WO2003068916A2 - Methods and apparatus for whole cell panning - Google Patents
Methods and apparatus for whole cell panning Download PDFInfo
- Publication number
- WO2003068916A2 WO2003068916A2 PCT/US2003/004001 US0304001W WO03068916A2 WO 2003068916 A2 WO2003068916 A2 WO 2003068916A2 US 0304001 W US0304001 W US 0304001W WO 03068916 A2 WO03068916 A2 WO 03068916A2
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- WIPO (PCT)
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- cells
- population
- container
- membrane
- open end
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
Definitions
- This disclosure relates to methods and apparatus for whole cell panning using phage/phagemid libraries against homogeneous cell cultures
- phage or phagemid particle display pre-constructed antibody or antibody fragment libraries are attached to phage coat protein genes of phage/phagemid. The antibody fragments are then expressed and displayed on the surface of the phage/phagemid particle. Antibodies with an affinity for a target antigen are captured, propagated in bacteria, and further enriched through successive rounds of selection.
- This technique as reviewed ' in Phage Display of Proteins and Peptides: A Laboratory Manual (Siegel, 2001), is referred to as panning. In most cases, panning methods use purified antigen immobilized to a solid support.
- Purification of the target antigen is often not possible either because the antigen is unknown, as with putative stem-cell markers or tumor-specific antigens, or the antigen cannot be produced in sufficiently large quantities. Also, purification of some membrane targets (i.e. seve ⁇ - transmembrane receptors) will likely cause improper folding in solution. In addition, purification procedures may affect an antigen's native binding properties. For these reasons, ' it is desirable to pan for antibody-bearing phage/phagemid particles that bind an antigen in its native state, such as on intact cell surfaces.-
- Non-specific binding of phage/phagemid tends to be a problem in all types of panning. This is due to a variety of . reasons including using complex antigens with "sticky surfaces". In whole cell based panning techniques, this problem is amplified by the presence of undesirable non-specific antigens or ligands presented on the cell surface. These antigens may take the form of endogenous proteins, carbohydrates, and lipids.
- the problems associated with non-specific binding in cell based panning techniques can be partially circumvented by employing a negative selection, in which the phage/phagemid libraries are pre-incubated with cells lacking the antigen of interest The unbound phage/phagemid is then used as the starting library for panning the target cells.
- Negative selection schemes are rather inefficient due to the low affinity of the non-specific binding interaction. Additionally, weak specific interactions between phage/phagemid and target antigen can be masked by negative selection, quickly depleting phage/phagemid libraries and reducing the chances of selecting for novel antibody-antigen interactions.
- one methods employs flow cytometry and fluorochrome-labeled antibodies that recognize an orthogonal antigen on the target ceil (See, U.S. Pat. No. 6,265,150 Bl). This method is predicated on the existence of a marker antigen on the target cell which distinguishes it from the adsorber cells and a fluorochrome-labeled antibody specific for it, a requirement that is not ' always easily attained, especially in tumor cell targets.
- Another method commonly used labels the target cells with magnetic beads followed by mixing with adsorber cells See, U.S. Pat. No. 5,876,925.
- Bacteriophage encoding Ig fragments are panned against a mixture of antigen-positive, biotinylated- cell targets pre-coated with streptavidin-conjugated magnetic microbeads and an excess of unlabeled antigen-negative cells. After phage incubation, the cells are separated through the use of a magnetic affinity column.
- One disadvantage with this technique is that the panning of Ig fragments is done against a non-native cell surface, which displays biotin/streptavidin/magnetic bead complexes.
- target cells with bound phage can be agglutinated from a heterogeneous mixture using immunoprecipitation (See, U.S. Pat No. 5,985,543).
- Whole cell panning using the above methods has several disadvantages. After panning with the phage library, the target cell populations must be separated from the non-specific adsorber cells. This requires that the target cells in the heterogeneous sample have markers on the cell surface which distinguish them from adsorber cells and that secondary antibodies that recognize these markers are available.
- the act of adding components to detect the markers on the cell surface of the target cells could perturb the native environment of the antigens on the cell surface, resulting in selection for antibodies that bind antigens displayed on tumor cells with a magnetic bead complex attached.
- the antibody-antigen interaction may be disrupted due to the process of separation (e.g. shear forces).
- a new method and apparatus for whole cell panning employs homogenous populations of cells separated by a membrane(s) permeable to phage/phagemid particles.
- One population of cells e.g., target cells
- a second population of cells is maintained on a second side of the membrane and consists of non-specific absorber cells. At no point in the protocol do the two cell populations mix.
- the ceil culture apparatus maintains the separation of two or more cell lines in two or more different chambers by one or more removable barriers.
- the isolated chambers allow the separate growth of non-specific cells and target antigen-positive cells.
- the semi-permeable membrane can be sandwiched between two solid barriers. If the different cells lines require different growth media, they can be placed into different chambers and grown with the solid pieces in place, preventing the mixture of any material, between the chambers. After growth is completed, the solid barriers are removed. When the barrier between the chambers is removed, the cell populations both contact a semi-permeable membrane that allows passive lateral diffusion among adjacent chambers.
- the membrane has a pore size that prevents cells from passing therethrough, but allows smaller particles (e.g., phage or phagemid) to pass through.
- a cell-type specific library of phage/phagemid particles is then added.
- the membrane allows the lateral diffusion of the phage/phagemid particles carrying antibody fragments between the chambers of the apparatus while keeping the cell lines separate.
- the entire apparatus can be loosely covered with a lid to allow air circulation.
- individual culture chambers having openings at both the top and side.
- the side opening accommodates a solid or semi-permeable insert.
- the individual chambers are provided with solid side inserts ail ⁇ jwjng for isolated cell growth.
- the solid side inserts can be removed and the two chambers can be attached onto either side of a semi-permeable membrane insert.
- the phage/phagemid library can then be added to one or both of the cell populations.
- the semi-permeable membrane allows the passive diffusion of the phage/phagemid particles between the chambers while keeping the adsorber and target cell types separate.
- the entire apparatus can be loosely covered with a lid to allow air circulation.
- Fig. 1 is a cross-sectional view of a whole cell panning apparatus in accordance with this disclosure having the solid barriers in place.
- Fig. 2 is a cross-sectional view of the whole cell panning apparatus of Fig. 1 having the solid barriers removed.
- Fig. 3 is a cross-sectional view of two individual chambers useful in assembling a whole cell panning apparatus in accordance with this disclosure.
- Fig.4 is a cross-sectional view of the two individual chambers of Fig. 3 as assembled to provide a whole cell panning apparatus in accordance with this disclosure.
- Fig. 5 is a cross-sectional view of an alternative embodiment of two individual chambers useful in assembling a whole cell panning apparatus in accordance with this disclosure having the solid barriers in place.
- a new apparatus 10 for whole cell panning includes a container 11 defining a first chamber 12 and a second chamber 22.
- the container 11 can be made from any inert material capable of supporting a solution of cells. Suitable materials of construction include, but are not limited to, glass and polymers, such as, for example, polycarbonate or polystyrene. Preferably the material of construction is transparent.
- Chamber 12 holds a first suspension 13 containing a population of cells (e.g., target cells).
- Chamber 22 holds a second suspension 23 containing a population of cells (e.g., absorber cells).
- a loosely fitting cover 16 prevents contamination of suspensions 13, 23.
- One side of chamber 12 is defined by a solid, removable insert 14.
- a solid, removable insert 24 One side of chamber 22 is defined by a solid, removable insert 24.
- Solid, removable inserts 14, 24 create a watertight seal so that mixing of the contents of chambers 12, 22 is prevented.
- Sandwiched between solid, removable inserts 14, 24 is a semi-permeable membrane 15.
- the semi-permeable membrane is designed to have openings that are too small to allow cells to move across the membrane.
- the pores of membrane 15 must be large enough, however to allow the passage of the display library through the membrane.
- the pore size of the membrane should be in the range of 0.2 to 7 microns, preferably about 5 microns.
- the membrane can be made of any inert material to which pores of the appropriate size can be imparted.
- a display library is added to one or both of containers 12, 22.
- the individual members of the library are free to pass through the membrane 15.
- Certain members of the display library will bind preferentially to the target cells in the first suspension 13. After a period of time, any binding that will take place has already taken place and the first suspension 13 can be removed and the binding members of the library can be removed from the target cells and recovered.
- the second suspension 23 and non-bound members of the library can be discarded.
- FIG. 3 An alternative embodiment of a whole cell panning apparatus is shown in Fig. 3, wherein chambers 112 and 122 are defined by separate containers 111 , 121 each of which has a cover 116, 126.
- Chamber 112 holds a first suspension 13 containing a population of cells (e.g., target cells).
- Chamber 122 holds a second suspension 123 containing a population of cells (e.g., absorber cells).
- the top of each container 111 , 121 has a threaded portion 117, 127 respectively.
- the covers 116, 126 can be screwed on tightly to avoid spillage during handling or transport, but can be left on loosely to allow air circulation during cell culturing.
- a display library is added to one or both suspensions 113, 123, and a common cover 130 is applied to containers 111 , 121.
- the common cover 130 has a first threaded portion 135 which can be screwed tightly to threaded portion 117 of container 111 and a second threaded portion 137 which can be screwed tightly to threaded portion 127 of container 121.
- Membrane 15 defines the center portion of the common cover 130.
- the two populations of cells remain distinct.
- the individual members of the library are free to pass through the membrane 115.
- Certain members of the display library will bind preferentially to the target cells in the first suspension 113. After a period of time, any binding that will take place has already taken place and the first suspension 113 can be removed and the binding members of the library can be removed from the target cells and recovered. The second suspension 123 and non-bound members of the library can be discarded.
- chambers 212 and 222 are defined by separate containers 211, 221 in the shape of rectangular test-tubes. Each container 211 , 221 has cover a top 216, 226, and a water tight side cover 218, 228 which snap fits onto side flanges 219, 229, respectively, so as to cover membranes 215, 225.
- Chamber 212 holds a first suspension 213 containing a population of cells (e.g., target cells).
- Chamber 222 holds a second suspension 223 containing a population of cells (e.g., absorber cells).
- suspension 213 is in direct contact with membrane 215 and suspension 223 is in direct contact with membrane 225.
- a display library is added to one or both suspensions 213, 223.
- Side covers 218, 228 are removed and a joining sleeve 230 is applied to containers 211 , 221 in place of side covers 218, 228.
- the joining sleeve 230 connects the two containers 21 1 , 221 so that fluid and library members (but not cells) can pass from one chamber 212 to the other 222.
- the pores of the membranes 215 and 225 are too small to permit the passage of cells, the two populations of cells remain distinct.
- the individual members of the library are free to pass through the membranes 215, 225.
- Certain members of the display library will bind preferentially to the target cells in the first suspension 213. After a period of time, any binding that will take place has already taken place and the first suspension 213 can be removed and the binding members of the library can be removed from the target cells and recovered. The second suspension 223 and non-bound members of the library can be discarded.
- a single membrane on joining sleeve 230 instead of a membrane on each container.
- stirring e.g., via a magnetic st ⁇ rrer
- pumping can be advantageously employed to assist in moving members of the library through the membrane in any of the previously described embodiments. Pumping may also help in the event a washing step is to be performed while the cell suspensions are still located within the chambers of the apparatus. Suitable types of pumps include, but are not limited to, peristaltic pumps. It is also contemplated that the two syringe-like containers may be employed in which case one or more syringe plungers can be used manually to provide a pumping action.
- the method of whole cell panning described herein retains the benefits of the existing methods while eliminating the major drawbacks. Because the target cells and absorber cells are kept as separate homogenous populations in different chambers of the apparatus at all times, a ceil separation step is not required, therefore no cell markers are needed.
- cell lines can be grown in different media using solid separators. These solid separators can be removed exposing a semi-permeable membrane. Phage/phagemid libraries are then added. The phage/phagemid particles are able to move freely through the membrane to both chambers while the different cell types are kept separate.
- the containers may be covered with what are commonly known to those skilled in the art as "snap caps". These "snap caps” have a first position in which the cap is loosely on the container and a second snapped-down position which forms a water-tight seal.
- the watertight side covers in the embodiment shown in Fig. 5 can be replaced with peel-away covers to provide a one time use device for cell panning.
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- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
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- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
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Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003568031A JP2005526498A (en) | 2002-02-11 | 2003-02-10 | Method and apparatus for whole cell panning |
AU2003210949A AU2003210949A1 (en) | 2002-02-11 | 2003-02-10 | Methods and apparatus for whole cell panning |
EP03739727A EP1570053A2 (en) | 2002-02-11 | 2003-02-10 | Methods and apparatus for whole cell panning |
CA002476237A CA2476237A1 (en) | 2002-02-11 | 2003-02-10 | Methods and apparatus for whole cell panning |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35608802P | 2002-02-11 | 2002-02-11 | |
US60/356,088 | 2002-02-11 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003068916A2 true WO2003068916A2 (en) | 2003-08-21 |
WO2003068916A3 WO2003068916A3 (en) | 2005-07-14 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/004001 WO2003068916A2 (en) | 2002-02-11 | 2003-02-10 | Methods and apparatus for whole cell panning |
Country Status (5)
Country | Link |
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EP (1) | EP1570053A2 (en) |
JP (1) | JP2005526498A (en) |
AU (1) | AU2003210949A1 (en) |
CA (1) | CA2476237A1 (en) |
WO (1) | WO2003068916A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006055371A2 (en) * | 2004-11-16 | 2006-05-26 | Alexion Pharmaceuticals, Inc. | Functional screening of antibody libraries |
CN109906267A (en) * | 2016-10-12 | 2019-06-18 | 连王·罗斯 | Miniature organism reactor assemblies |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6737266B1 (en) * | 1999-10-01 | 2004-05-18 | 3M Innovative Properties Company | Devices and methods for microorganism detection |
-
2003
- 2003-02-10 AU AU2003210949A patent/AU2003210949A1/en not_active Abandoned
- 2003-02-10 EP EP03739727A patent/EP1570053A2/en not_active Withdrawn
- 2003-02-10 JP JP2003568031A patent/JP2005526498A/en not_active Withdrawn
- 2003-02-10 CA CA002476237A patent/CA2476237A1/en not_active Abandoned
- 2003-02-10 WO PCT/US2003/004001 patent/WO2003068916A2/en not_active Application Discontinuation
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6737266B1 (en) * | 1999-10-01 | 2004-05-18 | 3M Innovative Properties Company | Devices and methods for microorganism detection |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006055371A2 (en) * | 2004-11-16 | 2006-05-26 | Alexion Pharmaceuticals, Inc. | Functional screening of antibody libraries |
WO2006055371A3 (en) * | 2004-11-16 | 2006-08-17 | Alexion Pharma Inc | Functional screening of antibody libraries |
CN109906267A (en) * | 2016-10-12 | 2019-06-18 | 连王·罗斯 | Miniature organism reactor assemblies |
Also Published As
Publication number | Publication date |
---|---|
WO2003068916A3 (en) | 2005-07-14 |
AU2003210949A1 (en) | 2003-09-04 |
EP1570053A2 (en) | 2005-09-07 |
JP2005526498A (en) | 2005-09-08 |
CA2476237A1 (en) | 2003-08-21 |
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