WO2003065043A3 - Identification and relative quantification of proteins - Google Patents

Identification and relative quantification of proteins Download PDF

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Publication number
WO2003065043A3
WO2003065043A3 PCT/GB2003/000346 GB0300346W WO03065043A3 WO 2003065043 A3 WO2003065043 A3 WO 2003065043A3 GB 0300346 W GB0300346 W GB 0300346W WO 03065043 A3 WO03065043 A3 WO 03065043A3
Authority
WO
WIPO (PCT)
Prior art keywords
identification
proteins
relative quantification
relates
orientated
Prior art date
Application number
PCT/GB2003/000346
Other languages
French (fr)
Other versions
WO2003065043A2 (en
Inventor
Hendrik Nubert
Andrew Thomas Kicman
Ray Kruse Iles
Original Assignee
King S College London An Inst
Queen Mary University Of Londo
Hendrik Nubert
Andrew Thomas Kicman
Ray Kruse Iles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0202013A external-priority patent/GB0202013D0/en
Priority claimed from GB0227811A external-priority patent/GB0227811D0/en
Application filed by King S College London An Inst, Queen Mary University Of Londo, Hendrik Nubert, Andrew Thomas Kicman, Ray Kruse Iles filed Critical King S College London An Inst
Publication of WO2003065043A2 publication Critical patent/WO2003065043A2/en
Publication of WO2003065043A3 publication Critical patent/WO2003065043A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates

Abstract

The invention relates to the identification and relative quantification of proteins, especially through matrix-assisted laser desorption ionisation mass spectrometry. In particular it relates to the identification of analytes captured on orientated antibodies.
PCT/GB2003/000346 2002-01-29 2003-01-28 Identification and relative quantification of proteins WO2003065043A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0202013A GB0202013D0 (en) 2002-01-29 2002-01-29 Identification and relative quantification of proteins
GB0202013.9 2002-01-29
GB0227811A GB0227811D0 (en) 2002-11-28 2002-11-28 Identification and relative quantification of proteins
GB0227811.7 2002-11-28

Publications (2)

Publication Number Publication Date
WO2003065043A2 WO2003065043A2 (en) 2003-08-07
WO2003065043A3 true WO2003065043A3 (en) 2004-02-19

Family

ID=27665355

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/000346 WO2003065043A2 (en) 2002-01-29 2003-01-28 Identification and relative quantification of proteins

Country Status (1)

Country Link
WO (1) WO2003065043A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066275A1 (en) * 2007-11-22 2009-05-28 Dublin City University A method of immobilising biological molecules to a support and products thereof
GB201305317D0 (en) 2013-03-22 2013-05-08 Iles Raymond K Prenatal screening for fetal abnormalities and disorders of pregnancy
US20200018769A1 (en) * 2017-03-31 2020-01-16 Samdi Tech, Inc. Articles and methods involving detection of binding
CN117607463B (en) * 2024-01-23 2024-04-09 杭州华得森生物技术有限公司 EGFR (epidermal growth factor receptor) immune chromogenic detection reagent for circulating tumor cells
CN117630370B (en) * 2024-01-23 2024-04-09 杭州华得森生物技术有限公司 HER2 positive CTC (CTC) immunochromatography detection reagent

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998059360A1 (en) * 1997-06-20 1998-12-30 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
WO2000034474A2 (en) * 1998-12-07 2000-06-15 Zymogenetics, Inc. Growth factor homolog zvegf3
WO2002061047A2 (en) * 2001-02-01 2002-08-08 Ciphergen Biosystems, Inc. Improved methods for protein identification, characterization and sequencing by tandem mass spectrometry

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998059360A1 (en) * 1997-06-20 1998-12-30 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
WO2000034474A2 (en) * 1998-12-07 2000-06-15 Zymogenetics, Inc. Growth factor homolog zvegf3
WO2002061047A2 (en) * 2001-02-01 2002-08-08 Ciphergen Biosystems, Inc. Improved methods for protein identification, characterization and sequencing by tandem mass spectrometry

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BROCKMAN ADAM H ET AL: "New immobilization chemistry for probe affinity mass spectrometry", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 10, no. 13, 1996, pages 1688 - 1692, XP009021512, ISSN: 0951-4198 *
LIANG X ET AL: "On-probe immunoaffinity extraction by matrix-assisted laser desorption/ionization mass spectrometry.", ANALYTICAL CHEMISTRY. UNITED STATES 1 FEB 1998, vol. 70, no. 3, 1 February 1998 (1998-02-01), pages 498 - 503, XP002263287, ISSN: 0003-2700 *
LU B ET AL: "Oriented immobilization of antibodies and its applications in immunoassays and immunosensors.", THE ANALYST. ENGLAND MAR 1996, vol. 121, no. 3, March 1996 (1996-03-01), pages 29R - 32R, XP009022000, ISSN: 0003-2654 *
NEUBERT HENDRIK ET AL: "Enhanced affinity capture MALDI-TOF MS: orientation of an immunoglobulin G using recombinant protein G.", ANALYTICAL CHEMISTRY. UNITED STATES 1 AUG 2002, vol. 74, no. 15, 1 August 2002 (2002-08-01), pages 3677 - 3683, XP002263289, ISSN: 0003-2700 *
PETER J F ET AL: "A general strategy for epitope mapping by direct MALDI-TOF mass spectrometry using secondary antibodies and cross-linking.", ANALYTICAL CHEMISTRY. UNITED STATES 15 AUG 2001, vol. 73, no. 16, 15 August 2001 (2001-08-15), pages 4012 - 4019, XP002263286, ISSN: 0003-2700 *
ZHAO Y ET AL: "Mapping protein-protein interactions by affinity-directed mass spectrometry.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. UNITED STATES 30 APR 1996, vol. 93, no. 9, 30 April 1996 (1996-04-30), pages 4020 - 4024, XP002263288, ISSN: 0027-8424 *

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