WO2003087821A3 - Method for cleaving and deglycosylating antibodies to promote ligand binding - Google Patents

Method for cleaving and deglycosylating antibodies to promote ligand binding Download PDF

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Publication number
WO2003087821A3
WO2003087821A3 PCT/US2003/010673 US0310673W WO03087821A3 WO 2003087821 A3 WO2003087821 A3 WO 2003087821A3 US 0310673 W US0310673 W US 0310673W WO 03087821 A3 WO03087821 A3 WO 03087821A3
Authority
WO
WIPO (PCT)
Prior art keywords
cleaving
antibody
deglycosylating
antibodies
methods
Prior art date
Application number
PCT/US2003/010673
Other languages
French (fr)
Other versions
WO2003087821A2 (en
Inventor
Lisa S Kelly Ph D
David J Puett
Michael J Pierce
Original Assignee
Univ Georgia Res Found
Lisa S Kelly Ph D
David J Puett
Michael J Pierce
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Georgia Res Found, Lisa S Kelly Ph D, David J Puett, Michael J Pierce filed Critical Univ Georgia Res Found
Priority to CA002481420A priority Critical patent/CA2481420A1/en
Priority to AU2003221671A priority patent/AU2003221671A1/en
Priority to EP03718249A priority patent/EP1495323A2/en
Publication of WO2003087821A2 publication Critical patent/WO2003087821A2/en
Publication of WO2003087821A3 publication Critical patent/WO2003087821A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
    • G01N2333/42—Lectins, e.g. concanavalin, phytohaemagglutinin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/02—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates involving antibodies to sugar part of glycoproteins

Abstract

The present invention details methods to detect glycoproteins in samples to identify a disease state. In particular, an improved sandwich assay is described, using both an antibody and an array of lectins. Methods for increasing the efficiency of interaction of an antibody with a ligand by cleaving the antibody to remove the carbohydrate moiety, and orienting the cleaved fragment on a matrix to enhance recognition of a ligand are also described. Methods of differentiating pregnancy-derived and disease derived samples are also described.
PCT/US2003/010673 2002-04-05 2003-04-07 Method for cleaving and deglycosylating antibodies to promote ligand binding WO2003087821A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002481420A CA2481420A1 (en) 2002-04-05 2003-04-07 Method for cleaving and deglycosylating antibodies to promote ligand binding
AU2003221671A AU2003221671A1 (en) 2002-04-05 2003-04-07 Method for cleaving and deglycosylating antibodies to promote ligand binding
EP03718249A EP1495323A2 (en) 2002-04-05 2003-04-07 Method for cleaving and deglycosylating antibodies to promote ligand binding

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37058902P 2002-04-05 2002-04-05
US60/370,589 2002-04-05

Publications (2)

Publication Number Publication Date
WO2003087821A2 WO2003087821A2 (en) 2003-10-23
WO2003087821A3 true WO2003087821A3 (en) 2004-08-19

Family

ID=29250552

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/010673 WO2003087821A2 (en) 2002-04-05 2003-04-07 Method for cleaving and deglycosylating antibodies to promote ligand binding

Country Status (4)

Country Link
EP (1) EP1495323A2 (en)
AU (1) AU2003221671A1 (en)
CA (1) CA2481420A1 (en)
WO (1) WO2003087821A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2404734A (en) * 2003-08-05 2005-02-09 Secr Defence A method of screening a sample for abnormally glycosylated and/or expressed proteins
US8632983B2 (en) 2005-04-15 2014-01-21 Van Andel Research Institute Biomarkers for pancreatic cancer and diagnostic methods
US7838634B2 (en) * 2005-04-15 2010-11-23 Van Andel Research Institute Methods for measuring glycan levels of proteins
US7879799B2 (en) 2006-08-10 2011-02-01 Institute For Systems Biology Methods for characterizing glycoproteins and generating antibodies for same
EP2037274A1 (en) * 2007-09-11 2009-03-18 GALAB Technologies GmbH Lectin based glycane assay
JP5924455B2 (en) 2013-09-09 2016-05-25 株式会社島津製作所 Peptide fragment preparation method, peptide fragment preparation kit used therefor, and analysis method
CN104678103A (en) * 2014-08-05 2015-06-03 首都医科大学附属北京佑安医院 Chemical luminescent protein chip, kit and detection method for detecting fucose index of seroglycoid
BR112017017854A2 (en) * 2015-03-09 2018-04-10 Shimadzu Corporation method for detecting monoclonal antibody using mass spectrometry
KR20170120178A (en) * 2015-03-10 2017-10-30 가부시키가이샤 시마즈세이사쿠쇼 Sample Preparation Kit for Detection of Monoclonal Antibody
US10753936B2 (en) 2016-07-22 2020-08-25 Van Andel Research Institute Method of detecting the level of a glycan
CA3230920A1 (en) * 2021-09-10 2023-03-16 Jason J. SUN Agglutinating agents, devices and methods for whole blood assays

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIRKEN S ET AL: "Immunochemical measurement of early pregnancy isoforms of HCG: potential applications to fertility research, prenatal diagnosis, and cancer.", ARCHIVES OF MEDICAL RESEARCH. UNITED STATES 2001 NOV-DEC, vol. 32, no. 6, November 2001 (2001-11-01), pages 635 - 643, XP002270855, ISSN: 0188-4409 *
ELLIOTT M M ET AL: "Carbohydrate and peptide structure of the alpha- and beta-subunits of human chorionic gonadotropin from normal and aberrant pregnancy and choriocarcinoma.", ENDOCRINE. UNITED STATES AUG 1997, vol. 7, no. 1, August 1997 (1997-08-01), pages 15 - 32, XP009026237, ISSN: 0969-711X *
KELLY LISA S ET AL: "A lectin-based ELISA assay to distinguish human chorionic gonadotropin produced during pregnancy and malignancy", PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH ANNUAL, vol. 43, March 2002 (2002-03-01), 93rd Annual Meeting of the American Association for Cancer Research;San Francisco, California, USA; April 06-10, 2002, March, 2002, pages 1078 - 1079, XP001179197, ISSN: 0197-016X *
KOBATA A ET AL: "Structure, pathology and function of the N-linked sugar chains of human chorionic gonadotropin", BIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR BASIS OF DISEASE, AMSTERDAM, NL, vol. 1455, no. 2-3, 8 October 1999 (1999-10-08), pages 315 - 326, XP004276915, ISSN: 0925-4439 *
KOBATA A: "Structural changes induced in the sugar chains of glycoproteins by malignant transformation of producing cells and their clinical application.", BIOCHIMIE. FRANCE NOV 1988, vol. 70, no. 11, November 1988 (1988-11-01), pages 1575 - 1585, XP002270856, ISSN: 0300-9084 *

Also Published As

Publication number Publication date
WO2003087821A2 (en) 2003-10-23
CA2481420A1 (en) 2003-10-23
EP1495323A2 (en) 2005-01-12
AU2003221671A1 (en) 2003-10-27

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