WO2003060114A3 - Procede de desintegration a l'aide de champs electriques alternatifs en continu, a modification brusque d'intensite - Google Patents

Procede de desintegration a l'aide de champs electriques alternatifs en continu, a modification brusque d'intensite Download PDF

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Publication number
WO2003060114A3
WO2003060114A3 PCT/EP2003/000128 EP0300128W WO03060114A3 WO 2003060114 A3 WO2003060114 A3 WO 2003060114A3 EP 0300128 W EP0300128 W EP 0300128W WO 03060114 A3 WO03060114 A3 WO 03060114A3
Authority
WO
WIPO (PCT)
Prior art keywords
field strength
electric fields
alternating electric
strength changes
continuously alternating
Prior art date
Application number
PCT/EP2003/000128
Other languages
German (de)
English (en)
Other versions
WO2003060114A2 (fr
Inventor
Frank Vitzthum
Juergen Bernhagen
Herwig Brunner
Original Assignee
Fraunhofer Ges Forschung
Frank Vitzthum
Juergen Bernhagen
Herwig Brunner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Ges Forschung, Frank Vitzthum, Juergen Bernhagen, Herwig Brunner filed Critical Fraunhofer Ges Forschung
Priority to AU2003215534A priority Critical patent/AU2003215534A1/en
Publication of WO2003060114A2 publication Critical patent/WO2003060114A2/fr
Publication of WO2003060114A3 publication Critical patent/WO2003060114A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

Abstract

L'invention concerne un procédé de désintégration de matériau cellulaire biologique pour extraire des substances cellulaires ou transférer des principes actifs dans le matériau cellulaire biologique à l'aide de champs alternatifs en continu, à modification brusque d'intensité.
PCT/EP2003/000128 2002-01-15 2003-01-09 Procede de desintegration a l'aide de champs electriques alternatifs en continu, a modification brusque d'intensite WO2003060114A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003215534A AU2003215534A1 (en) 2002-01-15 2003-01-09 Disruption method by means of continuously alternating electric fields with abrupt field strength changes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2002101174 DE10201174A1 (de) 2002-01-15 2002-01-15 Desintegrationsverfahren mittels kontinuierlich alternierender elektrischer Felder mit abrupter Feldstärkeänderung
DE10201174.5 2002-01-15

Publications (2)

Publication Number Publication Date
WO2003060114A2 WO2003060114A2 (fr) 2003-07-24
WO2003060114A3 true WO2003060114A3 (fr) 2003-12-31

Family

ID=7712115

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/000128 WO2003060114A2 (fr) 2002-01-15 2003-01-09 Procede de desintegration a l'aide de champs electriques alternatifs en continu, a modification brusque d'intensite

Country Status (3)

Country Link
AU (1) AU2003215534A1 (fr)
DE (1) DE10201174A1 (fr)
WO (1) WO2003060114A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202011004177U1 (de) * 2011-03-18 2012-06-25 Hugo Vogelsang Maschinenbau Gmbh Vorrichtung zur elektrischen Desintegration
DE202013005125U1 (de) * 2013-06-04 2014-09-05 Hugo Vogelsang Maschinenbau Gmbh Vorrichtung zur elektrischen Desintegration von Zellverbänden

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999062592A1 (fr) * 1998-06-03 1999-12-09 Genetronics, Inc. TECHNIQUE D'ELECTROPORATION A FLUX TRAVERSANT POUR THERAPIE GENIQUE $i(EX VIVO)
US6043066A (en) * 1997-09-04 2000-03-28 Mangano; Joseph A. Cell separation using electric fields
WO2000049173A2 (fr) * 1999-02-15 2000-08-24 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Procede et systeme de support d'echantillon pour la separation et l'enrichissement de substances in situ
US6117660A (en) * 1997-06-10 2000-09-12 Cytopulse Sciences, Inc. Method and apparatus for treating materials with electrical fields having varying orientations

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117660A (en) * 1997-06-10 2000-09-12 Cytopulse Sciences, Inc. Method and apparatus for treating materials with electrical fields having varying orientations
US6043066A (en) * 1997-09-04 2000-03-28 Mangano; Joseph A. Cell separation using electric fields
WO1999062592A1 (fr) * 1998-06-03 1999-12-09 Genetronics, Inc. TECHNIQUE D'ELECTROPORATION A FLUX TRAVERSANT POUR THERAPIE GENIQUE $i(EX VIVO)
WO2000049173A2 (fr) * 1999-02-15 2000-08-24 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Procede et systeme de support d'echantillon pour la separation et l'enrichissement de substances in situ

Also Published As

Publication number Publication date
DE10201174A1 (de) 2003-07-31
WO2003060114A2 (fr) 2003-07-24
AU2003215534A1 (en) 2003-07-30

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