WO2003056003A1 - Amelioration de la stabilite d'enzymes - Google Patents

Amelioration de la stabilite d'enzymes Download PDF

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Publication number
WO2003056003A1
WO2003056003A1 PCT/AU2002/001487 AU0201487W WO03056003A1 WO 2003056003 A1 WO2003056003 A1 WO 2003056003A1 AU 0201487 W AU0201487 W AU 0201487W WO 03056003 A1 WO03056003 A1 WO 03056003A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
amino acid
xylanase
positively
uncharged
Prior art date
Application number
PCT/AU2002/001487
Other languages
English (en)
Inventor
Khawar Sohail Siddiqui
Ricardo Cavicchioli
Original Assignee
Unisearch Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unisearch Limited filed Critical Unisearch Limited
Priority to AU2002335938A priority Critical patent/AU2002335938A1/en
Publication of WO2003056003A1 publication Critical patent/WO2003056003A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03001—Alkaline phosphatase (3.1.3.1)

Abstract

Cette invention porte sur une enzyme et sur des procédés de production d'une enzyme pouvant effectuer le clivage de liaisons β-1,4-xylosidiques de xylane. Cette enzyme comprend une xylanase qui a été modifiée par l'introduction d'une molécule neutre ou chargée positivement liée à une chaîne latérale d'un acide aminé de la xylanase et/ou à un acide aminé C-terminal de la xylanase. L'enzyme agit à une température élevée et/ou présente une demi-vie prolongée par rapport à la xylanase non modifiée correspondante.
PCT/AU2002/001487 2001-12-21 2002-11-01 Amelioration de la stabilite d'enzymes WO2003056003A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002335938A AU2002335938A1 (en) 2001-12-21 2002-11-01 Improvement in enzyme stability

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPR9688A AUPR968801A0 (en) 2001-12-21 2001-12-21 Improvements in enzyme stability
AUPR9688 2001-12-21

Publications (1)

Publication Number Publication Date
WO2003056003A1 true WO2003056003A1 (fr) 2003-07-10

Family

ID=3833301

Family Applications (6)

Application Number Title Priority Date Filing Date
PCT/AU2002/001487 WO2003056003A1 (fr) 2001-12-21 2002-11-01 Amelioration de la stabilite d'enzymes
PCT/AU2002/001483 WO2003055999A1 (fr) 2001-12-21 2002-11-01 Enzyme modifie et son procede de modification
PCT/AU2002/001484 WO2003056000A1 (fr) 2001-12-21 2002-11-01 Enzyme modifiee et procede de modification
PCT/AU2002/001488 WO2003056004A1 (fr) 2001-12-21 2002-11-01 Ameliorations de la thermolabilite d'enzymes
PCT/AU2002/001486 WO2003056002A1 (fr) 2001-12-21 2002-11-01 Stabilite d'amylase amelioree
PCT/AU2002/001485 WO2003056001A1 (fr) 2001-12-21 2002-11-01 Enzymes modifiees et procedes de modification

Family Applications After (5)

Application Number Title Priority Date Filing Date
PCT/AU2002/001483 WO2003055999A1 (fr) 2001-12-21 2002-11-01 Enzyme modifie et son procede de modification
PCT/AU2002/001484 WO2003056000A1 (fr) 2001-12-21 2002-11-01 Enzyme modifiee et procede de modification
PCT/AU2002/001488 WO2003056004A1 (fr) 2001-12-21 2002-11-01 Ameliorations de la thermolabilite d'enzymes
PCT/AU2002/001486 WO2003056002A1 (fr) 2001-12-21 2002-11-01 Stabilite d'amylase amelioree
PCT/AU2002/001485 WO2003056001A1 (fr) 2001-12-21 2002-11-01 Enzymes modifiees et procedes de modification

Country Status (2)

Country Link
AU (1) AUPR968801A0 (fr)
WO (6) WO2003056003A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011203101B2 (en) * 2004-12-22 2012-11-08 Novozymes A/S Enzymes for starch processing
ES2543131T3 (es) 2004-12-22 2015-08-14 Novozymes North America, Inc. Enzimas para tratamiento de almidón
US8841091B2 (en) 2004-12-22 2014-09-23 Novozymes Als Enzymes for starch processing
CN106244645B (zh) * 2016-08-31 2020-07-10 保龄宝生物股份有限公司 一种循环利用米曲霉菌体生产低聚果糖的方法
US11932860B2 (en) 2017-10-03 2024-03-19 Kikkoman Corporation Method for producing alkaline phosphatase, alkaline phosphatase obtained using said method, and vector and transformant for production thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995009909A1 (fr) * 1993-10-04 1995-04-13 Novo Nordisk A/S Preparation comportant une enzyme modifiee
WO1997024421A2 (fr) * 1995-12-29 1997-07-10 The Procter & Gamble Company Compositions detergentes comprenant des enzymes immobilisees
US5759840A (en) * 1996-09-09 1998-06-02 National Research Council Of Canada Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4362818A (en) * 1981-02-17 1982-12-07 Miles Laboratories, Inc. Acylation of Mucor pusillus microbial rennet enzyme
GB2225324B (en) * 1988-11-25 1993-02-03 Branko Kozulic Stabilization of glycoproteins
KR100204837B1 (ko) * 1989-04-28 1999-06-15 기스트 브로카데스 나암로제 베노오트하프 카르복실 에스테라제의 안정화
ATE92953T1 (de) * 1989-10-13 1993-08-15 Novo Nordisk As Fluessiges enzymatisches reinigungsmittel.
JP3125428B2 (ja) * 1991-03-28 2001-01-15 和光純薬工業株式会社 修飾酵素
AU1946895A (en) * 1994-03-28 1995-10-17 Novo Nordisk A/S A modified cellulase and an enzyme preparation comprising a modified cellulase
FR2736065B1 (fr) * 1995-06-29 1997-09-19 Commissariat Energie Atomique Phosphatases alcalines bacteriennes modifiees et leurs applications.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995009909A1 (fr) * 1993-10-04 1995-04-13 Novo Nordisk A/S Preparation comportant une enzyme modifiee
WO1997024421A2 (fr) * 1995-12-29 1997-07-10 The Procter & Gamble Company Compositions detergentes comprenant des enzymes immobilisees
US5759840A (en) * 1996-09-09 1998-06-02 National Research Council Of Canada Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHAUTHAIWALE J., RAO M.: "Specific interaction of guanidine hydrochloride with essential carboxyl group of xylanase from alkalothermophilic bacillus sp.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 191, no. 3, 31 March 1993 (1993-03-31), pages 922 - 927 *
RAO M., KHADILKAR S., DESHPANDE V.: "Chemical modification of xylanase from Chainia sp. (NCL 82.5.1)", BIOTECHNOLOGY LETTERS, vol. 17, no. 6, 1995, pages 589 - 592 *
WEN L., MIAO Z.W., QING W.D.: "Chemical modification of xylanase from trichosporon cutaneum shows the presence of carboxyl groups and cysteine residues essential for enzyme activity", JOURNAL OF PROTEIN CHEMISTRY, vol. 18, no. 6, August 1999 (1999-08-01), pages 677 - 686 *

Also Published As

Publication number Publication date
AUPR968801A0 (en) 2002-01-24
WO2003056001A1 (fr) 2003-07-10
WO2003056000A1 (fr) 2003-07-10
WO2003056004A1 (fr) 2003-07-10
WO2003055999A1 (fr) 2003-07-10
WO2003056002A1 (fr) 2003-07-10

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