterols and brassinosterid gibberellins, oxidation of fatty acids ω, hydroxylation of flavonoids and lignin precursors (monolignol). I have. By using such a substance that inhibits the function of cytochrome 450, all or a part of various metabolic functions can be easily and simply inhibited to any extent. Substances that inhibit the function of cytochrome P450 are not particularly limited, but include ancimidol, flunoreprimidone, niconicozone P, and c. Kleb Trazonore, Brass Nazonore, Inabenfide, Tetocyclasis, Triapentenol, BAS 1 1 .1 W, l_n—Desilui Midazono, 1-Geraninolai Midazonole, HOE 0 7 4 7 8 4, Tria dime Knol, triadimefon, ipconazole. Teebconazole, metconazole, and the like are known, and it is preferable to use any selected from these. Generic name Park-mouth butrazole, a kind of plant growth regulator, Compound name (R *, R *)-(Sat)-β-[(4-chlorophenyl) -methyl-α- (1, 1-dimension (Tinoleethyl) 1 Η— 1,2,4-triazole-1 ethanol; (2RS, 3RS) —1— (4-cyclohexyl) -4,4-dimethyl-1 2- (1H-1,2,4-triazono 1-yl) pentane-3-ol is preferably used because of its versatility as a pesticide and ease of handling. Its properties, trade names, pesticide characteristics, mode of action, agricultural usage, and the like are described in detail in Japanese Patent Application Laid-Open No. 2001-231355.

As a method of absorbing the substance having the action of inhibiting the action of cytochrome P450 into the planting seedlings, a method of spraying a solution onto the above-ground portion (foliage) of the seedling or a method of absorbing the basement of the seedling (root portion) ) Or a method of soil treatment with granules, or a combination of both, or any method selected from a method of absorbing the solution into seeds of planting seedlings and then sowing and growing them. .

It is preferable that the concentration of the substance having an action of inhibiting the action of cytochrome P450 be absorbed by the planting seedlings in a concentration range that does not affect the normal growth of the plant. This is the working of the cytochrome P450 used It depends on the type of substance that has an inhibitory effect.

Furthermore, the tree planting method of the present invention is characterized in that a substance that inhibits the action of cytochrome P450 is absorbed into the body to plant a seedling with increased resistance to environmental stress from its original property, and then the CO ₍₂ ) It is characterized by a tree planting method that enables planting in areas with severe environmental stress by supplying dissolved water or O ₂ dissolved water to planted seedlings. BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 schematically illustrates a method for planting trees in an arid land according to a first embodiment of the present invention.

FIG. 2 illustrates a hollow fiber membrane module. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the tree planting method of the present invention will be described with reference to the drawings. ₍ FIG. 1 is a drawing illustrating a system configuration of a tree planting system for realizing the tree planting method of the present invention. P 4 5 ◦ and seedling PL for plantations to absorb a substance that inhibits the resistance to environmental be sampled less elevated even Ri by inherent properties of the action of the membrane module for manufacturing the CO ₂ dissolved water MO When the pipe PI unglazed buried in soil for feedwater C_〇 ₂ dissolved water, a water supply tank T for storing the CO ₂ dissolved water, and a pressure tank B Ombe for storing C 0 _2, the first and not including the second pressurizing pump P l, and P 2, and Tenshiometa one S 1 for measuring the soil moisture status, C 0 ₂ concentration centrally a measuring electrode S 2 for detecting the, open closed V The entire system is controlled by the control system including the various control valves and pressure gauges shown. Operation is control of.

Substances that inhibit the function of cytochrome P450 are not particularly limited Not, but Ansimidor, Funorel Primidor, Nikonazonore-I-P, Noclob Trazonore, Brassazonore, Inabenfide, Tetocyclasis, Triapenthenore, BAS 111.W, 111n-decylimidazole, 1 Monogeranyl imidazole, HOE 0 7 4 7 8 4, triadimenol, triadimefon, ipconazole, tebuconazole, metconazole, etc. are known, and it is preferable to use any selected from these. . Generic name Park-mouth butrazole, a kind of plant growth regulator, Compound name (R *, R *) — (Sat) — β — [(4-Chlorofluoro-norre) 1-methyl-α— (1, 1-dimethylethyl) ) 1 Η— 1,2,4-triazole 1 1 _ ethanol ノ; (2 RS, 3 RS) — 1 （1 一 (4 クロ 1 口 phenyl) 1 4 4, 4 メチルH-1,2,4-triazole-1-yl) Pentan-1-ol is a compound that is used more favorably because of its versatility and ease of handling as a pesticide, but is a similar compound. Pesticides containing P, ancimidol, flurbulimidole, and the like as main components can also be used.

The seedling PL for planting is not particularly limited, but both Eucalyptus spp. And Acacia spp. Are Oceania, mainly in Australia. It is a multi-species plant that grows naturally in the area, and many of the eucalyptus and acacia plants have excellent growth potential, are adaptable to various environments, and industrially produce timber and pulpwood. However, since it is suitable for the production of firewood and charcoal, it is preferable because it is actively planted in various parts of the world for early greening.

The planting seedlings PL may be in the form of potted seedlings or openly planted seedlings. Not only seedlings but also cuttings, grafts, tissue cultures, etc. Any of the cloned seedlings obtained by the method may be used, but in order to absorb and grow substances that inhibit the action of cytochrome P450, potted seedlings should be used in consideration of their spread to the environment. preferable.

A method for pre-absorbing substances that inhibit the function of cytochrome P450 into planting seedlings PL is to grow planting seedlings with granules or compound fertilizer. There is a method of irrigating the soil for growing seedlings, and a method of spraying a wettable powder on the foliage of planting seedlings. As for the time of absorption, it is preferable to absorb the seedlings for planting in a nursery before planting the planting seedlings PL, and it is preferable to absorb the seedlings from the beginning of the growth of the seedlings. Furthermore, a method in which the wettable powder is absorbed into the seeds of the planting seedlings and then sown and grown is used simply and preferably.

The amount of the substance that inhibits the action of cytochrome P450 can be absorbed by the planting seedlings PL depending on the chemicals used, and cannot be specified without any limitation. Good results can be obtained according to the amount used for elongation suppression (dwarfing). Planting seedlings that have absorbed a substance that inhibits the function of cytochrome P450 are usually dwarfed after their growth is suppressed after treatment. More specifically, it shows morphological changes such as shorter internodes, smaller leaves, and darker leaves. Seedlings that have undergone such morphological changes are more resistant to environmental stress than their original nature and are suitable for planting in areas with severe environmental stress. It is.

Water tank T is a container raw water for the production of CO ₂ dissolved water is automatically fed water. The type of raw water is not particularly limited, but groundwater, rainwater, and tap water pumped up by wells in consideration of cost are preferably used. These are particles that clog the membrane module MO with filters and ion exchangers, contaminating particles (microorganisms), and gold. It is advisable to remove the group ions in advance.

The pressurizing pump P 1 is used for supplying water in the water supply tank T to the membrane module MO. The flow rate and flow rate of water are determined by the discharge capacity of the pressure pump P1, and in the present apparatus, it is preferable to supply a flow rate of 1 to 15 liters / minute.

As shown in Fig. 1, the water discharged from the membrane module MO is returned to the water supply tank T. If the treated water is always returned, high-concentration gas-dissolved water can be easily generated by circulation of the treated water.

Membrane module MO, while passed through the raw water sent from the upstream, by supplying the C 0 ₂ gas is used as the Kyukimaku module for producing co ₂ dissolved water. It is preferable that the membrane module MO is formed by bundling thousands to tens of thousands of hollow fiber membranes and placing the bundles in a support case. In addition, the membrane module MO has a feature that it has a long life under ordinary use conditions and requires little maintenance.

As shown in Fig. 2 (a), the hollow fiber membrane 3 that constitutes the membrane module MO is formed into a cylindrical shape by sandwiching the nonporous membrane 4 between the porous membranes 5 and 5 in a sandwich shape, and is formed into a three-layer composite. Those having a film structure are preferred. This non-porous membrane 4 has selective gas permeability while blocking water permeation. For this reason, when gas is pressurized from the outside of the hollow fiber membrane 3, the gas can be mixed into the water inside the hollow fiber membrane 3. Therefore, in this apparatus, as shown in FIG. 2 (b), while passed through the water to the inside of the hollow fiber membrane 3, by supplying under pressure of CO ₂ from the outside of the hollow fiber membrane 3, CO ₂ Manufactures dissolved water. However, while water was passed water to the outside of the hollow fiber membrane 3, C 0 ₂ from the inside of the hollow fiber membrane 3 is supplied while pressurizing the C 0 ₂ Dissolved water may be produced.

Anyway, in the present apparatus, Ri by that create a pressure gradient inside and outside the hollow fiber membrane 3, the transmission efficiency of C_〇 ₂ for the hollow fiber membrane 3 is very high, any relative raw water the co ₂ dissolved water obtained by dissolving to a concentration can be easily manufactured. To this end, the gas pressure is set to 0.5 to 2. O kgf Zcm ² higher than the inside of the hollow fiber membrane 3 by using a control valve (not shown) provided between the membrane module MO and the CO ₂ gas cylinder. It may be set to a higher level.

The size of the CO ₂ gas cylinder can be selected and used according to the supply amount of CO ₂ dissolved water. Is a C 0 ₂ of the source also is not limited to base Gasubon, even with CO ₂ emitted by burning fossil fuels or the like in the air and factories selectively separated and recovered good.

C_〇 ₂ dissolved water produced through the membrane module M_〇 is becomes even higher Ri by the CO ₂ concentration dissolved in water at atmospheric conditions. And an example, 2 5 ° C, the saturation solubility of CO ₂ in the CO ₂ partial pressure existing in the atmosphere is about 0. 5 mg C 0 ₂ Zl l H ₂ 0. On the other hand, according to the present apparatus, by applying a gas pressure (1.0 kgf Z cm ² ) to the membrane module at 25 ° C, about 1.5 g C〇 ₂ // liter CO ₂ can be dissolved up to H ₂₀ . The Conoco The same applies to the case of dissolving the 0 _{2, 2} 5 ° C, the saturation solubility of O ₂ in the O ₂ partial pressure present in the atmosphere, about 8. 0 mg O ₂ / l Li Tsu torr H ₂ O. On the other hand, according to the present apparatus, by applying a gas pressure (1.0 kgf / cm ² ) to the membrane module at 25 ° C, about 40 mg O ₂ / l liter H ₂ O ₂ can be dissolved up to O.

In order to produce CO ₂ -dissolved water that has been dissolved to an arbitrary concentration in raw water, the CO ₂ electrode S 2 is immersed in the water supply tank T, and this electrode S 2 Luo CO ₂ dissolved concentrations proportional to the electrodes generated electromotive force (m V) to the control unit (not shown) as a signal Te cowpea to control the operation of the pressurizing pump P 1及Pi regulating valve V easily Can be achieved. This is the same when dissolving O _2, may be used by immersing the DO (di ssolved oxygen) electrode in place of the C 0 ₂ electrode in the water supply tank T.

Although the case of supplying CO ₂ dissolved water is shown in FIG. 1 above, the O ₂ dissolved water may be supplied by the same mechanism, and further, the CO ₂ dissolved water and the O ₂ dissolved water are switched. You may do it.

The form of supplying gas-dissolved water to the planted seedlings is not particularly limited, but an unfired pipe is buried in the soil, gas-dissolved water is passed through it, and only the water-supply capacity of the soil The method of absorbing water (underground irrigation) is preferred. According to this method, it is possible to supply rationally planted seedlings without wasting limited water in arid regions and gas-dissolved water produced using the water.

Specifically, as shown by the broken line in Fig. 1, a water level control tank is installed between the pipe PI buried in the soil and the water supply tank T that supplies gas-dissolved water. By setting the pipe lower than the water surface, the water absorption power in the soil works and the gas-dissolved water gradually leaks out of the pipe. Then, when the soil absorbs enough water, the water supply from the pipe stops and the gas-dissolved water is not overdosed. Preferably biscuit is a pipes material, tube material permeable material has a permeability of ^{1 X 1 0- 6 ~ 1 X} 1 0- 3 cm / s is preferred.

On the other hand, it is not necessary to provide a water level adjustment tank.In this case, the pressurizing pump P 2 shown in FIG. 1 supplies the water in the water supply tank T to the unglazed pipe PI buried in the soil. Used for The flow rate and flow rate of water are determined by the discharge capacity of the pressure pump P2. In this case, it is preferable to supply a flow rate of 20 to 500 milliliters Z.

As shown in Fig. 1, gas-dissolved water supplied from the water supply tank T is stored in the pipe because the outlet of the pipe PI is stopped, and gas is dissolved from the pipe by the water permeability of the pipe material. Water can gradually seep into the surrounding soil.

In order to rinse the gas-dissolved water according to the moisture condition of the surrounding soil, a tensiometer S1 is installed in the plantation to measure the capillary potential of the soil, and output from this sensor S1 according to the measured value. This can be easily achieved by controlling the operation of the pressurizing pump P2 by a control unit (not shown) in accordance with the signal given.

Here, the effect indicating the co ₂ dissolved water and o ₂ dissolved water is believed to cormorants good follows. In areas with severe environmental stress such as drying, the stomata of planted seedlings is closed. Then no longer be gas exchange, decreases the efficiency of photosynthesis and respiration lowers the C 0 ₂ concentration and 0 ₂ concentrations of plants. When supplying the seedlings planted co ₂ dissolved water及Pi o ₂ dissolved water, co ₂ and o ₂ are missing in plants is taken into the plant. As a result, the co ₂ concentration and the 〇 ₂ concentration in the plant body are increased, and photosynthesis and respiration are maintained, thereby enabling afforestation in an area where environmental stress is severe.

In the photosynthetic及Pi light respiratory reactions present in a plant, to the ribulose bis-phosphate carboxylase is carbonic fixed enzyme, o ₂ and co ₂ are competitive with the substrate, involving other substrates to each other Inhibits the reaction. That is, light respiration is large when a plant receives light under a gas phase condition of a high o ₂ concentration, and depending on the plant species, may be a factor that lowers the production by photosynthesis. Therefore, when the O ₂ dissolved water is supplied to the plant body is preferably a method for the dark period not exposed to light to switch between the C 0 ₂ dissolved water ヽ o

In addition, the effect of the substance that inhibits the action of cytochrome P450 on enhancing resistance to environmental stress is considered as follows. The activity of cytochrome P450 in planted seedlings is suppressed. Some of them are involved in the biosynthesis and biodegradation of plant hormones, which reduces the concentration of various plant hormones in the plant. The signal is used as a signal to constantly exert a defense mechanism against environmental stress in plants. In this way, the tolerance of the planted seedlings to environmental stress is higher than it should be, and planting such seedlings makes it possible to plant trees in areas with severe environmental stress.

(Example)

Hereinafter, the afforestation method of the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

(Example 1)

Using the equipment shown in Fig. 1 and the following plants and conditions, an experiment was conducted assuming a method of planting trees in arid land.

(1) Apparatus: The experiment was performed in a glass greenhouse where the temperature (20 to 26 ° C) can be controlled and the rainwater does not hit. The experimental sand tank used was 90 cm × 300 cm × 80 cm made of steel. The sand collected from the Tottori Sand Dunes was thoroughly washed with water, dried in the sun and sieved with a 2 mm sieve, filled into an experimental sand tank, and the sand was hydrated at a rate of 3 (w / w)%. An unglazed tube with an outer diameter of 50 mm and an inner diameter of 4 O mm is buried at an interval of 20 cm at a location of about 10 cm underground, and connected to a water supply tank with a rubber pipe to provide water (control area) and gas. Dissolved water (experimental section) was passed. At the center of the sand tank, a tensiometer was installed at a depth of 15 cm to output the soil moisture value, and the operation of the pump P2 reduced the pF value of the soil to approximately 3.8 to 4.0. So that it becomes I controlled. A carbon dioxide gas electrode and a DO electrode are immersed in the 100 L water tank, and the dissolved carbon dioxide value is output to control the operation of the control valve provided between the pump P1 and the gas cylinder and the membrane module. Te, make approximately 8 0 0 ppm CO ₂ dissolved water及Pi 2 0 ppm of 0 ₂ dissolved water in the water supply tank, and subjected to experiments.

(2) Plant

For the plants, cloned seedlings of Eucalyptus caraaldulensis cuttings were used. Implanted in a cutting, transplanted into a pot with a diameter of 9 cm and a depth of 12 cm after rooting, and immediately afterwards a Smith Seven P solution (containing 0.025% of Yukonazo Iluo P, manufactured by Agros Co., Ltd., Ministry of Agriculture, Forestry and Fisheries) (Registration No. 18010) 1 ml was rinsed in potting soil and grown for one month (experimental plot). Seedlings that had not been treated with drugs were used for the control plot. After that, the plants were planted in the above-mentioned experimental sand tank at intervals of 20 cm, and the dry weight of the above-ground part and the underground part after growing for three months was measured, and the experimental plot and the control plot were compared.

Table 1 shows the results. The numerical values in the table indicate the average values of 20 seedlings treated in the same manner.

Although not shown in the table, the seedlings treated with ゥ Niconazole-P had a lower degree of withering, and some seedlings not treated with the chemical (control group) died on the way. However, when the gas-dissolved water was given, even the seedlings not treated with the chemical (control group) were less likely to wither. In addition, the experimental plot to which gas-dissolved water was applied tended to have a higher dry matter weight than the control plot to which water was supplied.

From the above results, by supplying the seedlings planted CO ₂ dissolved water or O ₂ dissolved water, dried be sampled less to reduce the, and the child to allow planting to severe local drying be sampled less Indicated. In addition, cytochrome P 4 5 0 Absorption of a substance that has the effect of inhibiting the activity of planting into the planting seedlings enhances the plant's resistance to dry stress from its original properties, and enhances the action of this cytochrome P450. It was shown that planting planting seedlings treated with interfering substances could enable planting in areas with severe dry stress. Furthermore, after planting shea cytochrome P 4 5 0 seedlings subjected to a substance in the process of inhibiting the action of, by supplying the seedlings planted C 0 ₂ dissolved water or o ₂ dissolved water, dried be sampled It has been shown that it is possible to plant trees in areas where loess is severe.

(Example 2)

Using the following plants and conditions, the experiment was conducted assuming a method of planting trees in a cold region.

(1) Plant

The plants used were cutting cloned seedlings of Eucalyptus camal dulens is. After rooting in a cutting, transplanted to a pot with a diameter of 9 cm and a depth of 12 cm immediately after rooting, and immediately afterwards green field wettable powder (containing 50% of flurbulimidole, manufactured by Shionogi & Co., Ltd., Agriculture, Forestry and Fisheries) (Ministry Registration No. 1731 16) 0.lg was laid on potting soil and grown for 4 months (experimental plot). Seedlings that had not been treated with a drug were used for the control. Mid-February. Trees were planted during the extremely cold season (minimum temperature: 12 ° C, maximum temperature: 10 ° C), and terrestrial signs were observed and evaluated 10 days, 20 days, and 30 days later. The judgment results were quantified as follows. No harm: 0 points, slight harm: 1 point, moderate harm: 2 points, severe damage (withering): 3 points. In addition, at the time of planting, at 10, 10, and 30 days after planting, to estimate the change in the amount of chlorophyll in the same leaf, the SPAD value, which is an index of the chlorophyll value, was measured using a chlorophyll meter (Minolta turtle). The measurement was performed using SPAD-502) manufactured by La Co., Ltd., and the experimental plot was compared with the control plot. Table 2 shows the results. The harmful symptoms in the table indicate the average value of eight seedlings treated in the same manner, and the SPAD value indicates the average value of the values measured in five places on the same leaf of eight seedlings treated in the same manner. I have.

Based on the above results, absorption of a substance having an inhibitory effect on the action of cytochrome P450 into planting seedlings can enhance the plant's resistance to low-temperature environmental stress from its original properties. By planting planting seedlings that have been treated with a substance that inhibits the action of cytochrome P450, it is possible to plant trees in areas with severe low-temperature environmental stress It was shown that.

(Example 3)

Using the following plants and conditions, the experiment was conducted assuming a method of planting trees in a hot summer area.

(1) Plant

Seedlings of Eucalyptus camaldulensis were used as plants. Seed diluted for 1 day with Bonza solution (2% by weight of butrazole, manufactured by Takeda Pharmaceutical Co., Ltd., registered with the Ministry of Agriculture, Forestry and Fisheries, No. 17235) and immersed in lOOppb aqueous solution for 1 day The seeds were then sown and grown for 4 months (experimental plot). Seedlings that had not been treated with a drug were used for the control group. Trees were planted after leaving for 6 hours under the conditions of a temperature of 50 ° C and a light intensity of 10, 001 uX, and after 3 months, the length of withering with respect to the seedling height was measured.

Table 3 shows the results. The numerical values in the table are the average values of 10 trees with the withering rate (%). In addition, withering rate (%) = (length of withering in 3 months after treatment at 50 ° C for 6 hours) ÷ (height of seedling before treatment) X 100.

Based on the above results, a substance that has the effect of inhibiting the action of cytochrome P450 is absorbed into the seeds of planting seedlings and then sown and grown. By increasing the plant's resistance to high-temperature environmental stress from its original properties, and by planting plantation seedlings treated with a substance that inhibits the action of cytochrome P450. However, it was shown that it would be possible to plant trees in areas with high stress on high-temperature environments.

Conventionally, in the case of planting trees in areas with severe environmental stress, the environmental conditions are too severe, so that even with the diverse environmental adaptability of eucalyptus plants and acacia plants, they can be made to survive. There were areas where it was difficult to grow forests. As a result, there is an enormous area of land where tree planting has to be abandoned, and there has been a limit in using tree planting to reduce the ever-increasing carbon dioxide on the planet. A variety of plants, such as eucalyptus plants and acacia plants, are given environmental stress resistance (salt tolerance, drought tolerance, cold tolerance, heat resistance, etc.). If greening of cold and extremely hot regions can be done early to help reduce the ever-increasing amount of carbon dioxide on the earth, the benefits for humankind will be immense and the effect will be enormous.

However, we have created plants that are resistant to various environmental stresses by genetic recombination technology, and use these plants to green dry areas, salt-damaged areas, cold areas, and intensely hot places, and reduce the ever-increasing amount of carbon dioxide on the planet. In addition to the need for efficient individual regeneration technology after gene transfer, there are many barriers in terms of safety assessment such as the impact of transgenic plants on the natural environment. It exists and is expected to take a long time to reach practical use. Industrial applicability

As described above, according to the tree planting method of the present invention, a method that enables tree planting in an area where the environmental stress is severe compared to the conventional method can be performed quickly and safely. It can be provided efficiently.

【table 1】

[Table 2]

[Table 3]

Withering rate (%) Control plot (no drug treatment) 6 8 Experimental plot (Pak mouth butrazole treatment) 5

Claims

The scope of the claims

1. Co ₂ dissolved water or o ₂ to supply the seedlings were planted dissolved water, afforestation method to allow the planting of the severe regional ring Sakaisu door-less.

2. CO ₂ dissolved water or O ₂ by supplying the seedlings planted dissolved water, a plantation method that enables planting to severe local ring Sakaisu bets less, before Symbol CO ₂ dissolved water or O ₂ dissolved water, while passed through the raw water on one side the liquid is passed separated by the permeable membrane has a passed only gas, supplying CO ₂ or 〇 ₂ from the other side under pressure of the permeable membrane The method according to claim 1, wherein the CO ₂ or CO ₂ is produced by dissolving the CO ₂ or 〇 ₂ in the raw water to a predetermined concentration.

3. Absorb a substance that inhibits the action of cytochrome P450 in the body from above and / or below the planting seedlings in a concentration range that does not affect normal growth. By increasing the plant's resistance to environmental stress above its original level and planting plantation seedlings that have been treated with a substance that inhibits the function of cytochrome P450, Afforestation method that enables afforestation in areas with severe stress.

4. In a concentration range that does not affect normal growth, a substance that has the effect of inhibiting the action of cytochrome P450 is absorbed into seeds of planting seedlings, and then sown and grown. Planting plantation seedlings that have been treated with substances that enhance the resistance of plants to environmental stress beyond their inherent properties and that inhibits the function of cytochrome P450 will lead to strict environmental stress. Tree planting method that enables tree planting in new areas.

5. CO ₂ dissolved water or O ₂ by supplying the seedlings planted dissolved water, a plantation method that enables planting to severe local ring Sakaisu bets less, seedlings of pre Symbol plantation, cytochrome Treat with a substance that inhibits the action of P450 The planting method according to any one of claims 1 to 4, wherein the planting is performed.

6. Substances that have the effect of inhibiting the function of cytochrome P450 are ancimidonore, funorenoreprimimidonore, zekonazonole p, cloclobutrazole, brassinazo Λ ^, inavenbenido, tetocyclasis, tri Apentenol, BAS 1 1 1. W, selected from 1-n-decyl imidazole, 1-geranyl imidazole, HO E 0 7 4 7 8 4, triadimenol, triadimefone, ipconazole, tebuconazole and metconazole The planting method according to claim 3, wherein the planting method is any one of: