WO2003066890A3 - Method for determining the methylation pattern of dna - Google Patents

Method for determining the methylation pattern of dna Download PDF

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Publication number
WO2003066890A3
WO2003066890A3 PCT/EP2003/001035 EP0301035W WO03066890A3 WO 2003066890 A3 WO2003066890 A3 WO 2003066890A3 EP 0301035 W EP0301035 W EP 0301035W WO 03066890 A3 WO03066890 A3 WO 03066890A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
determining
sequence
methylation pattern
uracil
Prior art date
Application number
PCT/EP2003/001035
Other languages
German (de)
French (fr)
Other versions
WO2003066890A2 (en
Inventor
Jochen Muth
Andreas Kappel
Heike Behrensdorf
Original Assignee
Nanogen Recognomics Gmbh
Jochen Muth
Andreas Kappel
Heike Behrensdorf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanogen Recognomics Gmbh, Jochen Muth, Andreas Kappel, Heike Behrensdorf filed Critical Nanogen Recognomics Gmbh
Priority to AU2003210200A priority Critical patent/AU2003210200A1/en
Publication of WO2003066890A2 publication Critical patent/WO2003066890A2/en
Publication of WO2003066890A3 publication Critical patent/WO2003066890A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Abstract

The invention relates to a method for determining the methylation pattern in DNA-sequences whereby non-methylated cytosine in the DNA-sequence is chemically converted to uracil and the thus treated DNA-sequence is hybridised by a detector-DNA-sequence. The hybrids are fixed in a locally dissolved manner on a surface. Subsequently, the detection of guanine/uracil-mispairing occurs by using substances detected by said mispairings.
PCT/EP2003/001035 2002-02-04 2003-02-03 Method for determining the methylation pattern of dna WO2003066890A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003210200A AU2003210200A1 (en) 2002-02-04 2003-02-03 Method for determining the methylation pattern of dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2002104566 DE10204566A1 (en) 2002-02-04 2002-02-04 Method for determining the methylation pattern of DNA
DE10204566.6 2002-02-04

Publications (2)

Publication Number Publication Date
WO2003066890A2 WO2003066890A2 (en) 2003-08-14
WO2003066890A3 true WO2003066890A3 (en) 2004-03-25

Family

ID=27588353

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/001035 WO2003066890A2 (en) 2002-02-04 2003-02-03 Method for determining the methylation pattern of dna

Country Status (3)

Country Link
AU (1) AU2003210200A1 (en)
DE (1) DE10204566A1 (en)
WO (1) WO2003066890A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004002257B4 (en) * 2004-01-09 2006-11-09 Epigenomics Ag Method for studying cytosine methylations in DNA using DNA repair enzymes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028498A2 (en) * 1997-11-27 1999-06-10 Epigenomics Gmbh Method for producing complex dna methylation fingerprints
WO1999039003A1 (en) * 1998-01-30 1999-08-05 Genzyme Corporation Method for detecting and identifying mutations
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
EP1041160A1 (en) * 1997-07-31 2000-10-04 The Institute Of Physical & Chemical Research Methods for detecting mutation in base sequence
WO2001044504A2 (en) * 1999-12-17 2001-06-21 Astrazeneca Ab Method for detecting methylated cpg-containing nucleic acid

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
DE19853398C1 (en) * 1998-11-19 2000-03-16 Epigenomics Gmbh Identification of 5-methylcytosine positions in genomic DNA by chemical modification, amplification and heteroduplex formation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
EP1041160A1 (en) * 1997-07-31 2000-10-04 The Institute Of Physical & Chemical Research Methods for detecting mutation in base sequence
WO1999028498A2 (en) * 1997-11-27 1999-06-10 Epigenomics Gmbh Method for producing complex dna methylation fingerprints
WO1999039003A1 (en) * 1998-01-30 1999-08-05 Genzyme Corporation Method for detecting and identifying mutations
WO2001044504A2 (en) * 1999-12-17 2001-06-21 Astrazeneca Ab Method for detecting methylated cpg-containing nucleic acid

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GOTOH M ET AL: "Rapid method for detection of point mutations using mismatch binding protein (MutS) and an optical biosensor", GENETIC ANALYSIS: BIOMOLECULAR ENGINEERING, ELSEVIER SCIENCE PUBLISHING, US, vol. 14, no. 2, 1 July 1997 (1997-07-01), pages 47 - 50, XP004126266, ISSN: 1050-3862 *
REIN ET AL: "Identifying 5-methylcytosine and related modifications in DNA genomes", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 10, 1998, pages 2255 - 2264, XP002143106, ISSN: 0305-1048 *
WAGNER R ET AL: "MUTATION DETECTION USING IMMOBILIZED MISMATCH BINDING PROTEIN (MUTS)", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 23, no. 19, 1995, pages 3944 - 3948, XP002916167, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2003066890A2 (en) 2003-08-14
AU2003210200A1 (en) 2003-09-02
DE10204566A1 (en) 2003-08-14

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