WO2003070908A2 - Novel spinosyn-producing polyketide synthases - Google Patents
Novel spinosyn-producing polyketide synthases Download PDFInfo
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- WO2003070908A2 WO2003070908A2 PCT/US2003/004998 US0304998W WO03070908A2 WO 2003070908 A2 WO2003070908 A2 WO 2003070908A2 US 0304998 W US0304998 W US 0304998W WO 03070908 A2 WO03070908 A2 WO 03070908A2
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- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
- C07D407/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
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- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
- C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
Definitions
- the present invention provides novel hybrid polyketide synthases (PKSs), DNA encoding such PKSs, vectors incorporating the hybrid polyketide synthase DNA, host organisms including but not limited to Saccharopolyspora spinosa strains transformed with the hybrid polyketide synthase DNA, methods of using the hybrid polyketide synthase DNA to change the products made by spinosyn-producing strains, and the novel biologically-active compounds generated by these manipulations.
- PKSs novel hybrid polyketide synthases
- fermentation product A83543 is a family of related compounds produced by Saccharopolyspora spinosa.
- the family of natural spinosyn compounds that have previously been isolated are described in US Pat. No. 6,274,350 Bl and WO 01/19840, along with their activities in a variety of insect control assays.
- a number of semi-synthetic spinosyn analogues are also described in U.S. Pat. No. 6,001,981, in which the chemically accessible areas of the spinosyn molecule were successfully substituted in a variety of ways.
- the spinosyn compounds are useful for the control of arachnids, nematodes and insects, in particular Lepidoptera and Diptera species, and they are quite environmentally friendly and have an appealing toxicological profile.
- the commercial product Spinosad is a mixture of spinosyns A and D (Pesticide Manual 11th ed., p. 1272).
- Tables 1 and 2 identify the structures of some known spinosyn compounds:
- the naturally produced spinosyn compounds consist of a 5,6,5-tricylic ring system, fused to a 12-membered macrocyclic lactone, a neutral sugar (rhamnose) and an amino sugar (forosamine) (see Kirst et al. (1991). If the amino sugar is not present the compounds have been referred to as the pseudoaglycone of A, D, etc., and if the neutral sugar is not present then the compounds have been referred to as the reverse pseudoaglycone of A, D, etc.
- a more preferred nomenclature is to refer to the pseudoaglycones as spinosyn A 17-Psa, spinosyn D 17-Psa, etc., and to the reverse pseudoaglycones as spinosyn A 9-Psa, spinosyn D 9-Psa, etc.
- the naturally produced spinosyn compounds may be produced via fermentation from cultures NRRL 18395, 18537, 18538, 18539, 18719, 18720, 18743 and 18823. These cultures have been deposited and made part of the stock culture collection of the Midwest Area Northern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, IL 61604.
- U. S. Pat. No. 5,362,634 and corresponding European Patent Application No. 375316 Al disclose spinosyns A, B, C, D, E, F, G, H, and J. These compounds are disclosed as being produced by culturing a strain of the novel microorganism Saccharopolyspora spinosa selected from NRRL 18395, NRL 18537, NRRL 18538, and NRRL 18539.
- WO 93/09126 disclosed spinosyns L, M, N, Q, R, S, and T. Also disclosed therein are two spinosyn J producing strains: NRRL 18719 and NRRL 18720, and a strain that produces spinosyns Q, R, S, and T: NRRL 18823.
- WO 94/20518 and US Pat. No. 5,670,486 disclose spinosyns K, O, P, U, V, W, and Y, and derivatives thereof. Also disclosed is spinosyn K-producing strain NRRL 18743.
- WO 01/19840 discloses spinosyn analogs produced by culturing Saccharopolyspora species LW107129 (NRRL 30141).
- WO 99/46387 and US Pat. No. 6,143,526 disclose the spinosyn biosynthetic genes from Saccharopolyspora spinosa.
- this process is similar to the biosynthetic pathway by which other macrolides (such as the antibiotic erythromycin, the antihelmintic avermectin, and the immunosuppressant rapamycin) are produced.
- the polyketide nucleus is assembled by a very large, multifunctional protein that is a Type I polyketide synthase (spn PKS).
- This polypeptide complex comprises a loading module and ten extension modules, each module being responsible for both the addition of a specific acyl-CoA precursor to the growing polyketide chain, and for the degree of reduction of the ⁇ -keto carbonyl group. Each module performs several biochemical reactions which are carried out by specific domains of the polypeptide.
- All the extension modules contain an acyl transferase (AT) domain that donates the acyl group from a precursor to an acyl carrier protein (ACP) domain, and a ⁇ -ketosynthase KS) domain that adds the pre-existing polyketide chain to the new acyl- ACP by decarboxylative condensation. Additional domains are present in some extension modules: ⁇ -ketoreductase (KR) domains reduce ⁇ -keto groups to hydroxyls, dehydratase (DH) domains remove hydroxyls to leave double bonds, and the enoyl reductase (ER) domain reduces a double bond to leave a saturated carbon.
- KR ⁇ -ketoreductase
- KR ⁇ -ketoreductase
- DH dehydratase
- ER enoyl reductase
- the loading module of the spn PKS is different from the extension modules in that it contains a variant KS domain (KSq), as well as AT and ACP domains.
- KSq domain which is also found in some other Type I PKS loading modules (but not all), is believed to provide the requisite starter unit by decarboxylation of an ACP- bound acyl chain (Bisang et al., 1999).
- the terminal extension module contains a thioesterase/cyclase (TE) domain that liberates the polyketide chain from the PKS.
- the spinosyn PKS DNA region consists of 5 ORFs with in-frame stop codons at the end of some ACP domains, similar to the PKS ORFs in the other macrolide-producing bacteria.
- the five spinosyn PKS genes are arranged head-to-tail, without any intervening non-PKS functions such as the insertion element found between the erythromycin PKS genes Al and All (Donadio et al, 1993). They are designated spnA, spnB, spnC, spnD, and spnE.
- the nucleotide sequence for each of the five spinosyn PKS genes, and the corresponding polypeptides are identified in US Pat. No. 6,143,526 and in Waldron et al., 2001. Also identified in these sources are the predicted translation products of the PKS genes, and the boundaries of the domains and modules.
- the spinosyn polyketide precursor condenses to form a macrocyclic lactone, referred to hereinafter as the polyketide nucleus.
- Production of insecticidally-active spinosyns requires additional processing of the polyketide nucleus.
- carbon-carbon bridges must be formed between C3 and C14, C4 and C12, and C7 and Cll, to generate the aglycone intermediate. Possible mechanisms for these unusual reactions have been suggested (Waldron et al., 2001), but the structural features of the polyketide substrate that are required for them to occur are not known.
- Second, a tri-O-methyl rhamnose must be incorporated at C9 to generate the pseudoaglycone.
- the enzymes involved in this step also have stringent substrate requirements: the forosaminyl transferase will not use the aglycone as a substrate, and the N-methyltransferase will not act on the forosamine after it has been attached to the pseudoaglycone.
- This substrate-specificity of later biosynthetic enzymes may be a barrier to producing novel, biologically-active spinosyns from precursors with different chemical structures.
- PKS polyketide synthase
- WO 93/13663 describes additional types of genetic manipulation of the ery PKS genes that are capable of producing altered polyketides.
- WO 98/01546 discloses replacement of the loading module of the ery PKS with the loading module from the avermectin (ave) PKS, to produce a hybrid Type I PKS gene that incorporates different starter units to make novel erythromycin analogues.
- the invention provides a hybrid spinosyn polyketide synthase that is capable of functioning in Saccharopolyspora spinosa to produce a biologically active spinosyn, said hybrid polyketide synthase comprising a heterologous loading module operatively associated with a plurality of Saccharopolyspora spinosa extender modules.
- the spinosyn loading domain is replaced with the loading domain for the erythromycin PKS or avermectin PKS.
- the ave and ery loading domains are of particular interest because they accept a variety of starter units.
- hybrid PKS genes in which the heterologous loading module incorporates an unusual starter unit, such as the loading module for rapamycin (cyclohexene carboxylic acid) or for myxathiazole (3-methyl butyric acid).
- an unusual starter unit such as the loading module for rapamycin (cyclohexene carboxylic acid) or for myxathiazole (3-methyl butyric acid).
- the required precursors e.g. cyclohexene carboxylic acid or 3-methyl butyric acid, may be provided in the culture medium, or the genes encoding their biosynthetic enzymes may be engineered into the organism so they are synthesized endogenously.
- the invention provides a hybrid spinosyn polyketide synthase that is capable of functioning in Saccharopolyspora spinosa to produce a biologically active 6-ethyl spinosyn compound, 16-ethyl spinosyn compound, 18-ethyl spinosyn compound, or 20-ethyl spinosyn compound, said hybrid polyketide synthase being the product produced by spinosyn biosynthetic DNA that has been modified so that the DNA for the AT domain of module 8, 3, 2, or 1, respectively, in the spinosyn PKS is replaced with DNA for an AT domain that normally incorporates ethyl malonyl-CoA.
- the DNA that encodes the relevant spinosyn AT domain is replaced with the DNA that encodes the AT domain of module 5 of the tylosin PKS or module 5 of the monensin PKS.
- the Streptomyces cinnamonensis crotonyl-CoA reductase is co-expressed.
- the invention provides a hybrid spinosyn polyketide synthase that is capable of functioning in Saccharopolyspora spinosa to produce a biologically active 18-methyl spinosyn compound, or 20-methyl spinosyn compound, said hybrid polyketide synthase being the product produced by spinosyn biosynthetic DNA that has been modified so that the DNA for the AT domain of module 2 or 1, respectively, in the spinosyn PKS is replaced with DNA for an AT domain that normally incorporates methyl malonyl-CoA.
- the invention provides a hybrid spinosyn polyketide synthase that is capable of functioning in Saccharopolyspora spinosa to produce a biologically active 16-desmethyl spinosyn compound, said hybrid polyketide synthase being the product produced by spinosyn biosynthetic DNA that has been modified so that the DNA for the AT domain of module 3 in the spinosyn PKS is replaced with DNA for an AT domain that normally incorporates malonyl-CoA.
- the AT domain of module 3 is replaced with the DNA that encodes the AT domain of module 2 of the rapamycin PKS
- the invention provides a process for producing a 6-ethyl spinosyn compound, a 21-desethyl-21-n-propyl spinosyn compound, or a 6-ethyl-21- desethyl-21-propyl spinosyn compound which comprises culturing a transgenic host organism that coexpresses crotonyl-CoA reductase with the spinosyn biosynthetic pathway.
- the host organism is transformed with DNA encoding the S. cinnamonensis crotonyl-CoA reductase.
- the invention provides a Saccharopolyspora spinosa strain that has been transformed with DNA encoding the S. cinnamonensis crotonyl-CoA reductase.
- the invention provides DNA encoding a hybrid spinosyn polyketide synthase of the invention, as described above.
- the invention provides a vector comprising DNA as described above.
- the invention provides a host organism comprising DNA as described above.
- the invention provides a compound of the formula (I)
- Rl is hydrogen, methyl, or ethyl
- R2 is hydrogen, methyl, or ethyl
- R3 is hydrogen
- R4 is methyl or ethyl, either of which may be substituted with one or more groups selected from halo, hydroxy, - alkyl, -C 4 alkoxy, C r C 4 alkylthio, or cyano;
- R4 is an alpha-branched C 3 -C 5 alkyl group, C 3 -C 8 cycloalkyl group, or C 3 -C 8 cycloalkenyl group, any of which may be substituted with one or more groups selected from halo, hydroxy, -C 4 alkyl, - alkoxy, C C 4 alkylthio, or cyano; or
- R4 is a 3-6 membered heterocyclic group that contains O or S, that is saturated or fully or partially unsaturated, and that may be substituted with one or more groups selected from halo, hydroxy, -C 4 alkyl, C r C 4 alkoxy, - alkylthio, or cyano;
- R5 is hydrogen or methyl
- R6 is hydrogen or methyl
- R7 is hydrogen or methyl
- R8 is hydrogen, methyl, or ethyl
- R9 is hydrogen, methyl, or ethyl; or a 5,6-dihydro derivative of a compound of formula I, provided that: a) R4 is methyl or ethyl substituted with one or more groups selected from halo, hydroxy, C r C 4 alkyl, C r C 4 alkoxy, - alkylthio, or cyano; or b) R4 is an alpha-branched C 3 -C 5 alkyl group, C 3 -C 8 cycloalkyl group, or C 3 -C 8 cycloalkenyl group, any of which may be substituted with one or more groups selected from halo, hydroxy, C r C 4 alkyl, C r C 4 alkoxy, C r C 4 alkylthio, or cyano; or c) R4 is a 3-6 membered heterocyclic group that contains O or S, that is saturated or fully or partially unsaturated, and that may be substituted with one or more groups selected
- Rl, R2, R3, R4, R5, R6, R7, R8, and R9 are as defined for Formula I.
- the invention provides a biologically pure culture of Saccharopolyspora spinosa selected from
- the invention provides a method of controlling pests which comprises delivering to a pest an effective amount of a compound of claim 1.
- the invention provides a pesticide composition comprising an effective amount of a compound of claim 1 as active ingredient in combination with an appropriate diluent or carrier.
- Figure 1 shows the construction of pCJR ⁇ l.
- Figure 2 shows the construction of vectors pLSB2 and pLSB3 used for expression of genes in S. spinosa.
- Figure 3 shows the construction of pLSB62, a vector to introduce the ery load into the spinosyn pathway.
- Figure 4 shows the hybrid ery/spn PKS pathway of S. spinosa 13E.
- Figure 5 shows the construction of pLSB29, a vector to introduce the ave load into the spinosyn pathway.
- Figure 6 shows the hybrid ave/spn PKS pathway of S. spinosa 21K2
- Figures 7a and 7b show the construction of pALK26, intermediate plasmid used to generate module 3 AT swap plasmids.
- Figure 8 shows the construction of pALK39, a vector used to introduce the rapAT2 into spinosyn module 3.
- Figure 9 shows the construction of pALK21, a vector designed to express the S. cinnamonensis crotonyl-CoA reductase gene in S. spinosa.
- Figure 10 shows the construction of pALK36, a vector used to introduce the tylAT5 into spinosyn module 3.
- Figure 11 shows the hybrid PKS pathway of strain S. spinosa 7D23.
- Figure 12 shows the hybrid PKS pathway of strain S. spinosa 36P4.
- SEQ ID NO:l is oligo PRISl.
- SEQ LD NO:2 is oligo PRIS2.
- SEQ ID NO: 3 is the DNA sequence of the promoter for resistance to pristinamycin.
- SEQ ID NO:4 is oligo CR311.
- SEQ ID NO:5 is oligo CR312.
- SEQ LD NO:6 is oligo SP28.
- SEQ LD NO:7 is oligo SP29.
- SEQ ID NO 8 is the fragment of DNA encoding the ery PKS loading module which was used for cloning, with introduced restriction enzyme sites at bp 1-6 and bp 1680-1685.
- SEQ ID NO:9 is oligo SP14.
- SEQ ID NO:10 is oligo SP15.
- SEQ LD NO: 11 is the fragment of DNA encoding the ave PKS loading module which was used for cloning, with introduced restriction enzyme sites at bp 1-6 and bp 1689-1694.
- SEQ LD NO: 12 is oligo CR322.
- SEQ LD NO: 13 is oligo CR323.
- SEQ ID NO: 14 is oligo CR324.
- SEQ ID NO: 15 is oligo CR325.
- SEQ ID NO: 16 is oligo CR328.
- SEQ ID NO: 17 is oligo CR329.
- SEQ LD NO: 18 is oligo CR330.
- SEQ LD NO:19 is oligo CR321.
- SEQ LD NO:20 is the fragment of DNA encoding the rap AT2 which was used for cloning, with introduced restriction enzyme sites at bp 1-6 and bp 832-837.
- SEQ LD NO:21 is oligo CCRMONF.
- SEQ LD NO:22 is oligo CCRMONR.
- SEQ ID NO:23 is the DNA sequence used for expression of the Streptomyces cinnamonensis crotonyl-CoA reductase, with introduced restriction sites at bp 1-6 and 1389-1394.
- SEQ ID NO 24 is oligo AK1.
- SEQ ID NO 25 is oligo AK2.
- SEQ ID NO 26 is the fragment of DNA encoding the tyl AT5 which was used for cloning, with introduced restriction enzyme sites at bp 1-6 and bp 967-972.
- Saccharopolyspora spinosa NRRL 18537 or Saccharopolyspora spinosa NRRL 18538 and producing the compounds of the invention had the following culture characteristics:
- S. erythraea (Blanc et al. 1995; Sala-Bey et al. 1995). It is a promoter that can be induced by physiological stresses in Streptomyces spp.
- heterologous promoters in S. spinosa is not limited to those described above, and could include others which might be expected to function in S. spinosa.
- the invention provides hybrid PKSs that are functional in Saccharopolyspora spinosa to generate polyketides that are processed to biologically-active spinosyns.
- the resulting polyketides were extended to the same length as the natural spinosyns, and processed by cross-bridging and glycosylation to generate novel insecticidal spinosyns.
- the hybrid PKS comprises the extension modules from the spn PKS with a heterologous loading module that leads to a spinosyn polyketide having a different starter unit.
- Hybrid spinosyn PKS genes that contain the spn extender modules behind a heterologous loading module can provide novel spinosyns with different side chains at C21.
- this side chain is determined by the starter unit which the loading module selects to initiate polyketide synthesis. Changes in the side chain may alter the physical properties or biological activity of the resulting spinosyn. It is particularly useful to provide a hybrid PKS gene in which the loading module accepts many different carboxylic acids, including unnatural acids. Such a gene can be used to generate many different spinosyns by incorporation of different starter units.
- the loading module of the spn PKS can be replaced by the loading module of the ave PKS, which is known to accept a wide variety of starter units (Dutton et al, 1991).
- the loading module of the ery PKS is also known to accept alternate starter units (Pacey et al., 1998).
- an organism expressing such hybrid spinosyn PKS genes can produce novel spinosyns in which the nature of the side chain at C21 is determined by the carboxylic acid that is fed to the organism.
- the side chains can be of different lengths, with branches or cycles, and/or contain heteroatoms.
- the hybrid PKS includes a loading module that accepts many different carboxylic acids so the hybrid gene assembly can be used to produce many different spinosyns.
- Particularly useful examples contain the spn extension modules with the loading module from the erythromycin (ery) PKS, or the loading module from the avermectin (ave) PKS.
- ery erythromycin
- a. Hybrid spinosyn PKS using ery loading module
- the loading module of the erythromycin biosynthetic cluster (ervATOACPO) governs the introduction of propionyl-CoA into the starter of the erythromycin molecule. It has been shown previously that alternative starters can be incorporated into the erythromycin molecule by feeding free acids to the production medium (Pacey et al. 1998).
- the erythromycin loading module was spliced to the beginning of the spinosyn KS1 just within the KS domain in the conserved region. New domain or module connections are preferably made at conserved DNA sequences within domains, or close to the edges thereof; however, an active polyketide synthase can alternatively be generated by engineering splice sites in the interdomain regions (WO 98/01546).
- the ery load fragment was cloned in-frame with, and upstream of, a region of spnA to allow homologous recombination with the native spn PKS.
- Upstream of the ery load was either the actl promoter (P ⁇ C tZ) or me promoter for resistance to pristinamycin (Pptr), giving rise to the plasmids pLSB61 and pLSB62 respectively.
- These plasmids are based on pKCl 132 and are therefore apramycin-resistant. They also carry oriT for conjugal transfer of DNA into actinomycetes (Bierman et al. 1992, Matsushima et al. 1994). These constructs were transformed into S. spinosa NRRL 18537 by conjugation. Exconjugants were confirmed to contain the hybrid ery/spn PKS under the appropriate promoter by PCR amplification.
- S. spinosa NRRL 18537:pLSB62 was designated S. spinosa 13E, and was used for analysis of spinosyn production.
- a number of carboxylic acids were fed to S. spinosa 13E, leading to the production of novel spinosyn analogues with altered starter units.
- novel spinosyns identified were 2 l-desethyl-21 -cyclopropyl spinosyns A and D (by incorporation of cyclopropane carboxylic acid), 2 l-desethyl-21 -cyclobutyl spinosyns A and D (by incorporation of cyclobutane carboxylic acid) and 21-desethyl-21-methylthiomethyl spinosyns A and D (from methylthioacetic acid).
- novel analogues showed reasonable chromatographic retention times, characteristic UN chromophores and MS fragmentation patterns, and the predicted structures were supported by accurate mass measurements. Structural assignments of the isolated 21 -cyclopropyl and 21-cyclobutyl compounds were confirmed by full ⁇ MR characterization. Compounds isolated were active in insect control assays.
- strain S. spinosa 13E is not limited to the production of these compounds. It is expected that a number of other spinosyn analogues can be identified by feeding other acids, such as those used to produce novel erythromycins (Pacey et al. 1998).
- Plasmid pCJR81 is a vector for expression of polyketide genes under the promoter for resistance to pristinamycin. It was constructed as follows:
- oligos Two overlapping oligos were designed to perform a PCR reaction in which they act both as primers and template. They were designed to introduce an Ndel restriction site incorporating the ATG start codon, such that genes can be cloned with optimal spacing from the ribosome binding site. A Spel restriction site was incorporated to facilitate further cloning.
- the oligos are PRIS1 (SEQ LD ⁇ O:l) and PRIS2(SEQ LD NO:2).
- Amplification to obtain the promoter fragment was performed with Pwo thermostable DNA polymerase using the manufacturer's conditions.
- the 112 bp fragment was phosphorylated with T4 polynucleotide kinase, and cloned into commercially-available pUC18 digested with Smal and dephosphorylated. Plasmids containing inserts were sequenced. One plasmid containing the correct sequence was designated pRIS4.
- Plasmids pLSB2 and pLSB3 were constructed for expression of polyketide genes or accessory genes in S. spinosa. Plasmid pLSB2 contains the actl promoter (P a ct ⁇ ) ⁇ d lts cognate activator, actII-ORF4. Plasmid pLSB3 contains the promoter for resistance to pristinamycin (Pptr)- These plasmids were constructed as follows:
- Plasmid pKCl 132 (Bierman et al. 1992) contains an origin of transfer (oriT), and an apramycin resistance marker for selection in both E. coli and actinomycetes. It can therefore be used for DNA manipulations in E. coli, and permit the final plasmids to be introduced into S. spinosa by conjugation.
- the polylinker of pKCl 132 was replaced by a linker of two oligos CR311 (SEQ LD NO:4) and CR312 (SEQ ID NO:5) in order to provide appropriate EcoRY/Spe ⁇ /Ndel/Xbal restriction sites.
- Plasmid pKCl 132 was digested with PvwII and the ends dephosphorylated with shrimp alkaline phosphatase.
- the oligos CR311 and CR312 were phosphorylated with T4 polynucleotide kinase, annealed and cloned into pKCl 132_PvuII to generate pLSB 1.
- a Spel/Ndel fragment containing the actinorhodin pathway specific activator, actII-ORF4 and the ⁇ ctl promoter was isolated from pCJR24 (WO 98/01546, WO 98/01571, Rowe et al.
- Example 3 Construction of a vector to incorporate the loading module of the erythromycin polyketide synthase into the spinosyn polyketide synthase
- the vector to incorporate the loading module of the erythromycin polyketide synthase into the spinosyn polyketide synthase contains the erythromycin loading module (ATOACPO), followed by a region of the first module of the spinosyn PKS to provide homology for integration.
- the vector is designated pLSB62 and was constructed as follows.
- the erythromycin loading module was amplified by PCR using pCJR26 (Rowe et al. 1998) as the template, and oligos SP28 (SEQ LD NO:6) and SP29 (SEQ LD NO:7).
- SP28 incorporates anN el site at the start codon of the ery sequence
- SP29 incorporates an Nhel site at the beginning of the KS1 domain.
- the PCR fragment was phosphorylated, gel-purified and cloned into pUC18 which had been previously digested with Smal and dephosphorylated. Clones were screened for the presence of inserts and sequenced. One clone containing the correct sequence was designated pLSB44. It contained the insert in the orientation with the Nhel site close to the EcoRI site of the polylinker. The sequence of the erythromycin loading module fragment used, from the Ndel site to the Nhel site is shown in SEQ LD NO 8.
- Plasmid pLSB8 (described in Example 11) contains a fragment of spnA starting with the Nhel site at sp ⁇ KS 1.
- the fragment containing the erythromycin loading module was excised from pLSB44 as anNdel/Nhel fragment and cloned into pLSB8 previously digested with Ndel and Nhel, to give pLSB56.
- the fragment contained in pLSB56 contains the erythromycin loading module spliced in-frame to the spinosyn KS1, with a region of homology to spnA which is sufficient for integration to occur.
- This region was removed as an NdellXbal fragment and cloned into pLSB3 to give pLSB62. This places the new ery/spn hybrid fragment under in a vector which can be transferred into S. spinosa by conjugation and selected using the apramycin resistance marker.
- Example 4 Generation of a S. spinosa strain harbouring a hybrid polyketide synthase comprising the erythromycin loading module fused to the KS1 of the spinosyn PKS.
- Saccharopolyspora spinosa NRRL 18537 was transformed by conjugation (Matsushima et al. 1994) fromE. coli S17-1 (Simon et al., 1983) with ⁇ LSB62. Transformants were selected for apramycin resistance and screened by PCR. A single transformant was designated strain S. spinosa 13E.
- HPLC method is useful for analyzing fermentations for the production of natural spinosyns and novel non-natural engineered spinosyns.
- Mobile phase A 10% acetonitrile:90% water, containing lOmM ammonium acetate and 0.15% formic acid.
- Mobile phase B 90% acetonitrile:10% water, containing lOmM ammonium acetate and 0.15% formic acid.
- S. spinosa 13E was cultured from a frozen vegetative stock (1:1 CSM culture ryopreservative, where the cryopreservative is 10% lactose, 20% glycerol w/v in water).
- a primary pre-culture was grown in CSM medium (tryptic soy broth 30 g/1, yeast extract 3 g/1, magnesium sulfate 2 g/1, glucose 5 g/1, maltose 4 g/1; Hosted and Baltz 1996; US Pat. No.
- the secondary vegetative pre-culture was used to inoculate production medium (glucose 67 g/1, Proflo cottonseed flour 25 g/1, peptonized milk nutrient 22 g/1, corn steep liquor 12 g/1, methyl oleate 40 g/1, calcium carbonate 5 g/1, pH to 7.0 with sodium hydroxide; Strobel and Nakatsukasa 1993) at 5% v/v.
- Small scale production cultures were fermented under the same conditions as the pre-cultures, but for 7-10 days. For initial S. spinosa 13E production, small-scale cultures were grown in 30 mL of production medium in 250 mL Erlenmeyer flasks with springs for 7 days.
- Example 7 Precursor-directed production of novel spinosyns from S. spinosa 13E (Production of Compounds 1-6).
- the ery/spn hybrid PKS was used to generate novel spinosyn metabolites by feeding carboxylic acids to production cultures.
- the ery loading module incorporated the carboxylic acid within the starter of the molecule.
- Frozen vegetative stocks of S. spinosa 13E were inoculated into primary vegetative pre-cultures in CSM (50 mL incubated in a 250 mL Erlenmeyer flask with spring). Secondary pre-cultures in vegetative medium (250 mL incubated in a 2 L Erlenmeyer flask with spring) were prepared and incubated as described in Example 6, but at 300 rpm with a one-inch throw.
- Production medium was inoculated with the secondary vegetative pre-culture at 5% v/v, and allowed to ferment in a 20 L stirred bioreactor for 7-10 days at 30°C. Airflow was set at 0.75 wm, over pressure was set at 0.5 barg or below, and impeller tip speed was controlled between 0.39 and 1.57 ms "1 in order to maintain dissolved oxygen tension at or above 30% of air saturation. Additional Pluronic L0101 (BASF) was added to control foaming, if needed.
- BASF Pluronic L0101
- Cyclobutyl carboxylic acid was fed to the bioreactor at 25 hours, to a final concentration of 5 mM.
- the fermentation broth was harvested after 7 days and clarified by centrifugation to provide supernatant and cells.
- the cells (1 L) were extracted by mixing thoroughly with an equal volume of methanol then allowed to stand for 30 min.
- the cell-methanol slurry was centrifuged, and the supernatant decanted off. The procedure was repeated.
- the fermentation supernatant (12 L) was adjusted to pH ⁇ 10 by addition of 5 N NaOH and stirred gently with 0.75 volumes of ethyl acetate for 8 hours.
- the upper phase was removed by aspiration and the extraction repeated.
- the ethyl acetate and methanol fractions were combined and the solvents removed in vacuo to yield a yellow-brown oil/aqueous mixture (1 L). This was mixed with ethyl acetate (2 L) and extracted with a solution of 50mM tartaric acid (3 x 1.5 L). The tartaric acid extracts were combined, adjusted to pH ⁇ 10 with 5 N NaOH, and extracted with ethyl acetate (3 x 1.5 L). The ethyl acetate extracts were combined and the solvent removed in vacuo to leave a brown oily residue (7.5 g). The residue was dissolved into ethyl acetate (500 mL) and exfracted three times with 50 mM tartaric acid (350 mL).
- the tartaric acid fractions were combined, adjusted to pH ⁇ 10 and re-extracted with ethyl acetate (3 x 500 mL). The ethyl acetate fractions were combined and the solvent removed in vacuo to yield a brown oily residue (0.5 g).
- Mobile-phase A 10% acetonitrile/90% water, containing 10 mM ammonium acetate and 0.15% formic acid.
- Mobile-phase B 90% acetonitrile/10% water, containing 10 mM ammonium acetate and 0.15% formic acid.
- Fractions were collected every 30 seconds. Fractions from the initial fractionation that contained 2 l-desethyl-21 -cyclobutyl spinosyn A were combined, and the solvent removed in vacuo. The residues were chromatographed over reversed-phase silica gel (Prodigy C 18 , 5 ⁇ m; 10 x 250 mm) eluting with a gradient as described below, at a flow rate of 5 mL/min.
- Fractions were collected every 30 seconds. Fractions containing the 2 l-desethyl-21 - cyclobutyl spinosyn A were combined, the acetonitrile removed in vacuo, and the sample concentrated using C j g-BondElute cartridges (200 mg). The sample was applied under gravity, washed with water (10 mL) and eluted with methanol (2 x 10 mL), then the solvent removed in vacuo. Fractions from the initial crude fractionation that contained 2 l-desethyl- 21 -cyclobutyl spinosyn D were combined and the solvent removed in vacuo. The residues were chromatographed over reversed-phase silica gel (Prodigy C 18 , 5 ⁇ m; 10 x 250 mm) eluting with a gradient as described below, at a flow rate of 5 mL/min.
- Fractions were collected every 30 seconds. Fractions containing the 2 l-desethyl-21 - cyclobutyl spinosyn D were combined, the acetonitrile removed in vacuo, and the sample concentrated using C 18 -BondElute cartridges (200 mg capacity). The sample was applied under gravity, washed with water (10 mL) and eluted with methanol (2 x 10 mL), and the solvent removed in vacuo.
- the chemical structures of the new spinosyns were determined by spectroscopic methods, including nuclear magnetic resonance spectroscopy (NMR), mass specuOmetry (MS), ultraviolet spectrometry (UN), coupled high performance liquid chromatography-mass specfrometry (HPLC-MS), and by comparison to the spectral data for the known compounds spinosyns A, D, E and F.
- NMR nuclear magnetic resonance spectroscopy
- MS mass specuOmetry
- UN ultraviolet spectrometry
- HPLC-MS coupled high performance liquid chromatography-mass specfrometry
- Table 6 shows the ! H and 13 C ⁇ MR chemical shift data for 2 l-desethyl-21 -cyclobutyl spinosyn A in CDC1 3 .
- Frozen vegetative stocks of S. spinosa 13E were inoculated into primary vegetative pre-cultures in CSM (50 mL incubated in a 250 mL Erlenmeyer flask with spring). Secondary pre-cultures in vegetative medium (250 mL incubated in a 2 L Erlenmeyer flask with spring) were prepared and incubated as described in Example 8.
- Production medium was inoculated with the secondary pre-culture at 5% v/v and allowed to ferment in a 20 L stirred bioreactor for 7-10 days under the conditions described in Example 8. Cyclopropyl carboxylic acid was fed to the bioreactor at 45 hours to a final concentration of 5 mM. The fermentation broth was harvested after 7 days and extracted as described in Example 8.
- BASF Pluronic L-0101
- Table 7 summarizes the *H and 13 C ⁇ MR spectral data for 2 l-desethyl-21 -cyclopropyl spinosyn A in CDC1 3 .
- the loading module of the avermectin biosynthetic cluster governs the introduction of C-2 branched starter units into the avermectin molecule, derived from iso- butyryl-CoA and 2-methylbutyryl-CoA.
- C-2 branched starter units into the avermectin molecule, derived from iso- butyryl-CoA and 2-methylbutyryl-CoA.
- the avermectin loading module has also been shown to incorporate CoA-esters of a broad range of free acids from the production medium, either in its native environment, or as part of the ave/ ery hybrid pathway (Pacey et al. 1998).
- the avermectin loading module swap described in the literature is actually a replacement of the erythromycin ATOACPO by the avermectin ATOACPO. This leads to a piece of erythromycin D ⁇ A sequence upstream of the avermectin AT0 between the start codon and a Spel site.
- the resulting hybrid gene is an ery/ave/spn hybrid, but since it transfers the specificity of the avermectin loading module to the spinosyn PKS, it has been designated an ave/spn hybrid through-out.
- ATOACPO avermectin loading module
- pLSB29 (with the hybrid PKS region under the control of P ⁇ c t/) and pLSB30 (with the hybrid PKS region under the control of *ptr) are based on pKCl 132 and therefore contain the apramycin resistance marker for selection both in E. coli and S. spinosa, and the oriT for conjugal transfer of DNA from E. coli to S. spinosa (Bierman et al. 1992, Matsushima et al. 1994). These constructs were transformed into S. spinosa NRRL 18538 by conjugation. Exconjugants were confirmed to contain the hybrid ave/spn PKS gene under the appropriate promoter by PCR analysis.
- S. spinosa NRRL 18538:pLSB29 was designated S. spinosa 21K2. It produced mainly spinosyns E, A and D by incorporation of acetate and propionate into the loading module. Additional small peaks were observed in LC-MS, with masses that were consistent with the novel natural products 21-desethyl-21-iso-propyl spinosyn A and 2 l-desethyl-21 -sec-butyl spinosyn A and the equivalent D analogues. These minor products resulted from incorporation of iso-butyrate and 2-methyl butyrate respectively into the starter.
- the ave loading module recruits only these branched starters.
- a broader spectrum of products was observed when the ave loading module was spliced upstream of the KS1 in the erythromycin PKS, indicating that such a hybrid can incorporate acetate and propionate as well.
- Free acids which can be used in this way include, but are not limited to, the following: Cyclic organic acids including cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl and 2-methylcyclopropyl; heteroatom containing cyclic organic acids including 2-furoic acid, 3-furoic acid, thiophene carboxylic acid and methyl thiophene carboxylic acid; branched chain carboxylic acids; and some other acids which included methylthioacetic acid, chloroacetic acid, cyanoacetic acid and methoxyacetic acid.
- Preferred candidates for the C21 substituent are the groups of carboxylate units useable as substrates by the ery or ave loading modules.
- Preferred substrates include cyclobutane carboxylic acid and cyclopropane carboxylic acid. Other possibilities include n-butyric acid, iso-propyl carboxylic acid, 2-(S)-methylbutyric acid, 2-methylcyclopropane carboxylic acid, 3-furoic acid and methylthioacetic acid.
- Feeding the branched chain acids iso-propyl and 2-methyl butyl carboxylic acid did not alter the yield of novel natural products made due to the normal specificity of the ave load. These products were observed in all healthy cultures of S. spinosa 21K2 in low yield.
- Cyclobutyl carboxylic acid was accepted by the ave loading module to give the A and D analogues, and a substantial proportion of the C 17-pseudoaglycone of the A analogue.
- the cyclobutyl products were observed at higher levels than in the fed hybrid ery/spn system, with 2 l-desethyl-21 -cyclobutyl spinosyn A at -5-10 mg/litre, 2 l-desethyl-21 -cyclobutyl spinosyn D at lower but significant levels and 2 l-desethyl-21 -cyclobutyl spinosyn A 17- pseudoaglycone at -10-15 mg/litre.
- the cyclopropyl carboxylic acid feed was also successful, yielding 2 l-desethyl-21 -cyclopropyl spinosyns A and D.
- the two cyclobutyl analogues and the cyclopropyl A analogue were shown to be identical to the purified compounds isolated from the ery load experiment.
- Methylthioacetic acid was incorporated to give the 21-desethyl-21-methylthiomethyl spinosyn A, at yields comparable to those of the cyclobutyl experiment above. This was a significant improvement in yield over production of the same compound from the ery load experiment. 2-furoic acid and methylcyclopropyl carboxylic acid were also incorporated to give the expected novel products.
- Example 11 Construction of a vector to incorporate the loading module of the avermectin polyketide synthase into the spinosyn polyketide synthase
- the vector to incorporate the loading module of the avermectin polyketide synthase into the spinosyn polyketide synthase contains the avermectin loading module (ATOACPO) followed by a region of the first module of the spinosyn PKS to provide homology for integration.
- the vector is designated pLSB29 and was constructed as follows:
- the avermectin loading module has been previously spliced upstream of the erythromycin module 1 (WO 98/01546, WO 98/01571, Marsden et al. 1998).
- the loading module had the DNA coding for the ery amino acid sequence at the start, followed by the ATOACPO of the ave PKS.
- This hybrid fragment conferred the specificity of the avermectin loading domain, although it included a small amount of ery sequence. The same fragment was used in this experiment, and leads to an ery/ave/spn hybrid protein.
- the fragment begins with an Ndel site incorporating the start codon, and ends with a Nhel site engineered at the beginning of the KS1. This introduces a conservative amino acid change (He - Leu) in the spinosyn KS1 sequence.
- the region of spnA from the beginning of the KS1 was amplified by PCR using pRHB3El 1 (US 6,274,350 Bl, Waldron et al. 2001) as the template, and oligos SP14 (SEQ ID NO:9) and SP15 (SEQ LD NO:10).
- SP14 introduces an Nhel site at bases 24107-24112 (numbers refer to SEQ LD NO:l of US 6,274,350).
- SP15 binds approximately 1500 bp downstream, at a BstEII site (25646 -25652). A Notl site was also incorporated into SP15 for the subsequent cloning step.
- the PCR was carried out using Pwo thermostable polymerase under standard conditions.
- the PCR product was phosphorylated, gel-purified and cloned into pUC19 which had been previously digested with Smal and dephosphorylated. A number of insert-containing clones were sequenced. One clone containing the insert in the orientation which places the Nhel site next to the EcoRI site in the vector was designated pLSB5.
- the avermectin loading module was then cloned from pIGl (WO 98/01546, WO 98/01571, Marsden et al. 1998) as an Ndel/Nhel fragment and ligated into pLSB8 previously digested with Ndel and Nhel.
- the DNA sequence of the avermectin loading module fragment used, from the Ndel site to the Nhel site is shown in SEQ LD NO 11.
- the resulting plasmid was designated pLSB14.
- the fragment contained in pLSB14 contains the avermectin loading module spliced in-frame to the spinosyn KS1, with a region of homology to spnA which is sufficient for integration to occur.
- This fragment was then removed as an Ndel/Xbal fragment and cloned into pLSB2 to give pLSB29. This places the new ave/spn hybrid fragment under Pactl, in a vector which can be transferred into S. spinosa by conjugation and then selected using the apramycin resistance marker.
- Example 12 Generation of a S. spinosa strain harbouring a hybrid polyketide synthase comprising the avermectin loading module fused to the KS1 of the spinosyn PKS.
- Saccharopolyspora spinosa NRRL 18538 was transformed by conjugation (Matsushima et al. 1994) from E. coli S17-1 (Simon et al., 1983) withpLSB29. Transfonnants were selected by resistance to apramycin and screened by Southern blot analysis. A single transformant displaying the correct hybridization pattern to show that the plasmid had integrated into the chromosome by homologous recombination was designated strain S. spinosa 21K2.
- S. spinosa 21K2 was cultured from a frozen vegetative stock used to inoculate CSM medium (Hosted and Baltz 1996; US 5,362,634). This pre-culture was grown in flasks shaken at 300 rpm with a one-inch throw at a temperature of 30°C for 3 days. This was used to inoculate vegetative medium (Strobel and Nakatsukasa 1993; US Pat. No. 5,362,634) at 5% v/v and was cultured under the same conditions for a further 2 days. The vegetative culture was used to inoculate production medium (Strobel and Nakatsukasa 1993) at 5% v/v.
- Small-scale production cultures were fermented under the same conditions as the pre- cultures, but for 7-10 days at 250 rpm with a two-inch throw and at 75% relative humidity. Initial small-scale production cultures were grown in 6 mL of production medium in 25 mL Erlenmeyer flasks with springs for 7 days.
- Example 14 Precursor-directed production of novel spinosyns from S. spinosa 21K2 (Production of Compounds 1-6 and 13-20)
- the ave/spn hybrid PKS was used to generate novel spinosyn metabolites by feeding carboxylic acids to production cultures.
- the avermectin loading module incorporated the carboxylic acid within the starter of the molecule.
- Example 15 Isolation of novel metabolites from a large-scale fermentation of S. spinosa
- Frozen vegetative stocks of S. spinosa 21K2 were inoculated into primary vegetative pre-cultures of S. spinosa 21K2 in CSM (50 mL incubated in a 250 mL Erlenmeyer flask with spring). Secondary pre-cultures in vegetative medium (250 mL incubated in a 2 L Erlenmeyer flask with spring) were prepared and incubated as described in Example 6.
- Production medium was inoculated with the secondary pre-culture at 5% v/v and allowed to ferment in a 20 L stirred bioreactor for 7-10 days under the conditions described in Example 8.
- 2-Methyl butyric acid to a final concentration of 2 mM, was fed to the bioreactor at 26 and 37.5 hours, leading to an overall final concentration of 4mM.
- the fermentation broth was harvested after 7 days and extracted as described in Example 8.
- Fractions were collected every 30 seconds. Fractions containing the 2 l-desethyl-21 -isopropyl spinosyn A were combined, the acetonitrile removed in vacuo, and the sample concentrated using C 18 -BondElute cartridges (200 mg). The sample was applied under gravity, washed with water (10 mL) and eluted with methanol (2 x 10 mL), then the solvent was removed in vacuo.
- Table 11 summarizes the X H and 13 C ⁇ MR chemical shift data for 2 l-desethyl-21 -iso-propyl spinosyn A in CDC1 3 .
- the putative 2 l-desmethyl-21 -sec-butyl spinosyn A (Compound 11) has the following characteristics.
- the invention provides engineered S. spinosa hosts which present an altered substrate supply such that the native polyketide synthase produces novel products.
- S. cinnamonensis crotonyl-CoA reductase with the spinosyn biosynthetic pathway leads to novel products where the loading module additionally incorporates ethyl malonyl-CoA to yield 21-desethyl-21-n-propyl spinosyns A and D, and the AT of module 8 additionally incorporates ethyl malonyl-CoA to yield 6-ethyl spinosyn A.
- both of these AT domains can accept ethyl malonyl-CoA to yield 6-ethyl-21- desethyl-21-n-propyl spinosyn.
- the spinosyn loading module comprises a KSqATOACPO which predominantly decarboxylates methyl malonyl-CoA to incorporate propionate.
- Loading modules containing a KSq are generally more specific than those that lack them. It is therefore interesting and unusual that the spinosyn loading module occasionally accepts malonyl-CoA (naturally producing trace amounts of spinosyn E) and, as disclosed here, unexpectedly accepts ethyl malonyl-CoA when it is present.
- the invention provides hybrid spinosyn PKSs comprising a heterologous extension module or a spinosyn extension module with a heterologous AT domain.
- the AT domains select the extender units that are incorporated into the growing polyketide chain.
- the extender is predominantly malonyl-CoA.
- the AT in module 3 is essentially specific for methyl malonyl-CoA. It occasionally incorporates malonyl-CoA, leading to the accumulation of spinosyn F as a natural minor component.
- the AT in module 8 shows a relaxed specificity, incorporating predominantly malonyl-CoA (to give spinosyn A) but also incorporating a significant amount methyl malonyl-CoA (to give about 15% spinosyn D).
- the amino acid sequence of the module 8 AT is similar to other AT domains in regions that are associated with the selection of methyl malonyl-CoA. We suggest therefore that the incorporation of predominantly malonyl-CoA at this position is a reflection of substrate supply, in combination with an AT domain that has an unusually loose specificity.
- the AT of the loading module is largely selective for methyl malonyl-CoA, but incorporates malonyl-CoA 5-10% of the time (leading to production of the natural minor component spinosyn E).
- a system was constructed to allow AT swaps to be carried out in module 3 of the spinosyn polyketide synthase.
- the AT of module 3 naturally incorporates methyl malonyl- CoA and introduces the 16-methyl branch in spinosyn.
- One way to effect an AT swap is by replacement, which will result in a strain with the same transcripts as in the native PKS.
- An alternative method is via single integration, which places the complete plasmid sequence into the spinosyn PKS genes and requires that a promoter be infroduced to drive the genes downstream of the integration site.
- a spinosyn PKS was generated in which the module 3 AT (specific for methyl malonyl-CoA) was replaced by the rapamycin module 2 AT (malonyl-CoA specific).
- the resulting strain was designated S. spinosa 7D23.
- the PKS genes of 7D23 contain spnA, spnB and a truncated spnC under the native spnA promoter, followed by the plasmid sequence (including the apramycin resistance marker) and the hybrid spnC and spnD and spnE under control of an introduced promoter.
- the heterologous promoter used was the promoter for resistance to pristinamycin.
- Alternative promoters could also be used, for example the actl promoter with its cognate activator actII-ORF4.
- S. spinosa 7D23 produced the four predicted spinosyn analogues, spinosyn F, spinosyn F 17-pseudoaglycone, 16-desmethyl spinosyn D and 16-desmethyl spinosyn D 17- pseudoaglycone.
- the yields were unexpectedly high, being only 3-fold lower than from a non-engineered strain (see Table 12 within Example 20).
- the spinosyn F compounds have been identified previously, as minor components in non-engineered strains, but this manipulation of the PKS genes resulted in a dramatic increase in their yield.
- the abundance of the pseudoaglycone F may be a reflection of a reduced activity of the forosamine glycosyl transferase for this novel substrate.
- the final glycosylation occurs at C-17, so the neighboring C-16 methyl group may be important for substrate recognition.
- the spinosyn module 3 AT domain was amplified by PCR using pRHB3El 1 as the template and the primers CR322 (SEQ LD NO: 12) and CR323(SEQ ID NO: 13). These primers introduce Mscl and ⁇ vrll sites (bp 3-8 of SEQ ID NOS: 12 and 13) flanking the AT domain.
- the 973 bp PCR product was phosphorylated and cloned into commercially-available pUC18 which had previously been digested with Smal and dephosphorylated. Insert-containing transformants were screened for orientation and sequenced.
- flanking region downstream of the spinosyn module 3 AT was amplified by PCR using pRHB3El 1 as the template and the primers CR324 (SEQ ID NO: 14) and CR332 (SEQ ID NO: 15).
- Primer CR324 introduces an ⁇ vrll site (bp 3-8 of SEQ ID NO: 14) at exactly the same position as CR323, and CR332 binds downstream of a Pstl site which is naturally occurring in the sequence.
- the 1557 bp PCR product was phosphorylated and cloned into commercially-available pUC18 which had previously been digested with Smal and dephosphorylated. Insert-containing transformants were screened for orientation and sequenced.
- pALK9 was digested with AvrTl and Pstl.
- the 1525 bp fragment was gel-purified and cloned into pALK6 digested with Avrll and Pstl, to give plasmid pALKl 1.
- pALK12 was constructed to provide a suitable polylinker for this experiment.
- pALK6 was digested with Ndel and stl and ligated with an annealed linker of oligos CR328 (SEQ LD NO: 16) and CR329 (SEQ LD NO: 17).
- the linker was designed to destroy the Ndel site and leave Xbal, EcoRV and Pstl sites. Insert-containing clones were analysed by restriction enzyme digestion and a single clone displaying the correct pattern was designated pALK12.
- flanking region upstream of the spinosyn module 3 AT was amplified by PCR using pRHB3Ell as the template and the primers CR330 (SEQ LD NO:18)and CR321 (SEQ ID NO: 19).
- Primer CR330 introduces an M site (bp 18-23 of SEQ LD NO: 18) at the ATG start codon of the spnC gene, and CR321 incorporates an Mscl site (bp 3-8 of SEQ ID NO: 19) at exactly the same position as CR322.
- the 1729 bp PCR product was phosphorylated and cloned into commercially-available pUC18 which had previously been digested with Smal and dephosphorylated.
- Insert-containing transformants were screened for orientation and sequenced.
- pALK12 was digested with Pstl ⁇ axdXmnl and the 601 bp cloned into PstllXmnl digested pALK23 to give pALK24.
- pALKl 1 was digested with Pstl and Mscl and the 2488 bp fragment was ligated into the 3777 bp backbone produced by digesting pALK24 with Pstl and Mscl, to give pALK25.
- pALK26 is the intermediate plasmid in the experiments to generate spinosyn PKS genes with AT swaps in module 3. It contained the spinosyn AT3 with flanking restriction sites, the upstream region o ⁇ spnC to the start codon, and a region downstream for homologous recombination. It was missing a 407 bp Mscl-Mscl fragment just upstream of the Mscl site at the edge of the AT, which has to be inserted after the AT swap has been achieved.
- Example 17 Construction of a vector which can be used to engineer the spinosyn biosynthetic pathway to produce C-16 desmethyl spinosyns
- Plasmid pALK39 was used to integrate into the chromosome of S. spinosa by homologous recombination. It was designed to incorporate the rapamycin AT2 into spinosyn module 3. Such an engineered S. spinosa strain produced C-16 desmethyl spinosyns and intermediates in this pathway. Plasmid pALK39 was constructed as described below.
- the rapamycin module 2 was excised from pCJR26 as anMscI ⁇ 4vr ⁇ fragment (see SEQ LD NO 20) and ligated into pALK26 which had previously been digested with Mscl and Avrll, to give pALK28.
- the 407 bp Mscl fragment missing from this construct was then excised from pALK24 (Example 16) and ligated into dephosphorylated, Mscl-digested pALK28.
- Clones were screened for the orientation of the insert, and a single clone containing the insert in the correct orientation was designated pALK32.
- pALK32 contains the required fragment to introduce the rap AT2 swap into module 3 of S.
- Example 18 Construction of a vector capable of over-expressing a crotonyl-CoA reductase
- pALK21 was constructed to over-express the crotonyl-CoA reductase (ccr) gene from Streptomyces cinnamonensis.
- pALK21 was constructed as described below.
- the S. cinnamonensis ccr gene was amplified by PCR from genomic DNA isolated from S. cinnamonensis ATCC 15413 using primers CCRMONF (SEQ LD NO:21) and CCRMONR (SEQ ID NO:22).
- CCRMONF introduces an Ndel site (bp 7-12 of SEQ ID NO:21) which incorporates the ATG of the start codon of the gene
- CCRMONR introduces a BamHl site (bp 6-11 of SEQ LD NO:22) downstream of the stop codon.
- Amplification to obtain the ccr gene was carried out under standard conditions using Pwo thermostable DNA polymerase.
- Plasmid pLSB46 contains the S. cinnamonensis ccr gene in the orientation which places the introduced BamHl site adjacent to the Xbal site of the polylinker.
- SEQ LD NO 23 shows the ccr gene from the Ndel site at the start codon to the BamHl site after the stop codon.
- the ccr gene was excised as a&NdeUXba fragment and cloned into the expression vector pLSB2 (Example 2) digested with Ndel and Xbal, to give pLSB59.
- Plasmid pLSB59 contains the ccr gene under control of the act! promoter, and the activator ⁇ ctZZ-ORF4.
- the ccr-containing fragment, along with the actII-ORF4 activator and actl promoter was transferred into a vector containing a second promoter (Pptr) which has restriction sites available for a hybrid PKS gene. This was achieved as described below.
- Plasmid pLSB59 was digested with Ndel, and the ends were filled-in using the Klenow fragment of DNA polymerase I. This blunt-end fragment was re-circularized to give pALK19.
- the gene and promoter were excised from pALK19 as a SpellXbal fragment and cloned into pLSB3 (Example 2) digested with Spel. This step destroys the_ ⁇ b l site at the end of the ccr gene. The resulting plasmid is designated pALK21.
- Plasmid pALK21 therefore contains the ccr gene under P ⁇ C with unique Ndel and Xbal sites situated for the introduction of hybrid PKS genes downstream of the ptr promoter.
- pALK21 is apramycin resistant and contains an o ⁇ for conjugal transfer of DNA.
- Example 19 Generation of a S. spinosa strain harbouring a hybrid polyketide synthase comprising the rapamycin module 2 AT in place of the spinosyn module 3 AT
- Saccharopolyspora spinosa NRRL 18538 was transformed by conjugation (Matsushima et al. 1994) from E. coli S17-1 (Simon et al., 1983) with pALK39. Transformants were selected for resistance to apramycin. A number of transformants were screened by Southern blot analysis. A single transformant, displaying the correct hybridization pattern to show that the plasmid had integrated into the chromosome by homologous recombination, was designated strain S. spinosa 7D23.
- Examples 20-21 hereinafter illustrate the use of S. spinosa 7D23 to produce novel spinosyns.
- 16-Desmethyl spinosyn A has previously been identified as one of the family of spinosyns produced by a number of S. spinosa strains, and designated spinosyn F (US 6,274,350 Bl). Here we demonstrated production of spinosyn F and 16-desmethyl spinosyn D (Compound 21) from the engineered hybrid pathway of S. spinosa 7D23.
- S. spinosa 7D23 was used to inoculate CSM medium (Hosted and Baltz 1996; US Pat. No. 5,362,634). This pre-culture was grown in a 250 mL flask with a 30 cm spring to aid aeration, shaken at 250 rpm with a two-inch throw, at 30°C with 75% relative hiimidity for 3 days. It was then used to inoculate vegetative medium, 3 x 30 mL cultures in 250 mL flasks (Strobel and Nakatsukasa 1993; US Pat. No. 5,362,634) at 5% v/v, which was cultured under the same conditions for a further 2 days.
- the vegetative culture was used to inoculate production medium (Strobel and Nakatsukasa 1993) at 5% v/v, 30 x 30 mL cultures in 250 mL flasks grown under the conditions described above.
- the production culture of S. spinosa 7D23 was grown for 10 days.
- the remaining fermentation broth was clarified by centrifugation.
- the cells were extracted twice with an equal volume of methanol.
- the supernatant (780 mL) was adjusted to pH-10 with 5 N NaOH and twice extracted with ethyl acetate (3 x 500 mL).
- the methanol and ethyl acetate extracts were combined and the solvents removed in vacuo to give an oily residue.
- the residue was dissolved into ethyl acetate (250 mL) and extracted with 50 mM tartaric acid (3 x 200 mL).
- the combined tartaric acid exfracts were adjusted to pH-10 with 5 N NaOH and extracted with ethyl acetate (3 x 300 mL).
- the extracts were combined and the solvent removed in vacuo to yield a brown oil (200 mg).
- the oil was dissolved into methanol (1 mL) and half of this was initially chromatographed as described in Example 8.
- 16-Desmethyl spinosyn D (Compound 21) has the following characteristics.
- Table 13 summarizes the ! H and 13 C ⁇ MR chemical shift data for 16-desmethyl spinosyn D in CDC1 3 .
- spinosyn F 17-pseudoaglycone was previously identified and is known as spinosyn F 17-pseudoaglycone. It is found as one of the minor members of the family of spinosyns produced by S. spinosa strains (US Pat. No. 6,274,350 Bl). Described below is the process for the production of spinosyn F 17-pseudoaglycone and 16-desmethyl spinosyn D 17-pseudoaglycone from the engineered hybrid pathway of S. spinosa 7D23.
- Frozen vegetative stocks of S. spinosa 7D23 were used to inoculate primary vegetative pre-cultures of S. spinosa 7D23 in CSM (50 mL incubated in a 250 mL Erlenmeyer flask with spring). Secondary pre-cultures in vegetative medium (250 mL incubated in a 2 L Erlenmeyer flask with spring) were prepared and incubated as described in Example 8.
- Production medium was inoculated with the secondary pre-culture at 5% v/v and was allowed to ferment in a 7 L stirred bioreactor for 7- 10 days at a temperature of 30°C. Airflow was set at 0.75 wm, and impeller tip speed was controlled between 0.68 and 1.1 ms "1 in order to maintain dissolved oxygen tension at or above 30% of air saturation.
- BASF Pluronic L-0101
- the remaining fermentation broth was clarified by centrifugation.
- the cells were twice extracted with an equal volume of methanol.
- the supernatant (3.2 L) was extracted with ethyl acetate (2 x 1.5 L).
- the methanol and ethyl acetate extracts were combined and the solvent removed in vacuo.
- the residual oil was dissolved into ethyl acetate (1 L) and washed with 50 mM tartaric acid (3 x 500 mL).
- the remaining ethyl acetate solution was evaporated in vacuo.
- the resulting oil was chromatographed over flash silica gel (6 x 13 cm) eluting with 5% methanol in chloroform. Fractions of 10 mL volume were collected.
- Table 15 summarizes the ! H and 13 C NMR spectral data for 16-desmethyl spinosyn D 17- pseudoagylcone in CDC1 3 .
- a hybrid spinosyn PKS was generated in which the methyl malonyl-CoA specific AT domain of module 3 was replaced by the ethyl malonyl-CoA specific AT domain of tylosin module 5 (SEQ LD NO:26).
- the producing strain was designated S. spinosa 36P4.
- Ethyl malonyl-CoA was not expected to be abundant in S. spinosa, so the S. cinnamonensis gene encoding crotonyl-CoA reductase was expressed in the same cell, under control of the act/ promoter.
- the PKS of S. spinosa 36P4 contained spnA, spnB and a truncated spnC under the native spnA promoter, followed by the plasmid DNA including the apramycin resistance marker.
- spnC hybrid spnC
- spnC* hybrid spnC
- Strain S. spinosa 36P4 produced minor components which had the UN absorbance, chromatographic properties, masses and fragmentation patterns consistent with the expected 16-desmethyl-l 6-ethyl spinosyns A and D.
- the major products from S. spinosa 36P4 were spinosyns A and D.
- the predominant novel fermentation products of strain 36P4 were 2 l-desethyl-21 -n-propyl spinosyn A and 6-ethyl spinosyn A.
- 2 l-desethyl-21 -n- propyl spinosyn A was produced at levels within 10% of that of spinosyn D.
- Example 22 Construction of a vector which can be used to engineer the spinosyn biosynthetic pathway to produce 16-desmethyl- 16-ethyl spinosyns
- Plasmid pTB4 is a pUC18-based plasmid containing a BamHl fragment of the tylosin PKS that includes most of the tylosin module 4.
- the insert is from the BamHl site between bp 24125 and 24130 of the deposited sequence tylG.embo, accession number U78289 (at the beginning of KS4) and the BamHl site between bp 31597 and 31612 (at the beginning of KS5).
- the tylosin module 5 AT was amplified by PCR using the template pTB4 and the primers AK1 (SEQ LD NO:24) and AK2 (SEQ ID NO:25).
- the primer AK1 introduces an Mscl site (bp 3-8 of SEQ ID NO:24) at the beginning of the AT domain and primer AK2 introduces an ⁇ vrll site (bp 3-8 of SEQ LD NO:25) at the end of the AT domain.
- the PCR reaction was carried out under standard conditions using Pwo thermostable DNA polymerase.
- the fragment was phosphorylated with T4 polynucleotide kinase and cloned into commercially-available pUC18 digested with Smal and dephosphorylated. Insert-containing plasmids were analysed for the orientation of the insert and sequenced. One plasmid containing the correct sequence was identified and designated pALK17. It contains the PCR fragment in the orientation which places the Mscl site adjacent to the Hindlll site of the polylinker. The tyl AT5 was excised from pALK17 as an MscllAvrll fragment and cloned into pALK26 digested with Mscl and ⁇ vrll to give pALK27.
- the 407 bp Mscl fragment which is missing from this construct was excised from pALK24 (Example 16) and ligated into dephosphorylated, Mscl-digested pALK27.
- a single clone containing the insert in the correct orientation was designated pALK31.
- pALK31 contains the required fragment to introduce the tyl AT5 swap into module 3 of S. spinosa, with an Ndel site at the start codon and a Xbal site just downstream of the polyketide synthase sequence.
- This fragment was excised as an NdellXbal fragment and cloned into pALK21 to give pALK36.
- Example 23 Generation of a S. spinosa strain harbouring a hybrid polyketide synthase comprising the tylosin module 5 AT in place of the spinosyn module 3 AT, and providing the appropriate ethyl malonyl-CoA co-substrate
- Saccharopolyspora spinosa NRRL 18538 was transformed with pALK36. Transformants were selected for resistance to apramycin and screened by Southern blot analysis. A single transformant was designated strain S. spinosa 36P4.
- Frozen vegetative stocks of S. spinosa 36P4 were inoculated into primary vegetative pre-cultures of S. spinosa 36P4 in CSM (50 mL incubated in a 250 mL Erlenmeyer flask with spring). Secondary pre-cultures in vegetative medium (250 mL incubated in a 2 L Erlenmeyer flask with spring) were prepared and incubated as described in Example 8.
- Production medium was inoculated with the secondary pre-culture at 5% v/v and was allowed to ferment in a 20 L stirred bioreactor for 7-10 days under conditions described in Example 8.
- several other new components were clearly present but were present in minor quantities.
- One of these new components eluted after the first major new component and had an identical mass to spinosyn D; this compound was probably 16-desmethyl-l 6-ethyl spinosyn A.
- the remaining fermentation broth (12 L) was clarified by centrifugation and extracted as described in Example 8. The residue was dissolved into methanol (10 mL), water (2 mL) and formic acid (100 ⁇ l). The whole sample was filtered and applied under gravity to a C 18 - BondElute SPE cartridge (70 g, 150 mL). The cartridge was then developed with an increasing 10%-stepwise gradient of acetonitrile in water (100 mL each step) containing formic acid at 0.1% using a FlashMaster Personal system (Jones Chromatography, Wales UK). The column was finally washed with methanol (2 x 100 mL).
- Fractions were collected ever 30 seconds. Fractions containing only 2 l-desethyl-21 -n-propyl spinosyn A were combined. Samples containing a mixture of this compound with spinosyn D were combined separately, the solvent removed in vacuo, and the residue chromatographed once again as described above. The fractions containing only 2 l-desethyl-21 -n-propyl spinosyn A were then combined with those from the first run. The fractions containing a mixture of the two compounds were combined and another round of chromatography performed.
- 6-Ethyl spinosyn A (Compound 24) has the following characteristics.
- Table 17 summarizes the l H and 13 C ⁇ MR chemical shift data for 6-ethyl spinosyn A in CDC1,
- Hybrid PKS genes constructed by the replacement of other AT (acyltransferase) domains within spn extender modules can be used to produce novel spinosyns with altered side chains at other positions on the polyketide.
- hybrid polyketide synthases can be constructed to yield spinosyns in which C18 or C20 bears a side chain other than a hydrogen (generally a methyl or ethyl).
- the native AT domains that incorporate predominantly methylmalonyl-CoA can be replaced by heterologous domains that preferentially incorporate malonyl-CoA (such as rapamycin AT2) or ethylmalonyl-CoA (such as tylosin AT5).
- malonyl-CoA such as rapamycin AT2
- ethylmalonyl-CoA such as tylosin AT5
- donor domains or modules for these hybrid polyketide synthases could be acquired from a variety of Type I polyketide synthase clusters and that this is not restricted in any way to domains or modules from erythromycin, avermectin, rapamycin and tylosin biosynthesis.
- the replacement of other spn AT domains could generate hybrid PKS genes which lead to the synthesis of spinosyns with an ethyl group at C6, or methyl or ethyl side chains at C18 or C20.
- the spn PKS domains responsible for the degree of modification of each beta-keto group might also be replaced by heterologous domains to generate hybrid PKS genes that result in spinosyns with different saturated bonds, hydroxyl groups or double bonds.
- hybrid spinosyn PKS genes claimed herein are useful for the production of novel, insecticidally-active spinosyns.
- the hybrid genes are derived from the spn PKS genes combined with a portion or portions of other Type I PKS genes.
- the strategies described in WO 98/01546 “Polyketides and their synthesis” were used to select the sites where the DNAs are spliced together.
- the hybrid genes can be operably linked to a heterologous promoter such as that from the actinorhodin biosynthetic gene actl (along with the ⁇ ct//-ORF4 gene encoding its cognate activator, see WO 98/01546), or from the pristinamycin resistance gene ptr (Blanc et al., 1995).
- the hybrid PKS genes are expressed in an organism which also contains the non-PKS functions required to produce a biologically-active spinosyn.
- the modified strains provided by the invention may be cultivated to provide spinosyns using conventional protocols such as those disclosed in U.S. Pat. No. 5,362,634.
- hybrid spinosyn PKSs of the invention can be expressed not only in Saccharopolyspora spinosa, but also in other host organisms, for example Saccharopolyspora erythaea, to produce insecticidally-active spinosyns.
- Other prokaryotic cells belonging to the group of actinomycetes, preferably the group of streptomycetes, are also suitable host organsims. Streptomyces albus is a specific example.
- the compounds claimed herein are useful for the control of insects and mites. Included are all isomers of the compounds, and any acid addition salts of the compounds and their isomers. Also included are semi-synthetic derivatives made by the methods described in U.S. Pat. No. 6,001,981 to prepare other modified spinosyns.
- the compounds show activity against a number of insects and mites. More specifically, the compounds show activity against members of the insect order Lepidoptera such as the beet armyworm, tobacco budworm, codling moth and cabbage looper. They also show activity against members of the order Coleoptera (the beetles and weevils) and Diptera (the true flies). The compounds also show activity against members of the order Hempitera (true bugs), Homoptera (aphids and hoppers), Thysanoptera (thrips), Orthoptera (cockroaches), Siphonaptera (fleas), Isoptera (termites), and members of the Hymenoptera order Formicidae (ants). The compounds also show activity against the two-spotted spider mite, which is a member of the Arachnid order Acarina.
- a further aspect of the present invention is directed to methods for inhibiting an insect or mite.
- the present invention is directed to a method for inhibiting a susceptible insect that comprises applying to a plant an effective insect- inactivating amount of compound in accordance with the present invention.
- the claimed compounds are applied in the form of compositions, which are also a part of this invention. These compositions comprise an insect- or mite-inactivating amount of compound in an inert carrier.
- the active component may be present as a single claimed compound, a mixture of two or more compounds or a mixture of any of the compounds together with the dried portion of the fermentation medium in which it is produced.
- compositions are prepared according to the procedures and formulas which are conventional in the agricultural or pest control art, but which are novel and important because of the presence of one or more of the compounds of this invention.
- the compositions may be concentrated formulations, which are dispersed in water or may be in the form of a dust, bait or granular formulation used without further treatment.
- compositions according to the invention can be broadened considerably by adding other, for example insecticidally, acaricidally, and/or nematocidally active, ingredients.
- insecticidally for example, one or more of the following compounds can suitably be combined with the compounds of the invention: organophosphorus compounds, carbamates, pyrethroids, acylureas, other types of insect growth regulators and insect hormone analogs, neonicotinoids and other nicotinics, macrolides and other insecticidal, acaricidal, mollscicial and nematocidal compounds or actives.
- WO 00/56156 on “Synergistic Insecticide Mixtures” discloses use of certain previously known spinosyn compounds in combination with agonists or antagonists of nicotinic acetylcholine receptors to control animal pests.
- WO 00/35282 on “Combination of Active Ingredients” discloses use of spinosad in combination with a fungicidally active compound.
- WO 00/35286 on “Combinations of Active Ingredients” discloses use of a combination of spinosad with other compounds to control animal pests and fungi.
- WO 99/60856 on “Use of Spinosyns as Soil Insecticides” discloses use of certain previously known spinosyns for treating seeds and for application to plants via the soil or by irrigation to control insects.
- WO 99/33343 on “Use of Macrolides in Pest Control” discloses use of spinosyns to control pests in transgenic crops, use of spinosyns to protect plant propagation material and plant organs formed at a later time from attack by pests, and use of spinosyns to control wood pests and molluscs.
- the compounds of Formula I can also be used for these purposes.
- the compounds of the present invention are also useful for the freatment of animals to control arthropods, i.e., insects and arachnids including various flies and fly larvae, fleas, lice, mites, and ticks, which are pests on animals.
- arthropods i.e., insects and arachnids including various flies and fly larvae, fleas, lice, mites, and ticks, which are pests on animals.
- Techniques for delivering ectoparasiticides are well known to those skilled in the art.
- a present compound is applied to the exterior surface of an animal by sprays, dips or dusts.
- the compounds can also be delivered to animals using ear tags, a delivery method disclosed in US Pat. No. 4,265,876.
- the compounds can be used to control insects and arachnids which are pests in the feces of cattle and other animals.
- the compounds are administered orally and the compounds travel through the intestinal tract and emerge in the feces. Control of pests in the feces indirectly protects the animals from the pests.
- the compounds of the invention are also useful as human pharmaceuticals to control parasites, for example, lice.
- the compounds can be used, for example, in the formulations for controlling lice that are disclosed in WO 00/01347.
- Biological activity of the compounds of the invention was shown by a topical assay in which the compound was applied to laboratory-reared larvae (mean weight 22 mg) at the rate of 1 microg/larva. Each compound was applied, in an acetone solution (1 mg/mL), along the dorsum of six tobacco budworm (Heliothis virescens) larvae and six beet armyworm (Spodoptera exigua) larvae. Treated larvae were then held for two days at 21°C, 60% RH in six-well plastic culture plates. Larvae were each supplied with a 1 cm 3 of agar-based lepidoptera diet for sustenance during the two-day post-exposure interval. Percent mortality was determined at the end of a two-day period (Table 18).
- the compounds of formula (I) can be used as intermediates in the processes disclosed in US 6,001,981 to produce semi-synthetic spinosyn analogues, which are also expected to have insecticidal activity.
Abstract
Description
Claims
Priority Applications (12)
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MXPA04008071A MXPA04008071A (en) | 2002-02-19 | 2003-02-19 | Novel spinosyn-producing polyketide synthases. |
CN038041626A CN1633237B (en) | 2002-02-19 | 2003-02-19 | Novel SPINOSYN-producing polyketide synthases |
DK03713546T DK1492404T3 (en) | 2002-02-19 | 2003-02-19 | New spin-forming polyketide synthases |
JP2003569801A JP4765032B2 (en) | 2002-02-19 | 2003-02-19 | Polyketide synthase producing a novel spinosyn |
AU2003217591A AU2003217591B2 (en) | 2002-02-19 | 2003-02-19 | Novel spinosyn-producing polyketide synthases |
EP20030713546 EP1492404B1 (en) | 2002-02-19 | 2003-02-19 | Novel spinosyn-producing polyketide synthases |
ES03713546T ES2375714T3 (en) | 2002-02-19 | 2003-02-19 | NEW POLYQUÉTIDO-SYINTETASAS PRODUCERAS DE ESPINOSINA. |
KR1020047012851A KR101016561B1 (en) | 2002-02-19 | 2003-02-19 | Novel spinosyn-producing polyketide synthases |
IL16352903A IL163529A0 (en) | 2002-02-19 | 2003-02-19 | Novel spinosyn-producing polyketidesynthases |
IL16352904A IL163529A (en) | 2002-02-19 | 2004-08-12 | Spinosyn-producing polyketide synthases |
AU2009200685A AU2009200685B2 (en) | 2002-02-19 | 2009-02-20 | Novel spinosyn-producing polyketide synthases |
IL200471A IL200471A (en) | 2002-02-19 | 2009-08-18 | Spinosyn-producing polyketide synthases |
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CN (1) | CN1633237B (en) |
AU (2) | AU2003217591B2 (en) |
DK (1) | DK1492404T3 (en) |
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US9404107B2 (en) | 2011-05-03 | 2016-08-02 | Dow Agrosciences Llc | Integration of genes into the chromosome of Saccharopolyspora spinosa |
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Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
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Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4265876A (en) | 1978-03-07 | 1981-05-05 | Shell Internationale Research Maatschappij B.V. | Animal ear-tags, their manufacture and their use |
EP0375316A1 (en) | 1988-12-19 | 1990-06-27 | DowElanco | Macrolide compounds |
WO1993009126A1 (en) | 1991-11-08 | 1993-05-13 | Dowelanco | New a83543 compounds and process for production thereof |
WO1993013663A1 (en) | 1992-01-17 | 1993-07-22 | Abbott Laboratories | Method of directing biosynthesis of specific polyketides |
WO1994020518A1 (en) | 1993-03-12 | 1994-09-15 | Dowelanco | New a83543 compounds and process for production thereof |
US5362634A (en) | 1989-10-30 | 1994-11-08 | Dowelanco | Process for producing A83543 compounds |
WO1998001571A2 (en) | 1996-07-05 | 1998-01-15 | Biotica Technology Limited | Erythromycins and process for their preparation |
WO1999033343A2 (en) | 1997-12-23 | 1999-07-08 | Novartis Ag | Use of macrolides in pest control |
WO1999046387A1 (en) | 1998-03-09 | 1999-09-16 | Dow Agrosciences Llc | Biosynthetic genes for spinosyn insecticide production |
WO1999060856A1 (en) | 1998-05-26 | 1999-12-02 | Bayer Aktiengesellschaft | Use of spinosynes as soil insecticides |
US6001981A (en) | 1996-06-13 | 1999-12-14 | Dow Agrosciences Llc | Synthetic modification of Spinosyn compounds |
WO2000001347A2 (en) | 1998-07-02 | 2000-01-13 | Eli Lilly And Company | Formulations for controlling human lice |
WO2000035282A1 (en) | 1998-12-16 | 2000-06-22 | Bayer Aktiengesellschaft | Combination of active agents |
WO2000035286A1 (en) | 1998-12-16 | 2000-06-22 | Bayer Aktiengesellschaft | Combinations of active ingredients |
WO2000056156A1 (en) | 1999-03-24 | 2000-09-28 | Bayer Aktiengesellschaft | Synergistic insecticide mixtures |
WO2001019840A1 (en) | 1999-09-13 | 2001-03-22 | Dow Agrosciences Llc | Pesticidal macrolides |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5824513A (en) * | 1991-01-17 | 1998-10-20 | Abbott Laboratories | Recombinant DNA method for producing erythromycin analogs |
US5539089A (en) * | 1991-11-08 | 1996-07-23 | Dowelanco | A83543 aglycones and pseudoglycones |
US5591606A (en) * | 1992-11-06 | 1997-01-07 | Dowelanco | Process for the production of A83543 compounds with Saccharopolyspora spinosa |
CA2197524A1 (en) * | 1996-02-22 | 1997-08-22 | Bradley Stuart Dehoff | Polyketide synthase genes |
JP3441899B2 (en) * | 1996-11-01 | 2003-09-02 | 理化学研究所 | How to make a full-length cDNA library |
GB9814006D0 (en) * | 1998-06-29 | 1998-08-26 | Biotica Tech Ltd | Polyketides and their synthesis |
GB9814622D0 (en) * | 1998-07-06 | 1998-09-02 | Biotica Tech Ltd | Polyketides,their preparation,and materials for use therein |
WO2000020601A2 (en) * | 1998-10-02 | 2000-04-13 | Kosan Biosciences, Inc. | Polyketide synthase enzymes and recombinant dna constructs therefor |
JP2002535387A (en) * | 1999-01-27 | 2002-10-22 | コーサン バイオサイエンシーズ, インコーポレイテッド | Synthesis of oligoketides |
JP2000245457A (en) * | 1999-02-24 | 2000-09-12 | Kitasato Inst:The | Avermectin aglycon synthetase |
GB9912563D0 (en) * | 1999-05-28 | 1999-07-28 | Biotica Tech Ltd | Polyketides and their synthesis |
WO2001016303A2 (en) * | 1999-08-27 | 2001-03-08 | Bayer Aktiengesellschaft | Nucleic acids which code for the enzyme activities of the spinosyn biosynthesis |
WO2002007705A1 (en) * | 2000-07-20 | 2002-01-31 | Campina B.V. | Carrier material for dry powder inhalation |
GB0019986D0 (en) * | 2000-08-14 | 2000-10-04 | Biotica Tech Ltd | Polyketides and their synthesis |
US6919464B1 (en) * | 2001-03-21 | 2005-07-19 | Dow Agrosciences Llc | Synthetic derivatives of 21-butenyl and related spinosyns |
TWI275592B (en) * | 2001-03-21 | 2007-03-11 | Dow Agrosciences Llc | Synthetic derivatives of 21-butenyl and related spinosyns |
DE10135550A1 (en) * | 2001-07-20 | 2003-01-30 | Bayer Cropscience Ag | Process for the preparation of new spinosyn derivatives |
JP4765032B2 (en) * | 2002-02-19 | 2011-09-07 | ダウ・アグロサイエンス・エル・エル・シー | Polyketide synthase producing a novel spinosyn |
WO2005109887A1 (en) * | 2004-04-23 | 2005-11-17 | Smart Metric, Inc. | Smartcard with visual display |
-
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Patent Citations (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4265876A (en) | 1978-03-07 | 1981-05-05 | Shell Internationale Research Maatschappij B.V. | Animal ear-tags, their manufacture and their use |
EP0375316A1 (en) | 1988-12-19 | 1990-06-27 | DowElanco | Macrolide compounds |
US5362634A (en) | 1989-10-30 | 1994-11-08 | Dowelanco | Process for producing A83543 compounds |
WO1993009126A1 (en) | 1991-11-08 | 1993-05-13 | Dowelanco | New a83543 compounds and process for production thereof |
WO1993013663A1 (en) | 1992-01-17 | 1993-07-22 | Abbott Laboratories | Method of directing biosynthesis of specific polyketides |
WO1994020518A1 (en) | 1993-03-12 | 1994-09-15 | Dowelanco | New a83543 compounds and process for production thereof |
US5670486A (en) | 1993-03-12 | 1997-09-23 | Dowelanco | A83543 compounds and process for production thereof |
US6001981A (en) | 1996-06-13 | 1999-12-14 | Dow Agrosciences Llc | Synthetic modification of Spinosyn compounds |
WO1998001571A2 (en) | 1996-07-05 | 1998-01-15 | Biotica Technology Limited | Erythromycins and process for their preparation |
WO1998001546A2 (en) | 1996-07-05 | 1998-01-15 | Biotica Technology Limited | Polyketides and their synthesis |
WO1999033343A2 (en) | 1997-12-23 | 1999-07-08 | Novartis Ag | Use of macrolides in pest control |
WO1999046387A1 (en) | 1998-03-09 | 1999-09-16 | Dow Agrosciences Llc | Biosynthetic genes for spinosyn insecticide production |
US6143526A (en) | 1998-03-09 | 2000-11-07 | Baltz; Richard H. | Biosynthetic genes for spinosyn insecticide production |
US6274350B1 (en) | 1998-03-09 | 2001-08-14 | Dow Agrosciences Llc | Biosynthetic genes for spinosyn insecticide production |
WO1999060856A1 (en) | 1998-05-26 | 1999-12-02 | Bayer Aktiengesellschaft | Use of spinosynes as soil insecticides |
WO2000001347A2 (en) | 1998-07-02 | 2000-01-13 | Eli Lilly And Company | Formulations for controlling human lice |
WO2000035282A1 (en) | 1998-12-16 | 2000-06-22 | Bayer Aktiengesellschaft | Combination of active agents |
WO2000035286A1 (en) | 1998-12-16 | 2000-06-22 | Bayer Aktiengesellschaft | Combinations of active ingredients |
WO2000056156A1 (en) | 1999-03-24 | 2000-09-28 | Bayer Aktiengesellschaft | Synergistic insecticide mixtures |
WO2001019840A1 (en) | 1999-09-13 | 2001-03-22 | Dow Agrosciences Llc | Pesticidal macrolides |
Non-Patent Citations (21)
Title |
---|
"Pesticide Manual", pages: 1272 |
BIERMAN, M., LOGAN, R., O'BRIEN, K., SENO, ET., NAGARAJA RAO, R., SCHONER, BE: "Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp", GENE, vol. 116, 1992, pages 43 - 49, XP023542508, DOI: doi:10.1016/0378-1119(92)90627-2 |
BISANG, C., LONG, PF., CORTES, J., WESTCOTT, J., CROSBY, J., MATHARU, AL., COX, RJ., SIMPSON, TJ., STAUNTON, J., LEADLAY, PF.: "A chain initiation factor common to both modular and aromatic polyketide synthases", NATURE, vol. 401, 1999, pages 502 - 505, XP002130648, DOI: doi:10.1038/46829 |
BROUGHTON, MC., HUBER, MLB., CREEMER, LC., KIRST, HA., TURNER JR.: "Biosynthesis of the macrolide insecticidal compound A83543 by Saccharopolyspora spinosa", PROCEEDINGS OF AMER. SOC. MICROBIOL., 1991 |
DONADIO, S., STAVER, MJ., MCALPINE, JB., SWANSON, SJ., KATZ, L.: "Modular organization of genes required for complex polyketide biosynthesis", SCIENCE, vol. 252, 1991, pages 675 - 679, XP002035890, DOI: doi:10.1126/science.2024119 |
DONADIO, SA., STASSI, D., MCALPINE, JB., STAVER, MJ., SHELDON, PJ., JACKSON, M., SWANSON, SJ., WENDT-PIENKOWSKI, E., WANG, YG., JA: "Industrial microorganisms: basic and applied molecular genetics", 1993, AMER. SOC. MICROBIOL, article "Recent developments in the genetics of erythromycin formation", pages: 257 - 265 |
DUTTON, CJ., GIBSON, SP., GOUDIE, AC., HOLDOM, KS., PACEY, MS., RUDDOCK, JC., BU'LOCK, JD., RICHARDS, MK: "Novel avermectins produced by mutational biosynthesis", J. ANTIBIOT., vol. 44, 1991, pages 357 - 365, XP002079430 |
HOSTED, TJ., BALTZ, RH.: "Mutants of Streptomyces roseosporus that express enhanced recombination within partially homologous genes", MICROBIOLOGY, vol. 142, 1996, pages 2803 - 2813 |
HUNZIKER ET AL., AMYCOLATOPSIS MEDITERRANEI, 1998 |
HUNZIKER, D., YU, TW., HUTCHINSON, CR., FLOSS, HG., KHOSLA, C.: "Primer unit specificity in rifamycin biosynthesis principally resides in the later stages of the biosynthetic pathway", J. AM. CHEM. SOC., vol. 12, 1998, pages 1092 - 1093, XP002951249, DOI: doi:10.1021/ja9736368 |
KIRST, HA., MICHEL, KH., MARTIN, JW., CREEMER, LC., CHIO, EH., YAO, RC., NAKATSUKASA, WM., BOECK, LD., OCCOLOWITZ, JL., PASCHAL, J: "A83543A-D, unique fermentation-derived tetracyclic macrolides", TETRAHEDRON LETTS, vol. 32, 1991, pages 4839 - 4842, XP000944455, DOI: doi:10.1016/S0040-4039(00)93474-9 |
MARSDEN, AFA., WILKINSON, B., CORTES, J., DUNSTER, NJ., STAUNTON, J., LEADLAY, PF.: "Engineering broader specificity into an antibiotic-producing polyketide synthase", SCIENCE, vol. 279, 1998, pages 199 - 202, XP002941701, DOI: doi:10.1126/science.279.5348.199 |
MATSUSHIMA, P., BROUGHTON, MC., TURNER, JR., BALTZ, RH.: "Conjugal transfer of cosmid DNA from Escherichia coli to Saccharopolyspora spinosa: effects of chromosomal insertions on macrolide A83543 production", GENE, vol. 146, 1994, pages 39 - 45, XP023541380, DOI: doi:10.1016/0378-1119(94)90831-1 |
PACEY, MS., DIRLAM, JP., GELDART, RW., LEADLAY, PF., MCARTHUR, HAI, MCCORMICK, EL., MONDAY, RA., O'CONNELL, TN, STAUNTON, J., WINC: "Novel erythromycins from a recombinant Saccharopolyspora erythraea strain NRRL 2338 pIGI. Fermentation, isolation and biological activity", J. ANTIBIOT., vol. 51, 1998, pages 1029 - 1034, XP002946835 |
ROWE, CJ., CORTES, J., GAISSER, S., STAUNTON, J., LEADLAY, PF.: "Construction of new vectors for high-level expression in actinomycetes", GENE, vol. 216, 1998, pages 215 - 223, XP004149299, DOI: doi:10.1016/S0378-1119(98)00327-8 |
SALAH-BEY, K., BLANC, V., THOMPSON, CJ.: "Stress-activated expression of a Streptomyces pristinaespiralis multidrug resistance gene (ptr) in various Streptomyces spp. and Escherichia coli", MOL. MICROBIOL., vol. 17, 1995, pages 1001 - 1012, XP008009342, DOI: doi:10.1111/j.1365-2958.1995.mmi_17051001.x |
See also references of EP1492404A4 |
SIMON, R., PREIFER, U., PÜHLER, A.: "A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria", BIOLTECHNOLOGY, vol. 1, 1983, pages 784 - 791, XP009009828, DOI: doi:10.1038/nbt1183-784 |
STASSI, DL., KAKAVAS, SJ, REYNOLDS, KA, GUNAWARDANA, G., SWANSON, S., ZEIDNER, D., JACKSON, M., LIU, H., BUKO, A., KATZ, L.: "Ethyl-substituted erythromycin derivatives produced by directed metabolic engineering", PROC. NATL. ACAD. SCI. USA, vol. 95, 1998, pages 7305 - 7309, XP002143632, DOI: doi:10.1073/pnas.95.13.7305 |
STROBEL, RJ., NAKATSUKASA WM.: "Response surface methods for optimizing Saccharopolyspora spinosa, a novel macrolide producer", J. INDUST. MICROBIOL., vol. 11, 1993, pages 121 - 127 |
WALDRON, C., MATSUSHIMA, P., ROSTECK, PR., JR., BROUGHTON, MC., TURNER, J., MADDURI, K., CRAWFORD, KP., MERLO, DJ., BALTZ, RH.: "Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa", CHEM. BIOL., vol. 8, 2001, pages 487 - 499, XP001008017, DOI: doi:10.1016/S1074-5521(01)00029-1 |
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Also Published As
Publication number | Publication date |
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AU2003217591A1 (en) | 2003-09-09 |
JP2005520500A (en) | 2005-07-14 |
CN1633237A (en) | 2005-06-29 |
AU2003217591B2 (en) | 2009-02-26 |
IL163529A (en) | 2011-11-30 |
CN1633237B (en) | 2010-05-26 |
JP4765032B2 (en) | 2011-09-07 |
US20060040877A1 (en) | 2006-02-23 |
US9137992B2 (en) | 2015-09-22 |
EP1492404A4 (en) | 2010-03-17 |
IL163529A0 (en) | 2005-12-18 |
EP1492404A2 (en) | 2005-01-05 |
US8624009B2 (en) | 2014-01-07 |
US20100068785A1 (en) | 2010-03-18 |
AU2009200685A1 (en) | 2009-03-12 |
US20110178036A1 (en) | 2011-07-21 |
KR101016561B1 (en) | 2011-02-22 |
AU2009200685B2 (en) | 2012-01-12 |
DK1492404T3 (en) | 2012-04-23 |
IL200471A (en) | 2015-09-24 |
WO2003070908A3 (en) | 2004-10-28 |
US7626010B2 (en) | 2009-12-01 |
EP1492404B1 (en) | 2011-12-28 |
ES2375714T3 (en) | 2012-03-05 |
MXPA04008071A (en) | 2004-11-26 |
US20030225006A1 (en) | 2003-12-04 |
US20140080777A1 (en) | 2014-03-20 |
KR20040085201A (en) | 2004-10-07 |
IL200471A0 (en) | 2011-07-31 |
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