WO2003097780A1 - Enzymatic detergent compositions - Google Patents
Enzymatic detergent compositions Download PDFInfo
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- WO2003097780A1 WO2003097780A1 PCT/EP2003/004743 EP0304743W WO03097780A1 WO 2003097780 A1 WO2003097780 A1 WO 2003097780A1 EP 0304743 W EP0304743 W EP 0304743W WO 03097780 A1 WO03097780 A1 WO 03097780A1
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- pyridin
- detergent composition
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/26—Organic compounds containing nitrogen
- C11D3/28—Heterocyclic compounds containing nitrogen in the ring
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/39—Organic or inorganic per-compounds
- C11D3/3902—Organic or inorganic per-compounds combined with specific additives
- C11D3/3905—Bleach activators or bleach catalysts
- C11D3/3932—Inorganic compounds or complexes
Definitions
- EP-A-214 761 discloses lipases which are derived from organisms of the species Pseudomonas cepacia
- EP-A-258 068 discloses lipases which are derived from organisms of the genus Humicola . Both patent applications also describe the use of these lipases as detergent additives.
- Further examples of lipase-containing detergent compositions are provided by EP-A-205 208 and EP-A-206 390 (both Unilever) , which disclose a class of lipases defined on the basis of their immunological relationships, and describe their use in detergent compositions and textile washing.
- the preferred lipases are those from Pseudomonas fluorescens, Pseudomonas gladioli and Chromobacter species.
- EP-A-331 376 (Amano) describes lipases, their use and their production by means of recombinant DNA (rDNA) techniques, and includes an amino acid sequence of lipase from Pseudomonas cepacia . Further examples of lipase enzymes produced by means of rDNA techniques are given in WO-A-89/09263 and EP-A-218 272 (both Gist-Brocades) .
- Lipolase is no exception. During the drying process, when the water content of the fabric is reduced, the enzyme regains its activity and the fatty stains are hydrolysed. During the following wash cycle the hydrolysed material is removed. This also explains why the effect of lipases is low after the first washing cycle, but significant in the following cycles.
- lipolytic enzymes or lipases can synergistically interact with certain transition metal bleach catalysts to provide superior cleaning performance to detergent compositions containing them.
- an enzymatic detergent composition comprising:
- the preferred detergent-active compounds that can be used are soaps and synthetic non-soap anionic and nonionic compounds.
- Anionic surfactants are well-known to those skilled in the art. Examples include alkylbenzene sulphonates, particularly linear alkylbenzene sulphonates having an alkyl chain length of C 8 -C ⁇ 5 ; primary and secondary alkylsulphates, particularly C 8 -C 15 primary alkyl sulphates; alkyl ether sulphates; olefin sulphonates; alkyl xylene sulphonates; dialkyl sulpho-succinates; and fatty acid ester sulphonates.
- Sodium salts are generally preferred.
- detergent-active compound surfactant
- amount present will depend on the intended use of the detergent composition.
- surfactant systems may be chosen, as is well known to the skilled formulator, for handwashing products and for products intended for use in different types of washing machine.
- the total amount of surfactant present will also depend on the intended end use and may be as high as 60% by weight, for example, in a composition for washing fabrics by hand. In compositions for machine washing of fabrics, an amount of from 5 to 40% by weight is generally appropriate.
- Detergent compositions suitable for use in most automatic fabric washing machines generally contain anionic non-soap surfactant, or nonionic surfactant, or combinations of the two in any ratio, optionally together with soap. Also applicable are surfactants such as those described in EP-A-328 177 (Unilever) , which show resistance to salting-out, the alkyl polyglycoside surfactants described in EP-A-070 074, and alkyl monoglycosides .
- Preferred surfactant systems are mixtures of anionic with nonionic detergent active materials, in particular the groups and examples of anionic and nonionic surfactants pointed out in EP-A-346 995 (Unilever) .
- surfactant system which is a mixture of an alkali metal salt of a Ci6-C ⁇ 8 primary alcohol sulphate together with a C ⁇ 2 -C 15 primary alcohol 3-7 EO ethoxylate.
- the enzymatic detergent compositions of the invention comprise 10 - 20,000 LU per gram of the detergent composition of a lipolytic enzyme selected from the group consisting of Lipolase, Lipolase ultra, LipoPrime, Lipomax, Liposam, Lipex and lipase from Rhizomucor miehei (e.g. as described in EP-A-238 023 (Novo Nordisk) .
- a lipolytic enzyme selected from the group consisting of Lipolase, Lipolase ultra, LipoPrime, Lipomax, Liposam, Lipex and lipase from Rhizomucor miehei (e.g. as described in EP-A-238 023 (Novo Nordisk) .
- a further method of assessing the enzymatic activity is by measuring the reflectance at 460 nm according to standard techniques .
- Suitable enzymes for the compositions of the invention can be found in the enzyme classes of the esterases and lipases, (EC 3.1.1.*, wherein the asterisk denotes any number) .
- Cutinases as lipolytic enzymes which exhibit substantially no interfacial activation. Cutinases therefor differ from classical lipases in that they do not possess a helical lid covering the catalytic binding site. Cutinases belong to a different subclass of enzymes (EC 3.1.1.50) and are regarded to be outside the scope of the present invention. Of main interest for the present invention are fungal lipases, such as those from Humicola lanuginosa and Rhizomucor miehei .
- the lipolytic enzyme of the present invention can usefully be added to the detergent composition in any suitable form, i.e. the form of a granular composition, a slurry of the enzyme, or with carrier material (e.g. as in EP-A-258 068 and the Savinase (TM) and Lipolase (TM) products of Novozymes) .
- carrier material e.g. as in EP-A-258 068 and the Savinase (TM) and Lipolase (TM) products of Novozymes
- a good way of adding the enzyme to a liquid detergent product is in the form of a slurry containing 0.5 to 50 % by weight of the enzyme in a ethoxylated alcohol nonionic surfactant, such as described in EP-A-450 702 (Unilever) .
- the enzyme to be used in the detergent compositions according to the invention can be produced by cloning the gene for the enzyme into a suitable production organism, such as Bacilli , or Pseudomonaceae, yeasts, such as Saccharomyces, Kluyveromyces, Hansenula or Pichia, or fungi like Aspergillus .
- a suitable production organism such as Bacilli , or Pseudomonaceae, yeasts, such as Saccharomyces, Kluyveromyces, Hansenula or Pichia, or fungi like Aspergillus .
- the preferred production organism is Aspergillus with especial preference for Aspergillus oryzae .
- the enzymatic detergent compositons of the invention comprise a bleach catalyst, which is a complex of a transition metal and a polydentate nitrogen donor ligand excluding cross-bridged macrocyclic ligands.
- the bleach catalyst per se may be selected from a wide range of organic molecules (ligands) and complexes thereof.
- Suitable organic molecules (ligands) and complexes for use with an oxygen solution boosting agent are found, for example in: GB 9906474.3; GB 9907714.1; GB 98309168.7, GB 98309169.5; GB 9027415.0 and GB 9907713.3; DE-A-19755493 ; EP-A-999050; WO-A- 9534628; EP-A-458379; EP-A-909809; US-A-4 , 728 , 455 ; WO-A- 98/39098; WO-A-98/39406 , WO-A-9748787 , WO-A-00/29537 and WO-A- 00/52124, the complexes and organic molecule (ligand) precursors of which are herein incorporated by reference.
- N-methyl-N,N' ,N' -tris (3 -methyl-pyridin-2-ylmethyl) ethylene-1, 2- diamine
- N-benzyl-N, N' ,N' -tris (3-methyl-pyridin-2- ylmethyl) ethylene-1, 2 -diamine
- N-methyl-N, ' , ' -tris (pyridin-2- ylmethyl) ethylene-1, 2-diamine
- N-benzyl-N, N' ,N' -tris (pyridin-2- ylmethyl) ethylene-1, 2 -diamine
- N,N,N' ,N' -tetrakis (3 -methyl- pyridin-2-
- the selection of the counter ion Y for establishing charge neutrality is not critical for the activity of the complex.
- said counter ions are chloride, sulphate, nitrate, methylsulphate, surfactant-ions, such as long chain alkylsulphates, alkylsulphonates, alkylbenzenesulphonates, tosylate, trifluoromethylsulphonate, perchlorate, BPh4-, PF6-, and mixtures thereof.
- the non-exhaustive list is: tris (pyridin-2-ylmethyl) amine; 1,4, 7-tris (pyrazol-1-ylmethyl) -1,4, 7-triazacyclononane; 1,4- bis (quinolin-2-ylmethyl) -7-ethyl-l, 4 , 7-triazacyclononane; 1,1- bis (pyridin-2-yl) -N-methyl-N- (pyridin-2-ylmethyl) methylamine; 1, 1-bis (pyridin-2-yl) -N,N-bis (6-methyl-pyridin-2- ylmethyl) methylamine; 2, 6-bis (pyridin-2-ylmethyl) -1,1,7,7- tetrakis (pyridin-2-yl) -2, 6-diazaheptane; 1, 1-bis (pyridin-2-yl) ⁇ 1-benzyl-N,N-bis (pyridin-2-ylmethyl) methylamine; 1,1- bis (
- N,N' ,N' -tris (3-methyl-pyridin-2-ylmethyl) -ethylenediamine; N- methyl-N,N' ,N' -tris (pyridin-2-ylmethyl) -ethylenediamine; N- methyl-N,N' ,N' -tris (5-ethyl-pyridin-2-ylmethyl) - ethylenediamine; N-methyl-N,N 1 ,N' -tris (5-methyl-pyridin-2- ylmethyl) -ethylenediamine; N-methyl-N,N' ,N' -tris (3-methyl- pyridin-2-ylmethyl) -ethylenediamine; N-benzyl-N,N' ,N' -tris (3- methyl-pyridin-2 -ylmethyl) -ethylenediamine; N,N,N" -tris (3- methyl-pyridin-2-ylmethyl) -ethylenediamine; N,
- Inorganic builders that may be present include sodium carbonate, if desired in combination with a crystallisation seed for calcium carbonate, as disclosed in GB-A-1 437 950 (Unilever) ; crystalline and amorphous aluminosilicates, for example, zeolites as disclosed in GB-A-1 473 201 (Henkel) , amorphous aluminosilicates as disclosed in GB-A-1 473 202 (Henkel) and mixed crystalline/amorphous aluminosilicates as disclosed in GB-A-1 470 250 (Procter & Gamble) ; and layered silicates as disclosed in EP-B-164 (Hacksawed) .
- Inorganic phosphate builders for example, sodium orthophosphate, pyrophosphate and tripolyphosphate, may also be present, but on environmental grounds those are no longer preferred.
- the detergent compositions of the invention preferably contain an alkali metal, preferably sodium, aluminosilicate builder.
- Sodium aluminosilicates may generally be incorporated in amounts of from 10 to 70% by weight (anhydrous basis) , preferably from 25 to 50% by weight.
- the alkali metal aluminosilicate may be either crystalline or amorphous or mixtures thereof, having the general formula: 0.8-1.5 Na 2 0. Al 2 0 3 . 0.8-6 Si0 2
- the zeolite may be the commercially available zeolite 4A now widely used in laundry detergent powders.
- the zeolite builder incorporated in the compositions of the invention is maximum aluminium zeolite P (zeolite MAP) as described and claimed in EP-A-384 070 (Unilever) .
- Zeolite MAP is defined as an alkali metal aluminosilicate of the zeolite P type having a silicon to aluminium ratio not exceeding 1.33, preferably within the range of from 0.90 to 1.33, and more preferably within the range of from 0.90 to 1.20.
- zeolite MAP having a silicon to aluminium ratio not exceeding 1.07, more preferably about 1.00.
- the calcium binding capacity of zeolite MAP is generally at least 150 mg CaO per g of anhydrous material .
- Organic builders that may be present include polycarboxylate polymers such as polyacrylates, acrylic/maleic copolymers, and acrylic phosphinates; monomeric polycarboxylates such as citrates, gluconates, oxydisuccinates, glycerol mono-, di- and trisuccinates, carboxymethyloxysuccinates , carboxymethyl-oxymalonates, dipicolinates, hydroxyethyl-iminodiacetates, alkyl- and alkenylmalonates and succinates; and sulphonated fatty acid salts.
- polycarboxylate polymers such as polyacrylates, acrylic/maleic copolymers, and acrylic phosphinates
- monomeric polycarboxylates such as citrates, gluconates, oxydisuccinates, glycerol mono-, di- and trisuccinate
- organic builders are citrates, suitably used in amounts of from 5 to 30% by weight, preferably from 10 to 25% by weight, and acrylic polymers, more especially acrylic/maleic copolymers, suitably used in amounts of from 0.5 to 15%, preferably from 1 to 10% by weight.
- Builders both inorganic and organic, are preferably present in the form of their alkali metal salt, especially their sodium salt.
- Detergent compositions according to the invention may additionally contain a conventional bleach system.
- Fabric washing compositions may desirably contain peroxy bleach compounds, for example, inorganic persalts or organic peroxyacids, capable of yielding hydrogen peroxide in aqueous solution.
- Suitable peroxy bleach compounds include organic peroxides such as urea peroxide, and inorganic persalts such as the alkali metal perborates, percarbonates, perphosphates, persilicates and persulphates.
- Preferred inorganic persalts are sodium perborate monohydrate and tetrahydrate, and sodium percarbonate.
- sodium percarbonate having a protective coating against destabilisation by moisture Sodium percarbonate having a protective coating comprising sodium metaborate and sodium silicate is disclosed in GB-A-2 123 044 (Kao) .
- the peroxy bleach compound is suitably present in an amount of from 5 to 35 wt%, preferably from 10 to 25 wt%.
- the bleach system may contain apart from the hydrogen peroxide source, as disclosed above, also a peracid-forming bleach activator or precursor to improve bleaching action at low wash temperatures.
- Preferred bleach precursors are peroxycarboxylic acid precursors, more especially peracetic acid precursors and peroxybenzoic acid precursors; and peroxycarbonic acid precursors.
- bleach activators such as tetraacetylethylene-diamine (TAED) or N,N- phthaloylaminoperoxy caproic acid (PAP) .
- the novel quaternary ammonium and phosphonium bleach precursors disclosed in US-A- 4 751 015 and US-A-4 818 426 (Lever Brothers Company) and EP-A- 402 971 (Unilever) are also of great interest.
- peroxycarbonic acid precursors in particular cholyl-4-- sulphophenyl carbonate can be used.
- peroxybenzoic acid precursors in particular, N,N,N- trimethylammonium toluoyloxy benzene sulphonate; and the cationic bleach precursors disclosed in EP-A-284 292*and EP-A- 303 520 (Kao) .
- the bleach precursor is suitably present in an amount of from 1 to 8 wt%, preferably from 2 to 5 wt%.
- bleach catalysts can be included.
- Such compounds are well known in the art and include, for example, manganese-based catalysts as disclosed in US-A-5 246 621, US-A- 5 244 594, US-A-5 194 416, US-A-5 114 606, EP-A-458 397 and EP- A-458 398 EP-A-509 787 or the iron-based catalysts as disclosed in WO-A-95/34628.
- a bleach stabilizer (heavy metal sequestrant) may also be present. Suitable bleach stabilizers include ethylenediamine tetraacetate (EDTA) and the polyphosphonates such as Dequest (Trade Mark), EDTMP.
- the bleaching detergent compositions of the present invention may additionally comprise one or more enzymes, which provide cleaning performance, fabric care and/or sanitation benefits.
- enzymes include oxidoreductases, transferases, hydrolases, lyases, isomerases and ligases. Suitable members of these enzyme classes are described in Enzyme nomenclature 1992: recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology on the nomenclature and classification of enzymes, 1992, ISBN 0- 12-227165-3, Academic Press.
- hydrolases are carboxylic ester hydrolase, thiolester hydrolase, phosphoric monoester hydrolase, and phosphoric diester hydrolase which act on the ester bond; glycosidase which acts on O-glycosyl compounds; glycosylase hydrolysing N-glycosyl compounds; thioether hydrolase which acts on the ether bond; and exopeptidases and endopeptidases which act on the peptide bond.
- carboxylic ester hydrolase, glycosidase and exo- and endopeptidases are examples of the hydrolases.
- suitable hydrolases include (1) exopeptidases such as aminopeptidase and carboxypeptidase A and B and endopeptidases such as pepsin, pepsin B, chymosin, trypsin, chymotrypsin, elastase, enteropeptidase, cathepsin B, papain, chymopapain, ficain, thrombin, plasmin, renin, subtilisin, aspergillopepsin, collagenase, clostripain, kallikrein, gastricsin, cathepsin D, bromelain, chymotrypsin C, urokinase, cucumisin, oryzin, proteinase K, thermomycolin, thermitase, lactocepin, thermolysin, bacillolysin.
- exopeptidases such as aminopeptidase and carboxypeptidase A and B and endopeptida
- carboxylic ester hydrolase including carboxylesterase, lipase, phospholipase, pectinesterase, cholesterol esterase, chlorophyllase, tannase and wax-ester hydrolase.
- transferases and ligases are glutathione S- transferase and acid-thiol ligase as described in WO-A-98/59028 and xyloglycan endotransglycosylase as described in WO-A- 98/38288.
- lyases examples include hyaluronate lyase, pectate lyase, chondroitinase, pectin lyase, alginase II.
- pectolyase which is a mixture of pectinase and pectin lyase .
- a process for enhancing the efficacy of the bleaching action of oxidoreductases is by targeting them to stains by using antibodies or antibody fragments as described in WO-A- 98/56885.
- Antibodies can also be added to control enzyme activity as described in WO-A-98/06812.
- Suitable amylases include those of bacterial or fungal origin. Chemically or genetically modified variants of these enzymes are included as described in WO-A-99/02632 pages 18,19.
- Commercial cellulase are sold under the tradename PurastarTM, Purastar OxAmTM (formerly Purafact Ox AmTM ) by Genencor;
- Detergent enzymes are usually incorporated in an amount of 0.00001% to 2%, and more preferably 0.001% to 0.5%, and even more preferably 0.01% to 0.2% in terms of pure enzyme protein by weight of the composition.
- Detergent enzymes are commonly employed in the form of granules made of crude enzyme alone or in combination with other components in the detergent composition. Granules of crude enzyme are used in such an amount that the pure enzyme is 0.001 to 50 weight percent in the granules. The granules are used in an amount of 0.002 to 20 and preferably 0.1 to 3 weight percent.
- Granular forms of detergent enzymes are known as EnzoguardTM granules, prills, marumes or T-granules.
- polymeric dye transfer inhibiting agents are normally incorporated into detergent compositions in order to inhibit the transfer of dyes from colored fabrics onto fabrics washed therewith. These polymers have the ability to complex or adsorb the fugitive dyes washed out of dyed fabrics before the dyes have the opportunity to become attached to other articles in the wash.
- polymeric dye transfer inhibiting agents are polyamine N-oxide polymers, copolymers of N- vinylpyrrolidone and N-vinylimidazole, polyvinylpyrrolidone polymers, polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof .
- Lipolase to boost the bleaching performance of various metal catalysts was assessed by washing cotton swatches soiled with tomato-oil stains
- Tomato/soy oil stained cloths were added and stirred for 30 minutes at 25 °C (blanks) in the following detergent composition dosed at 2 g/1 in Milli-Q water with 0.6 mM CaCl 2 added. Cloth to liquor ratio was 1:40. The pH of the wash solution was 10 at the start of the wash.
- Tris-buffer For the control wash without lipase a similar amount of the 10 mM Tris-buffer was added to avoid pH differences between wash solutions.
- the transition metal complexes were pre-dissolved in Milli-Q water or in mixtures of organic solvent (ethanol, methanol, dichloromethane) and water to a concentration of 2.25 mM. These stock solutions are diluted 30x with Milli-Q water and then another 15x when added to the wash solutions.
- ⁇ L is a measure for the difference in darkness between the washed and clean test cloth
- ⁇ a and ⁇ b are measures for the difference in redness and yellowness respectively between both cloths.
- CIE Commission International de l'Eclairage
- N,N-bis (pyridin-2 -yl-methyl) -1, 1-bis (pyridin-2-yl) -1- aminoethane, MeN4Py was prepared according to the procedure described in EP-A-909 809 (Unilever) .
- the ligand L (1H-1 , 4, 8, 11-Benzotetraazacyclotridecine- 2,5,7,10(6H,11H) tetrone, 13 , 14-dichloro-6, 6-diethyl-3 , 4 , 8, 9- tetrahydro-3 , 3, 9, 9-tetramethyl ) was synthesised as described in literature (T.J. Collins et al . , J. Am. Chem. Soc . (1991), 113(22), 8419-25).
- the iron complex was prepared as described elsewhere in WO-A-98/03625 (Carnegie-Mellon University) , using lithium salt as counter ion.
- Ligand 1, 4 , 7-triazacyclononane was produced according the modified method used by the team of Prof. Wieghardt. In this method the detosylation of the 1, 4, 7-tris-p-toluenesulfon- 1, 4 , 7-triazacylononanamide is performed in 5 minutes in hot sulphuric acid of 180°C. Once the solution has cooled down it is transferred into ether under vigorous stirring. The solution that surfaces is decanted and the residue is dissolved in some boiling water. At boiling temperature drops of concentrated hydrochloric acid are added. The brown crystals that precipitate are drained off and washed with cold hydrochloric acid and then with ethanol and ether. The 1,4,7- triazacyclononane . trihydrochloride thus produced is then processed further as described by Wieghardt et al (K. Wieghardt et al, Chem Ber., 112, 2200 (1979)).
- the quinolinemethylbromide is produced as follows. In this method 0.2 mol quinoline (30.0 g) with 0.22 mol N- bromsuccinimid (42 g) and dibenzoylperoxide as starter are placed in 300 ml freshly distilled benzene under irradiation of light. The succinimid that is sedimented after strong cooling is filtered off and the benzene is rotated off. The remaining oil is put into 5% hydrobromic acid. Under cooling with ice a saturated solution of sodiumcarbonate is added to the watery solution up to a pH-value of 7. The precipitated yellowish product is drained off and recrystallized from pentane.
- Detergent Composition Na-LAS 24.8 %
- Table 2 shows the Stain bleach performance of detergent with and without transition metal complexes and with and without lipase on curry oil stains.
- Table 2a Curry/oil stains on cotton (response after 3 days) . Stain residue (after wash) compared to clean cotton in ⁇ E.
- the bleaching of the tomato oil and curry oil stains is best if both a lipase and a metal complex are present.
- a synergistic effect is seen between stain removal by the lipase and the metal complex.
- the synergistic effect is larger for the fungal lipases such as L4384 (Rhizopus arrhizus) , LipoPrime, Lipolase ultra, Lipolase (all originating from Humicola lanuginosa) and L9031 (-Rhizomucor miehei) .
- L4384 Rhizopus arrhizus
- LipoPrime Lipolase ultra
- Lipolase all originating from Humicola lanuginosa
- L9031 -Rhizomucor miehei
- the initial pH of the detergent solution was 9.98. As is clear the pH of the wash solution drops in particular for the tomato oil stains. However, this drop cannot explain the marked synergy between the catalyst and the lipase. It is expected that the drop in pH is less for the other detergent formulations used in Examples 1 and 3.
- the detergent formulation that was used was as follows :
- the fabrics used were green or blue cotton stretch t-shirts (98% cotton, 2% lycra) . Each fabric was stained with various oils such as, olive oil, corn oil, artificial sebum, and hamburger grease, and washed in the respective products. Evaluation:
Abstract
Description
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Priority Applications (3)
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EP03725158A EP1506277A1 (en) | 2002-05-20 | 2003-05-07 | Enzymatic detergent compositions |
AU2003227725A AU2003227725A1 (en) | 2002-05-20 | 2003-05-07 | Enzymatic detergent compositions |
CA002483639A CA2483639A1 (en) | 2002-05-20 | 2003-05-07 | Enzymatic detergent compositions |
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US10/151,628 US20030050211A1 (en) | 2000-12-14 | 2002-05-20 | Enzymatic detergent compositions |
US10/151,628 | 2002-05-20 |
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WO2003097780A1 true WO2003097780A1 (en) | 2003-11-27 |
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EP (1) | EP1506277A1 (en) |
AU (1) | AU2003227725A1 (en) |
CA (1) | CA2483639A1 (en) |
WO (1) | WO2003097780A1 (en) |
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Cited By (3)
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EP1712610A1 (en) | 2005-02-22 | 2006-10-18 | The Procter and Gamble Company | Detergent Compositions |
WO2012084582A1 (en) * | 2010-12-21 | 2012-06-28 | Henkel Ag & Co. Kgaa | Liquid surfactant preparation containing lipase and phosphonate |
US8859480B2 (en) | 2005-02-22 | 2014-10-14 | The Procter & Gamble Company | Detergent compositions |
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GB0313253D0 (en) * | 2003-06-09 | 2003-07-16 | Unilever Plc | Bleaching composition |
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Also Published As
Publication number | Publication date |
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US20030050211A1 (en) | 2003-03-13 |
ZA200408278B (en) | 2005-10-13 |
EP1506277A1 (en) | 2005-02-16 |
AU2003227725A1 (en) | 2003-12-02 |
CA2483639A1 (en) | 2003-11-27 |
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